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Sri Utaminingsih
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Journal : Food Scientia: Journal of Food Science and Technology

 1 
Deteksi DNA Spesies pada Produk Makanan Olahan Daging Ayam Menggunakan Gen GH (Growth Hormone) sebagai Penanda Molekuler Sophian, Alfi; Utaminingsih, Sri; Yenita, Yenita; Purwaningsih, Ratna
Food Scientia : Journal of Food Science and Technology Vol 4 No 1 (2024): January - June
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33830/fsj.v4i1.6358.2024

Abstract

The species DNA detection test in processed chicken meat food products using genetic growth hormone (GH) gene markers is a novel approach for authenticity test regarding the presence of a particular species in a product. This research aimed to test the ability of the GH genetic marker to detect the presence of chicken species in processed chicken meat food products. The method used in this research is a molecular biology method using real-time PCR with the SYBR Green kit. The genetic marker primers used were designed independently from the NCBI website. Based on DNA isolation data, the DNA concentration value is 43.60 ng/µL – 45.20 ng/µL with an average of 44.11. The purity value of isolated DNA is 1.855 – 1.925 with an average of 1.906. Chicken species DNA was detected in all samples using real-time PCR analysis, with a Ct value of 24.50 and a Tm value of 78.10. However, the Ct and Tm values were not detected in the negative control (NTC) and specificity (internal) control samples. In the positive control LOD, the Ct value was detected at 27.20 with a Tm value of 79.40. In the positive control, a Ct value of 21.10 and a Tm value of 78.10 were detected. The results indicate that all samples contained the GH gene, and hence, chicken species DNA was detected in all samples. This suggests that the GH genetic marker can be potentially used for species DNA identification testing.
Co-Authors A.A. Ketut Agung Cahyawan W Abd. Chaidir Marasabessy Abdul Latif Abdul Razak Abdurrahman, Rohman Adelina Hasyim Afriyanti, Susi Agustini, Ferina Ahlami, Mami Ahmad Hariyadi, Ahmad Akhirudin, Akhirudin Alam, Sirrul Alfatonah, Lutfiah Alisyani, Siti Ambarita, Junita Hotnida AMILUDIN, RIPKI Aniq KHB, Moh Arif Hidayat Arif Munandar Asih, Windi Avifah, Hananda Belia, Gones C. Bien, Jonna Karla Choirul Amin Edris Zamroni Eka Zuliana Eko Darmanto Elitha, Tiara ENDRI ENDRI Erik Aditia Ismaya Ersam Mahendrawan Evi Ekawati Fathurrohman, Irfai Fauziah, Nanda Sifa Fauziah, Shilva Nurmayanti Febiyansyah, Febiyansyah Ferry Kurniawan, Ferry Fery Kurniawan Fina Fakhriyah Gunawan Setiadi Habsari, Arika Febriana Hala, Ahmad Irfan Hardika, Fiki Fandi HARIYANTO HARIYANTO Haryanti, Amelia Hendri Hendri Henry Suryo Bintoro Hilmy Aliriad Hutagalung, Jeremi Ika Oktavianti, Ika Indayani, Rin Indriani, Iin Irfai Fathurohman, Irfai Jayanti Putri, Jayanti Juniati Juniati Kartika, Deny Dwi Khakim -, Khakim Khamdun khussa'adah, Mifta Kironoratri, Lingtang Kurnia, Jenia Lailiah, Lailiah lestari, Yulita Puji Lovika Ardana Riswari Lukmana, Andri Mahanita, Byarvega Majda, Nadia Ela Maretta, Loula Maulidiyah, Siti Robiatul Mohammad Kanzunnudin, Mohammad Muhamad Iqbal Muhamad Rizki Muhammad Akbar Muliani, Anggita Murtono Murtono Na'im, Salsabila Nailul Muna, Salsabila Nanik Istika Wati, Nanik Istika Napasti, Friny Nasiroh, Nasiroh Nining Purwaningsih Nisa, Santi Khasiatin Nofeca, Ysta Nugrahaeni, Titi Santi Nurdin, Tammi Nasuha Nurhadi, Moch Nurlaili, Lili Nurzaman, E PRASETYAWAN, ENGGAR Prastini, Endang Pratiwi, Estherini Heratity Puji Lestari, Yulita Puspita, Devthania Arum Putri, Mas Fierna Janvierna Lusie Putriani, Faniya Rachmawaty, Shella Raharjo, Mohamad Topan Ramadhan, Luky Fatur Rani Setiawaty Ratna Purwaningsih Ratri Rahayu Retno Wulandari Richma Hidayati Riska Putri Rosikhatul Ilmiyah, Rosikhatul Said Permana, Putut Said, Moh. Rohmat Saiful Anwar Samudera, Tirta SANTOSO SANTOSO Santoso, Deni Agung SESRIYANI, LODYA Setiawati Setiawati Shella Rachmawaty Shofwani, Siti Aniqoh Shufi, Muhammad Iqbal Silalahi, Kristina Siswandi, R. Dede Siti Masfuah Siti Rahayu Somanto, Djoko Sophian, Alfi Sri Astuti sri murtini . Sri Surachmi Su'ad, Su'ad Suad, Suad Su’ad, Su’ad Subagio, Muhamad Harry Sucipto Sucipto Sumaji Sumanto, Djoko Sumaryati, Ismi Suwandi Syariati, Tri Puput Trihandayani, Estiningsih Tupari Tupari Ulfah, Mita Kurnia Umami, Retno Rhisalatul Utami, Sofia Dyah Utomo, Slamet Utomo, Slamet W, Sri Surachmi Wahyu Kurniawan Wawan Shokib Rondli Widyati, Wiwin Yanto Sugianto Yenita . Yuliana, Lilik Yulita Pujilestari Zaenap, Siti Zehan, Zehan Zulfah, Ummu Zulkifli Zulkifli