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Ayu Wahyuni
Fakultas Kehutanan Universitas Tanjungpura. Jln Imam Bonjol Pontianak 78124
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Journal : Jurnal Biogenerasi

 1 
PEWARNAAN GRAM ISOLAT BAKTERI DARI LIMBAH BIOMEDIS CAIR RUMAH SAKIT UNHAS DENGAN METODE ZIEHL NEELSEN Wulandhani, Suci; Wahyuni, Ayu; Hasyim, Ahmad; Misnarliah
Jurnal Biogenerasi Vol. 10 No. 1 (2024): Volume 10 Nomor 1, Agustus 2024 - Februari 2025
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i1.4386

Abstract

Hospitals as one of the health service institutions that provide inpatient, outpatient and emergency services, in their implementation can produce waste in solid, liquid or gas form which can pollute the environment. Hospital waste water is an accumulation of domestic waste and liquid biomedical waste with high levels of organic pollutants, so it can be treated biologically. Liquid biomedical waste contains many microorganisms where the presence of microorganisms, in this case bacteria, can be used as an indicator of waste management and unfavorable environmental conditions. One waste water treatment technology that is safe for the environment is using bacteria that have the potential to act as decomposers in the biodegradation process. To obtain bacteria that have the potential to act as natural decomposers, you can isolate the waste itself. The bacteria found in liquid biomedical waste make it possible to find a group of non-pathogenic hydrolytic bacteria where this group of bacteria can produce various enzymes that are capable of degrading liquid biomedical waste. Gram staining is a method to differentiate between types or spesies of bacteria namely gram-positive bacteria and gram-negative bacteria. The aim of this study was to identify bacteria using Ziehl Neelsen gram staining. The results of the research showed that there were two bacterial isolates that were gram negative, namely isolate codes UH1.1 and UH1.2 (bacillus) form characterized by the appearance of brick red cells and five isolates that were gram positive, namely isolate codes UH1.3, UH2.1 (bacillus) UH2.2, UH2.3 and UH3.1 (coccus form) are characterized by the appearance of purple cells, the difference in color is due to differences in the composition of the cell walls of each bacteria.
PEWARNAAN GRAM ISOLAT BAKTERI DARI LIMBAH BIOMEDIS CAIR RUMAH SAKIT UNHAS DENGAN METODE ZIEHL NEELSEN Wulandhani, Suci; Wahyuni, Ayu; Hasyim, Ahmad; Misnarliah
Jurnal Biogenerasi Vol. 10 No. 1 (2024): Volume 10 Nomor 1, Agustus 2024 - Februari 2025
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i1.4386

Abstract

Hospitals as one of the health service institutions that provide inpatient, outpatient and emergency services, in their implementation can produce waste in solid, liquid or gas form which can pollute the environment. Hospital waste water is an accumulation of domestic waste and liquid biomedical waste with high levels of organic pollutants, so it can be treated biologically. Liquid biomedical waste contains many microorganisms where the presence of microorganisms, in this case bacteria, can be used as an indicator of waste management and unfavorable environmental conditions. One waste water treatment technology that is safe for the environment is using bacteria that have the potential to act as decomposers in the biodegradation process. To obtain bacteria that have the potential to act as natural decomposers, you can isolate the waste itself. The bacteria found in liquid biomedical waste make it possible to find a group of non-pathogenic hydrolytic bacteria where this group of bacteria can produce various enzymes that are capable of degrading liquid biomedical waste. Gram staining is a method to differentiate between types or spesies of bacteria namely gram-positive bacteria and gram-negative bacteria. The aim of this study was to identify bacteria using Ziehl Neelsen gram staining. The results of the research showed that there were two bacterial isolates that were gram negative, namely isolate codes UH1.1 and UH1.2 (bacillus) form characterized by the appearance of brick red cells and five isolates that were gram positive, namely isolate codes UH1.3, UH2.1 (bacillus) UH2.2, UH2.3 and UH3.1 (coccus form) are characterized by the appearance of purple cells, the difference in color is due to differences in the composition of the cell walls of each bacteria.
Co-Authors Abriyani, Ermi Afriliany, Syerli Putri Ahmad Hasyim Akbar, M Ali Ali, Muh. Yunus Anis, Madhan Arnentis Asni, Nur Asni Aswan, Dara Mutiara Avianita Rachmawati Azaria, Liza Azhari, Nurfidia Budiarti, Reni Sulistiyoning Cut Fauziah Damayanti, Ervina Daniel Happy Putra Dedi Supendra Devi Lorenza, Adella E, Muh. Iksan Ekariana S Pandia Eldarni Fadhelina, Nishbah Fairish, Novi Lavly Febblina Daryanes Febriana, Nana Fitri, Dwi Hafiz Ardian Halimatun, Futria Hariani, Indri Harlis Harlis Hartana, Davina Dewi Hasami, M Hayatun Nufus hendri, nofri Hidayah, Himyatul Hidayah, Himyatul Nur Hilwa, Cut Ilham, Ramdani Nur Indriaty Indriaty, Indriaty Irda Sayuti Irmanda, Reva Putri Iswan Dewantara Jayanthi , Sri Juwantara, Ridho Agung Khairunnisa Khairunnisa Khasanah, Nurul Uswatun Lambertus Langga Lanontji, Muryanto Lestari, Indah Lepi Ma'rupah, Ma'rupah Marjanah Marjanah, Marjanah Maurizkya, Khesya Shafira Mawardi, Abdul Latif Misnarliah Mistar, Johaidah Muliana, Syeifa Murniati . Mursyidah Mursyidah Musayyadah Muslimin Muslimin Muthaqimah, Yasinta Vivia Nabila, Alya Maulida Nasution, Hasbi Assidiqi Natalia, Desfaur Nesah, Irma Nirwana, Iqrya Nur Rahmah Nur, Siti Fadhilah Nurfadhillah, Septy Nurhasanah, Risky Nurhasnah Manurung, Nurhasnah Nurmala Sari Nursamsu Nursamsu Pepayosa, Enca Praptini Yulianti, Praptini Pratiwi, Salmi Indah Putri, Dinda Revalina Rahmati Rahmati Raihan, Muhammad Ade Rifni, Azira Rony, Zahara Tussoleha Sari, Muliani Tiana Sarjani, Tri Mustika Saroh, May Sinaga, Juwita Br Sri Jayanthi, Sri Subariyanto, Subariyanto Sukmawati, Cut Syahfitri, Nabila Tendri, Muslimin Ti Aisyah Utami, Alma Yulia Wahyudi Umar Wulandhani, Suci Yeni Agustina, Yeni