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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vegetalika Jurnal Ilmu Pertanian Agroland : Jurnal Ilmu-Ilmu Pertanian Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology International Journal of Renewable Energy Development Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Journal of Tropical Soils Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Berkala Ilmiah Pertanian Jurnal Agro Teknologi Tropika Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Sains Tanah AGRIVITA, Journal of Agricultural Science agriTECH Indonesian Journal of Biotechnology Jurnal Tanah dan Air Planta Tropika Pharmacon Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Jurnal Perlindungan Tanaman Indonesia INDONESIAN JOURNAL OF PHARMACY JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Caraka Tani: Journal of Sustainable Agriculture Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Indonesian Food and Nutrition Progress OLDI (Oseanologi dan Limnologi di Indonesia) JURNAL PERTANIAN jurnal Ilmu Pertanian (Agricultural Science) Jurnal Tanah dan Air (Soil and Water Journal) Kaunia: Integration and Interconnection of Islam and Science Journal Engagement: Jurnal Pengabdian Kepada Masyarakat Indonesian Journal of Pharmacology and Therapy

JAKA WIDADA

Departemen Mikrobiologi Pertanian, Fakultas Pertanian, Universitas Gadjah Mada

Author-ID : 414939

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Social Sciences Veterinary

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Pengaruh Perbedaan Metode Ekstraksi Metabolit Sekunder Streptomyces sp. GMR22 terhadap Toksisitas pada Sel BHK-21 Diani Mentari; Mirtani Naima; Riska Wulansari; Jaka Widada; Tri Rini Nuringtyas; Tri Wibawa; Nastiti Wijayanti
Pharmacon: Jurnal Farmasi Indonesia Vol 16, No 1 (2019)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/pharmacon.v16i1.8032

Streptomyces sp. GMR22 is local isolate from Wanagama 1 Forest in Yogyakarta. They have the potential to be developed to produce active compounds because have PKS and NRPS genes.The active compounds from isolation are strongly influenced by various factors, one of them is extraction techniques. Effect difference of extraction technique will be affected by the quality of secondary metabolites produced.The purpose of this study was to compare the cytotoxicity effects of secondary metabolites of Streptomyces sp. GMR22 which have extracted with different stages from previous studies. The extraction technique was carried out by multilevel separatory funnel extraction methods, which was first extracted using non-polar solvent (n-hexane) and then extracted using semi-polar solvent (ethyl acetate). This research is important because in previous studies (separatory funnel only extracted using ethyl acetate) with the use of the lowest concentration in the dengue virus antiviral test (further test) caused 100% of deaths in BHK-21 cells.This study indicate that multilevel extraction result in lower CC50 value than previous studies. There are 49.160 µl/ml (n-hexane extract) and 284.56 µl/ml (ethyl acetate extract) while water extract is 464,38 µl/ml. FTIR compound analysis show that the three extracts produced have different spectrum patterns, especially in the n-hexane and ethyl acetate extract. Value of CC50 is not too high, it is expected that the secondary metabolites contained in the extracts can be used for further analysis such as antiviral testing because it is safe for normal host cells such as BHK-21 cells

Pemanfaatan Limbah Biomassa Sawit Ramah Lingkungan Denny Irawati; Ganis Lukmandaru; Joko Sulistyo; Sigit Sunarta; Tomy Listyanto; Jaka Widada; Nunuk Supriyatno; Yose Rizal
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 6, No 4 (2020): Desember
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Dalam rangka memenuhi kebutuhan lahan untuk pembangunan perumahan karyawan, PT Semen Baturaja Tbk. (PTSB) membuka kurang lebih 27 hektar lahan yang di dalamnya diperkirakan terdapat kurang lebih 2700 batang tanaman kelapa sawit yang kurang produktif. Pembukaan lahan tersebut akan menghasilkan biomassa sawit yang cukup besar. Salah satu usaha pemanfaatan limbah biomassa sawit adalah dengan mengolahnya menjadikompos. Kompos dapat digunakan untuk rehabilitasi lahan bekas tambang oleh PTSB. Hingga saat ini, PTSB belum memiliki pengalaman di bidang pembuatan kompos. Oleh karena itu, PTSB bekerja sama dengan Fakultas Kehutanan, Universitas Gadjah Mada untuk memproduksi pupuk kompos dari limbah biomassa tanaman sawit. Metode yang digunakan pada kegiatan pengabdian ini adalah sosialisasi, pembuatan kompos secara partisipatif, dan pendampingan di lapangan. Luaran dari kegiatan ini berupa kompos limbah biomassa sawit serta pengetahuan mengenai teknologi proses pembuatan kompos bagi pihak PTSB.Jumlah pupuk kompos yang bisa diperoleh dari delapan gundukan daun kelapa sawit adalah 248,9 ton dan dari batang kelapa sawit sebanyak 1.236,6 ton. Pupuk kompos setelah pengomposan selama dua bulan mempunyai rasio C/N 13,7. Biaya yang dikeluarkan untuk pengomposan adalah Rp591.405.000,00 dengan potensi keuntungan sebesar Rp6.093.232.500,00. Pengetahuan perihal pembuatan kompos berdampak pada penghematan pengeluaran PTSB untuk membeli pupuk guna rehabilitasi lahan serta penanganan permasalahan limbah biomassa.

Karakterisasi dan Deteksi Cepat Bakteri Penyebab Penyakit Darah pada Pisang Nur Edy; Siti Subandiyah; Christanti Sumardiyono; Jaka Widada
Jurnal Perlindungan Tanaman Indonesia Vol 17, No 1 (2011)
Publisher : Universitas Gadjah Mada

Blood disease of banana is one of the most serious banana disease in Indonesia. Although the disease has became the subject of quarantine it eventually spread and found in most provinces in Indonesia. The aim of this research were to identify the blood disease bacterium (BDB) using morphological observation, biochemical assay, pathogenicity testing of hosts range using infectivity titration and rapid detection by Polymerase Chain Reaction (PCR). The results showed that the blood disease bacterium could be differentiated from Ralstonia solanacearum race 2, the causal agent of Moko disease and R. solanacearum tobacco isolates. BDB isolates were not able to hydrolyze gelatin, Tween 80, starch, and were not able to produce nitrite from nitrate. They were only able to produce acid from galactose and glycerol. The pathogenicity test indicated that the BDB was only able to infect the banana/plantain and was not able to infect tomato, eggplant, and chili. Rapid detection using PCR method showed that the 121F/R primers was able to amplify the BDB genome and was not able to amplify the genome of R. solanacearum tobacco isolates.

Identifikasi Fusarium dan Nematoda Parasitik yang Berasosiasi dengan Penyakit Kuning Lada di Kalimantan Barat Suryanti Suryanti; Bambang Hadisutrisno; Mulyadi Mulyadi; Jaka Widada
Jurnal Perlindungan Tanaman Indonesia Vol 19, No 1 (2015)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.16019

Pepper (Piper nigrum), known as the “King of Spices” is one of the most important spices. In the international market, Indonesian pepper has high selling value, due to its flavor characteristics. Pepper yellowing disease is one of the most important disease that caused the decrease of pepper production and become the main problem in the cultivation of pepper in West Kalimantan. This research was conducted to determine the major causal agent of leaf yellowing disease of pepper. The Fusarium associated with diseased plant were isolated from the symptomatic plant and nematodes were isolated from the root with leaf yellowing symptom. The Fusarium isolates were cultured on agar medium, and the nematode was cultured on tomato plant. From diseased pepper in West Kalimantan, it was isolated 4 Fusarium isolates and plant parasitic nematode Meloidogyne. The result showed that H isolate of Fusarium was the most virulent isolate and identified asFusarium solani. The Meloidogyne was identified by the female perenial patern.The nematode was identified as Meloidogyne incognita.INTISARILada (Piper nigrum L.) merupakan salah satu jenis rempah penting yang telah dikenal sebagai “King of Spices”. Di pasar internasional, lada Indonesia mempunyai daya jual tinggi karena cita rasanya yang khas. Salah satu kendala dalam budidaya lada adalah adanya penyakit kuning lada dan sampai saat ini menjadi masalah utama pada pertanaman lada di Kalimantan Barat. Informasi tentang patogen utama yang berinteraksi dengan penyakit kuning lada masih sangat terbatas, sehingga penelitian ini bertujuan untuk mengidentifikasi patogen utama yang berasosiasi dengan penyakit kuning lada. Isolasi Fusarium dilakukan dari batang lada dan isolasi nematoda dilakukan dari akar lada yang bergejala penyakit kuning di Kalimantan Barat. Fusarium hasil isolasi dikulturkan dalam medium agar, sedangkan nematoda hasil isolasi dikulturkan dalam akar tomat. Dari hasil isolasi berhasil didapatkan empat isolat Fusarium dan nematoda Meloidogyne. Identifikasi Fusarium dilakukan secara morfologis dan molekuler, dan identifikasi Meloidogyne dilakukan dengan menggunakan irisan bagian posterior nematoda betina. Dari hasil identifikasi diketahui bahwa patogen yang berasosiasi dengan penyakit kuning lada adalah Fusarium solani dan Meloidogyne incognita.

Interaksi Meloidogyne incognita dan Fusarium solani pada Penyakit Kuning Lada Suryanti Suryanti; Bambang Hadisutrisno; Mulyadi Mulyadi; Jaka Widada
Jurnal Perlindungan Tanaman Indonesia Vol 21, No 2 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.29760

Pepper yellowing disease is one of the most important disease of pepper causing the decrease of pepper production. This research was conducted in the screen house and laboratory to determine the major causal agent of leaf yellowing disease of pepper. Meloidogyne incognita and Fusarium solani were isolated from pepper plantation in West Kalimantan. Pepper seedlings Natar 1 cultivars were planted in sterilized soil collected from pepper plantation in Bengkayang, West Kalimantan. Five months-old seedling were inoculated with M. incognita (1000 larvae of 2nd stadium/plant) and F. solani (50 ml of spore suspension with spore density of 106 spore/ml) in several combinations of time inoculation, i.e., F. solani and then M. incognita, M. incognita and then F. solani, M. incognita together with F. solani, M. incognita only, and F. solani only. The parameters observed were the development of leaf yellowing disease every weeks for five months. The number of gall, and population M. incognita were observed at the end of the observation. The result showed that when M. incognita was inoculated to the roots followed by F. solani, the disease severity and the percentage of plant diseases were higher than those which were infected with F. solani or M. incognita alone. The higher population densities of M. incognita and a number of root gall, had observed on plants inoculated by M. incognita combined with F. solani than plants inoculated by M. incognita and F. solani alone. Interaction between M. incognita and F. solani as caused of leaf yellowing disease of pepper was synergistic reaction. IntisariPenyakit kuning lada merupakan salah satu penyakit penting pada lada yang mengakibatkan terjadinya penurunan produksi lada di Indonesia. Penelitian ini bertujuan untuk mengetahui peran Meloidogyne incognita dan Fusarium solani sebagai penyebab penyakit kuning lada. Penelitian yang dilakukan rumah kasa dan laboratorium. Meloidogyne incognita dan Fusarium solani diisolasi dari pertanaman lada di Kalimantan Barat. Penelitian dilakukan dengan menggunakan bibit lada kultivar Natar 1 berumur 5 bulan, dan diinokulasi dengan M. incognita sebanyak 1000 larva stadium 2 dan 50 ml suspensi mikrokonidium F. solani dengan kerapatan 106/ml. Perkembangan gejala penyakit diamati setiap minggu selama 5 bulan, dan pada akhir pengamatan dilakukan penghitungan jumlah puru dan populasi M. incognita. Hasil penelitian menunjukkan bahwa apabila M. incognita menginfeksi akar dan selanjutnya diikuti dengan infeksi oleh F. solani, tingkat keparahan penyakit dan persentase tanaman sakit lebih tinggi dibandingkan dengan infeksi oleh F. solani atau M. incognita secara terpisah. Populasi M. incognita dan jumlah puru akar pada tanaman yang diinokulasi dengan M. incognita bersama-sama dengan F. solani lebih tinggi dibandingkan pada tanaman yang diinokulasi dengan M. incognita atau F. solani secara terpisah. Interaksi antara M. incognita dan F. solani dalam menyebabkan penyakit kuning lada adalah bersifat sinergis.

SECONDARY BIOACTIVE METABOLITE GENE CLUSTERS IDENTIFICATION OF ANTICANDIDA-PRODUCING Streptomyces Sp. GMR22 ISOLATED FROM WANAGAMA FOREST AS REVEALED BY GENOME MINING APPROACH Camelia Herdini; Sofia Mubarika; Bambang Hariwiyanto; Nastiti Wijayanti; Akira Hosoyama; Atsushi Yamazoe; Hideaki Nojiri; Jaka Widada
Indonesian Journal of Pharmacy Vol 28 No 1, 2017
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Streptomyces are a group of Gram-positive bacteria belonging to the Actinobacteria class, which are among the most important bacteria for producing secondary bioactive metabolites such as antibiotics, chemotherapeutics, insecticides and other high-value chemicals. Genome mining of gene clusters that encode the biosynthetic pathways for these metabolites has become a key methodology for novel compound discovery. Recently, we have isolated the Streptomyces sp. GMR22 from Cajuput rhizospheric soil at Wanagama Forest, Indonesia. GMR22 produced secondary metabolite that inhibited Candida albicans with IC50 of 62,5 μg/mL. The objective of this work was to reveal the novel secondary metabolites from GMR22 by genome mining approach. The antiSMASH 3.0 was used to predict gene clusters that encode the biosynthetic pathways of secondary metabolites in the genome of GMR22, and their core chemical structures. The pylogenomic analysis showed that GMR22 was closely related to Streptomyces bingchenggensis BCW1, as well as to the large genome size (9.5-12.7Mbp) groups of Streptomyces. AntiSMASH 3.0 analysis revealed that the genome of Streptomyces sp. GMR22 harbored at least 63 gene clusters that encode the biosynthetic pathways of secondary metabolites. It was the highest number of gene clusters had been observed among the members of Streptomyces groups, with PKS was predicted as the major groups of the identified gene cluster products. The results suggested that GMR22 could be a strong potential candidate of secondary bioactive metabolites source.

Peranan Serasah Terhadap Sumbangan N dan P pada Agroekosistem Kopi RUSDI EVIZAL; TOHARI TOHARI; IRFAN D. PRIJAMBADA; JAKA WIDADA
Agrotrop : Journal on Agriculture Science Vol 2 No 2 (2012)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (62.679 KB)

Litter is the main source of organic matter and nutrient entrance in shade grown coffee agroecosystems.Shade trees provide ecological and economic benefits in coffee agro-ecosystems thatindicate the key factor for sustainable coffee production. This study examined the role of litter onnutrient (N and P) returning in coffee agro-ecosystems. The experimental plots of Coffea canephorawere constucted at benchmark site of Conservation and Sustainable Management of Below-GroundBiodiversity (CSM-BGBD), in Sumberjaya Subdistrict, West Lampung, Indonesia, during 2007-2010.Types of shade trees to be examined were legume shade trees of Gliricidae sepium and Erythrinasububrams, non-legume tree of Michelia champaca, and no shade tree. The results showed that: (1)kind of shading determined litter productivity of coffee agro-ecosystems. Litter productivity of coffeeagro-ecosystems with shade trees increased coffee bean yield, (2) litter was an important source of Nand P that returned into the soil. Amount of nitrogen from litter biomass has linier effect on coffee beanyield.

MEKANISME ANTIBIOSIS BACILLUS SUBTILIS B315 UNTUK PENGENDALIAN PENYAKIT LAYU BAKTERI KENTANG Nur Prihatiningsih; Triwidodo Arwiyanto; Bambang Hadisutrisno; Jaka Widada
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 15 No. 1 (2015): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Antibiosis mechanism of Bacillus subtilis B315 for controlling potato bacterial wilt disease. Bacillus subtilis B315 isolated from rhizospheric potato has antibiosis mechanism against Ralstonia solanacearum in vitro and become potentially used as controlling method of bacterial wilt in the field. The objectives of this research were to study the mechanism of B.subtilis B315 in controlling bacterial wilt disease, to study of B. subtilis B315 potency as both biocontrol and plant growth promoter, and to evaluate the mechanism as biocontrol agent. This green house experiment used CRD (Completely Randomized Design) with 5 treatments and 6 replicates. The treatments were control (without B. subtilis B315), B. subtilis B315 wild type, antibiosis mutant M16, antibiosis mutant M4, and antibiosis mutant M14. Variables observed were incubation period, disease index, infection rate, effectiveness of control, and growth components (i.e number of bud, plant height, leaf area, plant fresh and dry weight). The result of this research showed that B. subtilis B315 could delay incubation period, suppressed the disease index up to 64,9% and could promote the plant growth (leaf area). B. subtilis B315 had the antibiosis and other mechanisms that induced sistemic resistance. The implication of this research was that B. subtilis B315 could be used for biocontrol the bacterial wilt and promoted the potato growth.

Biosulfo Fertilizer Development for Horticulture Crops II. The Effect of Phosphate Rock Content and Inoculum Ratio of Biosulfo on P and S Uptake and Yield of Red Onion in Acid and Alkaline Soils Sudadi Sudadi; Sumarno Sumarno; Jaka Widada
AGRIVITA Journal of Agricultural Science Vol 33, No 3 (2011)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v33i3.83

Research aimed to study the influence of phosphate rock-sulfur (PRS) content and inoculum ratios of biosulfo on P and S uptake and red onion yield on acid (Alfisol) and alkaline (Vertisol) soils. Two factors evaluated were PRS content (0%, 60%, 80%) and inoculums of A.niger/P.nalgiovensis ratio of biosulfo (0:0, 1:1 and 3:1). As much of 12 kg of soil (Ø 2 mm) mixed thoroughly with biosulfo, basic fertilizer and manure, put into polybag then watering at field capacity moisture content. One bulb of red onion was planted to each polybag and incubated in the green house. The experiments arranged in completely randomized design with three replications. Variables observed included P and S uptake, and onion yield. Data analyzed with F test at 5% level of significant followed with DMRT if any significant influences. The result shows that the increases of PRS content as well as A. niger ratio of biosulfo tend to increase P and S uptake, especially on acid Alfisol. Highest P and S uptake and onion yield were achieved with treatment combinations of P80I11, P80I31, and P60 I11 for Alfisol, and P80 I11, P60 I11 and P60 I11 for Vertisol respectively. Keywords: phosphate, rock content, inoculums ratio,P and S uptake, onion yield

KARAKTERISASI BAKTERI PENDEGRADASI FENOL DAN PEMBENTUK BIOFILM DARI SUMBER ALAMI DAN ARTIFISIAL Arifah Khusnuryani; Erni Martani; Tri Wibawa; Jaka Widada
Kaunia: Integration and Interconnection Islam and Science Vol. 11 No. 1 (2015)
Publisher : Fakultas Sains dan Teknologi UIN Sunan Kalijaga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/kaunia.1080

Fenol merupakan salah satu polutan air tanah yang memiliki sifat toksik bagi manusia maupun lingkungan. Salah satu teknologi yang dapat diaplikasikan untuk pengolahan limbah fenol adalah melalui bioremediasi dengan memanfaatkan aktivitas bakteri. Secara umum, bakteri di alam akan tumbuh dan membentuk biofilm. Bakteri pembentuk biofilm memiliki beberapa kelebihan, diantaranya mampu bertahan hidup dalam lingkungan yang kurang menguntungkan serta meningkatkan degradasi senyawa rekalsitran, karena bakteri akan saling berinteraksi dan saling melengkapi proses metabolik yang ada. Penelitian ini ditujukan untuk karakterisasi dan identifikasi isolat bakteri pendegradasi fenol dan pembentuk biofilm yang diperoleh dari limbah cair rumah sakit dan industri tekstil serta dari tanah gambut. Karakter yang diamati meliputi karakter morfologi koloni dan sel, serta karakter biokimiawi. Hasil karakterisasi selanjutnya digunakan untuk identifikasi menggunakan analisis profile matching isolat terpilih berdasarkan Bergey’s Manual of Determinative Bacteriology. Hasil menunjukkan bahwa bakteri yang diperoleh dari limbah cair rumah sakit memiliki karakter yang mirip dengan Genus Micrococcus (isolat DL120), Genus Enterobacter (DOK135), dan Genus Bacillus (ATA6). Isolat TU3 dari limbah cair industri tekstil mirip dengan Genus Flavobacterium. Isolat HG1, SG3, dan HP3 yang diperoleh dari tanah gambut berturut-turut menunjukkan karakter yang mirip dengan Genus Alcaligenes, Arthrobacter, dan Rhodococcus.

Co-Authors , Tamad . Tohari A. Alimuddin Achmad Dinoto Adi Laksono Ahmad Romdhon Akira Hosoyama Akira Hosoyama Ali Ikhwan Alim Isnansetyo Amekan, Yumechris Angga Prasetya Anna Safarrida Anna Safarrida, Anna Annisa Yusuf, Wahida Ariani Hatmanti Arif Muliawan Arifah Khusnuryani Arifah Khusnuryani Ariyanti, Nur Fitriana Asrul Asrul Asrul Asrul Atsushi Yamazoe Atsushi Yamazoe Aziz Purwantoro Azwar Maas Bambang Hadisutrisno Bambang HADISUTRISNO Bambang Hadisutrisno Bambang Hadisutrisno Bambang Hariwiyanto Bambang Hariwiyanto Bambang Hendro Sunarminto Bambang Hendro sunarminto Bostang Radjagukguk Camelia Herdini Christanti Sumardiyono Denny Irawati DEWI SESWITA ZILDA Dewi Seswita Zilda Dhani Suryawan Dhani Suryawan, Dhani Diani Mentari Diannastiti, Fani Aulia Didik Indradewa Didik Indradewa Didik Indradewa Dinar Mindrati Fardhani Dionysius Andang Arif Wibawa Dionysius Andang Arif Wibawa, Dionysius Andang Dody Kastono Dolly Iriani Damarjaya Dolly Iriani Damarjaya, Dolly Iriani Donny Widianto Donny Widianto Donny Widianto Donny Widianto Dyah Weny Respatie Edy Meiyanto Eka Tarwaca Susila Putra Eko Hanudin Eko Hanudin EKO IRIANTO Ema Damayanti Ema Damayanti Endang Semiarti Endang Sutriswati Rahayu ENI HARMAYANI Eni Harmayani Erni Martani Erni Martani Eti Nurwening Sholikhah Fatturahman Ridwan, Nur Febriansah , Rifki Galang Indra Jaya Ganis Lukmandaru GINTUNG PATANTIS Gintung Patantis Gintung Patantis Hadi, Ismanurrahman Hari Eko Irianto Hartono Hartono H, Hartono Hera Nirwati Hideaki Nojiri Hideaki Nojiri Indun Dewi Puspita IRFAN D. PRIJAMBADA Irfan Dwidya Prijambada Irwan Suluk Padang Jauhari Syamsiyah Joko Sulistyo Kana Ninomiya Keishi Senoo Keishi Senoo, Keishi Khoirun Nisa Lucia Dhiantika Witasari Lucia Dhiantika Witasari, Lucia Dhiantika M. Mustofa Maria Gratias Mariyatun Mariyatun, Mariyatun Masagus Muhammad Prima Putra Masaya Nishiyama Masaya Nishiyama, Masaya Ma’as, Azwar Melki Melki Mirtani Naima Mohamad Aji Ikhrami Muhammad Dylan Lawrie Muhammad Nur Cahyanto Muhammad Saifur Rohman Mujiyo Mujiyo Mukhlissul Faatih Mukhlissul Faatih1, Mukhlissul Mulyadi Mulyadi Mulyadi Mulyadi Mulyono Mulyono Murwantoko . Mustofa M, Mustofa Mustofa Mustofa Mustofa Mustofa N. Ngadiman Naima, Mirtani Nastiti Wijayanti Nastiti Wijayanti Nastiti Wijayanti Ngadiman Ngadiman . Ngadiman ., Ngadiman Ngadiman N, Ngadiman Noviyanto, Amir Nunuk Supriyatno Nur Edy Nur Prihatiningsih Nurfiani, Sri Ocky Karna Radjasa Oedjijono Oedjijono, Oedjijono Pintaka Kusumaningtyas Prijambada, Irfan Dwidja PUSPITA LISDIYANTI Putra, Sukmana Siswandana Putu Sudira R. Riyanti Rahayu, Endang Sutriswati Reni Nurjasmi Reni Nurjasmi, Reni Riska Wulansari Ristiarini, Susana Riyanti Riyanti Rusdi Evizal Saipul Abbas Sarto SATRIYAS ILYAS Shigeto Otsuka Shigeto Otsuka, Shigeto Shinta Hartanto Shogo Matsumoto Sigit Sunarta, Sigit Siti Kabirun Siti Kabirun SITI KABIRUN Siti Subandiyah Sofia Mubarika Haryana Sri Hastuti Sri Nopitasari Sri Nuryani Hidayah Utami Sri Nuryani Hidayah Utami Sri Nuryani Hidayah Utami Sri Suryanti Sri Suryanti Sri Wedhastri Stalis Norma Ethica Subagus Wahyuono Sudadi Sudadi Suhartiningsih Dwi Nurcahyanti Sumarno Sumarno Sumarno Sumarno Suryanti Suryanti Suryanti Suryanti Susila Herlambang Tohari Tohari TOHARI TOHARI Tomy Listyanto Tomy Listyanto Tri Harjaka Tri Joko Raharjo Tri Rini Nuringtyas Tri Wibawa Tri Wibawa Triwibowo Yuwono Triwidodo Arwiyanto Wangi, Dyah Sekar A P WIDYA ASMARA Widya Asmara Wulansari, Riska Yani Lestari Nuraini Yani Lestari Nuraini, Yani Lestari Yasushi Yoshioka Yenny Sariasih Yose Rizal Yuli Setiawati Yuliana Prahastiwi Yuliana Yuliana Prahastiwi, Yuliana YUSRO NURI FAWZYA Yusro Nuri Fawzya Yusro Nuri Fawzya Yuuki Asano Yuyun Farida Yuyun Farida, Yuyun Zilda, Dewi Zeswita

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