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All Journal Jurnal Medik Veteriner Vitek : Bidang Kedokteran Hewan JURNAL KAJIAN VETERINER Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Parasite Science Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary Kontribusi: Jurnal Penelitian dan Pengabdian Kepada Masyarakat Current Biomedicine Jurnal Agro Veteriner
Yulianna Puspitasari
Divisi Mikrobiologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlannga, Surabaya, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Education Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Mulberry (Morus alba L.) leaf extract enhanced spermatozoa motility, viability, and plasma membrane integrity of rats (Rattus norvegicus) exposed to e-cigarette smoke Ramadhani, Fifi Fauziah; Yulianna Puspitasari; Hermin Ratnani; Budi Utomo; Rochmah Kurnijasanti; Kadek Rachmawati
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 2 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i2.2024.120-128

Abstract

This study investigates the impact of mulberry leaf extract on the viability, plasma membrane integrity, and motility of spermatozoa from male white rats (Rattus norvegicus) exposed to e-cigarette smoke. A total of twenty-five male rats were divided into five groups: negative control (NC), positive control (PC), T1, T2, and T3. All groups, except for the NC group, were exposed to e-cigarette smoke. Rats in the T1, T2, and T3 groups received mulberry leaf extract in doses of 100, 200, and 400 mg/kg bw, respectively, while the NC and PC groups were given a placebo of 1% Na-CMC. Both the mulberry leaf extract and the placebo were administered daily, beginning three days prior to the start of e-cigarette smoke exposure, which lasted for 28 days. Results showed that spermatozoa motility, plasma membrane integrity, and viability in the experimental groups were significantly lower than those in the NC group (p <0.05). Conversely, rats in the T1, T2, and T3 groups that received mulberry leaf extract demonstrated significantly greater spermatozoa viability, plasma membrane integrity, and motility compared to the PC group (p <0.05). The T3 group exhibited the most pronounced improvements, with significantly enhanced spermatozoa viability, membrane integrity, and motility (p <0.05) relative to the PC group. These results indicate that mulberry (Morus alba L.) leaf extract enhanced spermatozoa viability, plasma membrane integrity, and motility in white rats (Rattus norvegicus) subjected to e-cigarette smoke.
Risk factors of pyometra in cat at several clinics in Kediri, East Java, Indonesia Rosyta, Pegy; Rimayanti Rimayanti; Budi Utomo; Jola Rahmahani; Yulianna Puspitasari; Wiwik Misaco Yuniarti
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 2 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i2.2024.115-119

Abstract

Pyometra is a reproductive disease that most often affects cats which is caused by bacterial infection of the uterus. This study aims to determine the risk factors that could influence the occurrence of pyometra in cats. The study was conducted at the Four Leg Pet Care, Asa Animal Clinic, and K-5 Animal Care in Kediri regency in December 2021 to February 2022. This retrospective cohort study took secondary data for the period 2020 to 2021 as research material and found 35 cats diagnosed with pyometra. The odds ratio was calculated to analyze the associations to some factors including age, body weight, use of hormonal contraception, frequency of administration of hormonal contraception, hormonal contraceptive drugs used, dose of hormonal contraception given.  Analysis of the relationship between risk factors for pyometra showed that the factors age, use of hormonal contraception, and frequency of giving hormonal contraceptives each have p values ​​of 2.17, 2.13, and 2.13, respectively. It could be concluded that age, use of hormonal contraception, and frequency of administration of hormonal contraception influenced or had an association with the risk of pyometra in cats.
Morphometric and Molecular Identification of Eimeria Bovis and Eimeria Zuernii on Beef Cattle in Lamongan, East Java, Indonesia Kurniawan, Muhammad 'Ahdi; Suwanti, Lucia Tri; Mufasirin, Mufasirin; Suprihati, Endang; Hastutiek, Poedji; Kusnoto, Kusnoto; Ansori, Arif Nur Muhammad; Puspitasari, Yulianna; Khairullah, Aswin Rafif; Moses, Ikechukwu Benjamin; Pratama, Bima Putra; Riwu, Katty Hendriana Priscilia
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.153-166

Abstract

Lamongan Regency, located in East Java, Indonesia, is a significant center for beef cattle production. Despite its prominence, studies on the identification and differentiation of Eimeria spp. parasites in this region are notably lacking. This research aims to address this gap by evaluating the prevalence of Eimeria spp. and identifying two pathogenic species: Eimeria bovis and Eimeria zuernii. The study involved the collection of 250 fecal samples from beef cattle raised on smallholder farms across Lamongan. Sampling was conducted during the rainy season to optimize the detection of Eimeria infections. Using the sugar flotation method, Eimeria oocysts were isolated from 5–10 g of fecal matter per sample. Molecular identification employed polymerase chain reaction (PCR) assays targeting ribosomal RNA’s internal transcribed spacer 1 (ITS-1) region to detect the pathogenic species. Fecal examination using the Whitlock test revealed a prevalence of Eimeria spp. at 44.45% (111/250). PCR analysis further identified E. bovis with a 238 bp amplicon in Solokuro District and E. zuernii with a 344 bp amplicon in Tikung District, highlighting the presence of these two pathogenic species in distinct geographic areas. The findings underline the need for further research that includes expanded sampling from different regions and cattle breeds and the application of varied diagnostic methods. Such studies will contribute to a more comprehensive understanding of the genetic diversity, distribution, and epidemiology of E. bovis and E. zuernii in Indonesia, supporting improved cattle health and management practices in the region.
Detection of Salmonella sp. in broiler chickens in closed houses using the polymerase chain reaction method Larasati, Anissa Dhea; Sabdoningrum, Emy Koestanti; Nidom, Chairul Anwar; Hidanah, Sri; Rachmawati, Kadek; Puspitasari, Yulianna
Jurnal Agro Veteriner Vol. 9 No. 1 (2025): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v9i1.78646

Abstract

This study aimed to detect the presence of Salmonella sp. in broiler chickens raised in closed house systems using the Polymerase Chain Reaction (PCR) method. Liver and intestinal samples were collected from chickens showing clinical signs such as diarrhea, anorexia, and lethargy, along with pathological lesions observed during necropsy. Bacteriological identification involved isolation using selective media, Gram staining and biochemical tests. Two samples (3A and 3B) showed colony morphology and biochemical characteristics consistent with Salmonella sp. Confirmation using PCR targeting the invA gene (primers 139 and 141, expected amplicon size 284 bp) yielded negative results in both samples, while the positive control successfully amplified the target. The inconsistency between bacteriological and molecular results may be attributed to several factors: absence or mutation of the invA gene in the tested isolates, primer mismatch with local Salmonella strains, or inadequate DNA quality and concentration. These findings suggest that reliance on a single molecular marker may be insufficient for accurate detection and underscore the importance of optimizing PCR conditions. The study highlights the need for locally adapted primers and complementary diagnostic approaches to improve the reliability of Salmonella sp. detection in poultry, particularly in intensive production systems like closed house environments.
The Implementation of Hazard Analysis and Critical Control Point (HACCP) in The Frozen Chicken Meat Distribution Chain in Sikka Regency Dua Bura, Maria Antonia Yersi; Effendi, Mustofa Helmi; Puspitasari, Yulianna; Rahardjo, Dadik; Tyasningsih, Wiwiek; Rachmawati, Kadek
VITEK : Bidang Kedokteran Hewan Vol. 14 No. 2 (2024): VITEK-Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v14i2.289

Abstract

Food safety has become a global issue. Hazard analysis and critical control points (HACCP) is a food safety assurance system in the world that emphasizes prevention efforts. This research is a qualitative descriptive epidemiological study which aims to examine the application of HACCP in the distribution chain of frozen chicken meat in Sikka Regency on the growth parameters of total bacteria, Salmonella, and antibiotic residues through field observations at supplier companies and retailers, as well as laboratory tests on 30 frozen chicken meat samples, and 30 fresh chicken meat samples as a comparison. Laboratory tests found antibiotic residues in 60% of samples (16.7% in fresh chicken meat), and microbial contamination in 33% of samples without Salmonella contamination (40% in fresh chicken meat without Salmonella contamination). Monitoring of antibiotic residues has not been carried out properly by supplier companies because the number of samples is very small, sales in markets without refrigeration facilities for up to five hours together with fresh chicken or near fresh fish allows contamination of chicken meat. HACCP principles have not been properly applied in the frozen chicken meat distribution chain. Guidance and regular monitoring are needed so that the quality of frozen chicken meat is maintained.
CALIFORNIA MASTITIS TEST AND IDENTIFICATION OF ANTIBIOTIC-RESISTANT BACTERIA ON DAIRY CATTLE IN "KUD” SEMEN GANDUSARI SUBDISTRICT, BLITAR RAHMAHANI, JOLA; Puspitasari, Yulianna; Suwarno
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 8 No. 1 (2024): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v8i1.2024.096-105

Abstract

Mastitis is an inflammation of the udder that often affects dairy cows in the production phase. The impact of mastitis losses is quite large, including a drastic decrease in milk quantity and quality. Mastitis can be caused by antibiotic-resistant bacteria in dairy cows, including Escherichia coli, Staphylococcus spp or Streptococcus spp. The results of a recent study stated that the prevalence of mastitis in Indonesia reached 83%. The Coliform Mastitis Test (CMT), used worldwide for early detection of mastitis, has a specificity of 80.6% and a sensitivity of 82.4%. Semen Village Unit Cooperative (KUD) is a cooperative that is the center of milk production in Blitar District, which accommodates the milk of dairy cows from smallholder farms in Blitar District. The high incidence of mastitis caused by the presence of bacteria resistant to antibiotics encourages counseling on mastitis and identifying bacteria that cause antibiotic resistance. Extension activities are carried out by presenting material about mastitis caused by the presence of bacteria resistant to antibiotics with lecture techniques (PowerPoint slides), videos, questions, and answers, and demonstrating the California Mastitis Test on milk samples as an early detection test, as well as identifying bacteria that cause antibiotic resistance. This series of counseling activities is an effort to reduce the incidence of mastitis through increased knowledge and understanding of the community.
Co-Authors Aan Nurfahrudianto Adi Sofyan Ansori, Muhammad Adiana Mutamsari Witaningrum Afandi, Zainal Agus Muji Santoso Aswin, Ahmad Bea Hana Siswati BUDI UTOMO Budiarto Budiarto Budiarto Bura, Maria Antonia Yersi Dua Dadik Rahardjo, Dadik Delaiah, Diena Diyantoro Diyantoro, Diyantoro Dua Bura, Maria Antonia Yersi Elis Irmayanti Endang Sri Mujiwati Endang Suprihati Ericka Darmawan Guruh Sukma Hanggara Hani Plumeriastuti Helen Susilowati, Helen Hermin Ratnani Heru Budiono Hidanah, Sri Hisyam, Mirza Atikah Madarina Jola Rahmahani Jola Rahmahani Kadek Rachmawati Kadek Rachmawati Khairullah, Aswin Rafif Khalissa Farah Alifia Kinasih, Kurnia Nisa Kirana, Sabila Citra Kuncorojakti, Suryo Kurniawan, Muhammad 'Ahdi Kusnoto Kusnoto, Kusnoto Larasati, Anissa Dhea Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Maulana, Firdausy Kurnia Mohamad Amin Moses, Ikechukwu Benjamin Mufasirin Mustika, Yolla Rona Mustofa Helmi Effendi Nidom, Chairul Anwar Permatasari, Dian Ayu Poedji Hastutiek Poppy Rahmatika Primandiri Pratama, Bima Putra Rahadyan, Raihan Alif Raharjo, Hartanto Mulyo Rahmahani, Jola Rahmi Sugihartuti, Rahmi Ramadhani, Fifi Fauziah Rimayanti Rimayanti Riwu, Katty Hendriana Priscilia Rizhal Hendi Ristanto Rochmah Kurnijasanti Rodprasert, Watchareewan Rosyta, Pegy Sabdoningrum, Emy Koestanti Saraswati, Afif Tasya Setiawan Koesdarto Setyowidodo, Irwan Shafina Hanum, Farah Siti Aizah Sri Chusniati Suwarno Suwarno Suwarno Tacharina, Martia Rani Tita Damayanti Lestari Tri Julianto Tyasningsih, Wiwiek Widjiati Widjiati, Widjiati Wijaya, Andi Yasmin Wiwiek Tyasningsih Wiwik Misaco Yuniarti Yeni Dhamayanti