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CRISPR-Cas9 Genome Editing Technology for Zoonotic Disease Control in Indonesia: A Comprehensive Review Adnyana, I Made Dwi Mertha; Eljatin, Dwinka Syafira; Sudaryati, Ni Luh Gede; Adiwinoto, Ronald Pratama; Raza, Syed Meesam; da Cruz , Zito Viegas
Journal of Medicine and Health Technology Vol. 1 No. 1 (2024)
Publisher : Direktorat Riset dan Pengabdian Kepada Masyarakat, Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j30466865.v1i1.1172

Abstract

The increasing emergence of zoonotic diseases originating from vectors, rodents, mammals, and others has increased the potential for outbreaks of pandemics in the Indonesian territory. Although control and prevention have been implemented, these efforts have not yet revealed a bright spot; therefore, elaboration with advanced CRISPR-Cas9 technology is a way to accelerate efforts to control zoonotic diseases in Indonesia. However, there is limited literature on this topic. This review aims to comprehensively describe, identify, and summarize the application of CRISPR-Cas9 genome-editing technology in zoonotic disease control in Indonesia. Our findings show that CRISPR-Cas9 genome editing technology offers an innovative approach for zoonotic disease control by targeting disease vectors, modifying animal reservoirs, improving disease surveillance, enhancing vaccine development, and exploring traditional medicine candidates and immunotherapy. The high level of precision, efficiency, and versatility in targeting genomes capable of disrupting, damaging, and disrupting the disease transmission cycle in pathogens makes CRISPR-Cas9 highly effective. However, challenges such as off-target impacts, regulatory complexity, and ethical considerations must be overcome with inter- and multidisciplinary collaboration to promote transparency, equity, and public engagement throughout the process of implementing this technology in the field, especially in Indonesia.
From silos to systems: Reimagining zoonotic neglected tropical disease management through the lens of one health Adiwinoto, Ronald Pratama; Adnyana, I Made Dwi Mertha; Soedarsono, Soedarsono; Gustam, Tasalina Yohana Parameswari
Svāsthya: Trends in General Medicine and Public Health Vol. 1 No. 3 (2024): November 2024
Publisher : PT. Mega Science Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70347/svsthya.v1i3.61

Abstract

Zoonotic neglected tropical diseases (zNTDs) pose significant global public health challenges, particularly in low-income and middle-income countries. Traditional siloed approaches to zNTD management have proven inadequate for addressing the complex interplay between human, animal, and environmental health factors. This review examined the limitations of fragmented strategies and explored the potential of integrated one-health approaches for more effective zNTD control. This narrative review covered publications from 2000 to 2023, sourced from databases including PubMed and Scopus, emphasizing studies that met inclusion criteria of peer-reviewed research focused on zNTDs through a One Health lens. A comprehensive analysis of the current literature and case studies was conducted to evaluate the multifaceted drivers of zoonotic pathogen spillover, assess the economic and health impacts of zNTDs, and identify successful implementation of the One Health strategy in endemic regions. This review highlights the inadequacies of siloed disease management approaches, which often lead to inefficient resource allocation and missed opportunities for synergistic intervention. Successful case studies have demonstrated the effectiveness of the One Health strategy in improving disease surveillance, enhancing cross-sectoral collaboration, and achieving sustainable health outcomes. The potential of emerging technologies and innovations to support integrated approaches has also been explored. Transitioning from siloed to systemic approaches in zNTD management requires overcoming institutional barriers, fostering cross-sector collaboration, and implementing multitiered frameworks that operate at the local, national, and global levels. Investing in cross-sectoral capacity development and leveraging technological innovations are crucial for building a workforce capable of addressing the complex challenges posed by zNTDs. Adopting the One Health principle offers a promising path toward more comprehensive, efficient, and sustainable strategies for zNTD prevention.
Raising the flag of vigilance on Mpox outbreak in Indonesia: are we prepared? Adnyana, I Made Dwi Mertha; Maulana, Sidik; Lutfian, Lutfian; Adiwinoto, Ronald Pratama
Svāsthya: Trends in General Medicine and Public Health Vol. 1 No. 2 (2024): September 2024
Publisher : PT. Mega Science Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70347/svsthya.v1i2.63

Abstract

In the wake of the COVID-19 pandemic, the world has become acutely aware of the devastating impact of infectious diseases on public health, economies, and societies. As we gradually recover from the aftermath of COVID-19, another potential threat looms on the horizon, mpox, formerly known as monkeypox. The recent surge in mpox cases worldwide has prompted health authorities to sound an alarm, and Indonesia, as a populous nation with a complex healthcare system, must ask itself: Are we prepared for this emerging challenge?. The question "Are we prepared?" may not have a simple yes or no answer. Instead, it should serve as a reminder of the need for vigilance, adaptation, and continuous improvement in public health systems. As we navigate this new health challenge, the true measure of Indonesia's preparedness is its ability to respond swiftly, effectively, and equitably to protect the health and well-being of all citizens.
KARAKTERISTIK RHINOSINUSITIS KRONIS DI POLI THT RSPAL dr. RAMELAN SURABAYA PERIODE JANUARI 2020- JULI 2022 Chendikia Abrian Putra Perdana; Chonifa Wahyurini; Ronald Pratama Adiwinoto
Surabaya Biomedical Journal Vol. 5 No. 3 (2026): May
Publisher : Fakultas Kedokteran, Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/v5i3.193

Abstract

Rhinosinusitis adalah suatu keadaan dimana terjadinya infeksi dan peradangan pada sinus mukosa paranasal, dimana setidaknya terjadi 12 minggu. Rhinosinusitis sendiri, merupakan salah satu masalah kesehatan dengan prevalensi yang besar di dunia. Penelitian ini dilakukan untuk mengetahui karakteristik pada pasien rhinosinusitis kronis yang ada di poli THT RSPAL dr. Ramelan Surabaya Periode Januari 2020 – Juli 2022. Penelitian deskriptrif ini dilakukan dengan desain cross-sectional dengan metode kuantitatif. Pengambilan data menggunakan data sekunder rekam medis dan kemudian dilakukan pencatatan untuk mengetahui karakteristik pada pasien. Pemilihan sampel menggunakan metode purposive sampling. Hasil penelitian yang dilakukan pada 30 pasien didapatkan jenis kelamin terbenyak adalah perempuan dengan jumlah 23 pasien (76,66%), kelompok usia terbanyak adalah 41-50 tahun dengan jumlah 11 pasien (36,7%), gejala terbanyak adalah hidung tersumbat dengan jumlah 24 pasien (80%), hasil pemeriksaan terbanyak adalah konka hiperemi dan udem dengan jumlah 23 pasien (76,66%), dan untuk tindakan manajemen terbanyak adalah medikamentosa dan tindakan operatif dengan jumlah 16 pasien (53,33%).
Breaking the Delay: Stage III HIV Diagnosis and Successful Recovery at the Primary Healthcare Level Sahika Nisandi Ayudelvi; Ronald Pratama Adiwinoto; Diadema Dicky Fansia; Muzna Anisah; Khakan Baguna; Iriyadi Iriyadi; Eltari Primasari
Qanun Medika - Jurnal Kedokteran FK UMSurabaya Vol 10 No 02 (2026): Qanun Medika Vol 10 No 02 July 2026
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jqm.v10i02.30290

Abstract

Human Immunodeficiency Virus (HIV) remains a major public health problem, particularly when diagnosis occurs at an advanced stage. This case report describes a 24-year-old male diagnosed with WHO stage III HIV infection at a primary health care facility in the absence of CD4 count and baseline viral load due to resource limitations. The patient presented with oral candidiasis, chronic diarrhea, prolonged fever, and significant weight loss, alongside high-risk sexual behavior. HIV and syphilis serology were reactive, while tuberculosis was excluded despite strong clinical suspicion. The patient was managed with antifungal therapy, syphilis treatment, and first-line antiretroviral therapy (ART). Significant clinical improvement was observed within one month, and viral load decreased to 60 copies/mL at six months, indicating near-complete virological suppression. This case highlights missed opportunities for early HIV diagnosis, supports the use of WHO clinical staging as a surrogate in resource-limited settings, and demonstrates that effective HIV management can be achieved at the primary care level.
The clinical value of systemic immune-inflammation index (SII) and prognostic nutritional index (PNI) for predicting the occurrence of metastasis in patients with lung cancer Verna Biutifasari; Ellen Angelique Hardjono; Ronald Pratama Adiwinoto; Janto Poernomo Hadi
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 1 (2025): Advances in Biomarkers, Therapeutics, and Probiotics: Recent Updates in Medical L
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i1.5628

Abstract

Systemic immune-inflammation index (SII) and prognostic nutritional index (PNI) serve as simple and practical tests that help indicate inflammatory and nutritional status to some extent. Lung cancer stands out as the most common contributor to cancer-related mortality globally. It is associated with an unfavorable prognosis. Most patients diagnosed with lung cancer have metastasis at the time of diagnosis. Prognostic heterogeneity of cancer patients causes the need for more prognostic biomarkers. This study aimed to evaluate the clinical value of SII and PNI in predicting metastasis in lung cancer patients. SII and PNI provides a prognostic value in lung cancer. Retrospective cross-sectional research was conducted in this study involving 138 data from medical records at the Inpatient and Outpatient Department of Pulmonology, RSPAL dr. Ramelan Surabaya in April 2019 to July 2023. Kolmogorov-Smirnov test, contingency coefficient test, and ROC analysis were done to analyze the data obtained. Patients with metastatic lung cancer had higher SII than those without metastasis. The group of patients with metastasis had an average SII of 5391.34 and a PNI of 40.11. The group of patients without metastasis had an average SII of 2849.52 and PNI of 43.05. Lung cancer metastasis was correlated significantly with SII but not with PNI. The cut-off value was determined using the ROC curve. The cut-off value for SII was 2198.54 (68.5% sensitivity and 58.7% specificity) and for PNI was 42.2 (62% sensitivity and 54.3% specificity). SII was correlated with lung cancer metastasis and may be a promising indicator predicting of metastasis. PNI showed no significant correlation with lung cancer metastasis.
Breaking The Cycle: Maternal Knowledge, Sanitation, dan Diarrhea Prevention in Toddlers widya fadhilascha; Danny Wresniwira Danandjaja; Ronald Pratama Adiwinoto; Retno Wisanti
CoMPHI Journal: Community Medicine and Public Health of Indonesia Journal Vol. 6 No. 3 (2026): Februari
Publisher : Perhimpunan Dokter Kedokteran Komunitas dan Kesehatan Masyarakat Indonesia (PDK3MI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37148/comphijournal.v6i3.326

Abstract

This research aims to explore the relationship between maternal knowledge and sanitation hygiene with the incidence of diarrhea in young children within the Socah Community Health Center's service area. The study employs a quantitative approach with an observational cross-sectional design. Primary data was gathered using a questionnaire assessing maternal knowledge and daily sanitation hygiene practices. Data analysis was conducted using both univariate and bivariate methods, including the chi-square test. The findings reveal that, among 101 mothers with toddlers who met the inclusion criteria, most had low knowledge (40.6%) and moderately implemented hygiene and sanitation practices (37.6%). Additionally, 47.5% of the children had experienced diarrhea in the past two weeks. The chi-square test showed a significant correlation between maternal knowledge and the occurrence of diarrhea (p < 0.001), as well as between hygiene and sanitation practices and the incidence of diarrhea in children (p < 0.001). The study concludes that both maternal knowledge and sanitation hygiene significantly affect the occurrence of diarrhea in children in the Socah Community Health Center area. It is recommended that health education regarding diarrhea prevention, personal hygiene, and environmental sanitation be enhanced to reduce diarrhea rates among infants. This research is intended to serve as a foundation for the planning of health support and prevention programs in the Community Health Center's service area.
CLINICAL FEATURES IN NEPHROTIC SYNDROME PATIENTS: A CASE REPORT Made Maylisca Theresia Mulya Diprasta; Lavenda Diarnasa Zihara Safira; Levina Larassanti; M. Shadiqul Amin; Ronald Pratama Adiwinoto
Jurnal Impresi Indonesia Vol. 3 No. 1 (2024): Jurnal Impresi Indonesia
Publisher : Riviera Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58344/jii.v3i1.4438

Abstract

Nephrotic Syndrome is a glomerular disease characterized by edema, massive proteinuria >3.5 grams/day, hypoalbuminemia <3.5 grams/day, hypercholesterolemia, and lipiduria. Nephrotic syndrome has a variety of metabolic effects that impact the individual. Some episodes of nephrotic syndrome are self-limited, and some respond to specific therapy, while others are chronic. A 49 year old female patient complained of frequent sweats at night and chills in the morning. Then, the patient complained that his legs had been swollen since he left the hospital until now. Apart from that, edema also occurs in the lower extremities. The management given is general management such as rest, low cholesterol, low salt, fluid restriction <1500ml, and a protein diet. Treatment of edema with diuretics, treatment of proteinuria, treatment of hypertension and history of stroke, as well as causal treatment according to the etiology of nephrotic syndrome. The patient has undergone management planning and treatment therapy for nephrotic syndrome.
The Relationship Between Feeding Patterns and Maternal Knowledge about Nutrition with the Incidence of Stunting in Children Age 0-5 Years in the Working Area of the Gedongan Health Center, Mojokerto City Nadya Aisyah Sasqia Putri; Ronald Pratama Adiwinoto; Prima Arundani; Pramita Anindya Nugraheni; I Made Dwi Mertha Adnyana
AgriHealth: Journal of Agri-food, Nutrition and Public Health Vol 5, No 1 (2024): April
Publisher : Research and Development Center for Food, Nutrition and Public Health (P4GKM) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agrihealth.v5i1.83808

Abstract

Stunting is a prolonged state of chronic malnutrition. This disorder is characterized by having a body length-for-age or body height-for-age measurement that falls below the -2 standard deviation established by the World Health Organization (WHO). The etiology of stunting is multifaceted, encompassing factors such as nutrition, feeding practices, infectious illnesses, and household income. Maternal understanding of nutrition, including dietary factors, is also associated with the occurrence of stunting. This study aimed to establish the relationship between feeding habits and maternal knowledge of nutrition with the prevalence of stunting in the operational region of the Gedongan Health Center, located in Mojokerto City. This study is an observational analytical study employing a cross-sectional design. The study’s population consisted of all women with children residing in the working area of the Gedongan Health Center. This study encompassed 144 participants who completed the Child Feeding Questionnaire (CFQ) regarding eating practices and a questionnaire assessing maternal knowledge of nutrition. The objective was to examine the relationship between these factors to height and age. The chi-square test results indicate a statistically significant association between feeding practices and stunting (p = 0.0001) and maternal knowledge about nutrition and stunting (p = 0.0001). The survey revealed that most children had been provided with appropriate feeding patterns, and most moms had a commendable nutritional understanding. The study’s findings indicate a noteworthy relationship between feeding behaviors and mothers’ nutrition knowledge.
Empowering Communities: Harnessing Technology for HIV Literacy and Triple Zero Goals Ronald Pratama Adiwinoto; Jennifer Wijaya; Alicia Stevina Martono; Fernita Naomi Putri; Vincentia Prasasti; Hanung Prasetya
Journal of Health Promotion and Behavior Vol. 9 No. 4 (2024)
Publisher : Masters Program in Public Health, Sebelas Maret University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26911/thejhpb.2024.09.04.03

Abstract

Background: The COVID-19 pandemic provides a unique opportunity to reinvigorate efforts to achieve the Triple Zero HIV goal by 2030-zero new cases, zero deaths from AIDS, and zero stigma. This scoping review explores technology, including social media and big data, in increasing HIV literacy and community engagement. It is crucial to prioritize the Triple Zero HIV targets, which involve the eradication of new HIV infections, AIDS-related deaths, and the stigma associated with HIV, amidst the COVID-19 epidemic. This study aimed to rigorously investigate and identify new technology-driven therapeutics to eliminate HIV, AIDS-related fatalities, and social discrimination by 2030. Subjects and Method: A systematic review was conducted by searching articles from PubMed and ResearchGate is an article published from 2015 to present. The keywords were “HIV education technology” and “community engagement.” Articles covering technology-based HIV literacy and community engagement were selected, and other databases were searched extensively for this scoping review. The search included "HIV education technology” “Community engagement” and "Big data in HIV prevention”. The articles were selected by PRISMA flow diagram method. Results: A total of 8 studies was included in this review. This study revealed innovative approaches to HIV education through technology, including mobile applications and social media campaigns. These tools provide easily accessible information, reducing stigma and discrimination. Big data and predictive analytics can target interventions effectively. The post-COVID-19 era allows for community mobilization through social media campaigns and virtual support groups, fostering solidarity and access to care. According to our analysis, mobile apps and social media campaigns are novel HIV education methods. These tools make knowledge accessible, decreasing stigma and discrimination. Big data and predictive analytics target interventions. Community mobilization through social media campaigns and virtual support groups promotes solidarity and care post-COVID-19. Conclusion: Technology, heightened health awareness, and community engagement can drive progress toward the Triple Zero goal of HIV by 2030, creating a world with no new cases, no AIDS deaths, and no stigma. This opportunity arises in the wake of the COVID-19 pandemic, which offers new avenues for HIV prevention and care.
Co-Authors Adisyahputra, Vega Adnyana , I Made Dwi Mertha Agung Dwi Wahyu Widodo Agung Pranoto Agus Setyobudi Ais, Hasna Rahadatul Al Hasan, Hafidz Ramadhan Alicia Stevina Martono Alpha Fardah Athiyyah Amelya Betsalonia Sir ANNISA ULLYA RASYIDA Ariewibawa, Fariz Atiqi Artha Budi Susila Duarsa Astrid Kusuma Avelline, Kendraadine Dheayu Baita, Etina Noor Betty Agustina Tambunan Brilliandinda Ichsasqia Sidqi Auliayahya Brilliant , Fahridho Ramadhana Cahya Yudha Laksmana Putra Chendikia Abrian Putra Perdana Chonifa Wahyurini da Cruz , Zito Viegas Danny Wresniwira Danandjaja Devanda, Kasilda Pasha Dewi, I Gusti Ayu Ajeng Widia Kencana Diadema Dicky Fansia Diah Utami, Prawesty Diana, Adawiyah Putri Distyvanya, Shakira Azzahra Djatiwidodo Edi Pratiknya Dwinka Syafira Eljatin Dwitama Adiwinoto, Robert E.J.T, SIHNING Effendi, Muchiddin Andri Setyo Eka Fitriani Hafel Ellen Angelique Hardjono Eltari Primasari Erina Yatmasari Ersanda Nurma Febri Endra Budi Setyawan Fernita Naomi Putri Florentina Sustini Graciela, Anne Maria Hanung Prasetya Hardiono Hardiono, Hardiono Hardjono, Ellen Angelique Hartono Kahar, Hartono Hayati, Khofifah Bela Rachmanu Helda Dwiana Sari HENDRATA ERRY ANDISARI2 Herdiyantini, Mita Heru Nurdianto Hibatullah, Faiz Nur Hidajat, Boerhan Iriyadi Iriyadi Irza Hasanal Hamdy Ivana Angelica Haris Izzah Al Nabilah Janto Poernomo Hadi Jaudah Nahdah Jeanaya Hedya Alfara Putri Jenniefer Clarissa Putri Jennifer Wijaya Jongky Hendro Prajitno Kalada, Elisabeth Chelsea Uma Ketut Edy Sudiarta Khakan Baguna Kharizmatika Kresnamurti, Angelica Lauwis, Keziah Queency Lavenda Diarnasa Zihara Safira Levina Larassanti Liliawanti Liza Yudistira Yusan Lutfian, Lutfian M. Shadiqul Amin Made Ayu Mira Cyntia Dewi Made Maylisca Theresia Mulya Diprasta Made Peni Juliana Maliku Nurrochman Widandi Manata Wijaya Sasmita Martono, Alicia Stevina Maulana Muchammad Aunun Najib Maulana, Sidik Medina, Nadra Odifa Meiriyanti Dhita Mentari Nata Kusuma Michael Bramantya Michelle Madeline Maydavania Miranda, Stefani MOCHAMAD ALVIRIO NEDYA RIZKA Muhammad Kalaznykov MUHAMMAD RIZAL Muzna Anisah Nadya Aisyah Sasqia Putri Najmasari, Neyza Farah NANDA ABIGAIL Ni Komang Sri Dewi Untari Ni Luh Gede Sudaryati Norholila Nugraheni, Pramita Anindya Oki Nugraha Putra Olivia Mahardani Adam Peppy Nawangsasi Peter Gunawan Tandean Poerwanto, Eka Pramita Anindya Nugraheni Pramita Anindya Nugraheni Pramudita Riwanti Prasasti, Vincentia Prasetya, Hanung Prasetyadi , Frans O.H. Prima Arundani Purmata, Andrew Theo William Chris Putra, Fabianus Yvan Marino Cahaya Putra, Oki Nugraha Putri, Fernita Naomi Putri, Ida Ayu Govinda Lazenobia De Sanjaya Raharja, Febrila Tarisa Rahmadita, Annisa Putri Raza, Syed Meesam Renata Prameswari Retno Budiarti Retno Budiarti Retno Wisanti Riami Ristanti, Safira Rizqi Rokhmadhoni Pikir Robert Dwitama Adiwinoto Rokhmadhoni Pikir, Rizqi Rr. MUTIARA DEVANIA SAMARA QALBA Rusli, Christina S. Soedarsono Sadya Wendra SAES, JOVITASARI ELVIANA PUTRI Safira, Vita Sahika Nisandi Ayudelvi Sandy, Emy Noviana Santoso, Clara Cindy Ateng Saptono Putro Setyawan, Yuswanto Sianipar, Habel Ryan Annerico Soedarsono Soedarsono Soelistijo, Soebagijo Adi Sri Umijati Stefania Laku Loi Sudiarta, Ketut Edy Sulistiawati Sulistiawati Sulistiawati Sulistiawati Sulistio, Timothy Sunur, Vincentius William Sylvia Rizki Tamam Jauhar Tasalina Yohana Parameswari Gustam Titong Sugihartono Ubaid, Angling Febriani Ariqoh Usman Hadi Utami , Prawesty Diah Vendra Setiawan Verna Biutifasari Vincentia Prasasti Wahyu Prasasti Mutiadesi Wahyu Prasasti Mutiadesi Widati Fatmaningrum Widjajanto , Hendra widya fadhilascha Wijaya, Jennifer Wijaya, Rike Andy Yulia E Nitbani Zeeva, Evane Jovanie Zunnahri, Salsabila Qurrotul Aini