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All Journal Majalah Kedokteran Bandung Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Folia Medica Indonesiana Jurnal Promkes: The Indonesian Journal of Health Promotion and Health Education The Indonesian Biomedical Journal Jurnal Penelitian Kesehatan Suara Forikes Journal Of Vocational Health Studies Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya EMBRIO : Jurnal Kebidanan Jurnal Kreativitas PKM Care : Jurnal Ilmiah Ilmu Kesehatan ISM (Intisari Sains Medis) : Jurnal Kedokteran Biomolecular and Health Science Journal JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Majalah Biomorfologi (Biomorphology Journal) Jurnal Layanan Masyarakat (Journal of Public Service) JOURNAL OF COMMUNITY MEDICINE AND PUBLIC HEALTH RESEARCH Indian Journal of Forensic Medicine & Toxicology Lumbung Inovasi: Jurnal Pengabdian Kepada Masyarakat INDONESIAN MIDWIFERY HEALTH AND SCIENCES JOURNAL Narra J Indonesian Andrology and Biomedical Journal The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy Strada Jurnal Ilmiah Kesehatan (SJIK)

I'tishom, Reny

Departemen Biologi Kedokteran Fakultas Kedokteran Universitas Airlangga

Author-ID : 1452839

Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

Published : 63 Documents Claim Missing Document

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Role of antioxidant to protect Leydig cells induced by reactive oxygen species: a literature review Anak Agung Istri Dalem Cinthya Riris; Reny I'tishom; Siti Khaerunnisa
Qanun Medika - Jurnal Kedokteran FK UMSurabaya Vol 5, No 1 (2021)
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jqm.v5i1.4693

The Leydig cells play crucial role in steroidogenesis and spermatogenesis. Those processes need complex communication in hormonal and testicular to maintain male reproductive function. Abnormal condition induced by reactive oxygen species reduce cell viability through lipid peroxidation and apoptotic pathway. Antioxidant ameliorate ROS elevation and prevent cell damage. Specifically, Leydig cells are vulnerable to ROS exposure and decline its function in mediating spermatogenesis. Therefore, it is needed to improve Leydig cells viability within antioxidant supplementation. This study aimed to determine the protective effect of antioxidant on Leydig cells induced by reactive oxygen species. This type of study is a literature review. Various studies have been reviewed through critical appraisal tool Olsen-Baisch Scoring for integrated review. Furthermore, this study highlighted the importance of the mechanism of antioxidant as protective agent of Leydig cells. Supplementation of antioxidant with the correct administration, dosage, and duration is potential to balance reactive oxygen species level and protect Leydig cells. Keywords : Leydig cell, antioxidant, reactive oxygen species, infertility

Solanum betaceum Extract as a Protective on Spermatozoa Morphology of Mice Exposed to Lead Acetate Rima Wirenviona; Reny I'tishom; Siti Khaerunnisa; Anak Agung Istri Dalem Cinthya Riris; Nurul Fatimah Susanti; Nurul Jannatul Wahidah; Abadiyah Zakiah Kustantina
Qanun Medika - Jurnal Kedokteran FK UMSurabaya Vol 5, No 1 (2021)
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jqm.v5i1.4594

Environmental pollution is one of the factors that contribute to the decline in male fertility. Lead is one of six air pollutants harmful to the reproductive system. One parameter of infertility in men is an increase in reproductive function observed with decreased morphological abnormalities of spermatozoa. The purpose is to analyze the effect of giving various dosages of Solanum betaceum extract on spermatozoa morphology of mice exposed to lead acetate. This study was true experimental using a randomized post-test only control group design. The total sample was 40 male mice Balb/ C taken by simple random sampling technique. Treatment and maintenance of experimental animals for 35 days. Statistical tests showed there were significant differences with p-value 0.005. Solanum betaceum extract can be used as a protective to improve the normal morphology spermatozoa of mice that exposed to lead acetate.

Ethanolic extract of Dutch eggplants (Solanum betaceum) protects spermatozoa motility exposed to lead acetate Rima Wirenviona; Reny I'tishom; Siti Khaerunnisa
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 52, No 4 (2020)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Lead is a toxic material that can have negative effects on reproductive organs. Lead exposure reduces the activity of endogenous antioxidant enzymes and increases the number of oxidants in the body. High free radicals will affect spermatogenesis and result in decreased motile spermatozoa. Antioxidants are known to protect the motility of spermatozoa, and adequate antioxidants can be found in Dutch eggplants (Solanumbetaceum). This study aimed to evaluate the effect of S. betaceum on spermatozoa motility after exposure to lead acetate. This study is a true experimental design with a randomized post-test-only control group design. Forty male Balb/C mice 12 weeks old were randomly divided into 5 groups: two control groups (C0, C1) and three treatment groups (T1, T2, T3).The C0 received distilled water, and the C1 received 75 mg/kg BW lead acetate. The T1, T2 and T3 received 100, 200 and 400 mg/kg BW of S. betaceum, respectively, an hour before exposed lead acetate. The data were analyzed using one-way ANOVA with a significant level of p <0.05. A significantly increasein the mean total motility of spermatozoa in T1, T2, and T3 was reported.This study indicates that S. betaceum have a protective effect on spermatozoa motility when exposed to lead acetate.

Dedifferentiation of MCF-7 Breast Cancer Continuous Cell Line, Development of Breast Cancer Stem Cells (BCSCs) Enriched Culture and Biomarker Analysis Ami Ashariati Prayogo; Andi Yasmin Wijaya; Winona May Hendrata; Steven Sheng Looi; Reny I’tishom; Lukman Hakim; Fedik Abdul Rantam; I Ketut Sudiana; Abdurachman Abdurachman
The Indonesian Biomedical Journal Vol 12, No 2 (2020)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v12i2.977

BACKGROUND: Cancer stem cells (CSCs) eradication might serve as a robust approach for cancer eradication. MCF-7 as breast cancer continuous cell line is known to contain breast CSCs (BCSCs) for its capability to maintain its original tumor population. CSCs enriched culture is a fundamental tool for CSCs targeted therapy development. Effective and unsophisticated CSCs dedifferentiation protocol for producing CSCs enriched culture is needed.METHODS: MCF-7 cells were cultured initially in Dulbecco's Modified Eagle Medium (DMEM) low glucose medium then changed to DMEM:F12. Serum starvation was performed during each medium refreshment gradually with fetal bovine serum (FBS) concentration of 10%, 5%, 2.5% until reaching 1% FBS concentration. Stable MCF-7 culture was then adapted to serum free culture system, containing DMEM:F12, epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), and B27 supplement as dedifferentiation protocol for 18 days. Cluster of differentiation (CD)44 and CD24 double staining immunocytochemistry was performed to evaluate cell stemness.RESULTS: The population of cells expressing BCSCs markers (CD44+/CD24low) in non-adherent single cells subpopulation was significantly increased after the dedifferentiation procedure (70.39%) compared to control groups (0.71%) (p<0.05). In contrast, the expression of BCSCs marker in adherent single cells subpopulation and for both adherent and non-adherent mammosphere the BCSCs markers showed a stable expression.CONCLUSION: BCSCs enrichment of breast cancer cell cultures from MCF-7 breast cancer cell line can be performed. Breast cancer cell plasticity is observed during the dedifferentiation protocol. Development of dedifferentiation inducing protocols can serve as an important foundation for breast cancer therapy development through BCSCs elimination.KEYWORDS: breast neoplasms, cell line, dedifferentiation, immunohistochemistry, neoplastic stem cells

EFFECTS OF RED DRAGON FRUIT (Hylocereus polyrhizus) SKIN EXTRACT ON LEAD ACETATE TOXICITY IN THE MORPHOLOGY OF Balb/c MICE (Mus musculus) SPERMATOZOA Rahmawati Raharjo; Sri Agus Sudjarwo; Reny I'tishom
Folia Medica Indonesiana Vol. 53 No. 4 (2017): December 2017
Publisher : Faculty of Medicine, Universitas Airlangga

Red dragon fruit skin extract is used as an antioxidant to lead acetate toxicity. This study aimed to prove the difference in morphology of mice testicular spermatozoa exposed to lead acetate. Twenty-five mice were divided into 5 groups (5 mice per group). K- group was the control group without lead acetate and dragon fruit skin extract administration, K + group was treated with 8 mg/KgBW lead acetate administration for 14 days, P1 group was treated with the administration of 8 mg/KgBW lead acetate for 14 days + 250 mg/kgBW red dragon fruit skin extract for 21 days, P2 group was treated with the administration of 8 mg/KgBW lead acetate for 14 days + 500 mg/kgBW red dragon fruit skin extract for 21 days, and group P3 was a treatment group with the administration of 8 mg/KgBW lead acetate for 14 days + 1000 mg/kgBW red dragon fruit extract for 21 days. The results showed that there was significant difference (P<0.05) in normal sperm morphology among K-, K +, P1, P2, and P3. In conclusion, the administration of red dragon skin extract at a dose of 500 mg/kgBW (P2) is the optimal one which can be used as therapy to increase motility, morphology, and concentration of mice spermatozoa exposed to lead acetate.

EFFECTS OF LYCOPENE ON SPERMATOZOA MORPHOLOGY IN Balb/C MICE EXPOSED TO 2-METHOXYETHANOL (2-ME) Sandra Dewi Sitaresmi; Reny I'tishom; Arifa Mustika
Folia Medica Indonesiana Vol. 53 No. 4 (2017): December 2017
Publisher : Faculty of Medicine, Universitas Airlangga

The compound of 2-methoxyethanol (2-ME) is one of toxic materials that potentially damage male reproductive organs. This compound can enter the body by various means of direct contact through the skin, respiratory and digestive tract. This compound can cause oxidative stress in spermatozoa which is the main cause of spermatozoa dysfunction. Lycopene, also known as red pigment, is an antioxidant class of carotene. Lycopene has antioxidant activity twice stronger than beta carotene and ten times stronger than vitamin E. Thus, the lycopene reaction as an antioxidant in the body is better than vitamin A, C, E, and other minerals. This study was conducted to determine the effects of lycopene on spermatozoa morphology in mice exposed to 2-ME. The study was conducted on 30 mice, divided into 5 groups. The K-group was the control group without 2-methoxyethanol and lycopene, the K + group was the group with 200 mg/kg BW 2-methoxyethanol on days 1-5, and the P1, P2 and P3 groups were the ones with 200 mg/kg BW 2-methoxyethanol on days 1-5 and lycopene doses of 5, 10 and 20 mg/kg BW on days 6-35. 2-ME was given intraperito-neally and lycopene was given per sonde. On day 36, the mice were sacrificed and the epididymis and vas deferens were removed for morphological examination of spermatozoa. The observations were performed using a microscope with 1000x magnification. The results showed that there was significant difference. In the morphology of spermatozoa, the administration of lycopene increases normal morphological percentage of spermatozoa in mice exposed to 2-ME.

A Systematical Review of The Effect of Ketogenic Diet on Bcl-2 (B-cell lymphoma-2) Expression as An Apoptosis Marker in Cancer Treatment Vania Islamey Kusuma; Reny I’tishom; Ema Qurnianingsih; Purwo Sri Rejeki
Biomolecular and Health Science Journal Vol. 4 No. 2 (2021): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bhsj.v4i2.30173

Introduction: Ketogenic diet seems to be in a great demand nowadays as a lot of people are interested in adopting it into their lifestyle. It is also found that the ketogenic diet shows several beneficial effects including cancer prevention. However, the detail mechanism still remains unknown. Therefore, this review was aimed to find out the effect of ketogenic diet on Bcl-2 (B-cell lymphoma-2) expression in cancer.Methods: We searched published literatures in PubMed through 2011-2020 using specific keywords. The literatures were filtered according to inclusion and exclusion criteria. Animal model, total sample size, underlying condition/inflammatory process occurs, details of the intervention/diet including diet contents in control group and high-fat group, and the duration of the intervention, Bcl-2 results, and p-value were extracted.Results: 7 studies were included in this review. Bcl-2 expression found decrease in 5 out of 6 studies. Similar result also obtained in Bcl-2 protein level, which measured by western blot. Bcl-2 protein level shows a decrease in 2 out of 3 studies.Conclusion: This review shows that high-fat diet that contained in ketogenic diet most likely lead to decrease in Bcl-2 expression. Therefore, indicating the ability of ketogenic diet to affect cancer progression by inducing apoptosis process.

CORRELATION BETWEEN KNOWLEDGE OF CITY OF RESIDENCE AND COVID19 IN SURABAYA YOUTH GENERATION Ricky Indra Alfaray; Lionardy Yodianto; Astri Dewayani; Kartika Afrida Fauzia; Dwiki Noni Armyta; Yudith Annisa Ayu Rezkitha; Reny I'tishom; Muhammad Miftahussurur
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 5 No. 1 (2021): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v5i1.2021.116-122

COVID19 pandemic became the primer focus of health problem by the government since the case was increasing every month in 2020. The impact of this pandemic was spread quickly across many areas including Surabaya city. Youth generation often found to be a silent source of infection. Furthermore, young people behavior often ignoring health prevention policy makes the infection more easily to spread. This condition might be because of their knowledge regarding COVID19 was relatively not enough. Factor that may affect their knowledge are varies including knowledge of city of residence. This study aims to evaluate the correlation between knowledge about city of residence with youth generation knowledge of COVID19 as this factor has not yet been studied before in Indonesia. We conducted an analytical cross-sectional with an online survey for youth generation across Surabaya to understand their knowledge concerning Surabaya and COVID19. A total of 57 participants were voluntarily enrolled in this study. The result data were analyzed statistically. There is significant positive correlation between participants’ knowledge of Surabaya city with knowledge of COVID19 (r= 0.314; p= 0.017). Furthermore, knowledge of COVID19 was significantly different between youth who have better knowledge of Surabaya than lower knowledge (p= 0.021). Knowledge of Surabaya city was correlated with knowledge of COVID19. Youth who have better knowledge regarding their city of residence seem to have better knowledge of COVID19. Knowledge concerning city of residence proven to be important in order to increase knowledge of COVID19 in youth generation.

THE MUTATION STATUS OF KRAS GENE CODON 12 AND 13 IN COLORECTAL ADENOCARCINOMA (Status Mutasi Gen Kras Kodon 12 dan 13 di Adenocarcinoma Kolorektal) Gondo Mastutik; Alphania Rahniayu; Anny Setijo Rahaju; Nila Kurniasari; Reny I’tishom
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 1 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i1.1177

Kanker kolorektum merupakan salah satu kanker yang tersering di dunia. Target molekuler untuk pengobatan kanker kolorektumyaitu Epidermal Growth Factor Receptor (EGFR) dengan pemberian antibodi monoklonal anti-EGFR. Pemberian pengobatan ini tidakdapat memberikan efek dampak di pasien dengan status gen KRAS bentuk mutan, sehingga perlu dilakukan pemeriksaan status mutasigen KRAS. Telitian berupa deskriptif dengan pendekatan potong lintang yang bertujuan untuk mendapatkan data status mutasi genKRAS kodon 12 dan 13 di pasien adenocarcinoma colorectal. Deteksi mutasi KRAS dilakukan dengan teknik Polymerase Chain ReactionRestriction Fragment Length Polymorphism (PCR RFLP) yang dikonfirmasi dengan sekuensing. Sampel telitian adalah 30 blok parafinyang diperoleh dari Rumah Sakit Dr.Soetomo Surabaya masa waktu Januari-Desember 2013. Setelah dilakukan ekstraksi DNA terdapat21 sampel yang dapat digunakan untuk pemeriksaan lanjutan. Hasil PCR RFLP menunjukkan terdapat 7/21 mutasi pada kodon12 dan tidak terdapat mutasi gen KRAS pada kodon 13. Mutasi pada kodon 12 yaitu GGT>GCT, GGT>GGA dan GGT>GAT yangmenyebabkan perubahan asam amino Gly12Ala, Gly12Gly dan Gly12Asp. Simpulan telitian ini adalah mutasi gen KRAS kodon 12 padaadenocarcinoma colorectal di Rumah Sakit Dr. Soetomo Surabaya sebanyak 33%.

INCREASING DURATION OF FERTILITY IN WOMEN WITH ENDOMETRIOSIS Fitria Nengsih; Jimmy Yanuar Annas; Reny I'tishom
Indonesian Midwifery and Health Sciences Journal Vol. 5 No. 4 (2021): Indonesian Midwifery and Health Sciences Journal, October 2021
Publisher : UNIVERSITAS AIRLANGGA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/imhsj.v5i4.2021.359-366

AbstractIntroduction: Infertility is one of the common clinical symptoms in women with endometriosis. Limited ways to diagnose endometriosis, symptoms of endometriosis considered normal and overlap with other diseases that cause delaying treatment that increase the duration of infertility. This study purpose to analyze the differences in duration of infertility to women with endometriosis and without endometriosis. Method: This study is observational analytical research with retrospective case-control designed by looking at medical records in Fertility Clinic Graha Amerta RSUD Dr. Soetomo Surabaya. Total samples in this research are 50 women with infertility, it is divided into 25 people with endometriosis and 25 people without endometriosis. In the medical records, we can see the duration of infertility in each sample. Results: The duration of infertility >3 years occurs in women with and without endometriosis (72% and 80%). In bivariate analysis using the chi-square test, there was no difference that showed increasing duration of infertility (P = 0.508) between women with endometriosis and without endometriosis. Conclusion: There was no differences in the duration of female infertility with endometriosis and without endometriosis

Co-Authors A. Marlinata Abadiyah Zakiah Kustantina Abdurachman Abdurachman Addia Salsabila Ade Septiari Rahman Adha, Ahmad Zaidan Ni'am Abu Aditiawarman Aditiawarman, Aditiawarman Ahmad Basori Ahmad Ricardo Silalahi Ahmad Ricardo Silalahi Ahmad Ricardo Silalahi Alphania Rahniayu Ami Ashariati Anak Agung Istri Dalem Cinthya Riris Anak Agung Istri Dalem Cinthya Riris Anak Agung Renjani Kanya Kumari Andi Yasmin Wijaya Andri Rezano Anggi Dearni Silalahi Anggita Putri Samara Annas, Jimmy Yanuar Anny Setijo Rahaju, Anny Setijo Arif Rananda Arifa Mustika Arrasy Khawarizmi Ashon Sa’adi Aslama, Fathiy Zakaria Asmalinda, Wita Astri Dewayani Atmaja, Maxwell Aucky Hinting Ayik Rochyatul Jannah Ayik Rochyatul Jannah Azarine Ariqoh Arysanti Bahyshidqi Bambang Purwanto Budi Sutikno Caesariska Deswima Christian Christopher Sunnu Clara Alverina Daniel Widiyanto David Setyo Budi Diana, Alis Nur Dienanta, Savira Butsainah Dita Mega Utami Doddy M Soebadi Dwiki Noni Armyta Dzakiyyah, Nurul Ebnudesita, Faiza Rahma Eddy Bagus Wasito Era Fazira Etik Yuliarini Widodo Evy Wulandari Evy Wulandari Faiza Rahma Ebnudesita Faiza Rahma Ebnudesita Faizah Sugiarto Fedik Abdul Rantam Feranita Kumalasari Fitri Indah Pratiwi Fitria Nengsih Gadis Meinar Sari Gondo Mastutik Hamdani Lunardhi Hanik Badriyah Hidayati,* Mohammad Hasan Machfoed,* Kuntoro,** Soetojo,*** Budi Santoso,**** Suroto,***** Budi Utomo****** Hanna Tabita Hasianna Silitonga Hendy Hendarto HERAWATI, LILIK Hermina Novida, Hermina Heru Fajar Trianto Hudi Winarso Inda Corniawati Indra Alfaray, Ricky Intan Indah Permatasari IRWANTO Isnin Anang Marhana James Alfedo Jannah, Ayik Rochyatul Jefry Albari Tribowo Jihadna Prima Santika Ruslan Musanip Kartika Afrida Fauzia Kevin kevin Khaerunnisa, Siti Lionardy Yodianto Lukman Hakim Magfira Febrianty Lacindung Maria Magdalena Awi Mochamad Amin Mochammad Abdul Aziz, Mochammad Abdul Mohammad Anam Al-Ari Mohammad F. Qorib Mohammad Fathul Qorib Muhammad Miftahussurur Mustafa, Mohammad R. Nathania Nathania Nathania Nila Kurniasari Ninik Darsini Nurul Jannatul Wahidah Nurul Jannatul Wahidah Nurwasis Nurwasis Nyilo Purnami Purnama, Michael Purwanti, Dwi Puspitasari, Renny N. Qurnianingsih, Ema Rafida Anshori Raharjo, Rahmawati Rejeki, Purwo Sri Reryd Arindany Wiryawan, Reryd Arindany Ria Margiana Ria Margiana Rima Wirenviona Rima Wirenviona Rizaldi, Fikri Rize Budi Amalia Rizkiliano, Ibrahim Hanif Rochmah Kurnijasanti Rossy Sintya Marthasari S.Pd. M Kes I Ketut Sudiana . Safira Nur Izzah Salsabila Rahma Nurani Putri Salsabila, Unik Hanifah Sandra Dewi Sitaresmi Savira Butsainah Dienanta Savira Butsainah Dienanta Savitri, Camilia Metadea Aji Selvina Cindy Kusumaningrum Shahnaz Azzahra Siti Khaerunnisa Soebadi, Doddy Soetojo Sofia Zahra Kamila Sri A. Sudjarwo Sri Agus Sudjarwo Sri Purwaningsih Sri Ratna Dwiningsih Steven Sheng Looi Sudana, Nathan Kunta Sudjarwo, Sri A. Suhartono Taat Putra Sunaryo Hardjowijoto Supardi Supardi Susanti, Nurul Fatimah Suyono, Seso Sulijaya Syahnural Syahnural Syarifah, Anis Satus Tanojo, Tjahjo Djojo Tjahjo Djojo Tanojo Tjahjo Djojo Tanojo Tutus Rachkutho Vania Islamey Kusuma wahjoe djatisoesanto Wahyul Anis Widjayanti, Yhenti Widya Juwita WINARTO Winona May Hendrata Yassin, Tita Rudini Yaudiwati, Rina Yohana djurumana Yoshio Yamaoka, Yoshio Yudith Annisa Ayu Rezkitha, Yudith Annisa Ayu Yudiwati, Rina Yuliawati, Tri Hartini Zuhria, Ismi

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