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Hasim
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Journal : Current Biochemistry

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Kandungan Nutrisi dan Daya Inhibisi α-glukosidase Ekstrak Daging Buah Salak Sidempuan (Salacca sumatrana) Safithri, Mega; Ritonga, Aprilita Putri Defan; Indariani, Susi; Dwicesaria, Maheswari Alfira; Sulistiyani; Hasim
Current Biochemistry Vol. 11 No. 1 (2024)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.11.1.1

Abstract

Penelitian terhadap pemanfaatan salak khususnya salak Sidempuan sebagai inhibitor α-glukosidase belum dilakukan. Penelitian ini bertujuan mengukur kadar proksimat (air, abu, protein kasar, lemak kasar, serat kasar, dan karbohidrat), menganalisis komponen fitokimia, kandungan total fenolik dan aktivitas penghambatan α-glukosidase pada ekstrak air dan etanol daging buah salak Sidempuan. Kadar total fenol diukur menggunakan metode Folin Ciocalteu. Aktivitas penghambatan α-glukosidase diukur menggunakan substrat pNPG. Kadar karbohidrat, air, abu, lemak kasar, protein kasar, dan serat kasar berturut-turut sebesar 86.35%, 6.57%, 3.60%, 1.44%, 1.24%, dan 0.82%. Kedua ekstrak mengandung senyawa flavonoid dan saponin. Ekstrak etanol 70% daging buah salak Sidempuan memiliki kandungan total fenol sebesar 10.6 mg GAE/g ekstrak, lebih tinggi dibandingkan ekstrak air sebesar 5.55 mg GAE/g esktrak. Ekstrak etanol daging buah salak Sidempuan memiliki konsentrasi inhibisi (IC50) sebesar 13.69 mg/L. Konsentrasi ini lebih kecil dibandingkan ekstrak air yaitu 4160.56 mg/L, namun masih lebih besar dibandingkan akarbosa yaitu 1.15x10-3 mg/L sebagai kontrol positif.
Antibacterial Activity of Hermetia illucens (Black Soldier Fly) Maggot Extract Against Salmonella typhi Andrianto, Dimas; Hasim; Muhammad, Zulfikar; Dwicesaria, Maheswari Alfira
Current Biochemistry Vol. 12 No. 2 (2025)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.12.2.1

Abstract

Typhus is an acute fever caused by Salmonella typhi bacterial infection. This research aims to test the efficacy of BSF maggot extract as an antibacterial against Escherichia coli and Salmonella typhi. The stages in this research are the preparation of maggot samples, determination of water content of samples, extraction of maggot BSF, screening of antibacterial activity of maggot BSF against Escherichia coli and Salmonella typhi, fractionation from the best results of antibacterial activity of maggot extract BSF and the test of antibacterial activity of maggot BSF fraction against Escherichia coli and Salmonella typhi, fractionation of the best results of antibacterial activity of maggot extract BSF and the test of antibacterial activity of maggot BSF fraction against Escherichia coli and Salmonella typhi. Extraction was carried out by a multi-level maceration method using acetone, methanol and water solvents, then the extract was tested for antibacterial activity against Salmonella typhi using the disc diffusion method with the best results, acetone extract with an inhibition zone of 8.13 mm ± 0.153. Then, acetone extract was fractionated according to its polarity, namely n-hexane, dichloromethane, ethyl acetate, and water. The results of the fractionation were tested for antibacterial activity against Escherichia coli and Salmonella typhi using the disc method with the best fraction was the n-hexane fraction, at a concentration of 2500 ppm with inhibition zones 8.37 mm ± 0.32 (Escherichia coli) and 9.00 mm ± 0.00 (Salmonella typhi)
Co-Authors -, Ahmad Faqih Agus Setiyono Agus Soroso Ahmad Multazam, Ahmad Ahmad Sulaeman Ahmad Zainuri Andi Sofyan Hasdam Anid Supriyadi Ario Damar Assel, Abdurrahman Astuti, Sherly Awalia, Nur Bagu, Fery Rahmat A. Baruadi, Mahludin H. Daud Yusuf Desi Widiyanti Dewi Wahyuni K. Baderan Diko, Abdullah Kadir DIMAS ANDRIANTO Dwicesaria, Maheswari Alfira Eko Pradjoko Ery Setiawan Evy Damayanthi Farid SM, Farid Fera Fitri Salsabila Fitryane Lihawa Gita Eka Sila Humairoh Saidah I Dewa Gede Jaya Negara I Wayan Yasa Idqan Fahmi Indariani, Susi Insih Mohune IPB, DGB Irawan, Mohammad Bayu Irvan A. Salihi Iswan Dunggio Jahja, Sukma Dewanty Karyawan, I Dewa Made Alit Katili, Ardiyansyah S. Kurniawati, Estetika Mutiaranisa Lala M Kolopaking Lihawa, Fityane M Agus Setiadi Ma'mun Sarma Mahludin H. Baruadi Mahludin H. Baruwadi Malik, Safira Putri H. Marike Mahmud Mega Safithri Moh. Hasan Machfoed Mohamad, Nurdin Moko, Faisal Musafir Nahrowi Noi, Siskawati Nufalia Hidayati Nurfaika Nurislamiah, Siti Oktariza, Wawan Pakaya, Parid Prasetyo, Mawardi Heru Pujiati Purwiyatno Hariyadi Rachmiani Rahayu Yulianti Rahman, Sitti Mutiah Rahmawati Damiti Rakhmat Jaya Lahay Ritonga, Aprilita Putri Defan Rohani Rubai, Ramlah Sabarudin, Didin Salamatun Asakdiyah, Salamatun Salehudin Setyawati, Ariesta Sri Purwaningsih, Sri Sudradjat Suherman, Sutianto Pratama Sukirman Rahim Sulistiyani Sumiati Suria Darma Tarigan Sutianto Syam S. Kumaji Syamsudin, Suryadi Ulfah Juniarti Siregar Waode, Faridawaty Wimadudin Z Thalib, Heru Zulfikar Muhammad, Zulfikar