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All Journal AKSIOMA: Jurnal Program Studi Pendidikan Matematika Jurnal Biologi Tropis Tunjuk Ajar: Jurnal Penelitian Ilmu Pendidikan Jurnal PIPSI: Jurnal Pendidikan Ilmu Pengetahuan Sosial Indonesia Pendas : Jurnah Ilmiah Pendidikan Dasar SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Martabe : Jurnal Pengabdian Kepada Masyarakat Journal on Education EDUKATIF : JURNAL ILMU PENDIDIKAN Jurnal Educatio FKIP UNMA Jurnal Ilmiah Profesi Pendidikan PROGRES PENDIDIKAN Journal of Classroom Action Research Jurnal Pendidikan, Sains, Geologi, dan Geofisika (GeoScienceEd Journal) Journal of Educational Review and Research Mitra Mahajana: Jurnal Pengabdian Masyarakat Renjana Pendidikan Dasar Jurnal Ilmiah Wahana Pendidikan LEARNING : Jurnal Inovasi Penelitian Pendidikan dan Pembelajaran SOCIAL : Jurnal Inovasi Pendidikan IPS Jurnal Interaktif: Warta Pengabdian Pendidikan (JIWPP) Jurnal Pengabdian Kepada Masyarakat Patikala Empiricism Journal Jurnal Warta Desa (JWD) DEDIKASI : JURNAL PENGABDIAN KEPADA MASYARAKAT Jurnal Profesi Keguruan
Lalu Wira Zain Amrullah
Program Studi PGSD FKIP Universitas Mataram
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Journal : Jurnal Biologi Tropis

 1 
Screening and Molecular Identification of Phosphate-Solubilizing Rhizobacteria from Mangrove Ecosystem of the Lombok Island Lalu Zulkifli; Prapti Sedijani; Dewa Ayu Citra Rasmi; Lalu Wira Zain Amrullah
Jurnal Biologi Tropis Vol. 20 No. 3 (2020): September - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v20i3.1730

Abstract

Phosphate solubilizing rhizobacteria can be used as a component in biofertilizer formulations to increase local and national food production without causing adverse risks to the environment compared to the use of chemical fertilizers. In this regard, screening and identification of phosphate solubilizing bacteria from the rhizosphere of several mangrove species that grow on the coast of Lombok Island has been carried out. The method of isolation used is the Pikovskaya method. Screening and identification activities have obtained 5 isolates of mangrove rhizosphere bacteria (BRM) which are indicated by their ability to form clear zones on Pikovskaya media, namely isolates BRM1 and BRM4 (isolated from the rhizosphere of Avicennia marina), BRM2 and BRM3 (isolated from the rhizosphere of Rhizophora apicullata), BRM5 (isolated from the rhizosphere of R. stylosa). All rhizobacteria isolates were identified as Gram-positive bacteria. Molecular analysis based on the comparison of 16S rRNA gene sequences of isolates with the genome database at GenBank (NCBI) using Mega 10 software, showed that all BRM isolates occupied the same cluster as bacterial species from the Genus Paenibacillus in the dendrogram of the phylogenetic tree, namely Paenibacillus sp. JWLB1 strain, Paenibacillus sp. Strain NO13, P. cineris strain cu1-7, P. favisporus strain CHP14, with genetic distance ranging from 1.3 to 1.4. Many species of the Genus Paenibacillus are currently known to play an important roles as plant growth-promoting bacteria. The BRM isolates obtained in this study can be further developed as a biofertilizer component (inoculant) in saline and dryland agriculture.
DNA Barcoding of Endophytic Bacteria from The Stems of Tissue Piper crocatum and Activity Anti-Bacterial Properties Lalu Wira Zain Amrullah; Lalu Zulkifli
Jurnal Biologi Tropis Vol. 22 No. 4 (2022): October - December
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v22i4.4299

Abstract

Secondary metabolites from herbal are commonly used to treat disease due to infection from pathogenic bacteria, endophytic bacteria is shortcut to collect secondary metabolites more effective than extracting direct from the plant. This research aims to obtain endophytic bacterial isolates from the stems of the red betel plant which can be used as an inhibitor for the growth of pathogenic bacteria. The method of this research is exploratory descriptive experiment based research. The stages of this research consist of few techniques which are isolation of endophytic, antibacterial examination, morphological, physiological, and biochemical observation, DNA bacterial extraction, gel electrophoresis. To analyse DNA Barcoding using 16S rRNA gen, BLAST and MEGA 10. The results of the antibacterial activity of the isolates showed by clear zone formation against pathogenic bacteria such as E. coli, K. pneumoniae and S. aureus. Endophytic bacterial that successfully isolated are labelled (BSM1, BSM2, BSM3 and BSM4). Based on of the Gel electrophoresis shows that four endophytic bacterial isolates are positive by appearance of bands in 1324bp. Based on the results of the phylogenetic tree it can be concluded that BSM1 is closely related to Bacillus cereus JL with a genetic distance of 0.007, while the genetic distance of BSM2 was 0.012, BSM3 0.006 and BSM4 0.003, all three were closely related to Bacillus pumilus AUEC29.
Co-Authors Adelia Noviyanti Aisa Nikmah Rahmatih Aisa Nikmah Rahmatih Anggrawan, Bunga Safira Arif Widodo Asri Fauzi Asri Fauzi Asrin Asrin Asrin Asrin Aulia Dwi Amalina Wahab Aulia, Vila Awal Nur Kholifatur Rosyidah Ayu Aina Nilna Nur Baiq Niswatul Khair Dewa Ayu Citra Rasmi Dewi, Eva Rosdiana Diana Novita Putri Dwina Angga, Prayogi Dyah Indraswati Fauzi, Asri Fazriyanti, Vita Febriliana, Weni Filsa Era Sativa, Agil Al Idrus, Gito Hadiprayitno Gita Prima Putra Hasnawati Hasnawati Hasnawati Hasnawati Hasnawati Hasnawati Heri Hadi Saputra Husniati Husniati, Husniati I Ketut Widiada I Nyoman Karma I Nyoman Karma I Nyoman Karna Ida Bagus Kade Gunayasa Ida Ermiana Ilham Handika Indraswati, Dyah Irawan Zain, Moh Itsna Karma Itsna Oktaviyanti Karma, I Nyoman Kartika Astuti, Nofita Kelandia, Isfawani Khairun Nisa Khairun Nisa Kusuma, Tiara Lalu Zulkifli Lalu Zulkifli Linda Feni Haryati Liwa Ilhamdi, Mohammad Maftuhah, Tauri Makki, Muhammad Mansur Hakim Moh. Irawan Zain Mohammad Archi Maulyda Muhammad Erfan Muhammad Erfan Muhammad Erfan, Muhammad Muhammad Irawan Zain Muhammad Sobri Muhammad Sobri Muhammad Sobri Muhammad Syazali Muhammad Tahir Muhammad Tahir Muhammad Tahir Musaddat, Syaiful Novitasari, Setiani Nur'aini Nurhasanah Nurhasanah Nurhasanah Nurhasanah Nurhasanah Nurmawanti, Iva Nurul Kemala Dewi Nurwahidah Nurwahidah Prapti Sedijani Putra, Gita Prima Putri, Hikmah Ramdhani Qadrifuddin, Taufiq Al Ashfahani Radiusman Radiusman Radiusman, Radiusman Rahmatih, Aisa Nikmah Rahmawati Ramadhani, Miatun Rizaldi, Imam Robiah, Hanifatul Saskia, Ainur Setiani Siti Istiningsih Siti Istiningsih, Siti Sudirman Sudirman Sudirman Sukri sukri Sukri, sukri Syazali, Muhammad Thaharah, Ainun Tia Ilda Annisa Umar Umar Umar Umar Vira Sasmita Vivi Rachmatul Hidayati Widodo, Arif Wijaya, Tami Amalia Zain, Muhammad Irawan