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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Ilmu Ternak Veteriner AGRICOLA Animal Production : Indonesian Journal of Animal Production Biosaintifika: Journal of Biology & Biology Education Jurnal Medik Veteriner Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) ARSHI Veterinary Letters JURNAL KAJIAN VETERINER Indonesian Journal of Applied Research (IJAR) Jurnal Kedokteran Hewan Chalaza Journal of Animal Husbandry Policy Brief Pertanian, Kelautan, dan Biosains Tropika Jurnal Agripet Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia

R. Iis Arifiantini

Divisi Reproduksi Dan Kebidanan, Departemen Klinik, Reproduksi, Dan Patologi, Fakultas Kedokteran Hewan, IPB, Jl Agatis, Kampus IPB, Dramaga, Bogor, Jawa Barat, Indonesia,16880.

Author-ID : 2480415

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Karakteristik Semen dan Korelasi antara Konsentrasi Testosteron dengan Libido Pejantan Sapi Simental Abdullah Baharun; Syahruddin Said; R. Iis Arifiantini; Ni Wayan Kurniani Karja
Acta VETERINARIA Indonesiana Vol. 10 No. 1 (2022): Maret 2022
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.10.1.1-7

Penelitian ini bertujuan untuk mempelajari karakteristik semen dan mengevaluasi hubungan antara konsentrasi testosteron dengan libido pejantan sapi Simental. Sebanyak 12 ekor sapi Simental, 9 ekor dengan motilitas spermatozoa ≥70% (A) dan 3 ekor dengan motilitas spermatozoa <70% (B) berdasarkan data sekunder dari Balai Inseminasi Buatan (BIB) tahun 2018-2019. Semen ditampung menggunakan vagina buatan pada pagi hari. Evaluasi kualitas semen segar hanya dilakukan dengan penilaian morfologi spermatozoa. Sampel darah disentrifugasi (3000 rpm, 10 menit), supernatan dimasukkan ke dalam microtube dan disimpan pada suhu -20 °C. Analisis testosteron menggunakan metode ELISA. Data kualitas semen segar sapi pejantan Simental dianalisis secara deskriptif. Korelasi antara karakteristik semen, libido dan konsentrasi hormon testosteron menggunakan korelasi Pearson’s. Hasil analisis menunjukkan terdapat perbedaan signifikan (P<0,05) antara kualitas semen segar kelompok pejantan A dengan pejantan B dalam parameter motilitas, konsentrasi, dan morfologi spermatozoa normal. Volume semen dan skor libido pada semua pejantan tidak menunjukkan perbedaan signifikan. Konsentrasi testosteron pada kelompok pejantan A lebih tinggi (42,57 ng/mL) dibandingkan dengan kelompok pejantan B (33,26 ng/mL). Konsentrasi testosteron menunjukkan korelasi positif (P<0,01) dengan karakteristik semen seperti motilitas spermatozoa (0,813), morfologi spermatozoa normal (0,639), dan libido (0,952). Hasil penelitian ini dapat digunakan sebagai pertimbangan untuk dilakukannya pengukuran konsentrasi testosteron dalam proses seleksi sapi jantan menjadi pejantan.

FA-6 Analysis of Sperm Freezing Capability of Various Bulls at the Singosari AI Center Rhesti Indriastuti; Enniek Herwiyanti; Anny Amaliya; Iis Arifiantini; Bambang Purwantara
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (427.268 KB)

Artificial insemination (AI) has been widely applied to improve genetic quality in cattle worldwide. One of the most important factors in AI program is the quality of semen. Many procedures in semen processing have been developed to preserve the sperm quality. The Singosari AI center produces the frozen semen through cryopreservation method. During this process, the number of semen collected will be rejected if the quality is low. Fresh semen with less than 70% of sperm motility, before freezing semen with less than 55%of sperm motility and less than 40% of sperm post-thawing motility evaluation will be rejected, so that it was only the viable sperm will be processed to be frozen semen commercial. Therefore, comparative study was carried out to analyze the freezing capability of sperm.

Genetic and Non-Genetic Effects on Semen Characteristics of Bali Cattle (Bos javanicus) Gunawan Sitanggang; Raden Iis Arifiantini; J Jakaria
Jurnal Ilmu Ternak dan Veteriner Vol 25, No 4 (2020): December 2020
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v25i4.2526

The objective of this study was to evaluate effect of genetic and non-genetic factors on semen characteristics including ejaculate volume, sperm concentration, total sperm number and sperm moetility of Bali cattle. Semen data were collected from the National Centre of Artificial Insemination at Singosari, Malang, East Java, Indonesia. A total of 3,847 ejaculates of 17 Bali bulls from 2014 to 2016 were collected and evaluated. Data were analyzed by restricted maximum likelihood (REML) method using mixed models which the bull was a random effect, while age of bull, season of collection, frequency of ejaculation and collection intervals were the fixed effects. Results showed that age significantly affected all semen characteristics (P<0.01). Season affected only on sperm motility (P<0.01). Effect of frequency of ejaculation and collection intervals was significant on all studied variables (P<0.01), except sperm motility. Repeatability of ejaculate volume, sperm concentration, total sperm number and sperm motility was 0.43; 0.35; 0.32 and 0.31, respectively. It is concluded that age, frequency of ejaculation and collection intervals were the most factors affected semen characteristics of Bali cattle. Repeatability estimations of semen characteristics were moderate to high.

Pengaruh Pakan Limbah Tauge Dan Suplementasi Omega-3 Terhadap Produksi Spermatozoa Domba Garut Nurcholis Nurcholis; Raden Iis Arifiantini; Mohamad Yamin
AGRICOLA Vol 5 No 2 (2015): AGRICOLA
Publisher : Universitas Musamus, Merauke, Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35724/ag.v5i2.419

The objective of the research was to analyze the feeding effect of bean sprout waste and omega-3 to the spermatozoa production of garut rams. Twelve garut rams were divided into three groups of feeding; grass + concentrate (R1), bean sprouts waste + concentrate (R2) and bean sprouts waste + concentrate + omega-3 (R3). The semen were collected by using artificial vagina once a week. Semen evaluated macroscopically (volume, color, consistency and pH) and microscopically (mass movement, motility, viability, concentration, concentration per ejaculate and morphology of spermatozoa).The research was designed by using a completely randomized design (repeated measurement) and statistically analyzed using SAS 9.1 software. The results showed macroscopy no differences was found on microscopyc evaluation except for semen volume. Microscopyc evaluation showed that motility, viability and spermatozoa concentration as well as total number per ejaculete feed with R2dan R3 were highger (P<0.05) than R1. However supplementation of omega-3 was no better than feed containing bean sprouts waste.

Pengembangan Metode Identifikasi Kerusakan DNA Spermatozoa Ternak Teguh Ari Prabowo; R. Iis Arifiantini; Dondin Sajuthi; Uus Saefullah
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The success of artificial insemination is very much determined by the quality of spermatozoa. The detection or identification of damaged chromatin of spermatozoa DNA is very important to forsee the adverse clinical outcome. However, the method of identification is still depended on expensive imported kits. Therefore, the objective of this research was to developed an identification kit to determine the quality of livestock spermatozoa DNA chromatin.This study consist of three step. Step 1) Determination of low melting point agarose (LMP-agarose) concentration which is 0,6%, 0,7% and 0,8%. 2) Comparison of three lysis solution (LS) which is LS I (0.4M Tris, 0.8M DTT, 1% SDS, pH 7.5), LS II (0.4M Tris,2 M NaCl, 1% SDS , pH 7.5), and LS III (0.4M Tris-HCl, 2M NaCl, 1% SDS 0,05 M EDTA, pH 7.5). 3) Comparison different staining which is Eosin yellow and Methylene blue. The results showed that 0.6% LMP-agarose demonstrated the best concentration to “trapped the spermatozoa” compared for sheep and goats. whereas the three concentration of spermatozoa cows can not be used to trap spermatozoa cow. The best formulation to lysis the membrane was LS III (0.4M Tris -HCl, 2M NaCl, 1% SDS 0,05 M EDTA). The best staining was eosin yellow and methylene blue with 2:1 ratio.

Semen Quality of Sentul Cock with Different Immunoglobulin Yolk Concentrations Ria Ariyanti; Niken Ulupi; Tuti Suryati; Raden Iis Arifiantini
Buletin Peternakan Vol 43, No 4 (2019): BULETIN PETERNAKAN VOL. 43 (4) NOVEMBER 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v43i4.37051

The aim of this study was to evaluate the effect of different IgY concentrations on the quality of semen (macroscopic and microscopic) and testicular morphometry (weight, length and width of the testes) of Sentul cock testis and its ability to fertilize hens. This study used 4 months of 20 Sentul cocks consisting 10 high IgY concentration cocks and low IgY concentration cocks. This study used a complete randomized design (RAL) with 2 treatments namely low and high IgY concentration and10 times repetitions. Data were analyzed using t-test minitab program versions 16. Based on the result, different IgY concentrations did not affect the quality of semen and testicular morphometrics, but high IgY concentration cocks were able to fertilize more hens than the low IgY concentrations one by using dilution methods. This proves that high IgY concentration cocks are more effective and efficient cocks or stud.

Kualitas Semen Beku Kuda pada Pengencer Susu Skim dengan Konsentrasi Gliserol yang Berbeda Azizah -; Raden Iis Arifiantini
Jurnal Veteriner Vol 10 No 2 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (161.608 KB)

Cryoprotectant is one of determining factors for the success of stallion semen cryopreservation. Formost mammalian sperm cryopreservation,glycerol has been widely used as the cryoprotectant. Glycerol inhigh concentration is toxic to the sperm but in low concentration it has protective effect on the semen. Theobjectives of this experiment were to find appropiate glycerol concentration by using skim milk extender inorder to maintain motility and viability of spermatozoa of stallion frozen semen. Semen was collectedfrom three sexually mature stallions by using artificial vagina. The semen characteristics and qualitywere examined both macro and microscopically. Prior to extension, semen was centrifugated at 3000 rpmfor 15 minutes. The pellet was re-extended with skim milk extender with glycerol 5% (G5) 7.5% (G7.5), and10% (G10). The extended semen as then packed in mini straw (0.3ml), equilibrated at 5 oC for 2 hours,frozen in the liquid N2 vapor for15 minutes and then stored in liquid N2 container until futher evaluation.The frozen semen, was thawed at 37 oC for 30 seconds. The percentages of sperm motility and viabilitywere examihed. The result of this research showed that the sperm motility and viability in G5 was (23.8 %;35.6 %) significantly hihger (P<0.05) as compare a with G7.5 (18.1%; 28.6%) and G10. (11.8%; 23.1%). Therecovery Rate in G5 (35.4%) was significant high er (P<0.05) than G7.5 (26.9%) and G10 (17.6%). In conclusion,glicerol of 5% is recomended for cryopreservation of stallion semen extended with skim milk.

Kualitas Semen Kambing Sapera yang Dibekukan dalam Pengencer Tris Kuning Telur dengan Imbuhan Pentoxifylline (QUALITY OF SAPERA BUCK SEMEN FROZEN IN TRIS EGG YOLK EXTENDER ADDED WITH PENTOXIFYLLINE) Bq Hayyul Hidayati; Raden Iis Arifiantini; Ni Wayan Kurniani Karja; Diana Andrianita Kusumaningrum
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (117.717 KB)

The aim of this study was to determine the effective dose of pentoxyfilline (PTX) to improve the quality of sapera buck frozen semen in Tris Egg Yolk (TEY) extender. Two sexually mature sapera bucks aged 1.5 years old was used as semen source. Semen collected with artificial vagina. Fresh semen were evaluated macro and microscopically. Only ejaculates showing > 70% sperm motility were selected. Semen were divided into four aliquots tubes containing TEY extender with 0 mM, 3.5 mM, 5 mM and 6.5 mM PTX each tube. After dilution, semen were packed into mini straw (0.25 ml) and equilibrated at 5 oC for 4 hours, then frozen above liquid nitrogen vapors for 10 minutes before plunged into liquid nitrogen (-196 oC). Results of this study showed that 6.5 mM PTX addition to the TEY extender improve post-thaw semen motility (P<0.05) but not viability and plasma membrane integrity. It concluded that 6.5 mM PTX addition to TEY extender improved post-thaw sperm motility of sapera buck.

KUALITAS SEMEN SEGAR SAPI BALI PADA DUA KELOMPOK UMUR DAN PENENTUAN KONSENTRASI KRIOPROTEKTAN DALAM PENGENCER TRIS KUNING TELUR (Fresh Semen Quality Of Bali Bull In Two Group Of Age And Determination Of Cryoprotective Agent Concentration In Tris Eggyolk Extender) Anna Nabilla; Raden Iis Arifiantini; Bambang Purwantara
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The aim of this study was to compare the quality of Bali bull fresh semen in productive group (below 10 years old) with over productive group and to evaluate the frozen semen quality of bali bull after cryopreservation in Tris egg yolk extender using Dimethyl formamide (DMF) and glycerol in three different concentrations (5%, 6%, and 7%). Semen was collected using artificial vagina. Semen then evaluated macro and microscopically. Macroscopic evaluation conducted visually and microscopic evaluation with the aid of Computer Assisted Sperm Analysis CASA. Immediatelly after evaluation, semen were divided into six aliquots and diluted with Tris egg yolk (TEY) glycerol 5% (TEYG5), TEY-glycerol 6% (TEYG6), TEY glycerol 7% (TEYG7), TEY-DMF 5% (TKTD5), TEY-DMF 6% (TEYD6) and TEY-DMF 7% (TKTD7). Results of experiment showed that there was no difference (P>0.05) among the all parameters, except for semen volume. Semen volume of productive group was higher than over productive age group. No difference was found in post thawing motility of the spermatozoa in TEY extender with 5% glycerol, and TEY with 5%, 6% and 7% DMF. The higher recovery rate of bali bull spermatozoa demonstrated by spermatozoa in TEY extender with glycerol 5% and DMF 6% (63.33±2.40 dan 68.67±2.33). It was concluded that bali bull semen quality did not differ between productive and over productive ages and post thawing semen quality of bali bull in TEY with 5-7% DMF or 5% glycerol was not also different.

Penentuan Siklus Estrus Berdasarkan Gambaran Sitologi Vagina dan Profil Hormon pada Rusa Timor Wilmientje Marlene Mesang Nalley; Ristika Handarini; Muhammad Rizal; Raden Iis Arifiantini; Tuty Laswardi Yusuf; Bambang Purwantara
Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (213.983 KB)

Determination of estrous cycle especially the length of estrous is an important role in improvingthe fertility and reproductive performance of farm animals. This study was aimed to observe thelength of estrous cycle and estrous period based on cytologic and estrogen (E2) and progesterone (P4)hormone profiles. Samples of vaginal smears were collected daily and stained with Giemsa. Bloodsamples were collected every two days using jugular vein implant catheter. The hormone profileswere analized by radio immuno assay. All data were analyzed descriptively. Results of this studyshowed that there were four lowest points percentage of parabasal epithelial cells with the averageof 14.25%, while the superficial epithelial cells showed four highest points (85.75%). The intervalsbetween lowest point of parabasal epithelial cell and the highest point of superficial cells were at 20,18, and 16 with the average of 18 days. This phenomenon showed a cyclical processed with almostthe same length of time of the actual an estrous cycle pattern in timor deer. The range of E2 concentration was 7.06 to 18.14 pg/ml and P4 concentration was 2.58 to 7.48 ng/ml. The intervaltime between the peak of E2 was 17 days which represented the estrous cycle of timor deer. It isconcluded that analysis of estrous cycle in timor deer can be detected by vaginal cytological andhormone analysis profiles.

Co-Authors . Soeparna A.A. Ketut Agung Cahyawan W Abdullah Baharum Abdullah Baharun Agasi, Satri Yusasra Amani, Aqila Zata Aminudin Aminudin Anak Agung Gede Sugianthara Andriani Andriani Angelina N. Tethool Anna Nabilla Anny Amaliya Asep Gunawan Asep Kurnia Asep Kurnia Azizah - Baharum, Abdullah Bambang Purwantara Bq Hayyul Hidayati Cece Sumantri Damiana Rita Ekastuti Dedi Rahmat Setiadi DEWI APRI ASTUTI Diana Andrianita Kusumaningrum Dondin Sajuthi Eko Prasetyo Nugroho Elmanaviean, Muhammad Emilia Kamung Hambu, Emilia Kamung Enniek Herwiyanti Faisal Amri Satrio Frederika Katerina Foeh, Nancy Diana Gatot Prasojo GONO SEMIADI Gunawan Sitanggang Gusdinar, Rizal Hasbi Hasbi Hastuti, Yohana Tri Henny Nuraini herdis herdis Herry Sonjaya Hikmayani Iskandar Imam Purwadi, Imam Iman Supriatna Indriastuti, Rhesti Jakaria Jakaria Jansen Manansang Jansen Manansang Jean-Baptise Menassol Junaedi Junaedi Junaedi Junaedi Ketut Adnyane Mudite Khaeruddin Khaeruddin Khaeruddin Khaeruddin Komariah Komariah Kurnia Bagus Ariyanto Kusdiantoro Mohamad Laksono, Mohamad Nanang Tejo Langgeng Priyanto Lilis Khotijah Magfira Magfira Magfira Magfira Magfira Maghfira Maghfira Manansang, Willem Memili, Erdogan Mohamad Yamin Mohamad Yamin Mohamad Yamin Mokhamad Fahrudin MUHAMMAD AGIL Muhammad Agus Tahar Muhammad Gunawan Mulia, Bongot Huaso Muttaqinullah Rabusin Nancy D. F. K. Foeh Ni Wayan Karniani Karja Ni Wayan Kurniani Karja Niken Ulupi Nila Pratiwi Nugraha, Arifin Budiman Nur Hidayati Nurcholis Nurcholis Nurcholis Nurcholis Nurcholis Nurcholis Nuri Andarwulan Oriza Savitri Ariantie Palupi, Nurheni Sri Pardede , Berlin Pandapotan Pardede, Berlin Pandapotan Prayetno, Saptohadi Puwantara, Bambang Rahmat Hidayat Rahmat Hidayat Reni Ratni Dapawole Rhesti Indriastuti Rhomadon, Gilang Ria Ariyanti Ristika Handarini Riwu, Ronny Markus Jami Rudi Afnan Santoso Santoso Setyaningsih Rambu Liwa Siti Zubaidah Soeparna Soeparna Sri Darwati Sri Darwati Srihadi Agungpriyono Syahruddin Said Teguh Ari Prabowo Tejolaksono, Muhammad Nanang Tike Sartika Tike Sartika Triansyah, Irvan Tulus Maulana Tuti Suryati Tuty Laswardi Yusuf Tuty Laswardi Yusuf TUTY LASWARDI YUSUF Tuty Laswardi Yusuf TUTY LASWARDI YUSUF Tuty Laswardi Yusuf Tuty Laswardi Yusuf Tuty Laswardy Yusuf Uus Saefullah Wahono Esthi Prasetyaningtyas Widihasputri, Risti Wilmientje Marlene Mesang Nalley Witri, Brilla Widya Yohana Tri Hastuti Yulida Nofa Yusman Setiawan

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