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Kriopreservasi Semen Domba Garut Menggunakan Tris Kuning Telur yang Disuplementasi Omega-3 Minyak Ikan Salmon (CRYOPRESERVATION GARUT SHEEP SEMEN USING TRIS EGG YOLK SUPPLEMENTED OMEGA-3 FISH OIL SALMON) Nurcholis Nurcholis; Raden Iis Arifiantini; Mohamad Yamin
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The availability of superior seeds garut sheep relatively few and the unavailability of seed supply ofsuperior male garut sheep that is continuously high productive is also a problem. . The success ofcryopreservation is influenced by the diluent being used. This study aimed was to compare the Tris EggYolk containing omega-3 (TEYO) and Tris used commercial egg yolk plus omega-3 fish oil salmon (TEYOS)to the success of cryopreservation of garut sheep semen. Five garut ram aged 1,5 – 2,0 years were used inthis study. Semen was collected using an artificial vagina, then it was evaluated and divided into twotubes, each of them was diluted with TEYO and TEYOS (50x106 sel/straw), before they then packed intostraws, equilibrated (5°C), and frozen with liquid nitrogen vapour (-130oC) for10 minutes and storedthem in the container (-196oC) for further evaluation. The results showed that post thawing values of thesperm motility, viability and intact plasma membrane were 53,75±2,46; 60,75±2,17and 72,58±2,12%respectively, diluted with TEYOS were higher (P<0,05) than those diluted with TEYOS with the spermmotility, viability and intact plasma membrane only at 41,50±1,25; 50,50±1,04 and 57,58±1,03%respectively. This result demonstrated that the recovery rate of spermatozoa in TEYOS diluent washigher (P<0,05) than TEYO. Its concluded that fish salmon oil omega-3 supplemented in TEYOS better incryopreservation of garut ram semen compared to the TEYO diluent.
Sinkronisasi Estrus dan Inseminasi Buatan pada Rusa Timor Wilmientje Marlene Mesang Nalley; Ristika Handarini; Raden Iis Arifiantini; Tuty Laswardi Yusuf; Bambang Purwantara; Gono Semiadi
Jurnal Veteriner Vol 12 No 4 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this experiment was to examine the fertility of timor deer (Rusa timorensis) frozensemen by artificial insemination (AI) with intracervical technique after estrus synchronization withControlled Internal Drug Release, for Goat (CIDR-G®). Six adult, healthy, and cycling hinds aged 3 to 4years were used in this experiment. The percentage of estrus hind, onset and duration of estrus wereobserved. Pregnancy diagnosis was conducted using ultrasound scanner (USG) at day 120 afterinsemination. The response of estrus was 82.22 %, the onset of estrus was 25.33 hours after CIDR®withdrawal with the length of estrus was 28 hours. The pregnancy rate at first AI trial under sedation was16.7% (1/6), at second and third without sedation was 60% (3/5) and 100% (2/2) respectively. The durationof pregnancy was 248 to 285 days; with the average calving rate was 50%. The result concludes thatinsemination in hind without sedation was better than with sedation.
Kualitas Semen Cair Kambing Peranakan Etawah dalam Modifikasi Pengencer Tris dengan Trehalosa dan Rafinosa (THE QUALITY OF ETAWAH CROSSBREED BUCK LIQUID SEMEN IN MODIFIED TRIS DILUENTS WITH TREHALOSE AND RAFFINOSE) Oriza Savitri Ariantie; Tuty Laswardi Yusuf; Dondin Sajuthi; Raden Iis Arifiantini
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study was to compare the efficacy of trehalose and raffinose supplementation in Trisegg yolk (TEY) and Tris soya (TS) diluents in optimizing the quality of Etawah Crossbreed liquid semen.Semen were collected from three sexually mature bucks using artificial vagina. Semen were then evaluatedand divided into six aliquot tubes. Each of them was diluted in TEY and TS supplemented with 50 mMtrehalose or raffinose, respectively. The liquid semen were then stored in refrigerator (5°C). The motility,viability and plasma membrane integrity (PMI) of the spermatozoa were evaluated every 12 hours untilsperm motility remained up to 50%. The results showed that sperm motility in TEY supplemented withtrehalose or raffinose remained up to 50% for 72-84 hours, compared to in TS which was for 48-60 hours(P<0,05). The best diluent was demonstrated by TEY supplemented with trehalose where the spermmotility was 52,82±3,21% up to 84 h compared to raffinose supplementation (52,78±4,41%) and control(51,78±4,86%) which was up to 72 hours, respectively. Meanwhile, the spermatozoa motility in TS diluentsupplemented with raffinose was 52,78±4,41% for up to 60 hours compared to supplemented with trehalose(53,33±3,54%) and control (51,11±4,86%) which was up to 48 hours. In all diluents, the viability ofspermatozoa was 6-9% higher than the percentage of sperm motility, whilst the percentage of PMI wassimilar to the percentage of sperm motility. In conclusion, TEY supplemented with trehalose was the bestdiluents for preservation of Etawah Crossbreed buck liquid semen, and when using TS diluent it isrecommended to add raffinose  rather than trehalose.
Efektivitas Low Density Lipoprotein dan Kuning Telur Ayam dan Puyuh pada Pengawetan Semen Ayam Merawang (EFFECTIVESS OF LOW DENSITY LIPOPROTEIN AND EGG YOLK FROM CHICKEN AND QUAIL ON MERAWANG SEMEN PRESERVATION) Magfira Magfira; Raden Iis Arifiantini; Ni Wayan Karniani Karja; Sri Darwati
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.755 KB) | DOI: 10.19087/jveteriner.2017.18.3.345

Abstract

The successful of artificial insemination (AI) depends on the semen quality and extender. To minimize effect of cold shock during storage, extender is added with egg yolk. The objectives of this study were to compare the effectiveness of pure Low Density Lipoprotein (LDL) and egg yolk from domestic chicken and quail on motility and longevity of Merawang chicken sperm. The semen was collected by massage method from three Merawang roosters. Immediately after collection, semen was evaluated macroscopically and microscopically. Only semen demonstrated >70% motility and <20% sperm abnormality were used in this study. Semen divided into four aliquots and diluted with Lactate Ringer (LR) LDL chicken (RL-LDL-C), LR-LDL quail (LR-LDL-Q), LR- chicken Egg Yolk (LR-CEY), Ringer Lactate quail Egg Yolk (RL-QEY). Diluted semen than stored at 5oC. Sperm motility was examined twice a day and the longevity of sperm was determined every day until the sperm reach 0% motility. The motility of spermatozoa in the LR-LDL diluent differed from the sperm motility in the RL-QEY diluent at the 60th and 72th hour (P <0.05) poststorage. However, there was no difference in motility sperm in LR-LDL-C, RL-LDL-Q and RL-CEY. Additionally, there is no difference (P> 0.05) in spermatozoa longivity in the four diluents, with a range of longivities between 4.43 to 5.93 days. ABSTRAK Keberhasilan inseminasi buatan (IB) salah satunya bergantung pada kualitas semen dan pengencer yang digunakan. Dalam meminimalisir pengaruh cold shock saat penyimpanan, pengencer ditambahkan dengan kuning telur. Tujuan dari penelitian ini adalah untuk membandingkan efektivitas Low Density Lipoprotein (LDL) dan kuning telur yang berasal dari ayam kampung dan puyuh terhadap motilitas dan longivitas spermatozoa ayam. Koleksi semen dilakukan menggunakan metode pemijatan pada tiga ekor ayam merawang. Setelah semen dikoleksi, selanjutnya semen dievaluasi secara makroskopis dan mikroskopis. Semen yang menunjukkan motilitas 70% dan abnormalitas kurang dari 20% dibagi empat dan diencerkan menggunakan Ringer Laktat-LDLA (RL-LDLA), Ringer Laktat-(RL-LDLP), Ringer Laktatkuning telur ayam (RL-KTA), dan RL-kuning telur puyuh (RL-KTP). Semen yang telah diencerkan kemudian disimpan pada suhu 5oC. Motilitas spermatozoa diamati dua kali sehari sampai motilitas mencapai 0%. Motilitas spermatozoa dalam pengencer RL-LDLA berbeda dengan motilitas spermatozoa dalam pengencer RL-KTP pada jam ke-60 dan ke-72 (P<0.05) pascapenyimpanan. Akan tetapi tidak terdapat perbedaan motilitas spermatozoa dalam RL-LDLA, RL-LDLP dan RL-KTA. Longivitas spermatozoa dalam empat pengencer tidak terdapat perbedaan (P>0.05) dengan rentang longivitas antara 4,43 sampai 5,93 hari.
Deteksi Kerusakan DNA Spermatozoa Semen Segar dan Semen Beku Sapi Menggunakan Pewarnaan Toluidine Blue (DETECTION OF SPERM DNA DAMAGE INFRESH AND FROZEN SEMEN USING TOLUIDINE BLUE STAINING) Langgeng Priyanto; Raden Iis Arifiantini; Tuty Laswardi Yusuf
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Sperm DNA integrity is very important in the success of fertilization and embryo development.Toluidine blue (TB) is a sensitive staining to examine the chromatin structure of sperm. The aim of thisstudy was to detect the sperm DNA damage before and after freezing using TB. Semen was obtained fromeight superior bulls (two Brahman, two Ongole, two Simental and two Limosin) belong to Lembang ArtificialInsemination Centre. Semen was collected twice a week using artificial vagina, then was evaluatedmacrocopically and microscopically after collected, including sperm motility, viability, plasma membraneintegrity (MI) and acrosome intact (AI), sperm concentration, abnormality and sperm DNA integrity. Thesemen that been used in this study showed the total motility was more than 70%, sperm concentrationwas more than 1000x106, and the sperm abnormality was below 20%. The result showed that the qualityof semen after freezing processed was decreased significantly (P<0.05) on percentage of sperm motility,viability, MI and AI, whereas there was no different on DNA integrity (P>0.05). Sperm DNA integrity offresh and frozen semen were 93.91±4.77% and 92.06 ±2.41% respectively. The decrease of DNA integritywas low (1.84%) compared to motility (28.3%), viability (21.6%), MI (14.1%), and AI (11.8%). In conclusion,toluidine blue can be used to detect of DNA damage and the freezing process will not decrease the DNAintegrity.
Korelasi Antara Lama Kebuntingan, Bobot Lahir dan Jenis Kelamin Pedet Hasil Inseminasi Buatan pada Sapi Bali Gatot Prasojo; Iis Arifiantini; Kusdiantoro Mohamad
Jurnal Veteriner Vol 11 No 1 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Purpose of the research was to study the correlation between the gestation period with sex and birthweight of new born calves as the result of artificial insemination (AI) in bali cattle. Data were collecteddirectly from AI technicians in some districts in the province of bali from 1997 to 2003. A total, of 10759inseminated cows and 799 of which were with completed data of, date of birth, birth weight and sex ofcalves. The data were collected from 3 districts, involving Gianyar (205 samples), Badung (221 samples)and Bangli (373 samples). Result showed that the gestation period of bali cows were 284.4±5.7 days andbirth weight of 18.4±1.6 kg. Sex ratios (male : female) of bali calves resulted from AI in 1997 to 2003 were1.2:1. There were positive correlation between gestation period and birth weight (P>0.01) as well asbetween gestation period and sex of calves with a high correlation coefficient (0.075) which proved thatmale calves had longer gestation period than females.
Hubungan antara Konsentrasi Testosteron, Lingkar Skrotum, Libido dan Kuantitas Sperma Pejantan Sapi Pasundan Santoso Santoso; Herdis Herdis; Raden Iis Arifiantini; Asep Gunawan; Cece Sumantri
Jurnal Veteriner Vol 22 No 3 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (108.864 KB) | DOI: 10.19087/jveteriner.2021.22.3.389

Abstract

Sapi pasundan merupakan salah satu sumber daya genetik sapi potong lokal Indonesia yang perlu dikembangkan dan dilestarikan. Penelitian bertujuan untuk menganalisis hubungan konsentrasi testosteron, lingkar skrotum, libido, dan kuantitas sperma pada sapi pejantan pasundan. Sepuluh ekor sapi pasundan berumur 3-6 tahun dengan bobot badan (BB) 380-430 kg digunakan pada penelitian. Konsentrasi hormon testosteron dianalisa dengan metode enzyme-linked immunosorbent assay (ELISA). Libido diamati dari waktu pejantan didekatkan ke kandang atau tempat koleksi semen hingga kontak pertama dengan betina (courtship), waktu pejantan melakukan percumbuan (dating) sampai menaiki betina (mounting), waktu pejantan dating dan pertama kali menaiki betina sampai terjadi ejakulasi. Pengukuran lingkar skrotum menggunakan pita ukur. Semen dikoleksi menggunakan vagina buatan dan dievaluasi karakteristik volume semen dan konsentrasi sperma. Data dianalisa dengan korelasi regresi linear. Rataan konsentrasi testosteron adalah 13,38±0,21 ng/mL. Konsentrasi testosteron (r = -0,661) dan umur (r = 0,681) menunjukkan korelasi negatif dengan waktu courtship (P<0,05), waktu mounting berkorelasi positif dangan waktu ejakulasi (r = 0,756, P <0,05). Korelasi tidak ditemukan antara konsentrasi testosteron dengan umur (r = 0.116), lingkar skrotum (r = 0.328), peningkatan (r = 0.249), ejakulasi (r = -0.179), volume semen (r = 0.243), dan konsentrasi sperma (r = -0.116). Penelitian menyimpulkan konsentrasi testosteron dan umur berkorelasi negatif dengan courtship dan waktu mounting berkorelasi positif dangan waktu ejakulasi.
Identifikasi Bakteri dan Efektivitas Antibiotik dalam Pengencer untuk Mengontrol Pertumbuhan Bakteri pada Semen Sapi Friesian Holstein Muttaqinullah Rabusin; Andriani Andriani; Raden Iis Arifiantini; Ni Wayan Kurniani Karja
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (125.835 KB) | DOI: 10.19087/jveteriner.2019.20.1.140

Abstract

This study was designed to investigate the presence of bacterial species in Friesian Holstein (FH) bovine semen at the time of collection, processing and to assess the efficacy of two types of antibiotics combinations; penicillin and streptomycin (PS) and gentamycin, tylosin, lincomycin and spectinomycin (GTLS) in semen extender on bacterial control and quality of semen. For this purpose, three experiments were conducted. In experiment 1, identification of bacterial content in fresh semen which collected from 5 bovine ejaculates. In experiment 2, identification of bacterial content in skimmilk-eggyolk extender which were prepared in artificial insemination center, Lembang, Bandung. In experiment 3, identification of bacterial content in frozen thawed semen. In the result, some of bacterial species were isolated from the bovine semen. The GTLS combination of antibiotics may be incorporated into a freezing extender or protocol without compromising the post-thawed semen quality of FH bull spermatozoa. Three types of bacteria were found in fresh semen; Klebsiella sp., Micrococcus sp., and Pediococcus sp.. Three types of bacteria were found in semen extender; Enterobacter cloacae, Pseudomonas diminuta and Serratia plymutica. Two types of bacteria were found in frozen semen; Enterobacter cloacae and Serratia plymutica. In conclusion, antibiotics PS and GTLS were effective for controlling the growth of bacteria in frozen semen.
Penggunaan Dimethyl Sulfoxide Sebagai Krioprotektan dalam Pembekuan Semen Ayam Kampung (THE USE OF DIMETHYL SULFOXIDE AS CRYOPROTECTIVE AGENT FOR NATIVE CHIKEN FROZEN SEMEN) Junaedi Junaedi; Raden Iis Arifiantini; Cece Sumantri; Asep Gunawan
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study aim was to determine the best dimethyl sulfoxide (DMSO) concentration for native chicken’s(Gallus gallus domesticus) frozen semen. Semen was collected from three native chickens and then evaluatedmacroscopically and microscopically. Spermatozoa that used in this study had more than 75% spermmotility and more than 25 X 108 mL in sperm concentration. Semen was divided into three equal tubesand each of them diluted with Ringer lactate egg yolk (RLEY) extender consisted of 5, 7 and 9% of DMSO.Diluted semen was added into 0.25 mL of mini straw, then equilibrated at 5 °C for two hours. The semenwas then frozen placed in liquid nitrogen vapors for10 min. Finally, the semen was put into liquid nitrogenwith a temperature of – 196oC. Post thawing quality semen was assessed after 24 hours storage bythawing the straw in warm water (37 °C) for 30 seconds. Data were analyzed using one-way analysis ofvariance. The results demonstrated that the sperm motility and viability diluted with RLEY extenderwith 7% DMSO was better (p<0.05) than those in 5 or 9% DMSO. Semen quality decreased at every stepof freezing, especially between equilibration and thawing. Its concluded that 7% was the best DMSOconcentration for freezing of native chicken semen in Ringer lactate egg yolk extender compared to 5% and9%.
Kualitas Semen Beku Babi dalam Pengencer Komersial yang Disuplementasi dengan Trehalosa (THE QUALITY OF BOAR FROZEN SEMEN IN COMMERCIAL EXTENDER SUPPLEMENTED WITH TREHALOSE) Tuty Laswardi Yusuf; Raden Iis Arifiantini; Reni Ratni Dapawole; Wilmientje Marlene Mesang Nalley
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (105.27 KB) | DOI: 10.19087/jveteriner.2017.18.1.69

Abstract

The aim of the research was to study trehalosa supplementation in Beltsville Thawing Solution (BTS) and MIII extender on the quality of frozen boar semen. Semen was collected from nine boars age 5-6 years using glove hand method. The semen were then evaluated macro and microscopically. Semen having >70% sperm motility and >200x106/mL sperm concentration were diluted in two tubes containing BTS and two tubes containing MIII, then kept at room temperature (20-22oC) for 2 hours (holding time). Following this the semen were centrifugated at 2000 RPM for 10 minutes and the supernatant was discarded, the pellet was re-diluted with BTS, BTS-Trehalose 100 mM (BTS-T), MIII, and MIII-Trehalose 100 mM (MIII-T) with 4% glycerol, packed into 5 mL macrotube, equilibrate at 5oC for 2 hours. After equilibration macrotube were frozen horizontally 4 cm above the liquid nitrogen vapor for 20 minutes and then submerged in liquid nitrogen (-196oC) for futher evaluation. After 24 hours the semen than thawed at 52oC for 45 seconds. The results demonstrated that sperm motility in BTS-T (30.00±4.47%) was higher (P<0.05) then MIII-T (26.67±8.16%), BTS (24.17±9.17%), or MIII (21.67±7.53%). This research conclude that suplementation of 100 mM trehalosein BTS extender improved motility of boar sperm after freezing and thawing. ABSTRAK Penelitian ini bertujuan untuk mempelajari penambahan trehalosa dalam pengencer Beltsville Thawing Solution (BTS) dan MIII terhadap kualitas semen beku babi. Semen dikoleksi dari sembilan ekor babi berumur 5-6 tahun menggunakan teknik glove hand method. Semen dievaluasi secara makro dan mikroskopis. Semen yang memiliki spermatozoa motil di atas 70% dengan konsentrasi di atas 200x106/mL dibagi ke dalam empat tabung, dua tabung pertama diencerkan dengan BTS dan dua tabung lainnya dengan MIII. Semen yang telah diencerkan disimpan pada suhu ruangan (20-22oC) selama dua jam (holding time). Semen kemudian disentrifugasi 2000 rpm selama 10 menit. Supernatan dibuang dan pellet diencerkan kembali. Semen yang disentrifugasi dengan BTS, diencerkan dengan BTS gliserol (BTSg) dan BTS gliserol Trehalosa 100 mM (BTSgT) dan yang disentrifugasi dengan MIII diencerkan dengan MIIIg dan MIIIgT. Gliserol yang digunakan adalah 4%. Semen yang telah diencerkan dikemas dalam macrotube (5 mL), diekuilibrasi pada 5oC selama dua jam. Setelah equilibrasi macrotube dibekukan 4 cm di atas permukaan nitrogen cair selama 20 menit dan disimpan dalam container nitrogen cair (-196oC) untuk pengujian lebih lanjut. Setelah penyimpanan 24 jam, terhadap semen beku dilakukan thawing pada suhu 52oC selama 45 detik. Hasil penelitian menunjukkan motilitas spermatozoa dalam BTSgT (30,00±4,47%) paling tinggi (p<0,05) daripada MIIIgT (26,67±8,16%), BTSg (24,17±9,17%), atau MIIIg (21,67±7,53%). Simpulan dari penelitian ini adalah penambahan 100 mM trehalosa dalam pengencer BTSg dapat mempertahankan motilitas spermatozoa babi lebih baik daripada pengencer lainnya.
Co-Authors . Soeparna A.A. Ketut Agung Cahyawan W Abdullah Baharum Abdullah Baharun Agasi, Satri Yusasra Amani, Aqila Zata Aminudin Aminudin Anak Agung Gede Sugianthara Andriani Andriani Angelina N. Tethool Anna Nabilla Anny Amaliya Asep Gunawan Asep Kurnia Asep Kurnia Azizah - Baharum, Abdullah Bambang Purwantara Bq Hayyul Hidayati Cece Sumantri Damiana Rita Ekastuti Dedi Rahmat Setiadi DEWI APRI ASTUTI Diana Andrianita Kusumaningrum Dondin Sajuthi Eko Prasetyo Nugroho Elmanaviean, Muhammad Emilia Kamung Hambu, Emilia Kamung Enniek Herwiyanti Faisal Amri Satrio Frederika Katerina Foeh, Nancy Diana Gatot Prasojo GONO SEMIADI Gunawan Sitanggang Gusdinar, Rizal Hasbi Hasbi Hastuti, Yohana Tri Henny Nuraini herdis herdis Herry Sonjaya Hikmayani Iskandar Imam Purwadi, Imam Iman Supriatna Indriastuti, Rhesti Jakaria Jakaria Jansen Manansang Jansen Manansang Jean-Baptise Menassol Junaedi Junaedi Junaedi Junaedi Ketut Adnyane Mudite Khaeruddin Khaeruddin Khaeruddin Khaeruddin Komariah Komariah Kurnia Bagus Ariyanto Kusdiantoro Mohamad Laksono, Mohamad Nanang Tejo Langgeng Priyanto Lilis Khotijah Magfira Magfira Magfira Magfira Magfira Maghfira Maghfira Manansang, Willem Memili, Erdogan Mohamad Yamin Mohamad Yamin Mohamad Yamin Mokhamad Fahrudin MUHAMMAD AGIL Muhammad Agus Tahar Muhammad Gunawan Mulia, Bongot Huaso Muttaqinullah Rabusin Nancy D. F. K. Foeh Ni Wayan Karniani Karja Ni Wayan Kurniani Karja Niken Ulupi Nila Pratiwi Nugraha, Arifin Budiman Nur Hidayati Nurcholis Nurcholis Nurcholis Nurcholis Nurcholis Nurcholis Nuri Andarwulan Oriza Savitri Ariantie Palupi, Nurheni Sri Pardede , Berlin Pandapotan Pardede, Berlin Pandapotan Prayetno, Saptohadi Puwantara, Bambang Rahmat Hidayat Rahmat Hidayat Reni Ratni Dapawole Rhesti Indriastuti Rhomadon, Gilang Ria Ariyanti Ristika Handarini Riwu, Ronny Markus Jami Rudi Afnan Santoso Santoso Setyaningsih Rambu Liwa Siti Zubaidah Soeparna Soeparna Sri Darwati Sri Darwati Srihadi Agungpriyono Syahruddin Said Teguh Ari Prabowo Tejolaksono, Muhammad Nanang Tike Sartika Tike Sartika Triansyah, Irvan Tulus Maulana Tuti Suryati Tuty Laswardi Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf TUTY LASWARDI YUSUF Tuty Laswardi Yusuf TUTY LASWARDI YUSUF Tuty Laswardi Yusuf Tuty Laswardy Yusuf Uus Saefullah Wahono Esthi Prasetyaningtyas Widihasputri, Risti Wilmientje Marlene Mesang Nalley Witri, Brilla Widya Yohana Tri Hastuti Yulida Nofa Yusman Setiawan