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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Buletin Peternakan ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Ilmu Ternak Veteriner AGRICOLA Animal Production : Indonesian Journal of Animal Production Biosaintifika: Journal of Biology & Biology Education Jurnal Medik Veteriner Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) ARSHI Veterinary Letters JURNAL KAJIAN VETERINER Indonesian Journal of Applied Research (IJAR) Jurnal Nukleus Peternakan Jurnal Kedokteran Hewan Chalaza Journal of Animal Husbandry Policy Brief Pertanian, Kelautan, dan Biosains Tropika Jurnal Agripet Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia Biodiversitas Journal of Biological Diversity
R. Iis Arifiantini
IPB University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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EFFECTIVENESS OF VARIOUS COMMERCIAL EXTENDERS IN CRYOPRESERVING SEMEN OF LOCAL INDONESIAN RAMS Setiadi, Dedi Rahmat; Magfira; Riwu, Ronny Markus Jami; Arifiantini, R Iis
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.9208

Abstract

Indonesian local sheep represent a particular species of sheep reared by farmers on small-scale farms. Semen freezing can be performed using either a homemade or commercial extender. The present study evaluated the effectiveness of several commercial extenders in freezing Indonesian local sheep. Semen was collected from three mature and healthy rams using an artificial vagina and evaluated. It was determined that only samples exhibiting sperm motility levels of greater than 70% would be included in the study. Subsequently, the semen from each ram was divided into three portions and diluted with Andromed, Sterydil, and OptiXcell, respectively. The frozen semen was then evaluated 24 hours post-freezing. The thawing process was conducted individually at 37°C for 30 seconds. The evaluation encompassed a range of metrics, including sperm motility, viability, and the presence of any abnormalities, in addition to the recovery rate. The results indicated that sperm frozen in Optixcell and Sterydil extenders exhibited superior motility, viability, and recovery rate than Andromed. The study also demonstrated no interaction between individual Ram and extender, and no differences were found between individuals. The study concluded that Optixcell and Sterydil extenders effectively froze Indonesian local sheep semen.
FROZEN SEMEN CHARACTERISTICS OF BALI BULL IN DIFFERENT AGE GROUPS Witri, Brilla Widya; Arifiantini, Iis; Prasetyaningtyas, Wahono Esthi; Karja, Ni Wayan Kurniani
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10087

Abstract

One factor that affects frozen semen characteristics is bull age. Increasing age induces changes in sperm that reduce frozen semen quality. This study aimed to evaluate the frozen semen characteristics of Bali bulls aged 3, 8, and 13 years. Frozen semen was derived from fresh semen with sperm motility > 70%. The frozen semen characteristics tested included motility, viability, plasma membrane integrity, acrosome integrity, and sperm abnormalities. Sperm motility was analyzed by computer-assisted sperm analysis (CASA). Viability was assessed using eosin-nigrosin staining; plasma membrane integrity was assessed using the hypoosmotic swelling test (HOST); acrosome integrity was assessed using Giemsa staining; and sperm abnormalities were assessed using Williams staining. The plasma membrane integrity of the 13-year-old group was significantly lower (P < 0.05) than that of the younger age groups. However, there were no significant differences (P > 0.05) in the other parameters. We conclude that age affects the integrity of the sperm plasma membrane in frozen semen from Bali bulls.
Longevity and Recovery Rate of Indonesian Local Ram Spermatozoa in Homemade and Commercial Extenders Kamal, Lutfhi Annisa; Agil, Muhammad; Said, Syahruddin; Nugraha, Taufiq Purna; Arifiantini, Raden Iis
Indonesian Journal of Applied Research (IJAR) Vol. 7 No. 1 (2026): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v7i1.827

Abstract

Sheep farming plays a crucial role in Indonesia's livestock industry; however, the effectiveness of artificial insemination (AI) is hindered by the less-than-ideal quality of cryopreserved semen. This research assessed the longevity and progressive motility recovery rate (RR) of frozen semen from local Indonesian rams, using a homemade Tris egg yolk (TEY) extender and three commercial extenders (AndroMed®, Steridyl®, BoviFree®). Semen from three rams (Batur, Garut, and Sakub) was processed, frozen in 0.25 mL straws (50 × 10⁶ sperm per straw), and its quality was evaluated using CASA. Longevity was monitored hourly from 0 to 4 h at 37°C. The data were analyzed using a Randomized Block Design with ANOVA, followed by Duncan’s Multiple Range Test. Sperm motility decreased over incubation time for all extenders, with notable differences at 4 h (total motility, p = 0.017; progressive motility, p = 0.023). Commercial extenders preserved higher total and progressive motility than TEY, with Steridyl® showing the highest mean RR (64.11%; mean all rams; RR analysis, n = 18), significantly outperforming Andromed®, BoviFree®, and TEY (p = 0.007). The superior results of Steridyl® align with its low-density lipoprotein content, which stabilizes the sperm membranes during cryopreservation. In summary, Steridyl® was the most effective extender for maintaining motility and RR of frozen semen from local Indonesian rams, potentially enhancing AI success in small ruminant programs.
SPERM LONGEVITY AND RECOVERY RATE OF BELGIAN BLUE CATTLE IN DIFFERENT EXTENDER Andi Murni Nurul Maulidyah; Muhammad Agil; Syahruddin Said; Taufiq Purna Nugraha; Raden Iis Arifiantini
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Frozen semen from Belgian Blue cattle is in high demand among farmers, as evidenced by the increasing interest in this breed's semen. However, the freezing process reduces sperm motility and viability. This study aimed to assess the viability of sperm post-freezing and the sperm recovery rate (RR) in Belgian Blue cattle using different extenders. Semen was collected, evaluated, and diluted in four extenders: three commercially available ones—BoviFree®, AndroMed®,, and Steridyl®,—and a homemade extender, Tris Egg Yolk (TEY). Longevity and RR tests were conducted 24 h after freezing. Progressive motility was assessed using a portable computer-assisted sperm analysis (CASA) device (Androscope Minitube, Germany). The results indicated that sperm motility was higher in the BoviFree®, and AndroMed®, extenders after 3 h compared to the other extenders (p <0.05). After 4 h, motility remained higher in the BoviFree®, extender than in TEY. The RR values showed no significant differences among the extenders (p< 0.05). The study concluded that sperm longevity in Belgian Blue cattle was optimal in the BoviFree®, extender, with consistently good sperm RR values across all extenders.
SPERM LONGEVITY AND RECOVERY RATE OF BELGIAN BLUE CATTLE IN DIFFERENT EXTENDER Andi Murni Nurul Maulidyah; Muhammad Agil; Syahruddin Said; Taufiq Purna Nugraha; Raden Iis Arifiantini
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Frozen semen from Belgian Blue cattle is in high demand among farmers, as evidenced by the increasing interest in this breed's semen. However, the freezing process reduces sperm motility and viability. This study aimed to assess the viability of sperm post-freezing and the sperm recovery rate (RR) in Belgian Blue cattle using different extenders. Semen was collected, evaluated, and diluted in four extenders: three commercially available ones—BoviFree®, AndroMed®,, and Steridyl®,—and a homemade extender, Tris Egg Yolk (TEY). Longevity and RR tests were conducted 24 h after freezing. Progressive motility was assessed using a portable computer-assisted sperm analysis (CASA) device (Androscope Minitube, Germany). The results indicated that sperm motility was higher in the BoviFree®, and AndroMed®, extenders after 3 h compared to the other extenders (p <0.05). After 4 h, motility remained higher in the BoviFree®, extender than in TEY. The RR values showed no significant differences among the extenders (p< 0.05). The study concluded that sperm longevity in Belgian Blue cattle was optimal in the BoviFree®, extender, with consistently good sperm RR values across all extenders.
THE EVALUATION OF FROZEN SEMEN PRODUCTION POTENTIAL IN ETAWAH GRADE AND ANGLO-NUBIAN BREEDS (Evaluasi Potensi Produksi Semen Beku pada Kambing Peranakan Etawah dan Anglo Nubian) Casmuti Casmuti; Mokhamad Fakhrul Ulum; Syahruddin Said; Taufiq Purna Nugraha; Asep Kurnia; Raden Iis Arifiantini
JURNAL NUKLEUS PETERNAKAN Vol 13 No 1 (2026): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v13i1.27812

Abstract

This study aimed to evaluate the potential of frozen semen production in Etawah Grade and Anglo-Nubian breeds based on fresh semen characteristics and to assess variations among individuals and breeds. The research was conducted at the Lembang Artificial Insemination Center, West Bandung, using primary data from five ejaculations per individual and secondary data from the year 2024. Fresh semen was evaluated for semen volume, sperm motility, and sperm concentration, reflecting the number of straws that can be produced per ejaculation. Frozen semen production potential was calculated based on semen volume, sperm motility, and sperm concentration. The results showed significant differences (P<0.01) in production potential among individuals and between breeds. The highest production potential was observed in the buck named Andromeda (122.45 ± 2.07 straws), which was significantly higher than that of other individuals. Anglo-Nubian breed showed a higher production potential (105.76 ± 2.54 straws) compared to Etawah Grade (91.63 ± 1.92 straws). However, Etawah Grade bucks exhibited relatively better sperm motility. In conclusion, both individual and breed factors significantly influence frozen semen production potential. Anglo-Nubian bucks have higher production capacity, while Etawah Grade bucks may offer better sperm quality. These findings provide important insights for optimizing breeding strategies and semen production planning in artificial insemination programs.
Pengembangan Uji Berbasis Molekuler Protamine-1 (Prm1) Sebagai Penentu Tingkat Fertilitas untuk Proses Seleksi dan Kebijakan Pengafkiran Sapi Pejantan Unggul di Indonesia Bambang Purwantara; Berlin Pandapotan Pardede; Muhammad Agil; Iman Supriatna; Ni Wayan Kurniani Karja; R. Iis Arifiantini; Cece Sumantri
Policy Brief Pertanian, Kelautan, dan Biosains Tropika Vol. 4 No. 4 (2022): Policy Brief Pertanian, Kelautan dan Biosains Tropika
Publisher : Direktorat Kajian Strategis dan Reputasi Akademik IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agro-maritim.0404.367-374

Abstract

Standar kualitas semen yang berperan penting dalam keberhasilan inseminasi buatan (IB) sebagai upaya peningkatan populasi dan mutu ternak sapi di Indonesia terbukti tidak seutuhnya mampu menentukan tingkat fertilitas sapi pejantan di Indonesia. Studi terbaru membuktikan bahwa meskipun sudah memenuhi standar kualitas semen yang sudah ditetapkan, namun tiga dari enam ekor sapi pejantan di Indonesia masih tergolong fertilitas rendah (<60%). Potamine-1 (PRM1) merupakan molekul pada inti spermatozoa yang telah terbukti memiliki hubungan erat dengan fungsi fertilitas dan kualitas spermatozoa pada berbagai sapi pejantan di Indonesia. Hal tersebut mendasari usulan pengembangan uji berbasis molekuler PRM1 sebagai penentu tingkat fertilitas yang diharapkan dapat digunakan untuk proses seleksi dan kebijakan pengafkiran sapi pejantan unggul di Indonesia. Melalui policy brief ini diharapkan usulan pengembangan uji tersebut dapat lebih tersampaikan dengan segala solusi yang diberikan, termasuk terkait pendekatan uji berbasis molekuler yang paling tepat digunakan, kajian-kajian lanjutan, dan berbagai harapan dukungan dari segala pihak demi terwujudnya pengembangan uji PRM1.
Short Communication: Characteristics of the endangered Javan banteng (Bos javanicus) spermatozoa Vincentia Trisna Yoelinda; Raden Iis Arifiantini; Dedy Duryadi Solihin; Muhammad Agil; Dedi Rahmat Setiadi; Yohana Tri Hastuti; Jansen Manansang; Dondin Sajuthi
Biodiversitas Journal of Biological Diversity Vol. 24 No. 2 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240211

Abstract

Abstract. Yoelinda VT, Arifiantini RI, Solihin DD, Agil M, Setiadi DR, Hastuti YT, Manansang J, Sajuthi D. 2023. Short Communication: Characteristics of the endangered Javan banteng (Bos javanicus) spermatozoa. Biodiversitas 24: 759-766. Morphology and morphometry of spermatozoa become essential fertility parameters in domestic and wild animals. Such morphological abnormalities can arise from genetic or environmental origins and cause fertility deprivation. Semen collection using transrectal massage has been used in several domestic and wild animals and is known to be simpler, safer, and less painful than artificial vaginas and electro-ejaculation. This study aimed to evaluate morphological and morphometrical features of the banteng spermatozoa head. The objective of this study was also, to evaluate the use of transrectal massage as a semen collection protocol. This study was conducted in Taman Safari Indonesia, Cisarua, Bogor, West Java, involving one banteng (Bos javanicus) bull. Semen samples were collected weekly, smeared, and stained with a modified Williams' staining method. This study found ten types of head morphological abnormality, comprising several major defects, with several types known to be the genetic origin. There were also nine types of spermatozoa tail abnormalities and teratoid form abnormalities. The total normal spermatozoa proportion in this study was 54.30±1.74%. Morphometric dimensions of length, width, and area head of banteng spermatozoa were 9.88±0.01 µm, 4.91 µm, and 38.12±0.05 µm2, respectively, with a tail length of 60.48±0.03 ?m. Although our result was limited by the number of samples available for the study, this first report on the banteng bull's sperm morphology and morphometry still need to be considered. The possibility of transrectal massage use for banteng semen collection protocol demonstrated in this study may be helpful to support the conservation approach in terms of breeding program strategy.
Meningkatkan angka kehamilan pada sapi Bali (Bos javanicus) melalui sinkronisasi estrus berbasis prostaglandin F₂α Dedi Rahmat Setiadi; Yohana Tri Hastuti; Bongot Huaso Mulia; Imam Purwadi; Muhammad Nanang Tejolaksono; Gilang Rhomadon; Risti Widihasputri; Saptohadi Prayetno; Jansen Manansang; Raden Iis Arifiantini
ARSHI Veterinary Letters Vol. 9 No. 4 (2025): ARSHI Veterinary Letters - November 2025
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.9.4.115-116

Abstract

Penelitian ini mengevaluasi efektivitas sinkronisasi estrus menggunakan prostaglandin F2α (PGF2α) pada sapi Bali dan menilai hasil kehamilan setelah perlakuan awal dan resinkronisasi selanjutnya. Sepuluh sapi Bali menerima dua suntikan PGF2α, diberikan dengan jarak 11 hari, sesuai rekomendasi standar. Hewan yang menunjukkan estrus diinseminasi buatan, dan diagnosis kehamilan dilakukan 30–45 hari setelah inseminasi menggunakan ultrasonografi. Sapi yang gagal hamil selama siklus pertama menjalani protokol resinkronisasi dua minggu kemudian. Protokol sinkronisasi menghasilkan respons estrus 100%, dengan tingkat kehamilan awal 40% (4 sapi). Resinkronisasi enam sapi yang tidak hamil menghasilkan dua kehamilan tambahan (33,3%), sehingga menghasilkan tingkat kehamilan keseluruhan 60%. Temuan ini menunjukkan bahwa PGF2α efektif dalam mengatur siklus estrus dan meningkatkan efisiensi reproduksi sapi Bali, terutama bila diintegrasikan dengan strategi resinkronisasi.
COMPARATIVE ASSESSMENT OF BULL SPERM MOTILITY AND CONCENTRATION: CONVENTIONAL METHODS VERSUS PORTABLE ANDROSCOPE CASA SYSTEM Raden Iis Arifiantini; Khalis Talitha Gusman; Muhammad Agil; Ni Wayan Kurniani Karja
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10400

Abstract

Operational implementation, such as laboratory staff and equipment, differ for each frozen semen producer, resulting in variability in the quality assessment of the frozen semen produced. Conventional assessments subject to subjectivity, human error, and high variability. Computer-assisted semen analysis (CASA) is considered more objective. This study aimed was to conduct a comparative analysis of motility and concentration assessment of frozen semen sperm using conventional and the portable CASA AndroScope. Twenty-one laboratory assistants from 21 national and regional frozen semen producers participated in this study. Sperm motility and concentration were assessed conventionally (using a Neubauer chamber) and AndroScope was used with five replicates. The results of the comparison of sperm motility scores revealed significant differences (p<0.05) between conventional examination and using AndroScope. The results of the comparison of the sperm concentration calculations revealed no significant difference (p>0.05) between the calculations performed using the Neubauer chamber and AndroScope. Assessment with the AndroScope is considered reliable and can replace conventional assessment in assessing motility and sperm concentration, thereby improving and standardizing the assessment performed by frozen semen producers.
Co-Authors . Soeparna A.A. Ketut Agung Cahyawan W Abdullah Baharum Abdullah Baharun Agasi, Satri Yusasra Amani, Aqila Zata Aminudin Aminudin Anak Agung Gede Sugianthara Andi Murni Nurul Maulidyah Andriani Andriani Angelina N. Tethool Anna Nabilla Anny Amaliya Asep Gunawan Asep Kurnia Asep Kurnia Asep Kurnia Azizah - Baharum, Abdullah Bambang Purwantara Berlin Pandapotan Pardede Bongot Huaso Mulia Bq Hayyul Hidayati Casmuti Casmuti Cece Sumantri Damiana Rita Ekastuti Darwati, Sri Dedi Rahmat Setiadi Dedy Duryadi Solihin DEWI APRI ASTUTI Diana Andrianita Kusumaningrum Dondin Sajuthi Dondin Sajuthi Elmanaviean, Muhammad Emilia Kamung Hambu, Emilia Kamung Enniek Herwiyanti Faisal Amri Satrio Frederika Katerina Foeh, Nancy Diana Gatot Prasojo Gilang Rhomadon GONO SEMIADI Gunawan Sitanggang Gusdinar, Rizal Hasbi Hasbi Hastuti, Yohana Tri Henny Nuraini herdis herdis Herry Sonjaya Hikmayani Iskandar Imam Purwadi Iman Supriatna Indriastuti, Rhesti Jakaria Jakaria Jansen Manansang Jansen Manansang Jansen Manansang Jansen Manansang Jean-Baptise Menassol Junaedi Junaedi Junaedi Junaedi Kamal, Lutfhi Annisa Ketut Adnyane Mudite Khaeruddin Khaeruddin Khaeruddin Khalis Talitha Gusman Komariah Komariah Kurnia Bagus Ariyanto Kusdiantoro Mohamad Laksono, Mohamad Nanang Tejo Langgeng Priyanto, Langgeng Lilis Khotijah Magfira Magfira Magfira Magfira Magfira Maghfira Maghfira Manansang, Willem Memili, Erdogan Mohamad Yamin Mohamad Yamin Mohamad Yamin Mokhamad Fahrudin Mokhamad Fakhrul Ulum, Mokhamad Muhammad Agil Muhammad Agil Muhammad Agil Muhammad Agil Muhammad Agus Tahar Muhammad Gunawan Muhammad Nanang Tejolaksono Mulia, Bongot Huaso Muttaqinullah Rabusin Nancy D. F. K. Foeh Ni Wayan Karniani Karja Ni Wayan Kurniani Karja Ni Wayan Kurniani Karja Niken Ulupi NILA PRATIWI Nugraha, Arifin Budiman Nur Hidayati Nurcholis Nurcholis Nurcholis Nurcholis Nurcholis Nurcholis Nuri Andarwulan Oriza Savitri Ariantie Palupi, Nurheni Sri Pardede , Berlin Pandapotan Puwantara, Bambang Rahmat Hidayat Rahmat Hidayat Reni Ratni Dapawole Rhesti Indriastuti Ria Ariyanti Risti Widihasputri Ristika Handarini Riwu, Ronny Markus Jami Rudi Afnan Santoso Santoso Saptohadi Prayetno Setyaningsih Rambu Liwa Siti Zubaidah Soeparna Soeparna Sri Darwati Srihadi Agungpriyono Syahruddin Said Syahruddin Said Syahruddin Said Syahruddin Said Taufiq Purna Nugraha Taufiq Purna Nugraha Taufiq Purna Nugraha, Taufiq Purna Teguh Ari Prabowo Tike Sartika Tike Sartika Triansyah, Irvan Tulus Maulana Tuti Suryati, Tuti Tuty Laswardi Yusuf TUTY LASWARDI YUSUF Tuty Laswardi Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf TUTY LASWARDI YUSUF Tuty Laswardy Yusuf Uus Saefullah Vincentia Trisna Yoelinda Wahono Esthi Prasetyaningtyas Wilmientje Marlene Mesang Nalley Witri, Brilla Widya Yohana Tri Hastuti Yohana Tri Hastuti Yohana Tri Hastuti Yulida Nofa Yusman Setiawan