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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Ilmu Ternak Veteriner AGRICOLA Animal Production : Indonesian Journal of Animal Production Biosaintifika: Journal of Biology & Biology Education Jurnal Medik Veteriner Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) ARSHI Veterinary Letters JURNAL KAJIAN VETERINER Indonesian Journal of Applied Research (IJAR) Jurnal Kedokteran Hewan Chalaza Journal of Animal Husbandry Policy Brief Pertanian, Kelautan, dan Biosains Tropika Jurnal Agripet Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
R. Iis Arifiantini
Divisi Reproduksi Dan Kebidanan, Departemen Klinik, Reproduksi, Dan Patologi, Fakultas Kedokteran Hewan, IPB, Jl Agatis, Kampus IPB, Dramaga, Bogor, Jawa Barat, Indonesia,16880.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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COMPARATIVE ASSESSMENT OF BULL SPERM MOTILITY AND CONCENTRATION: CONVENTIONAL METHODS VERSUS PORTABLE ANDROSCOPE CASA SYSTEM Arifiantini, Raden Iis; Gusman, Khalis Talitha; Agil, Muhammad; Karja, Ni Wayan Kurniani
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10400

Abstract

Operational implementation, such as laboratory staff and equipment, differ for each frozen semen producer, resulting in variability in the quality assessment of the frozen semen produced. Conventional assessments subject to subjectivity, human error, and high variability. Computer-assisted semen analysis (CASA) is considered more objective. This study aimed was to conduct a comparative analysis of motility and concentration assessment of frozen semen sperm using conventional and the portable CASA AndroScope. Twenty-one laboratory assistants from 21 national and regional frozen semen producers participated in this study. Sperm motility and concentration were assessed conventionally (using a Neubauer chamber) and AndroScope was used with five replicates. The results of the comparison of sperm motility scores revealed significant differences (p<0.05) between conventional examination and using AndroScope. The results of the comparison of the sperm concentration calculations revealed no significant difference (p>0.05) between the calculations performed using the Neubauer chamber and AndroScope. Assessment with the AndroScope is considered reliable and can replace conventional assessment in assessing motility and sperm concentration, thereby improving and standardizing the assessment performed by frozen semen producers.
COMPARATIVE ASSESSMENT OF BULL SPERM MOTILITY AND CONCENTRATION: CONVENTIONAL METHODS VERSUS PORTABLE ANDROSCOPE CASA SYSTEM Arifiantini, Raden Iis; Gusman, Khalis Talitha; Agil, Muhammad; Karja, Ni Wayan Kurniani
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10400

Abstract

Operational implementation, such as laboratory staff and equipment, differ for each frozen semen producer, resulting in variability in the quality assessment of the frozen semen produced. Conventional assessments subject to subjectivity, human error, and high variability. Computer-assisted semen analysis (CASA) is considered more objective. This study aimed was to conduct a comparative analysis of motility and concentration assessment of frozen semen sperm using conventional and the portable CASA AndroScope. Twenty-one laboratory assistants from 21 national and regional frozen semen producers participated in this study. Sperm motility and concentration were assessed conventionally (using a Neubauer chamber) and AndroScope was used with five replicates. The results of the comparison of sperm motility scores revealed significant differences (p<0.05) between conventional examination and using AndroScope. The results of the comparison of the sperm concentration calculations revealed no significant difference (p>0.05) between the calculations performed using the Neubauer chamber and AndroScope. Assessment with the AndroScope is considered reliable and can replace conventional assessment in assessing motility and sperm concentration, thereby improving and standardizing the assessment performed by frozen semen producers.
Co-Authors . Soeparna A.A. Ketut Agung Cahyawan W Abdullah Baharum Abdullah Baharun Agasi, Satri Yusasra Amani, Aqila Zata Aminudin Aminudin Anak Agung Gede Sugianthara Andriani Andriani Angelina N. Tethool Anna Nabilla Anny Amaliya Asep Gunawan Asep Kurnia Asep Kurnia Azizah - Baharum, Abdullah Bambang Purwantara Bq Hayyul Hidayati Cece Sumantri Damiana Rita Ekastuti Dedi Rahmat Setiadi DEWI APRI ASTUTI Diana Andrianita Kusumaningrum Dondin Sajuthi Eko Prasetyo Nugroho Elmanaviean, Muhammad Emilia Kamung Hambu, Emilia Kamung Enniek Herwiyanti Faisal Amri Satrio Frederika Katerina Foeh, Nancy Diana Gatot Prasojo GONO SEMIADI Gunawan Sitanggang Gusdinar, Rizal Gusman, Khalis Talitha Hasbi Hasbi Hastuti, Yohana Tri Henny Nuraini herdis herdis Herry Sonjaya Hikmayani Iskandar Imam Purwadi, Imam Iman Supriatna Indriastuti, Rhesti Jakaria Jakaria Jansen Manansang Jansen Manansang Jean-Baptise Menassol Junaedi Junaedi Junaedi Junaedi Ketut Adnyane Mudite Khaeruddin Khaeruddin Khaeruddin Khaeruddin Komariah Komariah Kurnia Bagus Ariyanto Kusdiantoro Mohamad Laksono, Mohamad Nanang Tejo Langgeng Priyanto Lilis Khotijah Magfira Magfira Magfira Magfira Magfira Maghfira Maghfira Manansang, Willem Memili, Erdogan Mohamad Yamin Mohamad Yamin Mohamad Yamin Mokhamad Fahrudin MUHAMMAD AGIL Muhammad Agus Tahar Muhammad Gunawan Mulia, Bongot Huaso Muttaqinullah Rabusin Nancy D. F. K. Foeh Ni Wayan Karniani Karja Ni Wayan Kurniani Karja Niken Ulupi Nila Pratiwi Nugraha, Arifin Budiman Nur Hidayati Nurcholis Nurcholis Nurcholis Nurcholis Nurcholis Nurcholis Nuri Andarwulan Oriza Savitri Ariantie Palupi, Nurheni Sri Pardede , Berlin Pandapotan Pardede, Berlin Pandapotan Prayetno, Saptohadi Puwantara, Bambang Rahmat Hidayat Rahmat Hidayat Reni Ratni Dapawole Rhesti Indriastuti Rhomadon, Gilang Ria Ariyanti Ristika Handarini Riwu, Ronny Markus Jami Rudi Afnan Santoso Santoso Setyaningsih Rambu Liwa Siti Zubaidah Soeparna Soeparna Sri Darwati Sri Darwati Srihadi Agungpriyono Syahruddin Said Teguh Ari Prabowo Tejolaksono, Muhammad Nanang Tike Sartika Tike Sartika Triansyah, Irvan Tulus Maulana Tuti Suryati Tuty Laswardi Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf TUTY LASWARDI YUSUF Tuty Laswardi Yusuf TUTY LASWARDI YUSUF Tuty Laswardy Yusuf Uus Saefullah Wahono Esthi Prasetyaningtyas Widihasputri, Risti Wilmientje Marlene Mesang Nalley Witri, Brilla Widya Yohana Tri Hastuti Yulida Nofa Yusman Setiawan