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All Journal Jurnal Ilmu Pertanian Indonesia BALI MEDICAL JOURNAL (BMJ) Jurnal Ilmu dan Kesehatan Hewan METAMORFOSA Journal of Biological Sciences Buletin Veteriner Udayana Buletin Udayana Mengabdi INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Jurnal Veteriner Indonesia Medicus Veterinus Jurnal Biologi Udayana Bumi Lestari Medical Journal of Indonesia Jurnal Ilmu Ternak Veteriner Journal of Global Pharma Technology Jurnal Kedokteran Hewan Neurona Jurnal Medika Veterinaria
I Gusti Ngurah Kade Mahardika
Fakultas Kedokteran Hewan Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health Veterinary

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Pengetahuan Masyarakat Tentang Rabies Dalam Upaya Bali Bebas Rabies I Nyoman Suartha; Made Suma Anthara; I Gusti Ngurah Narendra Putra; Ni Made Ritha Krisna Dewi; I Gusti Ngurah Kade Mahardika
Buletin Veteriner Udayana Vol. 4 No.1 Pebruari 2012
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (49.235 KB)

Abstract

This study aims to determine the level of public knowledge about rabies in an effort to Balirabies-free. The data were collected from three villages namely Kukuh in Tabananregency, Jagapati in Badung regency, and Seraya in Karangasem regency. The mentiontotal of responden are 991 people. Data were collected using questionnaire. Results showedthat respondents who do not know about the news that rabies has been in outbreak in Balias much as 33.3%. The sources of information about the disease of rabies came from themedia (TV, newspapers, radio) as much as 44%, and 31% from health extension program.First aid to do if bitten by a dog was wash wound with soap (80%), but there were alsoresponden who do not care for themself (4%). Most responden would go to health centerfor treatment (85%) if they were bitten by rabies dogs. But , there were also few respondenwho take no care and do nothing (7%). Public knowledge about the clinical symptoms of rabies was still low (53%), and as much as 39% of respondents did not know the signs ofrabies. Knowing of report place if there was an event of rabies or finding a dog with rabieswas to the head of the village (41%), to the Department of Animal Husbandry (39%) andnot reporting 4%. The conclusion of this study is the public knowledge about the disease ofrabies is moderate.
Gambaran Darah pada Babi yang Diinfeksi Streptococcus suis Secara Intranasal dan Intravena Putri Wiliantari; I Nengah Kerta Besung; Gusti Ngurah Kade Mahardika
Buletin Veteriner Udayana Vol. 14 No. 3 June 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (129.695 KB) | DOI: 10.24843/bulvet.2022.v14.i03.p12

Abstract

Research has been carried out to elucidate clinical, as well as differences in blood profiles in pigs infected with Streptococcus suis isolated from Bali by intravenous and intranasal infection routes. S.suis isolate of PPB5 were inoculated intranasally and intravenously to three pigs respectively. The pigs used in this study were 8 weeks old. In intranasal infection before inoculation, 1% acetic acid was first applied to the nasal cavity, then S. suis inoculum was sprayed (2 x 106). In intravenous infection, inoculum fluid is injected into the auricular vein. Clinical changes in pigs were observed until day 15. Blood samples were taken before being inoculated with bacteria, 3rd day after inoculation, and before being euthanized. On the 3rd day after inoculation, the number of eosinophils was significantly different in the two treatments, while the other blood profiles were not significantly different. All blood indicators in the two treatments were not significantly different before the animals were euthanized on day 15. The increase number of erythrocytes and monocytosis was found in intranasal infections at that day. Hypohaemoglobinemia and decreased PCV are seen in both intranasal and intravenous infections. Further research is needed with a greater number of replications. The S. suis vaccine needs to be developed immediately to reduce the threat to public health and economic losses for farmers.
Analisis Marka Gen Patogenik iutA Escherichia Coli Penyebab Colibasilosis pada Ayam Buras Kadek Satria Adi Marhendra; I Gusti Ngurah Kade Mahardika; I Nengah Kerta Besung; I Gusti Ketut Suarjana
Buletin Veteriner Udayana Vol. 13 No. 1 Pebruari 2021
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (593.228 KB) | DOI: 10.24843/bulvet.2021.v13.i01.p10

Abstract

Colibacillosis is an infectious disease in chickens caused by Avian Pathogenic Escherichia coli (APEC). The ability of APEC to cause disease depends on many pathogenic factors, one of which is the iutA pathogenic gene. This study was purposed to determine the DNA sequence of the APEC iutA gene in Bali and its kinship with the iutA gene from other countries. Two APEC isolates from free-range chicken in Tabanan dan Badung has been used. The isolates have been purified and were available at the Bacteriology Laboratory of Veterinary Medicine Faculty, Udayana University. Chelex 10% was used for DNA isolation. DNA amplification using published DNA primer has been conducted with the polymerase chain reaction (PCR) method. The PCR product was sequenced at Malaysia, First Base Laboratories using Sanger’s Dideoxy Nucleotide Termination method. The iutA gene of both isolates can be analyzed and have 250 bp in length. Both were 100% homologous. A phylogenic test using 58 DNA sequences of iutA gene from Escherichia coli and other bacteria in the world was conducted in MEGA 5.2. All data have 43 polymorphic sites of nucleotide acid and 13 polymorphic sites of amino acid. The iutA gene from Bali is in one group, with the iutA gene from Brazil (KP657535) in 2011, Germany (LT599825) in 2016, and China (CP033635) in 2016. This gene can be used as a pathogenic marker of APEC in Indonesia.
Deteksi dan Sekuensing Gen iroN, iutA, dan hlyF pada Avian Pathogenic Eschericia coli Nyoman Anandiya Ramaditya; I Nengah Kerta Besung; I Gusti Ngurah Kade Mahardika
Buletin Veteriner Udayana Vol. 11 No. 2 Agustus 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (263.69 KB) | DOI: 10.24843/bulvet.2019.v11.i02.p16

Abstract

Research has been carried out to detect pathogenic gene markers of Avian Pathogenic Eschericia coli (APEC) iroN, iutA, and hlyF in Escherichia coli bacteria isolated from organs of sick chickens in Bali, and to determine phylogenetic relationships between those marker genes in Bali and in the other countries in the world. Six isolates of E. coli bacteria with codes E2, E3, E7, E8, E9, and E10 were used in this study. The isolates were isolated from domestic chicken in 2018. All genes were detected using the Polymerase Chain Reaction (PCR) method. The genes of iroN, iutA, and hlyF could be detected from all isolates. Well readable sequence of iroN, hlyF, and iutA was 659 bp, 518 bp, and 250 bp, respectively. All three genes were homogenous. Phylogical analysis shows that all pathogenic markers share same cluster with the pathogenic E. coli from all countries in the world.
Analisis Filogeni Virus Newcastle Disease Isolat Bali Tahun 2013 Sampai 2014 Berdasarkan Sekuen Daerah Pemotongan Protein Fusion Fedri Rell; Anak Agung Ayu Mirah Adi; I Gusti Ngurah Kade Mahardika
Buletin Veteriner Udayana Vol. 13 No. 1 Pebruari 2021
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (437.757 KB) | DOI: 10.24843/bulvet.2021.v13.i01.p11

Abstract

Newcastle disease is a contagious disease caused by the Avian paramyxovirus serotype 1 virus, which infected the poultry. The aims of this study were conducted to phylogenetic analysis of the fusion protein cleavage site of Newcastle disease virus found in a chicken farm in the province of Bali throughout the year 2013 to 2014. There are four isolates from sick chicken’s cases/death suspected of being infected by the Newcastle disease virus. The sequence of the protein fusion was aligned and edited using MEGA5. The amino acid sequence of the F cleavage site of all isolates was 112R-R-Q-K-R-F117 and analysis of phylogeny tree, which justified that all four are virulent of Newcastle disease virus. It is concluded that four Bali isolates in 2013 to 2014 under this study are Newcastle disease virus of genotype VII.
Seroprevalensi Penyakit Avian Influenza Pada Itik Di Kabupaten Klungkung Estry Gusnita Damanik; Gusti Ayu Yuniati Kencana; I Gusti Ngurah Kade Mahardika
Buletin Veteriner Udayana Vol. 5 No. 2 Agustus 2013
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

Itik memiliki peran penting dalam penyebaran virus Avian Influenza subtipe  H5N1 karena merupakan reservoir alami virus dan infeksinya bersifat subklinis. Pendistribusian itik terjadi dari pasar unggas ke peternakan itik atau sebaliknya. Penelitian ini bertujuan untuk mengetahui perbedaan tingkat seroprevalensi virus Avian Influenza di Pasar Unggas Galiran dan peternakan itik di kabupaten  Klungkung pada saat yang bersamaan.  Sampel penelitian adalah serum dari itik yang tidak divaksin yang diambil dari pasar dan peternakan di kabupaten Klungkung.  Pengambilan  sampel dilakukan setiap bulan mulai bulan Maret sampai dengan bulan Agustus 2012.  Sampel serum selanjutnya  diuji dengan uji Hambatan Hemaglutinasi. Hasil penelitian menunjukkan seroprevalensi AI di Pasar Unggas Galiran dan peternakan itik di Kabupaten Klungkung adalah sebesar 81.4%,  perbedaan yang signifikan terjadi pada Juni dan Agustus tetapi tidak signifikan pada bulan Maret, April, Mei, dan Juli.  Seroprevalensi virus AI di Pasar Unggas Galiran adalah sebesar 76.2% dan di peternakan sebesar 86.7% dan secara statistik berbeda sangat nyata. Monitoring terhadap virus Avian Influenza berkelanjutan perlu dilakukan baik di peternakan maupun  di pasar unggas di Klungkung.
PEMBINAAN KELOMPOK TANI DENGAN PENYULUHAN PENINGKATAN KESEHATAN TERNAK SAPI DAN PEMANFAATAN LIMBAH TERNAK MENJADI KOMPOS G.A.Y. Kencana; I.G.N.K. Mahardika Mahardika; I.N.M. Astawa; I.B.K. Suardana; I.N. Suartha; I.A.P. Apsari; A.A.S. Kendran; S.K. Widyastuti; G.A.M.K. Dewi; I.P. Sudiarta
Buletin Udayana Mengabdi Vol 20 No 1 (2021): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1385.678 KB) | DOI: 10.24843/BUM.2021.v20.i01.p13

Abstract

Pengabdian Masyarakat berjudul: "Pembinaan Kelompok Tani Niti Sari Desa Baturiti, Kecamatan Baturiti” merupakan salah satu Hibah Program Udayana Untuk Masyarakat (PUMA). Tujuan pengabdian ini untuk memberikan pembinaan kepada Kelompok Tani Niti Sari tentang cara meningkatkan kesehatan sapi dan memanfaatkan limbah pertanian menjadi kompos organik plus. Metode yang digunakan untuk mencapai tujuan tersebut diawali dengan memberikan penyuluhan kepada anggota Kelompok Tani Niti sari, selanjutnya didukung dengan praktek langsung di lapangan. Penyuluhan yang diberikan meliputi: Penyuluhan kesehatan sapi, penyuluhan kesehatan reproduksi sapi, penyuluhan teknologi tepat guna pertanian dengan memanfaatkan limbah pertanian menggunakan jamur Trichoderma untuk dibuat kompos plus. Kompos plus Trichoderma yang dihasilkan dalam pembinaan ini diharapkan dapat mengatasi permasalahan penyakit tanaman kubis milik petani Niti Sari. Penyuluhan sudah dilaksanakan tanggal 05 Juli 2019 diawali pertemuan dengan Kepala Desa Baturiti, dengan Kelompok Tani Niti Sari dan dilanjutkan dengan pembinaan Kelompok Tani. Kegiatan penyuluhan dibuka oleh Camat Baturiti, Ketua LPPM Unud, Tripika Kecamatan Baturiti, Kepala Desa beserta aparat Desa, Kelompok Tani Niti Sari dan Kelompok Tani se Desa Baturiti dan Banjar Tamantanda. Dengan demikian diharapkan Kelompok Tani Niti Sari dan Kelompok Tani disekitarnya mampu menyerap alih teknologi yang diajarkan oleh Tim Pengabdi dari Universitas Udayana.
Penerapan Teknologi Reproduksi untuk Mempertahankan Ketersediaan Ayam Upakara di Bali I N. Suartha; I W Bebas; I. G.N.K. Mahardika
Buletin Udayana Mengabdi Vol 15 No 1 (2016): Jurnal Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

Chicken is important part in Balinese Hindu ceremony. Chicken were used as ceremony are chicken with black feather (siap selem), red feather (siap biing), white feather (siap putih) that a white feather chicken with white colour feet, yellow feather (siap putih siungan), that a white feather chicken with yellow colour feet, and chicken with mixed colour of those four colour (siap brumbun). Limitations of technology owned by community makes the chicken’s breeding doesn’t provide economic added value. The purpose of this service is to provide innovation of artificial insemination technology of chicken production for ceremony. The Innovation is given through counseling and workshops . The results showed that people knowledge about disease and breeding management was increased. The farmer have been able to do artificial insemination and vaccination in chicken. The participant were very enthusiastic in following the activities and understand the disease and how to prevent diseases in chickens. These skills are need to trained constantly to find the comfortable thechnique. The conclusions is farmers have been able to perform artificial insemination in chicken.
Identifikasi Spesies Ikan Kerapu di Pasar Ikan Karangasem dan Kedonganan Bali Menggunakan DNA Mitokondria Gen 16s rRNA IDENTIFICATION OF GROUPER SPECIES USING 16S rRNA MITOCHONDRIAL DNA IN KARANGASEM AND KEDONGANAN FISH MARKET, BALI Ni Luh Made Ika Yulita Sari Hadiprata; I Made Bagus Arya Permana Ardiana Putra; I Gusti Ngurah Kade Mahardika; I Nengah Wandia; Tjokorda Sari Nindhia
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Groupers are bio-indicators of the health of coral ecosystems and high economic value. Various speciesof grouper fish sold in markets in Indonesia is not known. The purpose of this study was to identify thespecies of grouper fish market located in Karangasem and Kedonganan. The method used in this studywas polymerase chain reaction(PCR) techniqueand mitochondrial DNA 16S rRNA gene as a molecularmarker. Grouper samples were collected from the fish market Karangasem total of 11 samples and 42samples collected from Kedonganan. Sequences from each sample was obtained from the analysis inBerkeley Sequencing Facility, California. Sequencing samples were then analyzed by BLAST and matchedwith the sequences found in GenBank. The analysis showed that six species can be identified from the fishmarket Karangasem namely Cephalopholis leopardus, C.cyanostigma, C. miniata, C. sonnerati, Variolaalbimarginata, and Epinephelus fasciatus. Seven species can be identified in the fish market Kedonganannamely E. fuscoguttatus, E. coeruleopunctatus, E. merra, E. polyphekadion, C. cyanostigma, C. miniata andV.albimarginata.
Pengaruh Pemberian Laktoferin Sapi Terhadap Berat Badan Dan Leukosit Anak Babi Ghina Monita Pramudhita; I Gusti Ngurah Kade Mahardika; I Gusti Ayu Agung Suartini
Jurnal Veteriner Vol 22 No 3 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (107.186 KB) | DOI: 10.19087/jveteriner.2021.22.3.449

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian pakan yang ditambahkan laktoferin sapi terhadap berat badan dan leukosit anak babi Landrace. Penelitian dilakukan pada peternakan komersial di Desa Yeh Gangga, Kecamatan Sudimara, Kabupaten Tabanan. Sebanyak 12 anak babi Landrace dari tiga induk yang berbeda digunakan sebagai sampel, dengan pembagian dua kelompok, yaitu kelompok kontrol dan perlakuan. Kelompok kontrol tidak diberikan perlakuan. Kelompok perlakuan diberikan laktoferin sapi selama 7 hari dengan pemberian satu kali dalam satu hari. Penimbangan berat badan anak babi Landrace dilakukan setiap lima hari, kemudian data dianalisis dengan Student’s T-test. Hasil analisis data periode penimbagan berat badan anak babi umur 10 – 35 hari berpengaruh nyata (P<0,05). Pengabilan darah dilakukan pada post perlakuan kemudian diuji dengan uji hematologi. Data yang diperoleh dianalisis menggunakan Student’s T-test. Hasil penelitian menunjukkan bahwa rerata diferensial leukosit tidak berbeda nyata (P>0,05) antara kelompok kontrol dan kelompok perlakuan. Masing-masing komponen leukosit pada kelompok kontrol maupun kelompok perlakuan berada pada rentang nilai normal. Rerata total leukosit anak babi pasca pemberian laktoferin tidak berbeda nyata (P>0,05) antara kelompok kontrol dan kelompok perlakuan.
Co-Authors Agik Suprayogi Agus Eka Darwinata Amelia, Ni Kadek Shita Anak Agung Ayu Mirah Adi Anak Agung Gde Oka Dharmayudha Anak Agung Gde Putra ANAK AGUNG NGURAH GEDE DWINA WISESA ANAK AGUNG NGURAH OKA PUJAWAN Anak Agung Sagung Istri Pradnyantari Anak Agung Sagung Kendran Andi Bahtiar Batti Anita Dwi Handayani Bambang Sumiarto Bhaskara, Audrey Febiannya Putri Brigita Galilea Adu Charles Rangga Tabbu Daud Steven Triyomi Hariyanto Dewi, Putu Bulan Sasmita DWI SURYANTO Estry Gusnita Damanik F. S. Wignall Fakhrurrasi Fakhri Fedri Rell G.A.M.K. Dewi Ghina Monita Pramudhita Gusti Ayu Dianti Violentina Gusti Ayu Mayani Kristina Dewi Gusti Ayu Yuniati Kencana Gusti Ngurah Narendra Putra Helen Scott-Orr Herawati Sudoyo Heru Susetya I Dewa Made Sukrama I GBA Purwanda I Gede Eka Chandrawan I Gusti Agung Ayu Suartini I Gusti Kamasan Nyoman Arijana I Gusti Ketut Suarjana I Gusti Ketut Suarjana I Gusti Made Krisna Erawan I Gusti Ngurah Badiwangsa Temaja I GUSTI NGURAH DIBYA PRASETYA I Gusti Ngurah Narendra I Gusti Ngurah Narendra Putra I Gusti Ngurah Narendra Putra I Gusti Ngurah Narendra Putra I Gusti Ngurah Narendra Putra1, I K. Suata I KADEK SAKA WIRYANA I Ketut Berata I Made Bagus Arya Permana Ardiana Putra I Made Kardena I Made Sara Wijana I Made Sukada I MADE SUMA ANTARA I Made Suma Anthara I Nengah Kerta Besung I Nengah Kerta Besung i Nengah Wandia I NYOMAN ADI SURATMA I Nyoman Dibia I NYOMAN MANTIK ASTAWA I Nyoman Suartha I Putu Sudiarta I Wayan Bebas I Wayan Gorda I Wayan Masa Tenaya I wayan Teguh Wibawan I Wayan Wirata I-W.T Wibawan I. B. P. Dwija I. K. Suastika I.A.P. Apsari I.B. Oka Suyasa I.B.K. Suardana Ida Ayu Pasti Apsari Ida Ayu Sri Candra Dewi Ida Ayu Sri Chandra Dewi Ida Bagus Kade Suardana Ida Bagus Komang Ardana Ida Bagus Oka Winaya Indrawati Sendow Inna Narayani K. Subrata K. Wirasandhi Kadek Karang Agustina Kadek Satria Adi Marhendra Ketut Tuti Parwati Merati Ketut Wella Mellisandy Lies Parede Luh Made Sudimartini Lusiana Lasmari Siahaan M.T Suhartono MADE PHARMAWATI MADE RATNA SARASWATI . Made Suma Anthara Maggy Thenawidjaja Suhartono Martien Herna Susanti Melkias Oagay Melkias Oagay Messy Saputri Boru Sembiring N. K. Susilarini N. Sri Budiyanti Nareswari, Ayu Widya Ni Ketut Dias Nursanty Ni Ketut Suwiti Ni Komang Eka Agustiani Ni Luh Made Ika Yulita Sari Hadiprata Ni Luh Putu Agustini Ni Luh Watiniasih Ni Luh Wayan Yulia Mirayanti Ni Made Adi Tarini Ni Made Krisna Dewi Ni Made Rita Krisna Dewi Ni Made Ritha Krisna Dewi Ni Made Ritha Krisna Dewi Ni Made Ritha Krisna Dewi Ni Made Ritha Krisna Dewi2 Ni Made Susilawathi Ni Nengah Dwi Fatmawati Ni Nyoman Sri Budayanti Ni Putu Dian Pertiwi Ni Putu Sriwidyani Nyoman Anandiya Ramaditya Oktryna Hodesi Sibarani Pieter Mbolo Maranata Pipit Dwi Pramesti Pramitasuri, Tjokorda Istri Putri Wiliantari Putu Mei Purnama Dewi R Susanti R.D Soejoedono Raka-Sudewi A. A. Rd Soejoedono Retno Damajanti Soejoedono S Murtini S.K. Widyastuti Safarina G. Malik Sayu Raka Padma Wulan Sari, Sayu Raka Padma Wulan Sri Kayati Widyastuti sri murtini . Sukma Oktavianthi Susilawathi, Ni Made Tania Ria Gunawan TJOK GEDE OKA PEMAYUN Tjokorda Sari Nindhia TRI KOMALA SARI Wibawan IWT Widya Asmara Yan Ramona Yosaphat L.S Kote