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Kualitas Spermatozoa Post Thawing Semen Beku Sperma Y Hasil Sexing Pada Sapi Limousin Ali Mahfud; Nurul Isnaini; Aulia Puspita Anugra Yekti; Kuswati Kuswati; Trinil Susilawati
TERNAK TROPIKA Journal of Tropical Animal Production Vol 20, No 1 (2019): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtapro.2019.020.01.1

Abstract

Kualitas spermatozoa pada semen beku sexing sangat penting dalam menunjang keberhasilan inseminasi buatan untuk mendapatkan pedat dengan jenis kelamin sesuai harapan. Tujuan penelitian ini bertujuan untuk mengetahui kualitas semen beku semen beku non sexing dan sexing secara makroskopis dan mikroskopis. Materi dalam penelitian menggunakan starw semen beku non sexing dan sexing dengan metode pemisahan Sentrifugasi Gradien Densitas Percoll (SGDP) sapi Limousin yang diproduksi oleh Balai Besar Inseminasi Buatan (BBIB) Singosari, Malang. Metode penelitian ini menggunakan eksperimental dengan percobaan labolatorium (laboratory experiment). Variabel penelitian meliputi keadaan umum meliputi tahun produksi straw, secara makroskopis meliputi pH semen beku dan secara mikroskopis meliputi persentase motilitas, viabilitas, abnormalitas, konsentrasi dan total spermatozoa motil. Data yang diperoleh diolah Microsoft Excel dengan analisis Ragam Analysis of variance (Anova) single factor dianalisis secara deskriptif. Hasil penelitian yaitu semen beku non sexing dan sexing meliputi keadaan umum diproduksi tahun 2012 dan 2018, kualitas secara makroskopis memiliki nilai pH sebesar 6,67 dan 6,60 dan kualitas secara mikroskopis memiliki persentase motilitas sebesar 36,00 % dan 31,40 %, viabilitas sebesar 81,70 % dan 75,89 %, abnormalitas sebesar 6,93 % dan 6,78 %, konsentrasi spermatozoa sebesar 31,67 juta/ mini straw dan 12,125 juta/ mini straw dan total spermatozoa motil sebesar 11,39 juta/mini straw dan 5,10125 juta/mini straw. Kesimpulan penelitian bahwa Kualitas post thawing semen beku non sexing lebih tinggi daripada semen beku sexing menggunakan metode SGDP baik secara makroskopis maupun mikroskopis.
ESTIMATION OF HERITABILITY AND BREEDING VALUE OF PIGLET BIRTH WEIGHT Bayu Dewantoro Putro Soewandi; V. M. Ani Nurgiartiningsih; Kuswati Kuswati
Widyariset Vol 3, No 2 (2017): Widyariset
Publisher : Pusbindiklat - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (633.627 KB) | DOI: 10.14203/widyariset.3.2.2017.143-150

Abstract

Piglet birth weight has an economic value and commonly correlated with weaning weigh. Therefore, birth weight traits of piglet needs to be selected for genetic improvement. The purpose of a study was to estimate the genetic improvement of piglet birth weight based on heritability and breeding value. Around 154 heads of offsprings generated from 6 sires and 14 sows were used in this study. The data of piglet birth weight was analyzed for heritability (nested classification), breeding value, selection response, and genetic improvement estimation. The result showed that the heritability (h2) of offsprings was 0.24±0,15. Based on breeding value, sires which had high piglet birth weight can be used for male ranking. Selection of response value was 0.06 kg. According to the selection of response value, improvement of birth weight in piglet based on the sire used can be estimated.
Pengaruh Penambahan Fruktosa dalam Pengencer Air Kelapa Hijau terhadap Motilitas Spermatozoa Sapi PO (Peranakan Ongole) D. Muhammad; N. Isnaini; Kuswati Kuswati; A. P. A. Yekti; Aryogi Aryogi; M. Luthfi; L. A. Sunarto; T. Susilawati
Jurnal Sain Peternakan Indonesia Vol 13, No 4 (2018)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.13.4.318-324

Abstract

Air kelapa merupakan bahan lokal yang murah dan dapat menjadi bahan baku alternatif dalam proses pembuatan pengencer semen karena mengandung beberapa zat yang dibutuhkan oleh spermatozoa seperti sumber energi berupa fruktosa. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan fruktosa dalam pengencer air kelapa hijau terhadap motilitas spermatozoa sapi PO (Peranakan Ongole). Penelitian ini menggunaan semen dari bangsa sapi Peranakan Ongole yang ditampung menggunakan metode vagina buatan. Metodologi yang digunakan adalah eksperimental laboratorium menggunakan rancangan acak kelompok (RAK). Penelitihan ini menggunakan 3 perlakukan, P1 = Pengencer air kelapa +  kuning telur20%, P2 = Pengencer air kelapa + kuning telur 20% + putih telur 0,4%+ fruktosa 1 mg/ml. P3 = Pengencer air kelapa +kuning telur20% + putih telur 0,4% + fruktosa 2 mg/ml dengan 10 ulangan. Hasil memperlihatkan bahwa tidak terdapat perbedaan yang signifikan pada motilitas spermatozoa dan total spermatozoa motil antara formulasi pengencer air kelapa (P1, P2, P3) selama peyimpanan suhu 2-5 oC. Pengencer air kelapa baik dalam menjaga motilitas spermatozoa dan total spermatozoa motil hingga hari ke lima.Kata kunci: air kelapa, total motilitas motil, semen cair
Tingkat keberhasilan Inseminasi Buatan (IB) menggunakan semen beku hasil sexing pada Sapi Persilangan Ongole Ali mahfud; Trinil Susilawati; Aulia Puspita Anugra Yekti; Kuswati Kuswati
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 29, No 2 (2019): Agustus
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2019.029.02.10

Abstract

The purpose of artificial insemination using  sexing semen was to obtain the calf according to the expected sex. This study aimed to determine the success of artificial insemination in non-sexing and sexing of frozen semen. The materials used in this research were 77 non-sexing artificial insemination acceptor cows and 77 sexing artificial insemination acceptor cows with double dose treatment. The method used in this study was experimental field experiment with the research treatment: P0 = frozen non-sexing semen straw and P1 = frozen semen of spermatozoa Y by sexing method using Percoll Density Gradient Centrifugation (SGDP) produced by Balai Besar Inseminasi Buatan (BBIB) Singosari. The variables of this study were Non Return Rate (NRR), Service Per Conception (S/C) and Conception Rate (CR). The data obtained were analyzed descriptively. The results of the study in each non-sexing and sexing artificial insemination treatment were: the percentage of NRR 1 were 90.91% and 77.92%, followed by NRR 2 with 88.31% and 76.62%, the pregnancy percentage were 84.42% and 75.33%, and the CR values were 79.22% and 57.14%. The conclusion of this study was artificial insemination using frozen non-sexing semen had a higher success rate compared to artificial insemination using frozen sexing semen.
Performance of Male Limousine Crossbreed Cattle by Giving RD Flour at Different Levels Anggun Permata Sari; Tri Eko Susilorini; Mashudi Mashudi; Kuswati Kuswati
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 32, No 1 (2022): April 2022
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2022.032.01.13

Abstract

The study aimed to evaluate the production performance of male Limousine Crossbreed cattle with different feed levels with the addition of RD flour. It used nine male Limousine crossbreed cattle, aged <1-3 years, with an average initial weight of 335±29,09kg. The method used is an experimental method using CRD with 3x3: T0= Ad libitum corn straw + concentrate without RD powder, T1= Ad libitum corn straw + concentrate containing 7,5% RD, T2= Ad libitum corn straw + concentrate containing 15% RD flour. The variables measured included Daily gain, Average daily gain, feed conversion ratio, and Income over feed cost. Data were analyzed by ANCOVA. Cattle were fed for eight weeks from (March 26 to June 8, 2021), in the Livestock Group of Kucur Village, Dau District, Malang Regency. The result showed that using feed with the addition of RD at different levels of male Limousine crossbreed cattle had a significant effect on FCR (P>0,05) and on Daily gain, Average daily gain, and IOFC (P<0,01) had a different significant effect. This study concluded that the treatment of T1 feed (7.5% "RD" flour) showed the best results for Daily gain (70,67±5,43 kg), Average daily gain (1.26±0.09 kg), FCR (5.85±0.19),, IOFC Rp. 36586.50.
UJI AKURASI PIRANTI SENSOR TRANSDUSER UNTUK MENENTUKAN WAKTU TERJADINYA BERAHI PADA SAPI Suyadi Suyadi; Kuswati Kuswati; B. Susilo; J. A. E. Noor
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 20, No 1 (2010)
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The success of estrus detection ensure the correct time of insemination so the high conception rate can be resulted. This research was objected to design an electronic equipment as sensor transducer for measuring the change of circular pressure of vaginal wall of the cows. A sensor used was Load cell type M322. The sensor was connected to microcontroller and a display. The results showed that the applied sensor on a stainless steel transducer steak with a diameter of 2.5 cm and length of 40 cm measured a sensitive change of pressure attach it. This sensitivity was tested to measure the change of pressure of different dept of water i.e. 0, 10, 20, 30, 40 and 50 cm. Two equations was resulted from this measurement, while a linear regression was Y= -36.412+197.53X a quadratic equation was Y= 253.3+84.09X+5.09X2. The sensor was then applied in estrus beef cow, not estrus beef cow and estrus dairy cow for 10 days. The same pattern was observed in estrus of both beef and dairy cows: a high values were detected in the non estrus phase while during estrus phase the values were in basal. In contrast, anestrus cow showed high value during 10 days observation, and 5 min application of each. In conclusion, this designed estrus detector was possible to be developed toward application in the farmers after some modifications were created. (JIIPB 2010 Vol 20 No 1: 11-21). Key words : sensor transducer, uterine wall pressure, estrus-non estrus cows
Evaluation of breeding value based on body weight and body measurement at one day of age in Madura cattle Zulfaini Shamad; Kuswati Kuswati; Ahmad Furqon; Priyo Sugeng Winarto; Agus Susilo; Veronica Margareta Ani Nurgiartiningsih
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 31, No 3 (2021): December 2021
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2021.031.03.08

Abstract

The objective of this research was to evaluate the breeding value based on body weight and body measurement at one day of age in Madura cattle on the Technical Implementation Unit of Animal Breeding and Health East Java. Recording data used were withered height (WH), body length (BL), chest girth (CG), and birth weight (BW) that birth from 2014 until 2020.  Analyzed variables consist of heritability, breeding value, and spearman correlation. The analysis result showed heritability for the performance of 1 day of age in Madura cattle categorized as a medium until high (0.33 – 0.64). Breeding value from 10 bull based on BW was 60% categorized positive. Meanwhile, breeding value based on WH, BL, CG was 40% categorized positive. The Spearman correlation of BW with WH and CG had a high correlation (0.73 and 0.78), while BW and BL had a low correlation (0.22). This research concluded that the highest breeding value of body weight and body measurement of Madura cattle at 1 day was sire Montahai/1661100. The genetic potency of Madura cattle should be improved by applying for a consistent breeding program with a sound recording and selection program.
Pengaruh kastrasi terhadap performan produksi Sapi Persilangan Wagyu berdasarkan umur yang berbeda Kuswati Kuswati; Ravenska Ravenska; Nareswara Hapsari; Aulia Puspita Anugra Yekti; Trinil Susilawati
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 26, No 3 (2016)
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2016.026.03.08

Abstract

The purpose of this research was to know the effect of castration to Wagyu crossbreed cattle production performance based on age difference and know the appropriate age to do castration. The materials used in this research were 37 heads of 3-month old and 28 heads 4 months calf castration. The method was case studies with purposive sampling. Data were analyzed using unpaired t test. The results showed that the calf castration age of 3 months and 4 months showed significantly different (P <0.05) in daily body weight gain and feed conversion. The average daily weight gain of 3 months old calf castration was 0.78 kg / head / day and 4 months old calf castration was 0.85 kg / head / day and feed conversion of 3 months old calf castration was 5.14 and 4 months old calf castration was 4.48, while the feed intake of the research results were not significantly different. The average feed consumption of 3 months old calf castration was 3.82 kg / head / day and 4 months old calf castration was 3.66 kg / head / day. Calves were castrated on 4 months old showed higher daily weight gain than the calf castration on 3 months old. This could be concluded that 4 months old was more appropriate to do castration. Keywords: Average daily gain, calf stock, feed convertion, castration
Pengaruh lama istirahat terhadap karakteristik karkas dan kualitas fisik daging sapi Brahman Cross Steer Mukhlas Agung Hidayat; Kuswati Kuswati; Trinil Susilawati
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 25, No 2 (2015)
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2015.025.02.09

Abstract

The purposes of this study were determine the carcass characteristics and physical meat quality of Brahman Cross Steer on different rest periods. The experiment was conducted on June 2015 at Slaughter house Cianjur Arta Makmur Ltd. in the Cianjur District, West Java. The total number of cattle used in the study was 30 heads of Brahman Cross Steer treated for 18 hours lairage duration and 30 heads of Brahman Cross Steer treated for 3 hours lairage duration from Pasir Tengah feedloter Ltd (Holding Widodo Makmur Perkasa Ltd). The method used a case study. Data were analyzed using unpaired t test. The results showed that the carcass characteristics including slaughtered weight, hot carcass weight, chilled carcass weight and carcass weight loss percentage were not significantly different, while the dressing percentage for 18 hours lairage duration (54.16±1.62%) and 3 hours lairage duration (55.60±2.00%) was significantly different (P<0.05). Physical meat quality including cooking loss and drip loss was not significantly different, while pH on 18 hours lairage duration and 3 hours lairage duration was significantly different (P<0.05). The pH value of meat derived from cattle which treated for 18 hours lairage duration (5.57±0.09) was higher than that of 3 hours lairage duration (5.42±0.11) although it remained normal for pH (5.4-5.7). It was concluded that 18 and 3 hours lairage duration did not affect slaughtered weight, hot carcass weight, chilled carcass weight, and carcass weight loss, but affected the dressing percentage. In addition, the lairage duration did not affect the cooking loss and drip loss but affected the pH value of the meat. Keywords: drip loss, cooking loss, fattening, pH
Keberhasilan inseminasi buatan menggunakan semen sexing beku pada Sapi Persilangan Ongole Dwi Susan Setiyani; Aulia Puspita Anugra Yekti; Kuswati Kuswati; Trinil Susilawati
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 28, No 3 (2018): Desember
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2018.028.03.09

Abstract

The Artificial Insemination (AI)  results in Beef cattle are expected to be male calf because they can increaseweight gain rapidly, so in this study AI was carried out by using sexing semen with Percoll density gradient centrifugation method containing Y sperm.This study was conducted to evaluate the success rate of AI using frozen sexing semen on Ongole Cross Breed. The study method used wasexperimental, comparing AI frozen sexing semen and frozen semen in 54 Ongole Cross Breed. The study variableswere Non Return Rate1 (NRR1), Non Return Rate2 (NRR2) and Conception Rate (CR).The study showed that the success rate of based on the value of NRR1 and NRR2AI using frozen sexing semen on Ongole Cross Breed is lower than un sexed frozen semen with the NRR1 value is 73,91%; 93,54% while the value of NRR2 was 69,56%; 83,87%. Based on CR value of frozen sexing semen higher than un sexed frozen semen showed CR valuewas 43,47%; 35,48%.
Co-Authors A. P. A. Yekti Abdul Muhaimin Abdul Rohman ACHMAD FARAJALLAH Aditya Kurniawan Agus Susilo Agus Susilo Ahmad Furqon Ahmad Furqon Ajeng G. Nanda, Ajeng G. Ali mahfud Ali Mahfud Alifian Ibnu Ansori Andi Murlina Tasse Andra Widiastutik Andriawan Soni Hantoro Anggun Permata Sari Ardhina Firdausi Aris Sutomo Aryogi Aryogi Aryogi Aryogi Asri Nurul Huda Asri Nurul Huda Aulia Puspita A Yekti Aulia Puspita Anugra Yekti Awang Tri Satria B. Susilo Bayu Dewantoro Putro Soewandi Bella, Frimita D. Muhammad Dandy Prasetyo Dewi , Meinasari kurnia Dian Kumalasari, Elis Dicky Pamungkas Doni Herviyanto Dwi N. Ahsani, Dwi N. Dwi Retna Prihati, Dwi Retna Dwi Susan Setiyani Dwijayanti, Indah Dyah Perwitasari Eko Nugroho Elvira, Ajeng Erin Ayu Octaviani Erlangga Arfiyan Nur Erlia Narulita Ety Sari Handayani Fatimah, Jesy Febrianti, Riska Ayu Ferdy Saputra Fiarani, Hajar Syifa Gani, Tifany Sonia Gatot Ciptadi Hamidah Ikhwan, Salma Nur Hary Nugroho Henik Istikhomah, Henik Hermansyah, Alfandy Herviyanto, Doni Herviyanto, Dony Husnassabil, Ahmad Hadin Ikhsanuddin Ikhsanuddin Ikhsanuddin, Ikhsanuddin Imam Mudakir Indrayani, Tsaniya Ahda Indriyani Indriyani J. A. E. Noor Joko Waluyo Kaiin, Ekayanti Mulyawati Khairul Rasyad Khairunnisa, Icha Kurniaesa, Titah Ulya Kusmartono Kusmartono L. A. Sunarto Latifah Hanum Lina Winarti M. Luthfi M. Nasich Marini . Marjuki Marjuki Mashudi Mashudi Mashudi Mashudi Maulid, Shintia Sukmawati Cahya Moch Nasich Moch Sasmito Djati Moch. Syamsul Arifin Zein, Moch. Syamsul Arifin Mohammad Wildan Habibi, Mohammad Wildan Muhamad Iqbal Zajulie Muhammad Irvan Ali Muhammad Zainul Hanif Mukhlas Agung Hidayat N. Isnaini Nareswara Hapsari Ndaru Pranowo Widiasmoro Nisa’us Sholikah noviyani, ernita prima Nurgiartiningsih, V. M. A. Nurjazuli Nurjazuli Nurmasitoh, Titis Nurrasyidah, Rahmi Nurul Huda, Asri Nurul Insani Shullia Nurul Isnaini Nurul Layla Oktriyani, Erni Onni Meirezaldi Paskah Partogi Agung Pratiwi, Herlina Priyo Sugeng Winarto Puguh Surjowardojo Pujiastuti Pujiastuti, Pujiastuti Purwanto, Didiek Putri Utami Putri Utami Putri, Aprillia Ika Putri, Rizqi Wulandari R Sa’diyah, Kusnul Rahmanti, Fishella Aprista Raihanny, Yasmin Ravenska Ravenska Rifai Mustofa Rini Dwi Wahyuni Rini Dwi Wahyuni Risky Amalia Rosary Rizki Prafitri Rizki Prafitri Rofidaa, Asta Rosalinda, Julitha D Rosalinda, Julitha Dwi Rusdiana, Rina Sa'diyah, Kusnul Rodhiyatul Safitri, Putri Fina A’rafiani Safitri, Sinda Mercy Saputra, Muhammad Rizky Sari, Agustina Siti Hodijah Sri Wahjuningsih Sucik Maylinda Sucik Maylinda Sugesti, Madiyan Surjowadojo, Puguh Sutomo, Aris Suyadi Suyadi Suyadi Suyadi Suyadi Suyadi Syahruddin Said T. Susilawati T. Susilawati Tri Eko Susilorini Tri Eko Susilorini tri wahyuni Trinil Susilawati Triwik Sri Mulati Veronica Margareta Ani Nurgiartiningsih Wicaksana, Zulfikar Loka Widi Nugroho Widiasmoro, Ndaru Pranowo Wike andre Septian Wiranto Wiranto Yadi Malda Yayuk Fatmawati, Yayuk Yulian, Ria Zainuddin Zainuddin Zainuddin, Zainuddin Zainuri Sabta Nugraha, Zainuri Sabta Zhafirrahman, Muhammad Zulfaini Shamad