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All Journal HAYATI Journal of Biosciences Jurnal Ilmiah Farmasi EKSAKTA: Journal of Sciences and Data Analysis Natural Science: Journal of Science and Technology e-Journal Pustaka Kesehatan Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Pharmaciana: Jurnal Kefarmasian Indonesian Journal of Cancer Chemoprevention Indonesian Journal of Pharmaceutical Science and Technology Majalah Obat Tradisional INDONESIAN JOURNAL OF PHARMACY Universa Medicina Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology JURNAL MANAJEMEN DAN PELAYANAN FARMASI (Journal of Management and Pharmacy Practice) Majalah Farmaseutik JPSCR : Journal of Pharmaceutical Science and Clinical Research Jurnal Ilmu Kefarmasian Indonesia JKPK (Jurnal Kimia dan Pendidikan Kimia) JOURNAL OF COMMUNITY ENGAGEMENT AND EMPOWERMENT Nursing Care and Health Technology Journal (NCHAT) Khazanah: Jurnal Mahasiswa Malacca Pharmaceutics Indonesian Journal of Pharmacology and Therapy

Triana Hertiani

Faculty Of Pharmacy, Universitas Gadjah Mada

Author-ID : 3038040

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Skrining Senyawa Antiinfeksi dari Spons yang Dikoleksi dari Bunaken, Manado Puji Astuti; Gemini Alam; Sylvia Pratiwi; Triana Hertiani; Subagus Wahyuono
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 8, No 2 (2003): June 2003
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v8i2.2883

The emergence of drug resistant and multidrug-resistant microbes as well as the lack of any current chemotherapy augmented the necessity to search for new and better anti-infective drug.. This study was aim to screen potential antiinfective extracts of sponges collected from Bunaken, Manado and to report on their antibacterial and antifungal properties. Testing for antiinfective agents was conducted using dilution method. Nutrient Agar was used as the testing media and nutrient broth for the inoculation of microorganisms. Staphylococcus aureus, Escherichia coli and Salmonella typhi were used as the testing bacteria and Candida albicans as the testing fungi. Chloramphenicol was used as positive control for anti bacterial activity and Ketokonazol for antifungi. The two acetone extracts tested (MD-01aceton and MD-02aceton), all of them showed inhibition activities. Following partition with chloroform and methanol, all chloroform extracts inhibited the growth of both bacteria and fungi. These extracts then are considered to be potential candidates for further isolation and characterization as antiinfective agents.

Effect of different preparation techniques of red dragon fruit (Hylocereus polyrhizus) extracts on normal human fibroblast viability Novi Febrianti; Triana Hertiani; Sukarti Moeljopawiro; Sofia Mubarika Haryana
Pharmaciana Vol 9, No 2 (2019): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Red dragon fruit is one of the popular fruits that have been widely used both for consumption and food coloring. The red dragon fruit peel and flesh contain various antioxidant compounds that can be used as pharmaceutics and nutraceuticals. The objective of this study was to determine the effect of various extract preparations of the peel and the flesh of red dragon fruit on the viability of normal human fibroblasts. Seven conditions of peel and flesh extracts were prepared as follows, i.e. dried peel ethanolic extract, fresh blended peel ethanolic extract, dried flesh, fresh blended flesh ethanolic extract, blended fresh flesh, filtrate of pressed flesh, and pomace of pressed flesh. Each sample preparation was tested for its effect on the viability of normal human fibroblasts using MTT assay. Results showed that dried peel ethanolic extract reduce cell viability. Red dragon fruit flesh extracts caused no significant effect on the fibroblast viability. In conclusion, the fruit flesh extracts are relatively safer to normal cells than the peel extracts. IC50 value of the ethanolic extract of dried peelÂ was 55.38Â±3.85 Âµg/mL, while the IC50 value of various types of flesh extract were more than 500 Âµg/mL.

POTENSI ANTIMIKROBA EKSTRAK ETANOL SARANG SEMUT (Myrmecodia tuberosa Jack.) TERHADAP Candida albicans, Escherichia coli DAN Staphylococcus aureus Yuli Nurullaili Efendi; Triana Hertiani
Majalah Obat Tradisional Vol 18, No 1 (2013)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

The importance of finding a new natural source of antiinfective compounds has urged a research to explore antimicrobial activity of Ant-Plant (M. tuberosa) ethanol extract. This particular plant has been widely used in West Papua as part of traditional remedy. This research aimed to explore the antimicrobial potency of Ant-Plant ethanol extract against C. albicans, E. coli and S. aureus and to characterize the active compound group responsible for the activity. Dried powders were macerated in ethanol 70%, followed by evaporating the solvent. The extract was screened for antimicrobal activity against C. albicans, E. coli, and S. aureus by disc difussion method. A micodillution assay was performed to find out the MIC values, followed by MBC value determination in suitable solid media. Bioautography contact method was used to detect the antimicrobial active spots. The result revealed the extract’s MIC values against C. albicans, E. coli, and S. aureus were 0.8% w/v; 0.8% w/v; and 1.6% w/v respectively. MBC values were >6.4% w/v against C. albicans, 6.4% w/v against E. coli and 1.6% w/v against S. aureus. Active spot against E. coli and S. aureus shown by bioautography test results had hRf 0 (silica gel 60 F254, toluene: acetone: methanol: formic acid (26:8:5:1) v/v) while hRf 53 spot was active against C. albicans and S. aureus and detected as phenolic. It was concluded that the M. tuberosa ethanol extract contained active compounds which were potential to be developed as antimicrobial agent especially S. aureus.

DAYA ANTI QUORUM SENSING EKSTRAK DAUN KAYU MANIS (Cinnamomun burmannii Ness. Ex Bl.) TERHADAP Pseudomonas aeruginosa Fitri Apriliany; Hady Anshory; Triana Hertiani
Majalah Obat Tradisional Vol 18, No 3 (2013)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Quorum sensing is a communication system among bacterial cells which correlates with biofilm formation. Biofilm can protect bacteria from environment including antibiotic of which can cause higher antibiotic concentration of 100 up to 1000 times. Inhibition of quorum sensing is expected to inhibit the biofilm formation. The cinnamon bark (Cinnamomum burmanii Ness. Ex Bl.) has been known to have antibacterial and antibiofilm activities. Leaves are available abundantly which urges a research to find out the activity as anti quorum sensing against Pseudomonas aeruginosa. The succesive maceration of dried pulverized leaves produced hexane, ethyl acetate, and methanol extracts. Antibacterial activity was observed by microdillution method with MTT assay. Afterwards, the active extract was examined for anti quorum sensing activity by diffusion method in cetrimide Agar. Quorum sensing activity was shown by dark zone (opaque) growth around sample application, observed under UV light of 366 nm. TLC bioautography method was done to observe the active spots by using silica gel F254 as the stationary phase, chloroform-methanol (6:1 v/v) as the mobile phase, loading sample used was 1.25 mg and 30 min of plate contact duration. The ethyl acetate extract inhibited growth of P. aeruginosa with shown by MIC at 8 µg/µL Quorum sensing as well as growth inhibition activities were observed at loading samples 12,5 and 25 mg/wells, while at 6,25 mg the extract only exhibited quorum sensing inhibition. Presences of substances having phenolic, flavonoid, alkaloid and aldehyde/keton as functional groups were detected by TLC method of the extract but no active spot identified on bioautography.

OPTIMASI KOMPOSISI SUKROSA DAN ASPARTAM SEBAGAI BAHAN PEMANIS PADA FORMULA TABLET-EFFERVESCENT EKSTRAK ETANOLIK BUAH MENGKUDU Galih Pratiwi; Triana Hertiani; Mufrod Mufrod
Majalah Obat Tradisional Vol 16, No 2 (2011)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Mengkudu (Morinda citrifolia L.) has been widely used as traditional medicine. Its unpleasant smell and flavor urge a more acceptable dosage formulation. The aim of this research was to optimize the composition of sucrose and aspartame as sweetening agent in effervescent tablet formulation by using Simplex Lattice Design method. Effervescent tablets were produced by fusion method in five (5) different formulas, i.e. formula I (100% sucrose), II (suucrose-aspartame=75%:25%), III (sucrose-aspartame=50%:50%), IV (sucrose-aspartame=25%:75) dan V (100% aspartame). Effervescent granules were evaluated for mass density, flowing time, tapping index and compactibility characteristics. The effervescent tablets were tested for weight uniformity, hardness, friability, and disintegration time characteristics as well as TLC profile chromatogram. Data was analyzed by one way ANOVA, Scheffe method and Kruskall-Wallis with significance level 95%. The tablet acceptability was tested among 30 respondents. The results showed that the different composition of sucrose-aspartame influence the physical characteristics of granules and tablets effervescent produced. More sucrose content will increase the hardness, lower the friability but prolong the disintegration time. 70% respondents chose formula III as the best formula. Evaluation of SLD data recommended sucrose and aspartame in 42:58 proportion as the most optimum formula.

OPTIMASI KOMPOSISI ETANOL-AIR SEBAGAI CAIRAN PENYARI DALAM PRODUKSI EKSTRAK KERING DAUN SAMBUNG NYAWA [Gynura procumbens (Lour.) Merr.)] Tantri Lilis Nareswari; Triana Hertiani
Majalah Obat Tradisional Vol 21, No 1 (2016)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Sambung Nyawa leaves (Gynura procumbens (Lour.) Merr has been widely used as herbal medicine which requires a quality improvement of the dry extract for industrial production. Extraction solvent optimization is one key factor which determines the quality. This research aims was to find out the optimal ethanol-water composition as extraction solvent by using Simplex Lattice Design (SLD) method of which the total phenolics, total flavonoids and DPPH radical scavenging activity were used as quality parameters. Dried leaves as raw materials were pulverized and screened at Mesh 60, macerated (1:5) with ethanol-water compositition as 1:0; 0.7:0.3; and 0.5:0.5 v/v, shaked for 24 h, filtered. The procedure was repeated twice. Filtrates were collected of which lactose were added (1:2)w/w and spray dried at 100C for 30 min. Dried extracts yielded were evaluated the quality by using SLD method of which the total phenolics, total flavonoids as well as DPPH radical scavenging activity were used as parameters. Optimal SLD response was revealed at the ethanol:water composition of 0.66:0.34 – 0.75:0.25 v/v (Rtotal>0.9). No significant difference of the above mentioned parameters between the values resulted from the experiment and SLD formula. Correlation analyses of total phenolics and total flavonoids towards DPPH-radical scavenging activity were found as 95.29% and 1.25%, respectively.

AKTIVITAS ANTIRADIKAL DPPH SERTA PENENTUAN KANDUNGAN FENOLIK DAN FLAVONOID TOTAL SARI LARUT AIR DAUN DAN BUAH Ficus carica L. DAN Ficus parietalis Bl. Oktavia Tri Wahyuni; Triana Hertiani
Majalah Obat Tradisional Vol 21, No 2 (2016)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Ficus carica L and Ficus parietalis Bl. (Moraceae) are closely related plants which also known in Indonesia as Figs L. Considering the wide therapeutic value of Figs, this research was aimed to evaluate the DPPH-radical scavenging activity of both plants as well as their total phenolic and flavonoids. Extracts were produced by using boiled water and diluted to gain the desired concentration. Analyses were performed by using UV-vis spectrophotometer. Radical scavenging activity testing was done by using radical of DPPH (2,2-diphenyl-1-pycrylhydrazyl) to determine the IC50s. The determination of total phenolic was conducted by using Folin-Ciocalteau method and calculated as Gallic Acid Equivalence (GAE). The total flavonoid was measured by using AlCl3-reagents, and calculated as Rutin Equivalence (RE). Afterwards, the radical scavenging activity was correlated to the total phenolic and flavonoids contents. The results showed that the water soluble extract of F. carica fruit had the best IC50 value of 33.38 mg/mL, followed successively by the F. parietalis fruit (35.69 mg/mL), F. parietalis leaves (44.01 mg/mL) and the F. carica leaves (76.38 mg/mL). The highest content of total phenolic was shown by the leaves of F. parietalis (1.46% w/w GAE) and the lowest was in the fruit or F. carica (0.36% w/w GAE). The highest flavonoid content was detected in the leaves of F. carica (1.42% w/w RE) and the lowest was in the F. parietalis fruit (0.20% w/w RE). Correlation analyses of the IC50 values vs. the total phenolic and the flavonoids contents resulted in a positive slope having R2 values as 0.5362 and 0.9895, respectively. As a conclusion, the total flavonoid content influenced the DPPH radical scavenging activity by 98.95%, while the total phenolic content influence was only 53.62%.

Correlation Between Total Flavonoid Contents and Macrophage Phagocytosis Activity of Fractions From Faloak (Sterculia quadrifida R.Br.) Barks Ethanolic Extract In Vitro Rima Munawaroh; Siswadi Siswadi; Erna Prawita Setyowati; Retno Murwanti; Triana Hertiani
Majalah Obat Tradisional Vol 23, No 1 (2018)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

On Timor island, Nusa Tenggara Timur, faloak barks (Sterculia quadrifida R.Br.) has been used empirically to restore stamina. Faloak bark ethanolic extract proved to have immunomodulatory activity in vitro, which can increase macrophage phagocytosis activity. This research aimed: (i) to determine the immunomodulatory active fraction of faloak bark ethanolic extract, (ii) to determine the total flavonoid contents of faloak extract and fractions, and (iii) to evaluate the correlation of the total flavonoid contents of those extract and fractions with their macrophage phagocytosis activity. The simplisia powder is macerated with 96% ethanol. The extract was dissolved in methanol:water (9:1v/v) was then subsequently partitioned with n-hexane, ethyl acetate, and water to obtain n-hexane fraction, ethyl acetate fraction, water fraction, and insoluble fraction. Faloak extract and fractions at concentration 62,5; 125; 250; 500μg/mL were tested for their effect on the peritoneal macrophage phagocytosis of Balb/c mice in vitro by the latex beads method. Phagocytosis capacity and phagocytosis index were analyzed using one-way anova and post hoc Tukey HSD test with 95% confidence level. The results showed that ethyl acetate fraction had the highest macrophage phagocytosis capacity and the highest total flavonoid content compared to other fractions. The highest macrophage phagocytosis capacity of ethyl acetate fraction at concentration of 250 μg/mL was 51,94±4,67%, this value was significantly different from cell control (7,50±1,29%), negative controls of 0,0625% dimethylsulphoxide (6,25±0,36%), as well as positive control of 200 μg/mL echinaceae extract syrup® (9,97±0,33%). The total flavonoid content of ethyl acetate fraction determined by aluminum chloride method was 4,290±0.029 mg of quercetin equivalent/g fraction. There was a positive and strong correlation between the total flavonoid content of these extract and fractions with their macrophage phagocytosis capacity (Pearson correlation coefficient of 0,781) and showing linear relationship y=4,721x+19,663; R2=0,61.

The Inhibition Activity of Tannin on the Formation of Mono-Species and Polymicrobial Biofilm Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans Hasyrul Hamzah; Triana Hertiani; Sylvia Utami Tunjung Pratiwi; Titik Nuryastuti
Majalah Obat Tradisional Vol 24, No 2 (2019)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Biofilm acts as the mediator for infection nowadays. Approximately, more than 80% infection incidents are biofilm-formation related. Biofilm as bacteria's defense system is more difficult to eradicate by antibiotic; therefore, pathogen bacteria on their biofilm forms can make serious problems for human health. The invention of a new candidate for polymicrobial biofilm can be an essential challenge to be studied, in order to prevent infections related to biofilm. Tannin is a polyphenol compound with anti-bacterial and anti-fungal potential. This study aims to acknowledge the effectiveness of tannin in inhibition and degradation of C. albicans, P. aeruginosa, E. coli, S. aureus, and polymicrobial biofilm. The assay for biofilm inhibition and degradation were determined with microtiter broth method. The effectivity of tannin antibiofilm against polymicrobial biofilm were analyzed by calculating minimum biofilm inhibitory concentration (MBIC50) and minimum biofilm eradication concentration (MBEC50) values. The mechanism of action of tannin against polymicrobial biofilm was tested using scanning electron microscopy (SEM). The data were analyzed using the Statistical Package for the Social Sciences (SPSS) with a 95% confidence level. Tannin 1% gave inhibition activity of mono-species biofilm formation S. aureus in the middle phase and maturation of 79.04±0.01, 61.48±0.03, E. coli 74.56±0.01, 67.91±0.02, P. aeruginosa 67.32±0.05, 35.13± 0.01, C. albicans 60.62±0.01, 47.16±0.01. The results also provide evidence that tannin activity can degrade and damage the matrix of extracellular polymeric substance (EPS) polymicrobial biofilms. Hence, tannins can be a potential candidate for new antibiofilm for polymicrobial biofilm.

TOXICITY TEST OF MAKUTADEWA (Phaleria macrocarpa (Scheff.)Boerl.) BARK AGAINST Artemia salina Leach AND THIN LAYER CHROMATOGRAPHY PROFILE OF THE ACTIVE FRACTION Triana Hertiani; Silvia Utami Tanjung Pratiwi
Indonesian Journal of Pharmacy Vol 13 No 2, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Makutadewa (Phaleria macrocarpa (Scheff) Boerl.) is one of the herbal medicines used against cancer, however, the scientific basis of the activity still void. Daphne mezereum, the same family (Thymelaeaceae) to makutadewa has been proved to contain cytotoxic substances in its bark. Therefore, the aims of this research were to investigate the toxic effect of makutadewa bark against Artemia salina Leach. as the primary step to identify an anticancer activity and find out substances responsible to this activity. The material powder was extracted using Soxhlet apparatus with chloroform, followed by methanol and finally by distilled water. The toxicity of chloroform, methanolic and aqueous extracts were assayed against A.salina Leach. The chloroform extract (most active) with the LC50 of 29.6  0.14 g/ml was fractionated by vacuum liquid chromatography using wash benzene 100 % (a); wash benzene-ethyl acetate =20:1 (b); 15:1 (c); 10:1 (d); 5:1 (e); 2:1 (f) and chloroform-methanol 1:1 (g) as mobile phases. The active fractions against A.salina were E {combination of (e) and (f)} with LC50 of 106,9 g/ml and F (g) with the LC50 of 131,53 g/ml, hence less toxic than the original extract. The thin layer chromatogram profiles showed that E and F fractions contained terpenoid and alkaloid substances.Key words: toxicity test, (Phaleria macrocarpa (Scheff)Boerl.), bark

Co-Authors ., Sudarsono ACHMAD FUDHOLI Afandi, Karima Agustina, Ikra Agustinus Yuswanto Ahmad Marzuki Ainaini, Rizkia Aini Febriana Aini FebrianaÃƒÂ‚Ã‚Â Akhmad Khumaidi Ali Djamhuri Amalia, Latifa Ardani, Marisya Atikana, Akhirta Bawon Triatmoko Bayu Irawan, Muhamad Budi Pranoto Christin Marganingsih Santosa Dellyna Feronica Manik Dewi Setyaningsih Dian Adityaningrum Dwi Salim, Rozin EDIATI SASMITO Ediati Sasmito Edrada, Ru Angelie Eka Prasasti Nur Rachmani Elza Fadia Irzani Endang Setyowati Erma Yunita Erna Prawita Setyowati Erna Prawita Setyowati Fadilah Qonitah FERRY RAHMA PUSPITA Fitri Apriliany, Fitri Fransiska Leviana Galih Pratiwi Galih Pratiwi, Galih Gemini Alam Gemini Alam Hady Anshory Hady Anshory Hady Anshory Hamzah, Hasyrul Hanif Nasiatul Baroroh Harjanti, Kestri Indah Purwantini Irami Duma Kencana Irianto Iramie Duma Kencana Irianto Irawan, Muhamad Bayu Kartika, Senda Kestri Harjanti Khafi, Muhammad Khairan Khairan Kharismatul Khasanah Lay, Caterina Siskadewi LINDA SUKMARINI LISA SOEGIANTO Mahardika Agus Wijayanti Mahardika Agus Wijayanti Mahardika Agus Wijayanti Mahardika Agus Wijayanti Marchaban Marchaban Marchaban Marchaban, Marchaban Maria Ulfah Maulidia, Faiqoh Nur Michael Kubbutat Mistriyani, Mistriyani Mufrod Mufrod mufrod mufrod Muller, Werner E.G. Musthofa Mustofa Mustofa Mustofa Mustofa Mustofa Mustofa Narastika, Longina Ni Luh Putu Vidya Paramita Novi Febrianti NURI IRIYANI Nurul Jannah Oktavia Tri Wahyuni Pamungkas, Aryaningtyas Widya peter Proksch Peter Proksch Peter Proksch, Peter Pratama, Nofran Putra Pratiwi, Agrita Eka Pratiwi, Sekar Ayu Pratiwi, Sylvia Prayoga, Muhammad Bagas Proksch, Peter Puji Astuti Puji Astuti Purnomo, Kurnia Rahayu PUSPITA LISDIYANTI Puspita, Nanda Ayu Putri Indah Sayakti Putri, Faradhyta Maharani Rachel Turalely Rachel Turalely Rachel Turalely Rachel Turalely Rahmayani, Almira Rasydi, Ahmad Retno Murwanti Rica Rica Rica Rica, Rica Rima Munawaroh Rinaldi Idroes Rizkia Ainaini rob W.M. van Soest Rob W.M. Van Soest, Rob W.M. ROCHMAT MUDJAHID Rodiata, Tsania Andaya Ronny Martien Ru Angelie Edrada-Ebel RuAngelie Edrada RuAngelie Edrada, RuAngelie Rumondang, Amanda Sanan, Antonius Oenunu Sari R Sari R, Sari Sasmito, Ediati SEPTI WULANDARI Setiawan, Vania SHANTI RATNAKOMALA Silvia Utami Tanjung Pratiwi SINTAYU PUTRI WANDAN SARI Siswadi Siswadi Siswandi Siswandi, Siswandi Sobri Iskandar Sofia Mubarika Haryana Subagus Wahyuono Subagus Wahyuono Subagus Wahyuono Sudarsono . SUDIBYO MARTONO Sukarti Moeljopawiro Sumarni Sumarni Sumarni Sumarni Suryawati Suryawati SUWIJIYO PRAMONO Syamsu Nur, Syamsu Sylvia Pratiwi Sylvia Utami Tanjung Pratiwi Sylvia Utami Tunjung Pratiwi Tamhid, Hady Anshory Tantri Liris Nareswari Thoâ€™atun Ma'rufah Titik Nuryastuti Ulfa Afrinurfadhilah Darojati Untari, Febriana Van Soest, Rob W.M. Werner E.G. Muller Wiwin Herdwiani WOUTER L.J. HINRICHS Yolanda Yolanda Yolanda Yolanda, Yolanda YOSI BAYU MURTI Yosi Bayu Murti Yuli Nurullaili Efendi Yuli Nurullaili Efendi, Yuli Nurullaili Zahra Zahra Zahra Zahra Zulbayu, L.M. Andi Zullies Ikawati

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