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Perubahan Histopatologi Kulit Ikan Koi (Cyprinus carpio) yang Terinfestasi Ichthyophthirius multifiliis Secara Kohabitasi [Skin Histopathology Alteration of Koi (Cyprinus carpio) With Ichthyophthirius multifiliis Infested Accordance Cohabitation] Gunanti Mahasri; Lyla Wulandari; Kismiyati Kismiyati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 3 No. 1 (2011): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v3i1.11663

Abstract

AbstractKoi (Cyprinus carpio) is one of the most famous freshwater ornamental fish which offers financial advantage. There are a lot of factors which have to be face when we're trying to cultivate or culture Koi, one of those factors is the disease. Parasite which always in freshwater fishes is Ichthyophthirius multifiliis. I. multifiliis penetrated through skin causing destruction ephitelium cells by cilia and by consequent causing a histopathological changing. The objectives of this research was to determine alteration of Koi (Cyprinus carpio) skin histopathology which infested by I. multifiliis through cohabotation. Cohabitation was done during 4 days with mixing 7 fishes infected with white spot disease to 100 healthy fishes. Koi that had been infested observing of pathology changes. And than slicing the skin organ to prepare histopatology slide from 3 sample in every level infestation. To detect the level infestation, parasite was divide into 3 level respectivelly are low level (1-5 parasite/slide), the medium level (6-10 parasites/slide) and the heavy level (more than 11 parasites/slide). Furthermore, damage level determination by histopatology was decided by scoring. Data analyzed with descriptive based on clinical simptom, and than, the skin histopatology which had been attacked was done by scoring. The results would be analyzed statistical with Kruskal Wallis test. If it is show the significant defference, the test would be continued with Z test 5% (Siegel, 1986).   The research showed that the fish skin Koi infestation by I. multifiliis there was histopatology changed shaped respectivelly epidermis proliferation, infiltration of inflammation cell and ephithel erosion. Based on histopatological changed skoring caused by I. multifiliis infestation from various infestation grade, there were 62.2% of Koi fish skin was epidermis proliferasi, 73.3% of one was infiltration of inflammation cell and 28.9% ephithel erosion. Water quality measurement showed that temperature and acidity in normal condition, respectively otemperature between 24–26 C dan pH 8. 
Karakterisasi Protein Lernaea Cyprinacea Dengan Metode Elektroforesis SDS-PAGE [Characterization Of Protein Lernaea cyprinacea By Using SDS-PAGE Electrophoresis Method] Gunanti Mahasri; Ulia Fajriah; Sri Subekti
Jurnal Ilmiah Perikanan dan Kelautan Vol. 2 No. 1 (2010): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v2i1.11669

Abstract

Abstract One of the diseases that often attack are fish is parasitic disease caused by Lernaea cyprinacea, called Lernaeosis. This ectoparasites could be detected on skin, gill, eyes, fin or inside of mouth and nostril of fishes. The aim of the study was to know the character is tic of L.cyprinacea protein based on molecular weight using SDS-PAGE. The results of this study were expected as Lernaeosis vaccine candidate. The experiment used SDS-PAGE with 12% separating gel, 3% stacking gel, voltage 110 volts 28 A and gel staining using coomassie blue. The result showed that, there were eight protein bands with molecular weight 82.3, 73.3, 66.6, 60.5, 54.9, 27.5, 23.1 and 19.8 kDa. Among them, the bands with molecular weight 27.5, 23.1 and 19.8 kDa were thicker and clearer than the others.
Pengaruh Suhu Penyimpanan Terhadap Kerusakan Spora Myxobolus koi [The Effect of Storage Temperature in Myxobolus koi Spore Damage] Gunanti Mahasri; Titom Gusmana Putra Perdana; Kusnoto Kusnoto
Jurnal Ilmiah Perikanan dan Kelautan Vol. 11 No. 1 (2019): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v11i1.12270

Abstract

AbstrakSpora Myxobolus koi dapat mengalami kerusakan apabila disimpan dalam kondisi penyimpanan yang kurang baik. Penelitian ini bertujuan untuk mengetahui pengaruh suhu penyimpanan terhadap kerusakan spora Myxobolus koi serta untuk mengetahui suhu optimum untuk penyimpanannya. Penelitian ini dilakukan dengan metode rancangan acak lengkap (RAL) sebagai rancangan percobaan. Perlakuan yang digunakan adalah penyimpanan spora Myxobolus koi pada suhu kamar (28-34) oC, Refrigerator (2-4) oC, dan Freezer (-5 hingga -10) oC, dengan ulangan sebanyak 6 kali. Penyimpanan ini dilakukan selama 30 hari. Parameter utama yang diamati adalah prosentase spora Myxobolus koi yang rusak. Parameter penunjang yang diamati adalah tipe kerusakan spora Myxobolus koi. Analisis data menggunakan ANAVA (Analisis Varian) dan dilanjutkan dengan Uji Jarak Berganda Duncan untuk mengetahui suhu optimum untuk penyimpanan spora Myxobolus koi. Berdasarkan hasil penelitian diketahui bahwa peyimpanan pada suhu yang berbeda berpengaruh terhadap prosentase kerusakan spora Myxobolus koi. Kerusakan spora Myxobolus koi tertinggi terjadi pada suhu kamar (28-34) oC mencapai angka 68,91% dan nilai kerusakan terrendah terjadi pada refrigerator (2-4) oC yaitu 29,91%. Spora Myxobolus koi dapat disimpan pada refrigerator dan lemari pembeku. AbstractMyxobolus koi spores can be damaged if stored in poor conditions. This study aimed to determine the effect of storage temperature on Myxobolus koi spores and to determine the optimum temperature for storage. This research was conducted using a completely randomized design method (CRD) as an experimental design. The treatments used were storaged Myxobolus koi spores at room temperature (28-34oC), Refrigerator (2-4oC), and Freezers (-5 to -10oC), with replications 6 times. This storage was carried out for 30 days. The main parameter observed was the percentage of damaged Myxobolus koi spores. The supporting parameters observed were the type of Myxobolus koi spore damage. Data analysis using ANAVA (Analysis of Variance) and continued with Duncan's Multiple Distance Test to find out the optimum temperature for storage of Myxobolus koi spores. Based on the results of the study, it was found that storage at different temperatures affected the percentage of damage to Myxobolus koi spores. The highest damage of Myxobolus koi spores occurred at room temperature (28-34oC) reaching 68.91% and the lowest damage value occurred at refrigerator (2-4oC) which was 29.91%. Myxobolus koi spores can be stored in a refrigerator and freezer
Correlation of Nitrite and Ammonia with Prevalence of Enterocytozoon hepatopenaei (EHP) in Shrimp (Litopenaeus vannamei) on Several Super-Intensive Ponds in East Java, Indonesia Anord Charles Nkuba; Gunanti Mahasri; Nunuk Dyah Retno Lastuti; Adamu Ayubu Mwendolwa
Jurnal Ilmiah Perikanan dan Kelautan Vol. 13 No. 1 (2021): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v13i1.24430

Abstract

AbstractEnterocytozoon hepatopenaei (EHP), a microsporidia parasite known to hinder shrimp growth by infecting its hepatopancreas, is recently an emerging infection for Litopenaeus vannamei farms in Indonesia. The present study was conducted to investigate the relationship between nitrite and ammonia concentration with EHP infection in the super-intensive ponds. Shrimps and water samples were collected from six ponds in aquaculture super-intensive ponds with a stocking density of 5,000 shrimps in each pond which is located in Pasuruan, Lamongan, and Tuban Regency, Indonesia. The water sample was taken to the laboratory for the measurement of ammonia and nitrite. The samples of ammonia and nitrite were detected by spectrophotometer, and the PCR was used to detect 18S rRNA of EHP. The PCR analysis that showed two positive samples of L. vannamei, from the ponds with a high concentration of ammonia and nitrite, were infected by EHP. The statistical analysis showed a significant correlation between ammonia and nitrite with the prevalence of EHP infections, where the Pearson correlation (r) was 0.980 and 0.943, respectively. There was a high prevalence of EHP infection with the increase in nitrite concentration and ammonia in pond four and pond six. The concentration of over 1mg/l of ammonia and nitrite could influence EHP infection prevalence in the shrimp farms. Highlight ResearchAmmonia and nitrite concentration influence the prevalence of Enterocytozoon hepatopenaei infection in Super intensive ponds.The estimated prevalence of Enterocytozoon hepatopenaei was 16.7%.PCR amplification analysis was used to detect the 18S ss-rRNA of Enterocytozoon hepatopenaei and 510-bp was obtained.
The Effect of Immersion Duration of Zoothamnium penaei Crude Protein Extracts to Stimulate Immune System in Litopenaeus vannamei Against Enterocytozoon hepatopenaei (EHP) Anord Charles Nkuba; Gunanti Mahasri; Nunuk Dyah Retno Lastuti; Adamu Ayubu Mwendolwa
Jurnal Ilmiah Perikanan dan Kelautan Vol. 15 No. 2 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v15i2.38349

Abstract

Highlight Research The effect of immersion duration of shrimp to the immunostimulant from crude protein Zoothamnium penaei was determined. 20-minute immersion duration in crude protein from Zoothamnium penaei was found to increase the phagocytosis activities, phenoloxidase, and improve hepatopancreases of the shrimp. There is increase in phagocytosis activities, phenoloxidase and improved hepatopancreases cell within a three-week maintenance of shrimp with immersion after each seven days. There is interaction between immersion duration and maintenance time of shrimp into the crude protein from Zoothamnium penaei. Abstract Enterocytozoon hepatopenaei (EHP) is a spore-forming microsporidia intracellular parasite, which slough off the cells of the hepatopancreases of the shrimp. This study was intended to determine the effect of immersion duration and maintenance time to stimulate the immune system of shrimps by using a crude protein from Zoothamnium penaei to protect against EHP infections. The study used a completely randomized design where shrimps were divided into four groups each having five replicates. Groups P1, P2, P3 were immersed in immunostimulant for 10, 15, and 20 minutes respectively, except negative control. The phenoloxidase, phagocytosis activities, histopathology study were determined. Also, the molecular diagnosis of EHP was measured to confirm EHP infection in the shrimp samplesThe phenoloxidase activity significantly increased after immersion for 20 minutes (P3) from 0.64-, 0.72-, and 0.78-units min-1 mil -1 of protein on the first week, second week and third week, respectively. The phagocytosis activities of the sample immersed for 20 minutes were significantly increased to 68% on the second week. The sample also showed improved hepatopancreases epithelial cells with only 26% necrotic cell, less EHP spore and vacuolation. There was an interaction between immersion duration and maintenance time of the L. vannamei immersed in crude protein from Zoothamnium penaei. Twenty minutes immersion were significantly found to stimulate the immune system of the shrimp against EHP. The present work revealed that, the application of crude protein from Z. penaei is effective against EHP in shrimp culture.
ADDITION OF Caulerpa racemosa EXTRACT ON RPS AND CLINICAL SIGNS OF VANAME SHRIMP AFTER Vibrio parahaemolyticus INFECTION Muhammad Hilmy Maulana; Woro Hastuti Satyantini; Gunanti Mahasri
Jurnal Biosains Pascasarjana Vol. 25 No. 2 (2023): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v25i2.2023.79-89

Abstract

Prevention of AHPND in shrimp can be done by giving immunostimulants, one of which comes fromseaweed extract, explicitly Caulerpa racemosa. This study aimed to determine the effect of the administration of Caulerpa racemosa extract on the relative percent survival (RPS), clinical signs, and behavioral changes of white shrimp after being infected with Vibrio parahaemolyticus. This study used a completely random design with five treatments and three replications, namely K-, K+, and three treatments with the addition of Caulerpa racemosa extract, names P1 (3 μg/g), P2 (6 μg /g), and P3 (9 μg/g). The infection process with Vibrio parahaemolyticus bacteria was carried out 24 hours after being injected with Caulerpa racemosa extract. The results of this study indicate that the highest Relative Percent Survival (RPS) value was P3. Clinical signs of shrimp infected with Vibrio parahaemolyticus were characterized by empty intestines and a whitened hepatopancreas. In addition, there were behavioral changes in shrimp infected with Vibrio parahaemolyticus, namely decreased appetite, passive shrimp movement at the bottom of the aquarium, and erratic swimming movement of shrimp. Based on these results, it could be said that the best treatment is in P3 with a dose of 9 μg/g. This study can provide the best quantity of C. racemosa extract to treat AHPND in shrimp.
THE EFFECT OF TRANSPORTATION ON BLOOD GLYCOSE LEVELS AND ECTOPARASITE INVESTMENT IN PUNTEN CARP (Cyprinus carpio) Wiwin Sumiati; Gunanti Mahasri; Sri Subekti
Jurnal Biosains Pascasarjana Vol. 25 No. 2 (2023): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v25i2.2023.128-136

Abstract

Carp is one of the most popular sources of animal protein. Aquaculture products must be distributed through a transportation system to meet the demand for carp. Improper transportation techniques will cause the condition of the fish to deteriorate, thereby increasing stress, the parasites susceptible enter the fish's body. The aim of this study was to determine the effect of transportation on blood glucose levels and ectoparasite infestation in Punten carp. The method used in this study is an experimental method with descriptive approach by comparing the values of blood glucose levels and parasite infestations in fish before the transportation process and after the transportation process. The results of the analysis of water quality before the transportation and after the transportation process still within normal limits. In the transportation treatment, Mas Punten fish can increase blood glucose levels significantly with a value before transportation of 31.54 mg/dL and after transportation of 51.41 mg/dL. The increase in ectoparasite survival was not significant with an average number of 10.64 ind/head (medium) before transportation and 12.08 ind/head (moderate) after the transportation process. There is a correlation between blood glucose levels and ectoparasite infestation with the degree of correlation. Based on the chi test, it was found that the treatment of the Punten Mas fish transportation system can affect the blood glucose level of fish very significantly (sig> 0.01), but it is not associated with an increase in ectoparasite infestations that occur (.338>0.01).
Pathology Anatomy and Hemolymph Profile of Spiny Lobster (Panulirus homarus) Infested by Octolasmis sp. on Bottom Cage Bahtiar, Sadida Anindya; Mahasri, Gunanti; Ulkhaq, Mohammad Faizal
Jurnal Medik Veteriner Vol. 7 No. 1 (2024): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss1.2024.57-67

Abstract

Spiny lobster, a fishery commodity with important economic value, is often infested by Octolasmis sp. on their bodies, which causes stress conditions that lead to death. This study aimed to determine the anatomical pathology and hemolymph profile of spiny lobster infested with Octolasmis sp. in bottom cages. The method used was an observation, where 30 lobsters (weight 112.44 ± 24.63 g) were carried out in June–July 2022 at the Pesona Bahari Wongsorejo, Banyuwangi. Hemolymph profile and anatomical pathology data were analyzed, respectively, using the Independent T-Test and Kruskal-Wallis test (α = 95%). The results based on scoring data showed a very significant difference between infected and healthy lobsters (p < 0.01), characterized by the occurrence of tail flakes, lesions on the carapace, gill melanization, and the appearance of ulcers on the ventral abdomen. A very significant change occurred in the hemolymph profile of infected lobsters compared to healthy lobsters (p < 0.01). Total hemocyte count increased with high concentrations of granular cells, respectively 22.1–37.8 ´x 106 cells/mL and 63.75–64.5%, along with lobster damage, while hyaline cells decreased. This study showed that there was a change in anatomic pathology and hemolymph profile between infected and healthy lobsters.
Identification of Morphometric and Intensity of Marine Leech Zeylanicobdella Infestation on Cantang Grouper from Brondong District, Lamongan and Mandangin Island, Madura Ririn Agustiya; Mahasri, Gunanti; Subekti, Sri
Journal of Marine and Coastal Science Vol. 12 No. 1 (2023): FEBRUARY
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmcs.v12i1.38273

Abstract

Cantang grouper (Epinephelus fuscoguttatus lanceolatus) is the result of hybridization between female tiger grouper and male grouper fish which is widely cultivated because it has fast growth, easy to breed, and lower cannibalism. Cultivation of cantang grouper can be carried out in semi-intensive ponds and floating net cages (KJA) which have the potential for grouper nursery and rearing activities. One of the problems in the cultivation of cantang grouper is the presence of disease attacks caused by marine leeches Zeylanicobdella with a prevalence of up to 100% in grouper culture in floating net cages. This study aims to determine differences in species and intensity of marine leech Zeylanicobdella infestation in cantang grouper (Epinephelus fuscoguttatus lanceolatus) from semi-intensive ponds in Brondong District, Lamongan and floating net cages (FNC) on Mandangin Island, Madura. The method used in this research is a survey and sampling is done by purposive sampling method. Cantang groupers measuring 15-20 cm from semi-intensive ponds and 20-25 cm from floating net cages each were taken as many as 35 individuals. Identification of Zeylanicobdella marine leeches using a binocular microscope and making sketches of ectoparasites with the help of a binocular microscope equipped with a lucida camera. Marine leech species found to infest the cantang grouper (Epinephelus fuscoguttatus lanceolatus) is Zeylanicobdella arugamensis which was found on the body surface, fins, gills, and eyes. The results of statistical analysis using the Mann-Whitney U test showed that there was no significant difference (p>0,05) between species and intensity of Zeylanicobdella arugamensis in cantang grouper reared in semi-intensive ponds and floating net cages. The intensity of marine leeches Zeylanicobdella arugamensis in semi-intensive ponds was in the medium category (14,74 individuals/head) and in floating net cages was in the medium category (27,74 individuals/head). The highest intensity of infestation marine leeches Zeylanicobdella arugamensis in floating net cages of 27,74 individuals/head. There was no significant difference in the intensity of Zeylanicobdella arugamensis marine leech infestation in cantang grouper both from semi-intensive ponds and floating net cages (FNC) which were included in the medium category.
Bacteria Detection Salmonella sp. in Fish Meatball Products Sold in Some Traditional Markets in Surabaya Permata Jelita, Chelsea; Mahasri, Gunanti; saputra, Eka
Journal of Marine and Coastal Science Vol. 12 No. 2 (2023): JUNE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmcs.v12i2.44921

Abstract

Fish meatballs are processed fishery products that use minced fish meat or surimi of at least 40% mixed with flour, and other ingredients if needed, which undergo formation and cooking. In general, fish meat used is for example snapper, grouper and mackerel. This study aims to determine the detection of Salmonella sp. bacteria, fish balls sold in several traditional markets in the city of Surabaya and the calculation of Total Plate Count of processed fish balls circulating in several traditional markets in Surabaya. The research method used is a direct survey for sampling. The sampling location of fish balls was done intentionally or purposive sampling method. Analysis of the data to determine the Total Plate Count of Salmonella sp. bacteria in fish balls in several traditional markets in Surabaya, which is displayedin tabular form. The results showed that the detection of Salmonella sp. bacteria was positive in two samples out of a total of 10 samples. The results of the Total Plate Count (TPC) test for Salmonella sp. in the two different samples in the same area of West Surabaya, it was 2x105 CFU/gr for the first sample and 1x103 CFU/gr for the second sample.
Co-Authors A. Shofy Mubarak Abdul Manan Abdul Manan Abyan Farras Adamu Ayubu Mwendolwa Adamu Ayubu Mwendolwa Adiacahya, Eren Aditya Gita Rohmatullah Adriana Monica Sahidu Agung Pamuji Rahayu Agus Nazarudin Yahya Ahmad Shofy Mubarak Akhmad Taufiq Mukti Alanosi Noor Muhammad Alfan Prianggara Alfin Tauhid Alim Isnansetyo Almira Fardani Lahay Alvira Febrianti Pratiwi Anam, M Khairul Anggun Khoirun Nikmah Anggun Nurani Citrowati Anisa, Hosia Anord Charles Nkuba Apri Supii Ardilas Heryamin Arika Juniarsih Arya Witantama Bahtiar, Sadida Anindya Berliana A Bernathdo Mahendra Robbi Putra Bidayatul Afifah Boedi S. Rahardja Boedi Setya Rahardja Browijoyo Santanamurti Cintia Larasati DARMAWAN SETIA BUDI Desak Ketut Sekar Cempaka Putri Dewi, Nina Nurmalia Dheani Nur Ahadya Syifa Chaerunissa Dieswinta Hardika Aris dika dika Dita Wisudyawati Eka Saputra Elangga Sony Widiharsono Elisabeth Benita Indarmastuti Endah Sih Prihatini Endang Dewi Masithah Era Insivitawati Ewang Mahendra Putra Fahdi Putra Utama Faisol Mas&#039;ud Faisol Mas’ud Farid Nur Salim Farizka Vinka Trinendyah Ferry Dwi Firmansyah Liananda Firly Waliani Rahma Fuad Fuquh Rahmat Shaleh Gian Suryanatha Hartawan Hari Suprapto Heru Suryanto Ika Purnamasari Ika Putri, Lia Oktavia Ikmalia A Ikmalia Amali Indah Hidayati Imani Iqbal Ghazali Irvansyah Irvansyah Isroni, Wahyu Kadek Racmawati Karunia, Fitria Kenconojati, Hapsari Kismiyati , Koesnoto Koesnoto Kusnoto Kusnoto Lailatul Lutfiyah, Lailatul Laksmi Sulmartiwi Laksmi Surmartiwi Larasati, Anastasya Dewi Lestari Wilujeng Lia Oktavia Ika Putri Lia Oktavia Ika Putri Lilis Cahaya Septiana Linnya Prima Agustin Lucia Tri Suwanti, Lucia Tri Luthfiana Aprilianita Sari Lyla Wulandari M Ervany Eshmat M Iqbal Satria Mandele, M. Muhtar Mayangsari, Cholivia Melinda Kusuma Ningrum Miftakhul Munir Moch Saad Mochammad Dwi Hardhianto Mohamad Yusuf Mohammad Faizal Ulkhaq Mufasirin Muhamad Amin Muhamad Amin Muhammad Amin Muhammad Arief Muhammad Herman Muhammad Hilmy Maulana Muhammad Musa N. Juni Triastuti Nafis Putra Laksana Cholil Nanuk Qomariyah Nedi Nedi Netty Sreani Nico Rahman Caesar Niluh Suwasanti Norma Isnawati Nugroho, Sefilia Adhi Nunuk Dyah Retno Lastuti Nur Fais Nurlita Abdulgani Nurul Kumalasari Ochtavia, Sherly Permata Jelita, Chelsea Prayogo Prayogo Pristita Widyastuti Puguh Yugo Wijanarko Putra, Bernathdo Mahendra Robbi Putri, Desak Ketut Sekar Cempaka Rachma Woro Anggarani Racmawati, Kadek Rahayu Kusdarwati Rahayu Kusdarwati Ridwansyah Ririn Agustiya Riris Ulfiana Riza Aryani Romziah Sidik Rozi Rozi Rr. Juni Triastuti Salman Aldo Alfaresi Samara, Syifania Hanifah Santanumurti, Muhammad Browijoyo Sapto Andriyono Saputra, Alfian Rahmadhani Satria Hani Sari, Putri Desi Wulan Saroso, Heri Setiawan Koesdarto Shandy Sulistyoningrum Sherly Ochtavia Shohifah, Isnatul Umu Siti Hamidah Sri Mulyati Sri Mulyati Sri Subekti Sri Subekti Sri Subekti Sudarno Sudarno Sudarno, Sudarno Sulistyoningrum, Shandy Suwasanti, Niluh Suzanita Utama Titom Gusmana Putra Perdana Ulia Fajriah Veryl Hasan W. Angan Indrawan Widodo, Langgeng Wijanarko, Puguh Yugo Wiwin Sumiati Wiwin Sumiati Woro Hastuti Satyantini Woro Hastuti Setyantini Wurlina, W Yanuhar, Uun Yudi Cahyoko Yunifar Amad