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Gambaran Histopatologi Kulit Ikan Kerapu Cantang (Epinephelus fuscoguttatus x Epinephelus lanceolatus) yang Terinfestasi Zeylanicobdella arugamensis Setelah Perendaman Menggunakan Perasan Daun Pepaya (Carica papaya) Bernathdo Mahendra Robbi Putra; Gunanti Mahasri; Laksmi Sulmartiwi
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.10575

Abstract

Increasing cultivation activities and intensifying ponds has the potential to give rise to problems, namely increasing cases of disease in grouper fish, namely Zeylanicobdella, causing mass deaths of up to 100%. This study aims to analyze the histopathological picture of grouper skin infested with Zeylanicobdella arugamensis after administering papaya leaf juice with different soaking times and concentrations of papaya leaf juice. The method used was experimental Completely Randomized Design (CRD) with a factorial pattern consisting of two factors, namely factor A, different concentrations of papaya leaf juice consisting of 0% (A1), 0.1% (A2), 0.3% (A3) , and 0.5% (A4), as well as factor B for grouper rearing time consisting of the 0th minute (B1), 60th minute (B2), and 120th minute (B3) with five repetitions. The results of the study showed that the soaking time of papaya leaf juice in grouper fish had an effect on reducing the degree of Zeylanicobdella infestation, there were changes in the histopathological picture and water quality values before and after soaking. Treatments A2, A3, and A4 experienced a decrease in the degree of ectoparasite infestation at 60 and 120 minutes of immersion. The histopathological scoring values for treatments A2, A3, and A4 decreased at 60 and 120 minutes of maintenance. The best treatment combination was 0, 3% and soaking time at 120 minutes. In conclusion, giving papaya leaf juice with a soaking time of 120 minutes with a concentration of 0.3% can reduce the degree of infestation and the histopathological scoring value of Zeylanicobdella in grouper fish.
Kekerabatan Zeylanicobdella arugamensis pada Ikan Kerapu Cantang dengan Pendekatan Molekuler di Jawa Timur dan Lombok Sherly Ochtavia; Gunanti Mahasri; Mufasirin Mufasirin
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.10627

Abstract

Giant grouper is a highly sought-after cultivated fish in Indonesia due to its high market value. One of the challenges in grouper aquaculture is the infestation of the sea leech ektoparasite Zeylanicobdella arugamensis. The prevalence rate of ektoparasite infestation reaches up to 100%. An effective method to manage ektoparasite infestation is through molecular profiling, serving as preliminary literature in the development of molecular biology efforts to overcome cultivation challenges in giant grouper. This research aims to study the homology and relationship of Zeylanicobdella arugamensis on giant grouper in East Java, specifically in Lamongan, Mandangin, Situbondo, and Lombok. The method used is RT-PCR with mtDNA COI gene primers. Molecular sequence data were collected from samples and compared with data from NCBI GenBank, and analyzed using Neighbor-joining phylogenetic tree method and homology data analysis on BLAST. The research results indicate clinical symptoms in giant grouper including swelling, lesions/wounds, pale skin, decreased appetite, and fin erosion. Electrophoresis results from PCR products show a single band at 750bp for samples from Lamongan, Situbondo, Mandangin, and Lombok. DNA sequencing results on NCBI BLAST and homology analysis against the reference gene were conducted. Homology analysis results between samples from Situbondo and Lamongan show the highest similarity at 98.28%, whereas the lowest similarity is between samples from Mandangin and Situbondo at 88.51%. Based on phylogenetic tree analysis, samples from Lamongan and Situbondo waters show a close relationship with a genetic distance of 0.009272.
PENDAMPINGAN MANAJEMEN KUALITAS AIR DAN APLIKASI PROBIOTIK PADA BUDIDAYA IKAN LELE PONDOK DARUL MUKMIN, SUKOREJO, PASURUAN JAWA TIMUR Nina Nurmalia Dewi; Woro Hastuti Satyantini; Wahyu Isroni; Gunanti Mahasri; Abdul Manan; Yudi Cahyoko; Dita Wisudyawati
RAMBIDEUN : Jurnal Pengabdian Kepada Masyarakat Vol. 7 No. 3 (2024): Rambideun: Jurnal Pengabdian Kepada Masyarakat
Publisher : LPPM Universitas Al Muslim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51179/pkm.v7i3.2955

Abstract

Pasuruan Regency is one of the regencies that has potential in the fisheries sector, especially freshwater fisheries cultivation. The potential area for cultivation activities is located in Sukorejo District with the cultivated commodity being catfish. One of the farmer groups in Sukorejo District is the Pondok Darul Mukmin Sangkuriang Catfish farmer. However, this catfish cultivation activity has not been running optimally, because the cultivation system used is still traditional. This community service activity aimed to provide assistance related to water quality management and probiotic application in catfish cultivation. This activity was carried out at Pondok Darul Mukmin from July to September 2024. The method of implementing the activity was in the form of training, assistance, and demonstrations of water quality management, probiotic application in feed, and probiotic culture for catfish cultivation. The results of this community service activity showed that Pondok Darul Mukmin fish farmer  were very enthusiastic in participating in the training activities. In addition, the community can also carry out catfish cultivation by monitoring water quality and applying probiotics to feed. Cultivation activities resulted in increased catfish growth every week.
PEMBERDAYAAN SISWA-SISWI SEKOLAH MENENGAH KEJURUAN NEGERI PERIKANAN DALAM PEMBENIHAN IKAN AIR TAWAR Akhmad Taufiq Mukti; Muhamad Amin; Putri Desi Wulan Sari; Veryl Hasan; Gunanti Mahasri; Puguh Yugo Wijanarko; Lia Oktavia Ika Putri; Salman Aldo Alfaresi
Jurnal Penamas Adi Buana Vol 8 No 01 (2024): Jurnal Penamas Adi Buana
Publisher : LPPM Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/penamas.vol8.no01.a8740

Abstract

The limited knowledge and skills in the hatchery technology application of the freshwater fish to seed production business are obstacles faced by prospective young entrepreneurs in the aquaculture sector. The Community Partnership Program (PKM) was provided as a strategic step to overcome the obstacles faced by the target partner group in this PKM activity in line with the Public Vocational Secondary School's (SMKN) expectations to provide sufficient insight into science and technology for SMKN students as provision for independent entrepreneurship. The aim of this PKM was to transfer the application of freshwater fish (catfish) hatchery technology and the produce fish seeds independently. The activity method was conducted through: a) delivery of material and training related to broodstocks selection and gonad maturation, b) training on the application of freshwater fish hatchery technology (catfish), and c) regular monitoring and assistance to PKM target partner groups by involving related agencies (SMKN). Delivery of material was carried out regularly, both online and face to face (offline). Approximately 40 students of SMKN Ngadirojo, Pacitan Regency who took the flagship program of Aquaculture Agribusiness, attended this activity. This PKM activity was also attended by accompanying teachers. The practical results show an increase in the knowledge and skills of partners in catfish hatchery through the application of semi-artificial catfish broodstocks spawning technology.
CAPTURE RESULTS OF BABY LOBSTERS (Panulirus sp.) LANDED FROM PACITAN COASTAL WATERS Andriyono, Sapto; Wijanarko, Puguh Yugo; Mahasri, Gunanti; Saroso, Heri
Journal of Aquatropica Asia Vol 10 No 1 (2025): Journal of Aquatropica Asia
Publisher : Program Studi Akuakultur, Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/joaa.v10i1.6362

Abstract

Lobsters from the Panulirus sp. group are a potential resource in renewable fisheries, but when exploitation continues to increase and there is no control, the number may continue to decline and become extinct. Lobster catching is not limited to the adult phase, but in all phases, including the puerulus phase (baby lobster/clear lobster seed). This study aimed to determine the diversity of baby lobster seeds landed in coastal waters in the Pacitan Regency area based on baby lobster catching activities. This research was conducted in January 2023 - January 2024. Data processing using Primer7 and MS Excel software. Based on the results of observations and data collection, both primary and secondary, 4 lobster species were obtained, namely pearl lobster (Panulirus ornatus), sand lobster (Panulirus homarus), bamboo lobster (Panulirus versicolor), and batik lobster (Panulirus longipes). Sand lobster, pearl lobster, batik lobster, and bamboo lobster were the most abundant at each station. During sampling, the average value of monthly catches in 2024 decreased compared to 2023. Water quality in this area is suitable for lobster habitat, with DO 8-8.5 ppm, salinity 33-35 ppt, pH 7-7.2, and temperature 27.0- 29.2 °C.
Collagen Extraction from Red Snapper (Lutjanus malabaricus) Scales via Hydroextraction: Toward Nanocollagen Development Mayangsari, Cholivia; Mahasri, Gunanti; Shofy Mubarak, Ahmad
Journal of Marine and Coastal Science Vol. 14 No. 2 (2025): JUNE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmcs.v14i2.72271

Abstract

Generally, collagen is isolated using acid, base and enzymatic hydrolysis methods. However, this has the constraint of the length of time for the release of procollagen to produce collagen. Acetic acid hydrolysis combined with the hydroextraction method is one alternative to produce quality collagen. The use of acetic acid in the extraction of red snapper scales (Lutjanus malabaricus) will produce H+ ions to release procollagen in the raw material. The study aims to evaluate the potential of red snapper (L. malabaricus) scales as a collagen source through the hydroextraction method for the development of nanocollagen formulations. This study used a completely randomized design (CRD). Isolation of red snapper scales collagen using acetic acid solvent concentrations of 1 M; 1.5 M and 2 M with a hydrolysis time of 2 hours and hydroextraction for 2 hours with three repetitions. The parameters observed in this study were the proximate of collagen raw materials, collagen yield, and collagen amino acids. The data analysis results were tested statistically using Analysis of Variance (ANOVA) followed by Tukey's further test. The best result of collagen isolation from red snapper (L. malabaricus) scales was achieved using 2 M acetic acid hydrolysis combined with hydroextraction. This study demonstrated that the combination of different acetic acid concentrations with hydroextraction had a significant effect (P<0.05) on collagen yield parameters. The amino acid content consisted of 22.37% glycine and 12.45% proline. These findings indicate that red snapper (L. malabaricus) scales have the potential to be developed as a raw material for nanocollagen developments.
Potensi Ekstrak Tanaman sebagai Antibakteri terhadap Vibrio parahaemolyticus: Sebuah Tinjauan Pustaka Anisa, Hosia; Amin, Muhamad; Mukti, Akhmad Taufiq; Mahasri, Gunanti
Jurnal Perikanan Unram Vol 15 No 4 (2025): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v15i4.1692

Abstract

Aquaculture faces serious threats, one of which is infectious disease caused by Vibrio parahaemolyticus bacteria. These bacteria have shown antibiotic resistance and cause significant economic losses. The need for safe and sustainable antibacterial alternatives is crucial given the negative impact of using synthetic antibiotics. This review explores the potential of Shorea beccariana, Uncaria gambir, and Swietenia macrophylla as natural antibacterial agents against Vibrio parahaemolyticus. A systematic descriptive study method was used to integrate primary literature. Results showed that V. parahaemolyticus is a pathogenic Gram-negative bacterium with a complex virulence mechanism. Various studies have confirmed the antibacterial activity of plant secondary metabolites, such as flavonoids, tannins, and terpenoids, against Vibrio spp. Although the specific effectiveness of these three plants against V. parahaemolyticus has not been widely studied, the bioactive compounds identified in their extracts show strong potential. These compounds work by damaging the integrity of bacterial cells and disrupting their metabolism, offering a promising natural solution for vibriosis control.
Correlation of Water Quality with OCR and SOD of Humpback Grouper in Net Cages in Concrete Ponds with Different Stocking Densities Nugroho, Sefilia Adhi; Gunanti Mahasri; Abdul Manan
Grouper Vol. 16 No. 2 (2025): Grouper : Jurnal Ilmiah Perikanan
Publisher : Universitas Islam Lamongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30736/grouper.v16i2.365

Abstract

The humpback grouper (Cromileptes altivelis) is one of the high-value commodities in Indonesia. Humpback grouper production from the cultivation sector has not been able to reach the expected production volume. The Floating Net Cage method is the latest technology in humpback grouper cultivation, but obstacles from weather and environmental factors are very large. The author is interested in conducting research on the stress levels of humpback grouper cultivated in the innovation of net cage cultivation media placed in concrete ponds with different stocking densities. The results showed that water quality parameters were no longer the main factor causing stress in fish raised in net cage cultivation media in concrete ponds. This was shown from the results of the SPSS model output with multiple linear correlation analysis with a significance value of OCR at 0.538 and SOD at 0.112. Stocking density treatment was more influential as a cause of stress in grouper fish at a significance value <0,05.
Physiological and Hematological Responses in Cantang Grouper (Epinephelus fuscoguttatus x Epinephelus lanceolatus) Against the Salinity of Different Media Larasati, Anastasya Dewi; Widodo, Langgeng; Melinda Kusuma Ningrum; Ridwansyah; Karunia, Fitria; Sulmartiwi, Laksmi; Mahasri, Gunanti; Triastuti, Rr Juni; Lutfiyah, Lailatul
Journal of Aquaculture and Fish Health Vol. 14 No. 3 (2025): JAFH Vol. 14 No. 3 September 2025
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v14i3.71620

Abstract

Fish Cantang grouper is a cross between a female tiger grouper (Ephinepelus fuscoguttatus) and a male kertang grouper (Ephinepelus lanceolatus), has a high economic value. Salinity is a water quality factor that affects cultivation because salinity has osmotic pressure that can cause changes in physiological activity and stress in fish if it is not in accordance with the habitat. Stress in fish causes the release of cortisol and catecholamine hormones. The catecholamine hormone then increases the glycogenolysis process, regulates cardiovascular and respiratory function, in addition to increasing cortisol and blood glucose. Stress in fish is also indicated by a hematological response in the form of changes in the number of erythrocytes. This study aims to determine the effect of different salinity treatments on physiological and hematological responses. The results of the analysis of variance (ANOVA) carried out from the initial observation to 24 hours showed that each salinity treatment had a significantly different effect on blood cortisol, blood glucose, and erythrocyte levels, with a value (p <0.05). The highest average blood cortisol was in the 36 ppt treatment at 24-hour observation time. The average blood glucose increased from before being treated, until receiving treatment for up to 24 hours. The highest average was obtained at a salinity of 36 ppt, with observation for 24 hours. The results obtained from the study showed that the highest number of erythrocyte cells at 24 hours was in the P2 treatment (36 ppt), namely 1.15 x 106 cells/mm³.
Cyprinus carpio hematological changes after dietary Coffea canephora leaf extract for Aeromonas hydrophila infection Sulistyoningrum, Shandy; Mahasri, Gunanti; Ulkhaq, Mohammad Faizal; Kenconojati, Hapsari
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 12, No 2 (2023): AUGUST 2023
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.2.28682

Abstract

Coffea canephora or robusta coffee was known as a medicinal plant that admitted various biological properties. However, its application as a medicinal plant in fish disease treatment has not been reported. This study aimed to investigate the effect of dietary robusta coffee leaf (RCL) extract on the survival rate and hematology of Cyprinus carpio infected by Aeromonas hydrophila. Two hundred fish were randomly divided into five treatment groups consisting of uninfected fish (UF), infected fish (IF), and a diet supplemented with different concentrations of RCL extract (30%, 40%, and 50%). The negative control group was injected by NaCl 0.9% and other treatment groups were injected by A. hydrophila. Feeding treatment was carried out for 7 days after clinical symptoms appeared. The results showed that RCL extract increases the survival rate and hematological parameters (number of erythrocytes, hemoglobin, and lymphocytes) significantly (P0.05). The highest survival rate (100%) was recorded in groups that accepted 50% of RCL extract. This current study indicates that RCL extract could be considered a potent medicinal plant for treating fish disease caused by A. hydrophila at the optimum dose of 50%.Keywords:AeromonasCoffea canephoraExtractHematologyCyprinus carpio
Co-Authors A. Shofy Mubarak Abdul Manan Abdul Manan Abyan Farras Adamu Ayubu Mwendolwa Adamu Ayubu Mwendolwa Adiacahya, Eren Aditya Gita Rohmatullah Adriana Monica Sahidu Agung Pamuji Rahayu Agus Nazarudin Yahya Ahmad Shofy Mubarak Akhmad Taufiq Mukti Alanosi Noor Muhammad Alfan Prianggara Alfin Tauhid Alim Isnansetyo Almira Fardani Lahay Alvira Febrianti Pratiwi Anam, M Khairul Anggun Khoirun Nikmah Anggun Nurani Citrowati Anisa, Hosia Anord Charles Nkuba Apri Supii Ardilas Heryamin Arika Juniarsih Arya Witantama Bahtiar, Sadida Anindya Berliana A Bernathdo Mahendra Robbi Putra Bidayatul Afifah Boedi S. Rahardja Boedi Setya Rahardja Browijoyo Santanamurti Cintia Larasati DARMAWAN SETIA BUDI Desak Ketut Sekar Cempaka Putri Dewi, Nina Nurmalia Dheani Nur Ahadya Syifa Chaerunissa Dieswinta Hardika Aris dika dika Dita Wisudyawati Eka Saputra Elangga Sony Widiharsono Elisabeth Benita Indarmastuti Endah Sih Prihatini Endang Dewi Masithah Era Insivitawati Ewang Mahendra Putra Fahdi Putra Utama Faisol Mas&#039;ud Faisol Mas’ud Farid Nur Salim Farizka Vinka Trinendyah Ferry Dwi Firmansyah Liananda Firly Waliani Rahma Fuad Fuquh Rahmat Shaleh Gian Suryanatha Hartawan Hari Suprapto Heru Suryanto Ika Purnamasari Ika Putri, Lia Oktavia Ikmalia A Ikmalia Amali Indah Hidayati Imani Iqbal Ghazali Irvansyah Irvansyah Isroni, Wahyu Kadek Racmawati Karunia, Fitria Kenconojati, Hapsari Kismiyati , Koesnoto Koesnoto Kusnoto Kusnoto Lailatul Lutfiyah, Lailatul Laksmi Sulmartiwi Laksmi Surmartiwi Larasati, Anastasya Dewi Lestari Wilujeng Lia Oktavia Ika Putri Lia Oktavia Ika Putri Lilis Cahaya Septiana Linnya Prima Agustin Lucia Tri Suwanti, Lucia Tri Luthfiana Aprilianita Sari Lyla Wulandari M Ervany Eshmat M Iqbal Satria Mandele, M. Muhtar Mayangsari, Cholivia Melinda Kusuma Ningrum Miftakhul Munir Moch Saad Mochammad Dwi Hardhianto Mohamad Yusuf Mohammad Faizal Ulkhaq Mufasirin Muhamad Amin Muhamad Amin Muhammad Amin Muhammad Arief Muhammad Herman Muhammad Hilmy Maulana Muhammad Musa N. Juni Triastuti Nafis Putra Laksana Cholil Nanuk Qomariyah Nedi Nedi Netty Sreani Nico Rahman Caesar Niluh Suwasanti Norma Isnawati Nugroho, Sefilia Adhi Nunuk Dyah Retno Lastuti Nur Fais Nurlita Abdulgani Nurul Kumalasari Ochtavia, Sherly Permata Jelita, Chelsea Prayogo Prayogo Pristita Widyastuti Puguh Yugo Wijanarko Putra, Bernathdo Mahendra Robbi Putri, Desak Ketut Sekar Cempaka Rachma Woro Anggarani Racmawati, Kadek Rahayu Kusdarwati Rahayu Kusdarwati Ridwansyah Ririn Agustiya Riris Ulfiana Riza Aryani Romziah Sidik Rozi Rozi Rr. Juni Triastuti Salman Aldo Alfaresi Samara, Syifania Hanifah Santanumurti, Muhammad Browijoyo Sapto Andriyono Saputra, Alfian Rahmadhani Satria Hani Sari, Putri Desi Wulan Saroso, Heri Setiawan Koesdarto Shandy Sulistyoningrum Sherly Ochtavia Shohifah, Isnatul Umu Siti Hamidah Sri Mulyati Sri Mulyati Sri Subekti Sri Subekti Sri Subekti Sudarno Sudarno Sudarno, Sudarno Sulistyoningrum, Shandy Suwasanti, Niluh Suzanita Utama Titom Gusmana Putra Perdana Ulia Fajriah Veryl Hasan W. Angan Indrawan Widodo, Langgeng Wijanarko, Puguh Yugo Wiwin Sumiati Wiwin Sumiati Woro Hastuti Satyantini Woro Hastuti Setyantini Wurlina, W Yanuhar, Uun Yudi Cahyoko Yunifar Amad