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KOMBINASI NANOPARTIKEL KITOSAN DAN TURUNAN SELULOSA DALAM PEMBUATAN EDIBLE COATING TERHADAP AKTIVITAS BAKTERI DAN ORGANOLEPTIK PEMPEK IKAN TENGGIRI Fadiah Shabrina; Gunanti Mahasri; A. Shofy Mubarak
Journal of Aquaculture Science Vol 9 No 2 (2024): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/joas.v9i2.62040

Abstract

Pempek is a very popular traditional Indonesian food because it has a delicious taste. However, the shelf life of pempek is only about 16 hours at room temperature. This study aims to develop and evaluate edible coatings based on cellulose and chitosan nanoparticles (CSNPs) to extend the shelf life of pempek from Tenggiri fish (Scomberomorus commerson). This study is experimental research divided into two stages. The first stage involves characterizing the edible coating using a Completely Randomized Design (CRD), with tests including antimicrobial activity, water absorption, and viscosity, conducted with 5 treatments and 4 repetitions. The second stage involves applying the coating to pempek and evaluating its shelf life, focusing on TPC and organoleptic qualities using a two-factor CRD with 3×6 treatments and 3 repetitions. The results showed a significant effect (P<0.05) of various formulations on the characteristics of edible coatings. The results showed that the variation of cellulose derivatives and CSNP in the preparation of edible coating significantly affected the coating characteristics (P<0.05). The combined formulation of CMC and CSNP (P5) was selected as the pempek coating, with antibacterial activity of 9.31 mm, water absorption of 1.23%, and solution viscosity of 33.50 cPa.s. In the second stage, the treatment of pempek coating with CMC and CSNP (C2) effectively maintained the quality of pempek until day 3 at room temperature and day 12 at refrigerator temperature.
Stress Response and Pathology of Hybrid Groupers (Epinephelus lanceolatus × Epinephelus fuscoguttatus) Infested Trichodina sp. Maintained in Hatchery from Bali, Indonesia Lia Oktavia Ika Putri; Gunanti Mahasri; Ahmad Shofy Mubarak; Lilis Cahaya Septiana
Journal of Aquaculture Science Vol 10 No 1 (2025): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/joas.v10i1.67885

Abstract

The demand for grouper fish continues to increase, with the Ministry of Fisheries' annual production target of 9,000 tons. However, intensive cultivation systems pose risks due to poor water quality, making grouper fish susceptible to disease and health problems. This study investigated the impact of Trichodina sp. infection on the blood glucose levels of hybrid grouper fish. The study was conducted in May 2024 by observing 30 fish and measuring blood glucose. The results showed that 20 fish showed glucose levels above the normal range (> 57.6 mg/dL), ranging from 61 to 114 mg/dL. In contrast, nine fish had glucose levels in the normal range (28.8 - 57.5 mg/dL), and one fish had low glucose levels of 26.7 mg/dL. High glucose levels in most fish indicate stress or metabolic disorders, possibly related to Trichodina sp. infestation. This parasitic infection can compromise the fish's immune system, making the fish more susceptible to disease. The results emphasize the need for effective parasite management and optimal water quality in intensive culture systems. To maintain grouper health and productivity, it is important to monitor water quality, control ectoparasites, and conduct further research on the long-term effects of these infections on blood glucose and overall health.
Identification of Vibrio Species and Total Vibrio Count in Kidney Tissue of Humpback Grouper (Cromileptes altivelis) Reared at Different Stocking Densities in Pond-Based Net Cages Nafila Junia Azadia; Gunanti Mahasri; Abdul Manan
Journal of Aquaculture Science Vol 11 No 1 (2026): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/joas.v11i1.78913

Abstract

The humpback grouper (Cromileptes altivelis) is a high-demand grouper species, both domestically and internationally. However, the utilisation of this cultivation system still faces obstacles, especially in the field of disease. This research was conducted to determine the total Vibrio count (TVC) and identify Vibrio species in the kidney tissue of humpback grouper reared with different stocking densities in pond-based net cages. The TVC examination method was carried out using serial dilution and grown on selective TCBS media. Bacterial identification was carried out by observing colony colour, Gram staining, and biochemical tests (catalase, SIM, TSIA, OF, and MRVP test). The results show that the highest TVC value was obtained from high-density treatment (1.9 × 104 CFU/g), meanwhile, the lowest TVC value was obtained from normal-density treatment (0.1 × 101 CFU/g). The results of bacterial identification showed that 3 of the 5 isolates obtained during the study (V1, V2, V4) were similar to V. fluvialis, V. alginolyticus, and V. parahaemolyticus based on biochemical tests. This study concluded that Vibrio bacteria similar to V. fluvialis, V. alginolyticus, and V. parahaemolyticus were found in the kidneys of grouper fish cultured at different stocking densities in net cages, and that the TVC values remained within the normal range.
Co-Authors A. Shofy Mubarak Abdul Manan Abdul Manan Abdul Manan Abyan Farras Adamu Ayubu Mwendolwa Adamu Ayubu Mwendolwa Adelia Inas Shafira Adiacahya, Eren Aditya Gita Rohmatullah Afifah Elok Firdaus Agniny, Siswi Fililmi Agung Pamuji Rahayu Agus Nazarudin Yahya Ahmad Shofy Mubarak Akhmad Taufiq Mukti Alanosi Noor Muhammad Alfan Prianggara Alfin Tauhid Alim Isnansetyo Almira Fardani Lahay Alvira Febrianti Pratiwi Anam, M Khairul Anggun Khoirun Nikmah Anggun Nurani Citrowati Anisa, Hosia Anord Charles Nkuba Apri Supii Ardilas Heryamin Arika Juniarsih Arya Witantama Bahtiar, Sadida Anindya Berliana A Bernathdo Mahendra Robbi Putra Bidayatul Afifah Boedi S. Rahardja Boedi Setya Rahardja Browijoyo Santanamurti Cintia Larasati DARMAWAN SETIA BUDI Daruti Dinda Nindarwi Daud Dharmansyah Desak Ketut Sekar Cempaka Putri Dheani Nur Ahadya Syifa Chaerunissa Dieswinta Hardika Aris dika dika Dita Wisudyawati Eka Saputra Elangga Sony Widiharsono Elisabeth Benita Indarmastuti Endah Sih Prihatini Endah Sih Prihatini Endang Dewi Masithah Era Insivitawati Ewang Mahendra Putra Fadiah Shabrina Fahdi Putra Utama Faisol Mas&#039;ud Faisol Mas’ud Faisol Mas’ud Farid Nur Salim Farizka Vinka Trinendyah Farras Rana Hafizhah Ferry Dwi Firmansyah Liananda Firly Waliani Rahma Fransisca, Fida Fuad Fuquh Rahmat Shaleh Fuquh Rahmat Shaleh Gian Suryanatha Hartawan Hari Suprapto Heru Suryanto Ika Purnamasari Ika Putri, Lia Oktavia Ikmalia A Ikmalia Amali Indah Hidayati Imani Iqbal Ghazali Irvansyah Irvansyah Isroni, Wahyu Kadek Racmawati Karunia, Fitria Kenconojati, Hapsari Kismiyati , Koesnoto Koesnoto Kusnoto Kusnoto Lailatul Lutfiyah, Lailatul Laksmi Sulmartiwi Laksmi Surmartiwi Larasati, Anastasya Dewi Lestari Wilujeng Lia Oktavia Ika Putri Lia Oktavia Ika Putri Lia Oktavia Ika Putri Lilis Cahaya Septiana Lilis Cahaya Septiana Linnya Prima Agustin Lucia Tri Suwanti, Lucia Tri Luthfiana Aprilianita Sari Lyla Wulandari M Ervany Eshmat M Iqbal Satria M. Muhtar Mandele Mayangsari, Cholivia Melinda Kusuma Ningrum Miftakhul Munir Moch Saad Mochammad Dwi Hardhianto Mohamad Yusuf Mohammad Faizal Ulkhaq Mufasirin Muhamad Amin Muhamad Amin Muhammad Amin Muhammad Arief Muhammad Herman Muhammad Hilmy Maulana Muhammad Musa N. Juni Triastuti Nafila Junia Azadia Nafis Putra Laksana Cholil Nanik Siti Aminah Nanuk Qomariyah Nedi Nedi Netty Sreani Nico Rahman Caesar Niluh Suwasanti Nina Nurmalia Dewi Norma Isnawati Nugroho, Sefilia Adhi Nunuk Dyah Retno Lastuti Nur Fais Nurlita Abdulgani Nurul Kumalasari Ochtavia, Sherly Permata Jelita, Chelsea Prayogo Prayogo Pristita Widyastuti Puguh Yugo Wijanarko Putra, Bernathdo Mahendra Robbi Putri Garnet Endo Mahata Putri, Desak Ketut Sekar Cempaka Rachma Woro Anggarani Racmawati, Kadek Rahayu Kusdarwati Rahayu Kusdarwati Ridwansyah Ririn Agustiya Riris Ulfiana Riza Aryani Romziah Sidik Rozi Rozi Rr. Juni Triastuti Sahidu, Adriana Monica Salman Aldo Alfaresi Samara, Syifania Hanifah Santanumurti, Muhammad Browijoyo Sapto Andriyono Saputra, Alfian Rahmadhani Satria Hani Sari, Putri Desi Wulan Saroso, Heri Setiawan Koesdarto Setyantini, Woro Hastuti Shandy Sulistyoningrum Sherly Ochtavia Shohifah, Isnatul Umu Siti Hamidah Sri Mulyati Sri Mulyati Sri Subekti Sri Subekti Sri Subekti Sudarno Sudarno Sudarno, Sudarno Sulistyoningrum, Shandy Suwasanti, Niluh Suzanita Utama Titom Gusmana Putra Perdana Ulia Fajriah Veryl Hasan W. Angan Indrawan Widodo, Langgeng Wijanarko, Puguh Yugo Wiwin Sumiati Wiwin Sumiati Woro Hastuti Satyantini Wurlina, W Yanuhar, Uun Yudi Cahyoko Yunifar Amad Yusma Latifatul Ummah