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All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Agrotrop : Journal on Agriculture Science METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Itepa : Jurnal Ilmu dan Teknologi Pangan Jurnal Veteriner Jurnal Biologi Udayana Bumi Lestari Ecotrophic, Journal of Environmental Science Jurnal Bios Logos Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology BIOTROPIA - The Southeast Asian Journal of Tropical Biology Agrointek Biotropika Jurnal Biosains Pascasarjana Jurnal Biologi Universitas Andalas Biota ANDHARUPA REINWARDTIA Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Floribunda Jurnal Aplikasi dan Inovasi Iptek (JASINTEK) Indigenous Biologi BIO-CONS: Jurnal Biologi dan Konservasi Journal of Natural Sciences BIO-CONS: Jurnal Biologi dan Konservasi Reinwardtia SIMBIOSIS
MADE PHARMAWATI
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Veterinary Other

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VARIASI GENETIK MASYARAKAT SOROH PANDE DI KABUPATEN GIANYAR BERDASARKAN TIGA LOKUS DNA MIKROSATELIT AUTOSOM Gusti Ayu Putri Arnila; I Ketut Junitha; Made Pharmawati
Jurnal Biologi Udayana Vol 20 No 1 (2016): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Perkembangan ilmu forensik berkaitan erat dengan penggunaan analisa DNA mikrosatelit sebagai salah satu sarana yang paling akurat untuk mengungkap identitas seseorang. Penelitian ini dilaksanakan untuk mengetahuiragam alel yang tersebar pada masyarakat soroh Pande di Kabupaten Gianyar dengan menggunakan tiga lokus DNA mikrosatelit autosom yaitu D2S1338, D13S317 dan D16S539.  Berdasarkan hasil analisa ditemukan 23 ragam alel dengan rata-rata 7,3 per lokus. Rata-rata nilai heterozigositas dari tiga lokus yang digunakan adalah 0,817 ± 0,029 menunjukkan bahwa secara genetik masyarakat soroh Pande sangat bervariasi. Rata-rata nilai Power of Discrimination adalah 0,893. Tingginya nilai Power of Discrimination menunjukkan bahwa ketiga lokus ini baik digunakan untuk membedakan antar individu pada kepentingan forensik.
Keragaman genetik masyarakat Pande Bangke Mawong di Desa Sumita, Kabupaten Gianyar, Bali berdasarkan penanda DNA mikrosatelit kromosom Y I Gde Suryadi; I Ketut Junitha; Made Pharmawati
Jurnal Biologi Udayana Vol 24 No 1 (2020): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (402.271 KB) | DOI: 10.24843/JBIOUNUD.2020.v24.i01.p03

Abstract

Masyarakat Pande Bangke Mawong di Desa Sumita merupakan salah satu bagian klan besar Pande di Bali. Asal-usul masyarakat Pande di Desa Sumita tersebut diketahui masih menjadi kontroversi. Penelitian ini dilakukan untuk mengetahui keragaman genetik masyarakat Pande Bangke Mawong di Desa Sumita berdasarkan penanda DNA mikrosatelit kromosom Y. Sampel diambil di Desa Sumita dan beberapa desa di Bali sebagai pembanding dengan metode purposive sampling. Tahapan penelitian meliputi ekstraksi DNA dengan metode fenol-kloroform yang telah dimodifikasi, amplifikasi DNA menggunakan penanda DNA mikrosatelit dengan lima macam primer yaitu DYS19, DYS390, DYS393, DYS395, dan SRY, elektroforesis produk PCR dengan Polyacrilamide Gel Electrophoresis (PAGE) 10%, visualisasi DNA dengan metode pewarnaan perak nitrat, dan DNA typing ditentukan melalui ukuran panjang DNA amplikon dengan mengukur jarak migrasinya pada gel yang diplot pada kertas semilog. Data frekuensi alel dan keragaman genetik dianalisis menggunakan software GenAlex 6.5. Hubungan kekerabatan dianalisis dengan metode UPGMA menggunakan software MEGA 7. Ditemukan delapan alel dan tiga haplotipe pada masyarakat Pande Bangke Mawong di Desa Sumita. Pada masyarakat Pande lainnya ditemukan 12 alel dan enam haplotipe. Nilai keragaman genetik masyarakat Pande di Desa Sumita adalah 0,035±0,014, sedangkan pada masyarakat Pande lainnya sebesar 0,432±0,112. Ragam alel dan haplotipe yang ditemukan pada masyarakat Pande Bangke Mawong menunjukkan adanya proses mikroevolusi yang disebabkan mutasi genetik. Hubungan kekerabatan masyarakat Pande di Desa Sumita paling dekat dengan masyarakat Pande di Desa Tenganan Pegringsingan dan Desa Renon yang dikenal sebagai salah satu desa tua di Bali.
IDENTIFIKASI MAKROZOOBENTHOS DI TUKAD BAUSAN, DESA PERERENAN, KABUPATEN BADUNG, BALI Ni Made Suartin; Ni Wayan Sudatri; Made Pharmawati; A. A. G. Raka Dalem
ECOTROPHIC : Jurnal Ilmu Lingkungan (Journal of Environmental Science) Vol 5 No 1
Publisher : Master Program of Environmental Science, Postgraduate Program of Udayana University

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Abstract

A macrozoobenthos study was conducted at Bausan River, Pererenan village, Badung regency, Bali between September and December, 2006. There were six sampling stations were determined purposively. At each station, five unit of square plots of 40 cm x 40 (in maximum solum depth) samples were taken. The result showed that there were seventeen species of macrozoobenthos were found. There was no protected species found in this study. The macrozoobenthos had 2,28 level of index diversity. This indicated that the ecosystem at this area was in a stable condition with a medium level of macrozoobenthos diversity.
SUCCESSFUL GENETIC CHARACTERIZATION OF BULLET TUNA (Auxis rochei) USING MICROSATELLITE MARKERS IN KARANGASEM BALI Maya Agustina; Made Pharmawati; Ketut Junitha
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 17, No 3 (2021): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.17.3.%p

Abstract

Bullet Tuna (Auxis rochei) is classified under the neritic tuna group, which plays an essential role in small-scale fisheries in Karangasem Bali. The increasing catch of bullet tuna indicated its stock probably under threat. Therefore, genetic characterization is often required as the first step before building a conservation program. This study aims to categorize bullet tuna DNA using microsatellite. Of all five loci used, all were high polymorphism-type, with the number of alleles per locus varied between 18-27. Successful PCR (Polymerase Chain Reaction) created relatively high DNA concentration, ranging from 27.050 to 237.05 ng/ul, with a DNA purity level ranging from 2.073 to 2.239. Overall, the stock condition allegedly still in good condition, marked with high genes diversity (Ho=0.367-0.767 and He=0.934-0.966). All loci used can be amplified and well described.
Genetic Diversity and Population Structure of Bullet Tuna (Auxis rochei) from Bali and Its Adjacent Waters Maya Agustina; Bram Setyadji; Made Pharmawati; I ketut Junitha
HAYATI Journal of Biosciences Vol. 29 No. 4 (2022): July 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.4.507-514

Abstract

Bullet tuna (Auxis rochei) dominates the neritic tuna catch, especially from the purse seine fleet within the western and southern Indonesian waters. However, high catches can lead to stock depletion and lower genetic diversity due to possible inbreeding. Therefore, population genetic information is important in monitoring the sustainability of fish stocks and proposing an appropriate species-specific conservation strategy. This study aimed to analyze the genetic diversity, population structure, and kinship relationship of bullet tuna in Bali and its adjacent waters. Sampling was carried out in September 2020 at landing sites/ports representing the north, east, south, and west region, whereas at least 30 samples were acquired at each location. The result showed that the DNA concentration obtained could produce DNA bands with allele length ranged from 94-260 bp. Observed heterozygosity (Ho) was around 0.440-0.627. While the expected heterozygosity (He) was between 0.932-0.945. The genetic variation among population, within-population, and individuals was 0.36%, 41.04%, and 58.60%, respectively. The results of the analysis of genetic diversity between individuals in the population showed very high genetic diversity. The population structure of the bullet tuna landed in West Bali, East Bali, South Bali and North Bali is the same population stock. The kinship relationship indicates that the four populations are closely related genetically.
Pengaruh Ethyl Methane Sulphonate (EMS) Terhadap Pertumbuhan dan Variasi Tanaman Marigold (Tagetes sp.) NI MADE DIAN PRATIWI; MADE PHARMAWATI; IDA AYU ASTARINI
Agrotrop : Journal on Agriculture Science Vol 3 No 1 (2013)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

The Effect of Ethyl Methane Sulphonate (EMS) on Growth and Variations of Marigold (Tagetes sp.) The aims of this research are to determine the variation of marigold (Tagetes sp) derived from seed treated with EMS and to recommend the EMS concentrations that are able to induce varietion. Seeds of marigold cv Narai Orange were soaked in water for 6 hours, followed by soaking in EMS at concentration of 0%, 0.3%, 0.6% and 0.9% for 4 hours. This study employed Randomized Complete Blok Design with 10 replicates and each replicate consisted of 10 plants. Six plants were randomly chosen for measurements. The total number of samples observed were 240 plants. Observations were made on the percentage of the growth, plant height, number of leaves, number of branches, diameter and weight of flowers. Data obtained from the observations were analyzed using Analysis of Variance (ANOVA), followed by DMRT (Duncan’s Multiple Range Test) if there is a significant difference between treatments. The EMS treatment reduced all characters observed. The EMS concentration of 0.6% showed plant that had yellow flowers. The 0.9% EMS treatment resulted in one plant with chimera, 6 dwarf plants, 2 plants with thin stems, and 1 short plant with many branches. Untreated plants did not show any variation.
Induksi Mutasi Tanaman Cabai Merah (Capsicum Annuum L.) dengan Ethyl Methanesulfonate pada Berbagai Tingkat Waktu Perendaman I MADE AGUS WIARTANA; MADE PHARMAWATI; I KETUT SUADA
Agrotrop : Journal on Agriculture Science Vol 4 No 1 (2014)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

Induction of Mutation of Red Chili (Capsicum Annuum L.) Using Ethyl Methanesulfonate at Several Soaking Periods.One way to increase genetic variation is through induced mutation usingchemical mutagen. Ethyl methanesulfonate (EMS) is a chemical compound that can cause mutationsand commonly used in plant. In this study seeds of red chili were treated using EMS 1% through seedsoaking. Seeds of red chili were soaked with EMS 1% in phosphate buffer pH 7 for 6, 9, 12 and 15hours at room temperature. As control, seeds were soaked in phosphate buffer pH 7. This study aimsto evaluate, physiological and reproductive characters of plants after treated with EMS. Experimentwas conducted in an open field with 5 replicates for each treatment. Results showed that concentrationof chlorophyll a, b and total chlorophyll increased in plants derived from seed treated with EMS 1% for9 hours compared to control and other soaking periods. Soaking seeds with EMS 1% for 12 hoursincreased viability of pollen compared to control and other treatments. The first time of floweringoccurred earlier at 6 and 9 hours soaking period.
MOLECULAR PHYLOGENY OF MAIDENHAIR FERN GENUS ADIANTUM (PTERIDACEAE) FROM LESSER SUNDA ISLANDS INDONESIA BASED ON RBCL AND TRNL-F Wenni Setyo Lestari; Bayu Adjie; Tassanai Jaruwatanaphan; Yasuyuki Watano; Made Pharmawati
REINWARDTIA Vol 14, No 1 (2014): Vol. 14 No. 1
Publisher : Research Center for Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/reinwardtia.v14i1.409

Abstract

The Lesser Sunda Islands of Indonesia are composed of small islands scattered from Bali to Timor Island. We analyzed a molecular phylogeny of Adiantum collected from Lesser Sunda Islands to reveal its phylogenetic relationships. A total of 12 species of Adiantum from this region and seven species from Java Island were collected and used in this study. Two cpDNA regions (rbcL and trnL-F) were chosen as markers and phylogenetic analyses were conducted using Neighbour-Joining (NJ) and Maximum Parsimony (MP) methods. The tree topologies reconstructed by NJ and MP from specimens used in this study and other species downloaded from GenBank are congruent in which trees are divided into five major clades. Adiantum species of Lesser Sunda Islands are not monophyletic and comprises three clades, i. e. Clade I composed of A. hispidulum group, Clade III composed of A. peruvianum group and Clade IV or A. caudatum group, each together with extra-Lesser Sunda samples. No sample from Lesser Sunda Islands examined is located in Clade II (A. tenerum group) and V (A. capillus-veneris group).
PEMBENTUKAN KALUS, TUNAS DAN AKAR PADA KULTUR ANGGUR BALI (Vitis vinifera cv Alphonse Lavallee) DENGAN PEMBERIAN NAA dan BAP Made Pharmawati; Made Ria Defiani
BIO-CONS : Jurnal Biologi dan Konservasi Vol 3 No 1 (2021): BIO-CONS: Jurnal Biologi dan Konservasi
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31537/biocons.v3i1.432

Abstract

Anggur Bali (Vitis vinifera L. cv Alphonse Lavallee) merupakan salah satu tanaman buah yang banyak dibudidayakan di Kabupaten Buleleng, Bali. Kebutuhan akan bibit anggur Bali cukup tinggi, sehingga diperlukan teknik perbanyakan yang dapat menghasilkan bibit dalam jumlah besar. Salah satu teknik yang dapat digunakan adalah kultur jaringan tanaman. Penelitian ini bertujuan menganalisis respon eksplan cabang yang masih muda terhadap zat pengatur tumbuh (ZPT) NAA dan BAP pada kultur Anggur Bali. Cabang tanaman anggur yang masih muda diambil dari salah satu perkebunan Anggur Bali di Kecamatan Seririt, Kabupaten Buleleng, Bali. Dilakukan sterilisasi eksplan dan ditanam pada medium MS dengan penambahan zat pengatur tumbuh NAA dan BAP dalam beberapa kombinasi. Pengamatan dilakukan terhadap pembentukan kalus, tunas dan akar. Hasil menunjukkan bahwa pembentukan kalus, tunas dan akar Anggur Bali dengan teknik kultur jaringan dipengaruhi oleh zat pengatur tumbuh NAA dan BAP. Persentase tertinggi kultur yang membentuk kalus terjadi pada penambahan 1,5 mg/L NAA dan 2 mg/L BAP, tetapi skor kalus yang terbentuk tertinggi pada kombinasi 1 mg/L NAA dengan 4 mg/L BAP dan kombinasi 1,5 mg/L NAA dengan 4 mg/L BAP. Tunas terbentuk pada perlakuan 4 mg/L BAP tanpa NAA dan perlakuan 1 mg/L NAA dengan 4 mg/l BAP. Persentase kultur yang membentuk akar dan jumlah akar terbanyak terjadi pada pemberian kombinasi 1 mg/L NAA dengan 2 mg/L BAP. Perubahan kecil pada konsentrasi ZPT mengakibatkan perubahan respon tanaman, sehingga penelitian lebih lanjut diperlukan untuk mendapatkan kombinasi dan konsentrasi ZPT yang tepat untuk menghasilkan plantlet dalam jumlah besar.
Penggandaan Kromosom Marigold (Tagetes erecta L.) dengan Perlakuan Kolkisin Ida Ayu Ratih Purnama Dewi; Made Pharmawati
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 35, No 3 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2018.35.3.773

Abstract

Penelitian ini bertujuan untuk menganalisis pengaruh pemberian kolkisin terhadap jumlah kromosom marigold serta dapat mengetahui konsentrasi kolkisin yang efektif untuk menginduksi poliploidi pada kecambah marigold.  Kecambah diberi direndam dalam kolkisin dengan konsentrasi 0,1% dan 0,2% selama 6 jam Sebagai kontrol digunakan akuades. Parameter yang diamati adalah jumlah kromosom ujung akar marigold pada masing-masing perlakuan. Pengamatan kromosom dilakukan dengan metode squash ujung akar. Pemberian kolkisin  menyebabkan penggandaan jumlah kromosom kecambah marigold. Rata-rata jumlah kromosom kecambah marigold pada kontrol adalah 2n=2x=20,5, sementara  rata-rata jumlah kromosom yang diberi perlakuan kolkisin 0,1% dan 0,2 % berturut-turut yaitu 2n=4x=42 dan 2n=4x=43.
Co-Authors A. A. Putri Ardyanti A.A.S. A. Sukmaningsih Agriani Dewinta Anak Agung Ayu Putri Ardyanti Anak Agung Gde Raka Dalem Andi Bahtiar Batti Andrianus Sembiring, Andrianus Aziz, Alifya Ibnu Bayu Adjie Bayu Adjie Bayu Adjie Bram Setyadji Deny Suhermawan Yusup Dian Catur Prayantini Dwi Nur Rikhma Sari Eka Fibayani Imaniar Eka Fibayani Imaniar Ema Hendriyani Faukha Aisyatur Rahma Fransiska Nitti Fransiskus Jimmy Roga Gde Evayanti, Luh GUIJUN YAN Gusti Ayu Putri Arnila I Gde Suryadi I Gede Putu Ardhana I Gusti Agung Eka Saraswati I Gusti Ayu Agung Pradnya Paramitha I Gusti Ngurah Kade Mahardika I Ketut Catur Wiguna I Ketut Junitha I Ketut Suada I Komang Adi Widyastama I MADE AGUS WIARTANA I Made Anom Sutrisna Wijaya I Made Murna I Putu Agus Tirta Cahyana I Putu Satwika I Wayan Arnata I Wayan Rosiana I. Setyawati I.G.A.S. Wahyuni I.M.A.S. Wijaya IAN JAMES MACFARLANE IDA AYU ASTARINI Ida Ayu Ratih Purnama Dewi Ida Bagus Made Suaskara Imaniar, Eka Fibayani Indah Tria Hoky Inna Narayani IRIANI SETYAWATI Joko Raharjo, Sentot Junita Hardini Ketut Queena Fredlina Luh Putu Wrasiati Made Ria Defiani Maharani, Rai Janaki Maliza Kurnia Maya Agustina Maya Agustina Mery Wintari Ngurah Intan Wiratmini Ni Kadek Dewi Rustini NI KADEK YUNITA SARI Ni LUH ARPIWI Ni Luh Ayu Jami Wistiani, Ni Luh Ayu Jami Ni Luh Putu Agustini Ni Luh Watiniasih Ni Luh Wayan Yulia Mirayanti NI MADE DIAN PRATIWI Ni Made Gari Ni Made Sastriyani Wiendra Ni Made Sastriyani Wiendra Ni Made Suartin Ni Nyoman Wirasiti Ni Putu Adriani Astiti Ni Putu Eka Krisnayanti Ni Wayan Ayu Wiartini Ni Wayan Karolina Ni Wayan Sudatri PATRICK FINNEGAN Putera, I Kadek Aridena Putu Angga Wiradana Putu Apriliani Putu Yunita Putri Aryani Retno Kawuri Rifky Firmansyah Anwar Satwika, I Putu Septarini Dian Anitasari Septarini Dian Anitasari Siddique, Kadambot.H.M. Tassanai Jaruwatanaphan Tassanai Jaruwatanaphan Tassanai Jaruwatanaphan, Tassanai Tiwi Wati Uslan UUL SHOVI NURKAMILA Vandi Dama Setiawan Vanesa Martida Vanesa Martida Viryanando Evan Rahardja WAHYU WIDOWATI Wawan Sujarwo WENNI SETYO LESTARI Wenni Setyo Lestari Wenni Setyo Lestari, Wenni Setyo Wiguna, I Ketut Catur Wijaya, Putu Austin Widyasari Wildan Mujahidul Basyar Yasuyuki Watano Yasuyuki Watano Yasuyuki Watano, Yasuyuki Yenni Ciawi Zaetuna, Hanifa Laila