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Zahara Zahara
Politeknik Negeri Padang
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Journal : Eruditio : Indonesia Journal of Food and Drug Safety

 1 
Validasi Metode Analisis Penetapan Kadar 4-Amino-3-Nitrophenol Dalam Pewarna Rambut Oksidator Prabaning Budi, Hasti Kusuma; Zahara, Zahara
Eruditio : Indonesia Journal of Food and Drug Safety Vol 4 No 1 (2023): Edisi Desember
Publisher : Badan Pengawas Obat dan Makanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54384/eruditio.v4i1.164

Abstract

4-amino-3-nitrophenol (C6H6N2O3) has been widely used as an oxidative hair colourant. This compound was a structural isomer of 2-amino-4-nitrophenol (2,4-ANP) and 2-amino-5-nitrophenol (2,5-ANP). All of these compounds have different regulations in Indonesia. The maximum concentration of 4-amino-3-nitrophenol in oxidative hair colourants was 1.5% after being mixed with oxidator agents. Otherwise, 2-amino-4-nitrophenol and 2-amino-5-nitrophenol were prohibited substances contained in cosmetic products. Therefore, it was essential to validate the method to differentiate all these three compounds with a resolution of more than 1.5. The High-Performance Liquid Chromatography with Photo Diode Array Detector using octadecylsilane (C18) column (250 mm x 4.6 mm and 5 μm particle size) has been developed and validated. Acetonitrile HPLC grade and 0.05 M acetic buffer with pH 5.9 (20:80) have been used as mobile phase isocratically. The flow rate and column temperature were 1.0 ml/minute and 40oC, respectively. The validation result showed good linearity, with the correlation coefficient of the method being 1.0. The method is also selective in differentiating all three compounds and is specific to the methylparaben compound. The precision data on the concentration of 3.68 μg/ml, 14.70 μg/ml and 18.38 μg/ml was 0.59%, 1.92% and 0.68% respectively.  The percent recovery of this method on the same concentration above was 100.17-100.91, 99.06-100.39 and 100.38-101.05, respectively. The limit quantitation of this method was 0,07%. All validation parameters of the method have met the requirement so that the analysis method for determination of 4,3-ANP has been proven to be selective, accurate and reliable.
Analisis Zink Pirition dalam Sampo Anti Ketombe secara Kromatografi Cair Kinerja Tinggi untuk Mendukung Pengawasan Pre-Market dan Post-Market Zahara, Zahara; Kurniawati, Farida; Lusianti, Erita
Eruditio : Indonesia Journal of Food and Drug Safety Vol 4 No 2 (2024): June Edition
Publisher : Badan Pengawas Obat dan Makanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54384/eruditio.v4i2.175

Abstract

ZnPtO is widely used as an active substance in anti-dandruff shampoo. Instead of describing its benefits, many articles have reported that ZnPtO can harm the environment and human health. Through the Food and Drugs Administration, Indonesia has been regulated to have a maximum limit of 2.0% for rinse-off hair products. The research aims to develop an accurate and reliable method to determine product ZnPtO level. ZnPtO was analyzed using High-Performance Liquid Chromatography (HPLC) with Photo Diode Array (PDA) at a wavelength of 257.9 nm. This research used a C18 column with dimensions of 250 x 4.6 nm and five μm in particle size. The mobile phase consisted of acetonitrile and a mixture of potassium dihydrogen phosphate solution and disodium EDTA at pH 4.0 (30:70). Column temperature was maintained at 40oC at a 1.0 ml/min flow rate.  The results showed that ZnPtO was detected at a retention time of 7 minutes. The method's correlation coefficient and residual deviation were 0.999% and 0.65%, respectively. Method precision at 20, 100, and 160 μg/ml was 0.6694, 0.4511, and 0.4728%, respectively. Method accuracy at those levels was 98.3 to 100.9%. All validation parameters have fulfilled the qualification. ZnPtO levels contained in anti-dandruff shampoos were 0.0081%, 0.0040%, and 0.016%, respectively. The developed method has proven selective, accurate, and reliable. It can control the quality and safety of anti-dandruff shampoo due to pre-market and post-market surveillance.
Co-Authors -, Anisha Mutia Abdurahman Abdurrahman Abdurrahman*, Abdurrahman Afridian Wirahadi Ahmad Agustiani, Mala agustina, yenni Aidil Akbar Alang Sari, Putrie Aldrin, Ilham Aulia Ali, Muhammad Fauzi Amy Fontanella Ananto, Rangga Putra Andini, Puti Cahyo Anisah Annisa Annisa Anshar, Alya Ledias Ardiansyah, Rahmad Arifqi, Muhammad Ariq, Ziyad Al Arkani, Hari Armel Yentifa Athreebil, Athreebil Azizah, Juwita Azzahra, Audree Bakti Setiawan, Dian Basir, M C. Kifli, Gontom Cahyani, Mhonyca Candra candra Candra, Dori Gusti Alex Chairani, Mira Cut Azizah Cut Santika D. Suretno, Nandari Dandi Aprila Darni, Surya Dedy Djefris Diah Gandoriah Diah Gandoriah Diptaningsari, Danarsi Dita Maretha Rissi Dwi Priyanto Eka Rosalina Eka Siskawati Eka Siskawati Eka Siskawati erdiansyah erdiansyah, erdiansyah Fadillah Sabri Fatimah Zuhra Firman Surya Fitra Oliyan Fitri, Devianty Gusman, Mahesa Firdaus Gustia Westi Hakim, Fauzan halifah, elka Haniva Dela GUSTA Hanum, Fauziah Hasanah, Anggun Desmita Helni Rahma Fitri Henni Fitriani Ifwadi, Ifwadi Ifwandi Ifwandi, Ifwandi Jauza, Alfiyah Zahra Josephine Sudiman Junita Yosephine Sinurat Kiftia, Mariatul Kraugusteeliana Kraugusteeliana Kristina, Desi Kumalasari, Ine Kurniawati, Farida Leoni, Prima Widitia Limbong, Christine Herawati Lukman, Isna Rezkia Lusianti, Erita Lusono, Akhir Magdariza, Magdariza Mai Simahatie MARIA BINTANG Marlina Marlina Masyitah, Istiqamatul Maulina, Vira Mawardi, Rahadian Mediyanti, Risma Meidaliyantisyah, Meidaliyantisyah Mirwati, Yulia Mochammad Imron Awalludin Muhammad Aziz Muharramsyah, Rambang Muliani, Vonny Munawarah Munawarah, Munawarah Murni, Intan Permata Mustika, Rasyidah Mutia, Vina Nahlidar, Nahlidar Najmi Najmi, Najmi Novitasari, Erliana Nur Aini Nur Rohman Nurkhaliza, Lulub Oca Amartya Awdes Oktarina, Neneng Oladokun Nafiu Olaniyi Pingki, Anadiya Prabaning Budi, Hasti Kusuma Prisdinawati, Dwita Purba, Nadinda Oxa Zofandri Putri, Sanniyah Alya Putri, Siti Al Roza Selia Rahmawati, Aninda Salma Rahmayani, Rezkia Rahmi Novalita Rahmi Rahmi Rahmi Rahmi Rahmi, Ayu Raissa, Siti Rakhman, Muhammad Arief Ratna Sari Ratna Unaida, Ratna Reka Seprina, Reka Reno Fithri Meuthia Resti, Nindi Sulvina Mai Retna Qomariah Riski, Arpin Riyanti, Putri Robet Asnawi Sabrina, Najiha Salsabila, Nur Sarumaha, Anastasia Yaniarwana Satria, Teuku Dwirza Saumi, Fazrina Sembiring, Ibrahim Setiawaty, Sri Shafira Hijriya Shalu Kumari Shaofa, Rizki Audiah Silvi Listia Dewi Simahatie, Mai Siti Nurlaili Triwahyuni Slameto Slameto Sufritayati, Sufritayati sukri Sukri, sukri Sumaryanto Sumaryanto, Sumaryanto Sumira, Sumira Surnata, Surnata Sutrisno, Slamet Prasetyo Suwardi Suwardi Syafieh, Syafieh Syafira Ramadhea Jr Syam, Misnar Syamsulrizal Syamsulrizal, Syamsulrizal Syaputra, Hafiz Tarisma, Tantri Tasman Tasman Ulfi Maryati Vebrita, Gina Vivianda, Tricia Wardani, Asri Wardani, Suci Wiwik Andriani Wylis A, Ratna Yanuarti, Mira Yaumidin, Umi Karomah Yeni Marlina Yesmira Syamra, Yesmira YULIA, Meri Zalida Afni Zuraihan Zuraihan