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All Journal Jurnal Veteriner Jurnal Medik Veteriner Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Mitra Mahajana: Jurnal Pengabdian Masyarakat Jurnal Kedokteran Hewan Jurnal Agro Veteriner
Tatik Hernawati
Department Of Veterinary Reproduction, Faculty Of Veterinary Medicine, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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The effect of addition of melon (Cucumis melo L.) flesh juice into Lactated Ringer's-egg yolk extender on spermatozoa plasma membrane integrity and spermatozoa morphological abnormalities of semen of native rooster Pitaloka, Dinda Galuh; Mas'ud Hariadi; Suherni Susilowati; Budi Utomo; Tatik Hernawati; Eka Pramyrtha Hestianah; Samuel Inioluwa Akeju
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 1 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i1.2023.49-58

Abstract

This study aims to determine the effect of adding melon (Cucumis melo L.) juice to Lactated Ringer's-egg yolk extender on the intact plasma membrane (IPM) and morphological abnormalities of native rooster spermatozoa. Five ejaculate each from two native roosters (Gallus gallus domesticus) were divided into four equal volumes. Ejaculate was extended 1:10 (v/v) in Lactated Ringer's-egg yolk without or with the addition of of 2, 4, and 8% (v/v) melon flesh juice, respectively for groups T0, T1, T2, and T3. Extended semen was stored in a refrigerator (5°C) and evaluated every two hours. The results showed that IPM of all groups decreased (p <0.05) consistently, lasting more than 40% for six hours in the T0 and T1 groups and four hours in the T2 and T3 groups. The percentage of spermatozoa morphological abnormalities in all groups stored for six hours was higher (p <0.05) than before storage. However, it was not significantly different (p >0.05) from that which had been stored for two and four hours. It was concluded that native rooster semen extended in Lactated Ringer's-egg yolk without (group T0) or with 2% melon flesh juice (group T1) and stored at 5°C retained spermatozoa plasma membrane integrity of more than 40% and morphological abnormalities of less than 20% for six hours. While the addition of 4 % (T2) and 8% (T3) melon flesh juice maintained the percentage of IPM and spermatozoa morphological abnormalities for only four hours.
Intracervical Artificial Insemination using Frozen Semen of Boer buck on Pote Does at Different Times of Estrus Susilowati, Suherni; Tri Wahyu Suprayogi; Tatik Hernawati; Yossy Imam Candika; Widya Paramita Lokapirnasari
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 1 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i1.2023.18-24

Abstract

Pote goats are kept by the rural community in Bangkalan Regency, Madura Island, Indonesia. Estrus symptoms in goats are usually unclear, causing low fertility. This study aimed to determine the intracervical artificial insemination at early and mid-estrus on the Pote does. The 24 the does were randomly divided into two groups and estrous synchronization with PGF2α of 200 µg intramuscularly, twice injections with 11 days intervals. The T1 groups of estrus the Pote does were inseminated at early estrus (0-12 hours). Meanwhile, T2 groups of estrus the Pote does were inseminated at mid-estrus (12-24 hours after signs of estrus appeared. The insemination conducted artificially using post-thawed Boer semen. The observed variables were non-return rate at day 21 post-insemination (NRR21), and pregnancy rate (PR) based on abdominal palpation diagnosis. The result showed that post-thawed Boer buck was qualified (sperm motility more than 40%) for artificial insemination. All of the Pote does were estrus after twice PGF2α intramuscular injection with 11 days intervals. The onset of estrus was 48-72 hours after the second injection PGF2α. The NRR21 and PR ranged from 66.66-100% and 37.50-66.66%, respectively. The NRR21 and PR of the T2 group were higher than those of the T1 group. It could be concluded that intracervical artificial insemination on Pote the does at mid-estrus were more effective, resulting in higher NRR21 and PR than those at early estrus.
Red dragon (Hylocereus polyrhizus) fruit peel extract increased the motility and viability of spermatozoa of hypercholesterolemic rats (Rattus norvegicus) Niken Meyliana Sari; M. Gandul Atik Yuliani; Eduardus Bimo Aksono Herupradoto; Nanik Hidayatik; Nurhusien Yimer; Tatik Hernawati
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 1 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i1.2023.34-42

Abstract

This study aims to determine the effect of red dragon (Hylocereus polyrhizus) fruit peel extract (RDFPE) on spermatozoa motility and viability of hypercholesterolemic rats (Rattus norvegicus) as a model. Twenty male rats were randomly divided into negative control (NC), positive control (PC), treatment 1 (T1), treatment 2 (T2), and treatment 3 (T3) groups. All rats were given 2 mL of high cholesterol feed orally every day for 28 days. On day-15, all rats were measured for their blood cholesterol levels, followed by treatment for 14 days. Rats in the NC, PC, T1, T2, and T3 groups were treated with 1% Na-CMC, Simvastatin 10 mg/kg BW, and RDFPE of 500, 750, and 1000 mg/kg BW, respectively. On day-29, all rats were sacrificed to evaluate spermatozoa viability and motility. The results showed that spermatozoa viability and motility in the hypercholesterolemic rats (NC) group were the lowest (p <0.05) among the groups. Treatment of hypercholesterolemic rats with Simvastatin 10 mg/kg BW (PC) group showed higher (p <0.05) spermatozoa viability and motility compared to the NC group. RDFPE dose of 1000 mg/kg BW (T3 group) resulted in higher (p <0.05) spermatozoa viability and motility compared to other RDFPE doses (T1 and T2) and the control (NC) groups, and it was similar (p >0.05) compared with the Simvastatin treated (PC) group. It could be concluded that the administration of 1000 mg/kg BW ethanolic extract of red dragon fruit (Hylocereus polyrhizus) peel increased the viability and motility of spermatozoa of hypercholesterolemic rats (Rattus norvegicus) which were the same as the group of rats given Simvastatin 10 mg/kg BW.
Effect of white guava (Psidium guajava L.) fruit juice on the quality of lead acetate induced rats (Rattus norvegicus) spermatozoa Alifia, Annisa; Sri Mulyati; Wurlina Wurlina; Tatik Hernawati; Budi Utomo; Soeharsono Soeharsono; Sri Agus Sudjarwo; Muhammad Thohawi Elziyad Purnama
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 3 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i3.2023.131-140

Abstract

This study aims to determine the effect of the administration of white guava (Psidium guajava L.) fruit juice on spermatozoa plasma membrane integrity (PMI), morphological abnormality, viability, and motility of lead acetate induced rats (Rattus norvegicus). Twenty-five male rats were divided into five groups: NC (negative control) group, rats were administered with distilled water twice daily at four-hour intervals; T0 (positive control) group, rats were administered daily with lead acetate 50 mg/kg bw and distilled water four hours later; T1, T2, and T3 groups, rats were administered daily with lead acetate 50 mg/kg bw and 0.5 mL of 25, 50, and 100% white guava fruit juice four hours later. The treatment of the rats was conducted for 14 days, and on day 15, all rats were sacrificed to assess the spermatozoa quality. Data was analyzed using ANOVA followed by Duncan's multiple range test at a confidence level of 95%. The results showed that exposure to lead acetate (T0) caused lower spermatozoa PMI, viability, and motility as well as higher spermatozoa morphological abnormalities (p <0.05) compared to those of the T0 group. Administration of white guava fruit juice starting at a dose of 25% (T1) resulted in higher spermatozoa motility, viability, and PMI as well as lower spermatozoa morphological abnormalities (p <0.05) compared to rats in the T0 group. It could be concluded that white guava fruit juice maintained the spermatozoa quality of lead acetate induced rats.
PENAMBAHAN PROTEIN INSULIN LIKE GROWTH FACTOR-I COMPLEX DALAM PENGENCER PEMBEKUAN SEMEN TERHADAP KUALITAS SPERMATOZOA KAMBING PADA WAKTU EKUILIBRASI Susilowati, Suherni; Hernawati, Tatik
Jurnal Kedokteran Hewan Vol 5, No 2 (2011): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v5i2.363

Abstract

Penelitian ini bertujuan mengisolasi protein Insulin Like Growth Factor-I (IGF-I) complex untukmeningkatkan kualitas semen beku kambing pada waktu ekuilibrasi setelah penambahan protein IGF-I complex. Penelitian ini terdiri atas 2 tahap yaitu isolasi protein IGF-I complex dari plasma seminal kambing dan aplikasi terhadap prosesing pembekuan semen. Pada tahap pertama dilakukan identifikasi IGF-I complex dengan menggunakan gel native polyacrylamide gel electrophoresis dan isolasi IGF-I complex. Pada tahap kedua dilakukanaplikasi penambahan protein IGF-I complex pada prosesing semen beku. Semen dikoleksi dengan menggunakan vagina buatan dan kemudian disentrifus selama 5 menit dengan kecepatan 1800 rpm. Kemudian semen dibagi menjadi tiga kelompok. Pada kelompok I, II, dan III ditambahkan protein IGF-I complex masing-masing 0, 12, dan 618 ng/ 3X10 sperma. Selanjutnya dilakukan ekuilibrasi selama 1 jam dan dilanjutkan dengan evaluasi motilitas, viabilitas, dan membran sperma. Hasil penelitian menunjukkan perbedaaan motilitas, viabilitas, dan membran sperma yang signifikan (P0,05) di antara tiga kelompok perlakuan. Dapat disimpulkan bahwa penambahan protein IGF-I complex dapat meningkatkan kualitas semen beku kambing pada fase ekuilibrasi.
Success rate of artificial insemination using sexed semen in dairy cows at the Setia Kawan dairy farmers cooperative, Pasuruan, Indonesia Tsaniya Atikahani Shabirah; Tri Wahyu Suprayogi; Tita Damayanti Lestari; Tatik Hernawati; Rimayanti Rimayanti; Sri Hidanah
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 3 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i3.2025.112-118

Abstract

This study aimed to evaluate the success rate of artificial insemination (AI) using sexed semen in dairy cows, based on the parameters of services per conception (S/C), conception rate (CR), and calf sex ratio. A descriptive method was employed, utilizing both primary and secondary data. Primary data were collected through structured interviews with farmers using questionnaires, while secondary data were obtained from institutional records of the Setia Kawan dairy farmers cooperative, located in Tutur District, Pasuruan Regency, East Java, Indonesia. A total sample of 250 dairy cows was selected, representing 15% of the 1,669 cows that had undergone AI with sexed semen. All sampled cows had at least one prior calving and were owned by members of the cooperative, with AI performed between 2021 and 2022. Artificial insemination was conducted by certified inseminators with over five years of experience, within 6-12 hours of estrus detection. The results showed an S/C value of 1.308 (327 inseminations for 250 cows) and a CR of 81.60% (204 pregnancies from 250 inseminations). Of the 250 calves born, 85.2% (213) were female and 14.8% (37) were male, resulting in a female-to-male sex ratio of 5.76:1. These findings suggest that AI using sexed semen can significantly improve both reproductive efficiency and the production of female offspring in dairy herds.
Comparative effects of epigallocatechin-3-gallate supplementation in skim milk, egg yolk-citrate, and combined extenders on the spermatozoa quality of Ettawah crossbred bucks during storage at 5 °C Muhammad Ikmal Robih; Anas Bad’rosana Prawindi; Tatik Hernawati; Budi Utomo; Suherni Susilowati; Tri wahyu Suprayogi; Sri Pantja Madyawati; Kadek Rachmawati; Mustofa Helmi Effendi; Yeni Dhamayanti
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 3 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i3.2025.147-158

Abstract

This study aimed to determine the quality of Ettawah crossbred buck semen diluted in skim milk, egg yolk-citrate extenders, or their combination, supplemented with epigallocatechin-3-gallate (EGCG). Semen was collected from Ettawah crossbred bucks using an artificial vagina. Six ejaculates were used for replication and divided into three extender treatments: T1, skim milk; T2, egg yolk-citrate; and T3, a combination of both, each supplemented with 1.5 µg/mL EGCG. The extended semen was stored in a refrigerator at 5 °C. Diluted samples were evaluated every 24 hours until spermatozoa motility declined to 30%, the minimum requirement for artificial insemination. The results showed that spermatozoa motility, viability, plasma membrane integrity, and morphological abnormality decreased during six days of storage at 5 °C. Spermatozoa motility remained above 30% for five days in T1 (36.50±1.22%) and T2 (41.67±2.06%), and for six days in T3 (43.33±1.03%). On day six, semen in the combined extender supplemented with EGCG (T3) showed significantly higher motility, viability, and membrane integrity, and lower morphological abnormalities (p <0.05) than the other treatments. In T2, all spermatozoa quality parameters were higher (p <0.05) than in T1. It can be concluded that the combined skim milk-egg yolk-citrate extender containing 1.5 µg/mL EGCG best maintained the spermatozoa quality of Ettawah crossbred bucks during six days of storage at 5 °C. Based on spermatozoa motility, the semen remained suitable for artificial insemination.
Co-Authors Abdul Samik Abdul Samik, Abdul Ageng Ilham Ramadhani Ahmad Hanif Azzam Ahmad Herdianto Wicaksono Aksono HP., Eduardus Bimo Al Arif, M Anam Al arif, Mohammad Anam Alifia, Annisa Amaliya, Anny Anas Bad’rosana Prawindi Ani Wijayanti Annisa Nur Fitria Rahma Anwar Ma'ruf Boedi Setiawan BUDI UTOMO Candika, Yossy Imam Cesaria, Mayrena Ayu Dadik Rahardjo Dadik Rahardjo, Dadik Dewi, Aprilia Kurnia Djoko Legowo Eka Pramyrtha Hestianah Emmanuel Djoko Poetranto Emy Koestanti Sabdoningrum Erlangga, Made Bagus Erma Safitri Estoepangestie, A.T.Soelih Ferdiansyah, Muhammad Billy Haditanojo, Wiyanto Hani Plumeriastuti Hardian, Andreas Bandang Herinda Pertiwi Hermadi, Herry Agus Hernanda, Ary Setya Herry Agoes Hermadi Hidanah, Sri IMAM MUSTOFA Imam Mustofa Indah Norma Triana, Indah Norma Intan Purwa Dewanti Ismudiono Ismudiono, Ismudiono Isti Annisa Ramahtia Iwan Sahrial Hamid Kadek Rachmawati Koesnoto Supranianondo Lilik Maslachah Listyorini, Linda Lucia Tri Suwanti, Lucia Tri M. Gandul Atik Yuliani Mas'ud Hariadi Meles, Dewa Ketut Meydilasari, Nanda Irsa Moh. Zaky ‘Ubaidillah Muhammad Ikmal Robih Muhammad Thohawi Elziyad Purnama Mustofa Helmi Effendi Nabila Ayu Saraswati Nanik Hidayatik Ngakan Made Rai Widjaja Niken Meyliana Sari Nove Hidajati Nurhusien Yimer Paramita L, Widya Pitaloka, Dinda Galuh Pramesti, Nugraheni Anggara Prastiya, Ragil Angga Prastyorini, Vina Ari Pudji Srianto Purnawan, Ahmad Budi Reisinta, Dinda Retno Sri Wahjuni Retta, Ayun Tria Marga Rimayanti Rimayanti Rochmah Kurnijasanti Rosyada, Zulfi Nur Amrina Safitri, Erma - Samosir, Sabrina Hatoguan Samuel Inioluwa Akeju Saputra, Rakan Mahiid Saputro, Amung Logam Sherina Lashita Candrakirana Soeharsono Soeharsono Sosilowati, Suherni Sri Agus Sudjarwo Sri Hidanah Sri Mulyati Sri Mulyati Sri Pantja Madyawati Sri Pantja Madyawati, Sri Pantja Suherni Susilowati Sulistyowati, Deny Supranianondo, Koesnoto Supriyadi Supriyadi Supriyadi Tita Damayanti Lestari Tjuk Imam Restiadi Tri Nurhajati, Tri Tri Wahyu Suprayogi Trilas Sardjito Tsaniya Atikahani Shabirah Utama, Dirga Januar Surya Vladika, Maulinda Agchirilia Warsito, Sunaryo Hadi Wawid Purwatiningsih Widiantoro, Krisna Widiyatno, Thomas V. Widya Paramita Lokapirnasari Wurlina, W Yeni Dhamayanti Yudhana, Aditya Yudit Oktanella, Yudit