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All Journal Jurnal Veteriner Jurnal Medik Veteriner Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Mitra Mahajana: Jurnal Pengabdian Masyarakat Jurnal Kedokteran Hewan Jurnal Agro Veteriner

Tatik Hernawati

Department Of Veterinary Reproduction, Faculty Of Veterinary Medicine, Universitas Airlangga

Author-ID : 3693289

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Effect of Addition of Green Tea Extract (Camellia sinensis) in Egg Yolk Tris Diluter on Spermatozoa Quality in Bali Cattle (Bos sondaicus) After Freezing Wijayanti, Ani; Suprayogi, Tri Wahyu; Prastiya, Ragil Angga; Hernawati, Tatik; Sardjito, Trilas; Saputro, Amung Logam; Amaliya, Anny; Sulistyowati, Deny
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.66-74

This study aimed to determine the effect of adding 96% ethanol extract of green tea (Camellia sinensis) in egg yolk tris diluter on the quality of spermatozoa of Bali cattle (Bos sondaicus) after freezing. The samples were divided into four different treatment groups, which contained semen sample + 13% glycerol + each egg yolk tris diluter + green tea extract (P0) 0 mg/100 ml, (P1) 0,05 mg/100 ml, ( P2) 0,10 mg/100 ml, and (P3) 0,15 mg/100 ml. The variables observed included motility, viability, and spermatozoa abnormalities. Data were analyzed using OneWay ANOVA and continued with Duncan's test (p<0,05). The results showed that the P3 group had motility of 49,00 ± 2,15, viability of 61,92 ± 1,70, and abnormality of 4,24 ± 2,16 significantly compared to the other groups. In conclusion, the P3 group with the addition of 0,15 mg/100 ml of green tea could increase the motility, viability, and abnormal spermatozoa of Bali cattle.

The addition of duck egg yolk in skim milk – fructose extender maintains sperm motility and viability of Muscovy duck (Cairina moschata) at 5°C storage Isti Annisa Ramahtia; Tatik Hernawati; Tri Wahyu Suprayogi; Tjuk Imam Restiadi; Djoko Legowo; Samuel Inioluwa Akeju
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.1-8

This study aimed to determine the addition of duck egg yolk in skim milk – fructose extender on sperm motility and viability of Muscovy duck (Cairina moschata) at 5°C storage. Ejaculates of Muscovy duck were divided into four portions equally. The first portion was for the control group (diluted in skim milk – fructose extender), and the second, third, and fourth portions were for the treatment groups of T1, T2, and T3 (diluted in skim milk – fructose extender added with 2.5, 5.0, and 7.5% v/v duck egg yolk). Sperm motility and viability were evaluated two hourly until the motility percentage reached 40%. The data were analyzed using ANOVA and Duncan test. The result showed that the highest percentage of spermatozoa motility and viability (p<0.05) was in the T2 group compared to the other groups. It could be concluded that the Muscovy duck semen extended in skim milk - fructose with 2.5 to 7.5% egg yolk of duck qualified for artificial insemination at storage up to 10 hours in 5°C storage.

The effect of ciplukan (Physalis angulate Linn.) leaf extract on the testicles of rats (Rattus norvegicus) exposed to heat Annisa Nur Fitria Rahma; Sherina Lashita Candrakirana; Rimayanti Rimayanti; Tri Wahyu Suprayogi; Budi Utomo; Tatik Hernawati; Anwar Ma'ruf; Nove Hidajati; Kadek Rachmawati; Eka Pramyrtha Hestianah; Hani Plumeriastuti
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.30-38

This study aims to determine the effect of administration of ciplukan (Physalis angulate Linn.) leaf extract on the variables of testicular weight, seminiferous tubules diameter, and spermatozoa plasma membrane integrity of rats (Rattus norvegicus) exposed to heat as model animals. Rats were divided randomly into five groups. In the NC group, rats were not exposed to heat and were only given 1% Na-CMC. In the PC, T1, T2, and T3 rats were exposed to heat followed by administration of 1% Na-CMC, 100, 200, and 400 mg/kg bw of ciplukan leaf extract (CLE) in 1% Na-CMC. Heat exposure at 40°C was conducted for 60 minutes every day for 21 days. On day-22, all rats were sacrificed for testicular evaluation. The results showed that all variables in the PC group were lower (p <0.05) than in the NC group. All variables in the T3 group were higher (p <0.05) than in the PC group. Testicular weight and spermatozoa plasma membrane integrity in the T3 group were not significantly different (p >0.05) from the NC group. However, the diameter of the seminiferous tubules in the T3 group were lower (p <0.05) than in the NC group. This study revealed that the administration of ciplukan leaf extract as an antioxidant increased testicular weight, seminiferous tubules diameter, and spermatozoa with intact plasma membrane in rats exposed to heat.

Coconut water combined with purebred chicken egg yolk as an alternative semen extender for Madura Pote bucks semen preservation Tatik Hernawati; Susilowati, Suherni; Tri Wahyu Suprayogi; Widya Paramita Lokapirnasari; Zulfi Nur Amrina Rosyada
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 2 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i2.2024.96-104

One of the many efforts to increase the quality of livestock genetics is through artificial insemination (AI). Other than increasing it, AI can be conducted to preserve semen. A successful AI is determined by seminal quality, therefore, a method to preserve semen for a longer storage time is needed. The method used is adding an extender that fulfills prerequisites for a semen extender such as coconut water combined with egg yolk citrate extender. Coconut water is rich in carbohydrates, protein, vitamins, and antioxidants while egg yolk contains lecithin. This study aims to find out the Pote buck spermatozoa quality stored in coconut water and egg yolk extender. This study uses three groups of treatments (T0: 0.1 ml semen + 0.9 ml egg yolk citrate, T1: 0.1 ml semen + 0.9 ml coconut water, and T2: 0.1 ml semen + egg yolk citrate (20%) + coconut water). All three of these are stored at 5oC and evaluated every day until day 5 of their motility, viability, intact plasma membrane, abnormality, and MDA level. Data analysis used is ANOVA and a further test called BNT is conducted if a significant difference is determined. No significant difference was found between T0 and T1 (p>0.05). The highest progressive motility, viability, and intact plasma membrane (%) among the three groups of treatments happened to be from T2. Meanwhile, a low percentage of spermatozoa abnormality and MDA level were also found in T2 with its extender being coconut water combined with egg yolk citrate. To conclude, the best extender for storing Pote buck semen is stored at 5oC is coconut water combined with egg yolk citrate extender.

Potential of fermented rumen content of cow feed on daily egg productivity and quality of yolk color of quail egg (Cortunix cortunix japonica) Moh. Zaky ‘Ubaidillah; Mohammad Anam Al Arif; Tatik Hernawati; Koesnoto Supranianondo; Emy Koestanti Sabdoningrum; Widya Paramita Lokapirnasari
Jurnal Agro Veteriner Vol. 8 No. 1 (2024): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v8i1.64980

The purpose of this study was to determine the effect of fermented rumen contents on quail rations. The parameters measured were daily egg production and egg yolk quality. There are 96 quails with 4 treatments and 6 repetitions. (T0) formulation feed without fermented rumen contents, (T1) formulation feed + 5% fermented rumen content, (T2) formulation feed + 10% fermented rumen content, (T3) formulation feed + 15% fermented rumen content. Samples are observed daily for daily egg production and the last week for egg yolk quality. The results showed that the uses of fermented rumen contents in rations at a rate of up to 15% causeddid not interfere towards daily egg production and could even improve the quality of egg yolk.

Spermatozoa motility and viability of turkey (Meleagris gallopavo) semen in egg yolk nanoparticles-phosphate buffered saline extender at 3-5°C storage Nabila Ayu Saraswati; Herry Agoes Hermadi; Tri Wahyu Suprayogi; Tatik Hernawati; Eka Pramyrtha Hestianah
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 3 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i3.2024.129-138

This study aimed to evaluate the spermatozoa motility and viability of turkey semen extended with egg yolk nanoparticles-PBS extender at various concentrations, stored at 3-5°C. Semen was collected from two turkeys and divided into four treatment groups. Group T0 consisted of fresh turkey semen extended with 20% (v/v) fresh egg yolk in phosphate-buffered saline (PBS). Groups T1, T2, and T3 consisted of fresh turkey semen extended with 5, 10, and 20% (v/v) egg yolk nanoparticles in PBS, respectively. Extended semen was stored in a refrigerator (3-5°C), and progressive spermatozoa motility and viability were evaluated every two hours until spermatozoa motility decreased to 40%. The results showed that turkey semen in T3 extender containing 20% egg yolk nanoparticles in PBS was able to maintain spermatozoa motility for up to eight hours, better than T0 and T2 extenders which supported spermatozoa motility for six hours. In contrast, T1 extenders only support spermatozoa motility for four hours. T3 extenders were also able to maintain spermatozoa viability for up to eight hours, while T0, T1 and T2 extenders could only support viability for 6 hours. The extender with 20% egg yolk nanoparticles in PBS was significantly better (p <0.05) in maintaining spermatozoa quality compared to those with 5 and 10% egg yolk nanoparticles, as well as the extender with 20% fresh egg yolk. In conclusion, 20% egg yolk nanoparticles in PBS semen extender was effective in maintaining the motility and viability of turkey (Meleagris gallopavo) spermatozoa stored at 3-5°C for up to eight hours.

PEMBERDAYAAAN PETERNAKAN KAMBING POTE MELALUI TEKNIK KAWIN SUNTIK UNTUK PENINGKATAN EKONOMI MASYARAKAT BANGKALAN Sosilowati, Suherni; Hernawati, Tatik; Suprayogi, Tri Wahyu; Candika, Yossy Imam; Supriyadi, Supriyadi
Mitra Mahajana: Jurnal Pengabdian Masyarakat Vol. 5 No. 3 (2024): Volume 5 Nomor 3 Tahun 2024
Publisher : LPPM Universitas Flores

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/mahajana.v5i3.4556

Community Service was conducted to increase local people’s economy by Poté goat population using Artificial Insemination in Alaskokon, Modung, and Bangkalan. The partner in this Community Service are local Poté goat farmers in Alaskokon village, Modung, Bangkalan. The analysis of this situation shows a problem that the Partner has been facing. Even though the Poté population is not lacking, their rearing is conventional where it is not focused on selling but more on saving for when there is no money (Rojokoyo). To tackle this problem, Artificial Insemination was introduced to the Poté. Moreover, socialisation was done by covering various aspects. Sosialisation was given on caring for pregnant does, semen collection using an artificial vagina, and buck semen dilution using different types of extenders such as coconut water, milk, and egg yolk-citrate. Socialisation of the marketing strategy for goats was also given. Artificial Insemination Technology is a solution for improving the goat population. Goats are fast-bred livestock with a relatively short gestation time and can utilise forage like grasses. Artificial Insemination technique can be administered three times in two years to increase the goat population, thus, the local people’s economy may follow. After the programme was completed, farmers were enthusiastic about collecting goat semen and inseminating livestock.

COUNSELING ON REPRODUCTIVE DISORDERS MANAGEMENT IN FEMALE BEEF CATTLE LIVESTOCK GROUPS IN DALEGAN VILLAGE, PANCENG DISTRICT, GRESIK REGENCY Suprayogi, Tri Wahyu; Susilowati, Suherni; Hermadi, Herry Agoes; Al Arif, M Anam; Hernawati, Tatik; Mulyati, Sri; Suwanti, Lucia Tri; Maslachah, Lilik; Kurnijasanti, Rochmah; Lestari, Tita Damayanti; Effendi, Mustofa Helmi; Hernanda, Ary Setya; Supriyadi; Saputra, Rakan Mahiid; Dewi, Aprilia Kurnia
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 9 No. 1 (2025): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v9i1.2025.026-038

Management of reproductive disorders in female beef cattle (Bos taurus) is a vital step in enhancing livestock productivity and animal welfare. This educational activity was conducted in Dalegan Village, Panceng District, Gresik Regency, aiming to assist farmers in improving cattle reproductive performance through feed technology and supporting the development of modern farming practices. The methods applied included counseling, practical field demonstrations, and interactive discussions with participants, presented in a qualitative descriptive format. The results indicated an improvement in farmers' knowledge of the causes, clinical signs, and management steps for reproductive disorders such as anestrus, dystocia, and endometritis, as evaluated through pre-test and post-test assessments. Additionally, the activity provided guidance on the importance of feed management, cattle reproductive health, and modern farm management. In conclusion, this counseling successfully increased farmers' awareness of cattle reproductive health, which is expected to support the productivity of beef cattle farming in the region.

PENGARUH PEMBERIAN EKSTRAK KULIT BAGIAN DALAM SEMANGKA (Citrullus lanatus) TERHADAP KEUTUHAN MEMBRAN PLASMA DAN ABNORMALITAS MORFOLOGI TIKUS JANTAN (Rattus norvegicus) SETELAH DIPAPAR SUHU PANAS Reisinta, Dinda; Paramita L, Widya; Hernawati, Tatik; Meles, Dewa Ketut; Madyawati, Sri Pantja; Suprayogi, Tri Wahyu
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

This study aims to determined the effect of watermelon's inner rind extract (Citrullus lanatus) towards the integrity of the plasma membrane and morphological abnormalities of male Norway rats's spermatozoa (Rattus norvegicus) after being exposed to hot temperature. The content of lycopene in the inner rind of watermelon serves an antioxidant counteracting free radicals. A total of 20 male Norway rats (Rattus norvegicus) were used in this study with an average age of 2 months. The study was divided into five treatments and four replications, consisting of: K(-) as negative control given CMC Na 0.5% as much as 0.5 ml, P0 as positive control was exposed to 40° C for 1 hour and CMC Na 0.5% of 0.5 ml. P1, P2 and P3 are exposed to 40° C for 1 hour and inner rind extracts of watermelon with a multilevel dose of P1: 20mg/head/day, P2: 40mg/head/day, and P3: 80mg/head/day that each dose was dissolved in CMC Na 0.5% of 0.5 ml. The results showed that giving inner rind extract of watermelon to the rats increased the integrity of the plasma membrane and decreased morphological abnormalities of male rat's spermatozoa (Rattus norvegicus). The result of increased the integrity of the plasma membrane and highest decreased morphological abnormality was shown by P3 group given a dose of 80 mg/head/day.

EFISIENSI REPRODUKSI SAPI PERANAKAN LIMOUSIN AKSEPTOR INSEMINASI BUATAN DI KECAMATAN TIKUNG, KABUPATEN LAMONGAN TAHUN 2016 Prastyorini, Vina Ari; Supranianondo, Koesnoto; Safitri, Erma; Restiadi, Tjuk Imam; wurlina, wurlina; Hernawati, Tatik
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

The purpose of this study was known the difference of reproductive performans Limousine Cross Breed that haved artificial insemination from diffrence straw from BBIB Singosari and BBIB Lembang covering Conception Rate, Service per Conception, and Calving Rate in period 2016th. This study uses survey research which data from primary and secondary. Primary data were obtained from interview with Limousine Cross Breed breeders and secondary data were obtained from artificial insemination officer's record. The observed variables are Conception rate (CR), Service per conception (S/C) and Calving rate (CvR). Data were analyzed descriptive that average and Chi Square.Result of research showed no significant difference (p>0.05) on CR, S/C and CvR between Limousin cross breed cows that haved artificial insemination from BBIB Singosari and BIB Lembang.

Co-Authors Abdul Samik Abdul Samik, Abdul Ageng Ilham Ramadhani Ahmad Hanif Azzam Ahmad Herdianto Wicaksono Aksono HP., Eduardus Bimo Al Arif, M Anam Al arif, Mohammad Anam Alifia, Annisa Amaliya, Anny Anas Bad’rosana Prawindi Ani Wijayanti Annisa Nur Fitria Rahma Anwar Ma'ruf Boedi Setiawan BUDI UTOMO Candika, Yossy Imam Cesaria, Mayrena Ayu Dadik Rahardjo Dadik Rahardjo, Dadik Dewi, Aprilia Kurnia Djoko Legowo Eka Pramyrtha Hestianah Emmanuel Djoko Poetranto Emy Koestanti Sabdoningrum Erlangga, Made Bagus Erma Safitri Estoepangestie, A.T.Soelih Ferdiansyah, Muhammad Billy Haditanojo, Wiyanto Hani Plumeriastuti Hardian, Andreas Bandang Herinda Pertiwi Hermadi, Herry Agus Hernanda, Ary Setya Herry Agoes Hermadi Hidanah, Sri IMAM MUSTOFA Imam Mustofa Indah Norma Triana, Indah Norma Intan Purwa Dewanti Ismudiono Ismudiono, Ismudiono Isti Annisa Ramahtia Iwan Sahrial Hamid Kadek Rachmawati Koesnoto Supranianondo Lilik Maslachah Listyorini, Linda Lucia Tri Suwanti, Lucia Tri M. Gandul Atik Yuliani Mas'ud Hariadi Meles, Dewa Ketut Meydilasari, Nanda Irsa Moh. Zaky ‘Ubaidillah Muhammad Ikmal Robih Muhammad Thohawi Elziyad Purnama Mustofa Helmi Effendi Nabila Ayu Saraswati Nanik Hidayatik Ngakan Made Rai Widjaja Niken Meyliana Sari Nove Hidajati Nurhusien Yimer Paramita L, Widya Pitaloka, Dinda Galuh Pramesti, Nugraheni Anggara Prastiya, Ragil Angga Prastyorini, Vina Ari Pudji Srianto Purnawan, Ahmad Budi Reisinta, Dinda Retno Sri Wahjuni Retta, Ayun Tria Marga Rimayanti Rimayanti Rochmah Kurnijasanti Rosyada, Zulfi Nur Amrina Safitri, Erma - Samosir, Sabrina Hatoguan Samuel Inioluwa Akeju Saputra, Rakan Mahiid Saputro, Amung Logam Sherina Lashita Candrakirana Soeharsono Soeharsono Sosilowati, Suherni Sri Agus Sudjarwo Sri Hidanah Sri Mulyati Sri Mulyati Sri Pantja Madyawati Sri Pantja Madyawati, Sri Pantja Suherni Susilowati Sulistyowati, Deny Supranianondo, Koesnoto Supriyadi Supriyadi Supriyadi Tita Damayanti Lestari Tjuk Imam Restiadi Tri Nurhajati, Tri Tri Wahyu Suprayogi Trilas Sardjito Tsaniya Atikahani Shabirah Utama, Dirga Januar Surya Vladika, Maulinda Agchirilia Warsito, Sunaryo Hadi Wawid Purwatiningsih Widiantoro, Krisna Widiyatno, Thomas V. Widya Paramita Lokapirnasari Wurlina, W Yeni Dhamayanti Yudhana, Aditya Yudit Oktanella, Yudit

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