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CHARACTERISTIC OF SKIN MORPHOLOGY OF SUNDA PORCUPINE (Hystrix javanica) WITH SPECIAL REFERENCE TO THE CONNECTIVE TISSUE Andhika Yudha Prawira; Desrayni Hanadhita; Anisa Rahma; Supratikno Supratikno; Savitri Novelina; Srihadi Agung Priyono
Jurnal Kedokteran Hewan Vol 12, No 1 (2018): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i1.6892

Abstract

This study was conducted to investigate the histological characteristic, type, and distribution of connective tissue in Sunda porcupine skin. The investigation was carried out in three adult of sunda porcupines at microscopic level using hematoxylin eosin, Masson thrichrome, Verhoeffs van Gieson, alcian blue pH 2.5 and periodic acid Schiff staining methods. Skin consists of epidermis, dermis hypodermis, and subcutaneous muscle. Quill follicles were the main and dominant structure as well as the specific characteristic on Sunda porcupine skin. The connective tissue was distributed well in basal membrane, dermis, quill follicle, and hypodermis with various intensity and density. The collagen was the main fiber found in the skin while the elastin fiber was not observed. The acid carbohydrate was found distributed well in the skin while the neutral carbohydrate was not detected in this study. In addition the fibers of connective tissue associated with the adipose tissue which found plentifully in quill follicles and hypodermis. The present results showed that the wide distribution of connective tissue might have an important role on the wound healing physiology of Sunda porcupine skin.
Microscopical Study of Pancreatic Tissue of Babirusa (Babyrousa babyrussa) Using Conventional and Immunohistochemical Methods Ketut Mudite Adnyane; Alastair A Macdonald; Srihadi Agungpriyono
Jurnal Kedokteran Hewan Vol 4, No 2 (2010): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v4i2.3083

Abstract

This study utilized conventional and immunohistochemical methods, to describe the morphology and distribution of glucagon and insulin cells in the pancreas of adult male babirusa (Babyrousa babyrussa). The results of research showed that the endocrine portion (Langerhans islet) spread evenly on the acinar portion of pancreas. Observation using immunohistochemical method showed that glucagon cells were distributed on the perifer, whereas insulin cells in the center of the Langerhans islet of pancreas. In general, the number of insulin cells was higher than glucagon cells. It is interesting to note, in the pancreas babirusa also found that neonatal elements similar to the Langerhans islet, but have larger sizes (ranging between 200 -700 mm). The picture is similar to the large Langerhans islet was reported in ruminant.  These cells give a negative reaction to insulin and glucagon antisera.
KARAKTERISTIK MORFOLOGI ESOFAGUS DAN LAMBUNG BANDIKUT (Echymipera kalubu) (Esophagus and Stomach Morphological Characteristic of Bandicoot Echymipera kalubu (Marsupialia: Peroryctidae)) Ursula Paulawati Maker; Chairun Nisa; Srihadi Agungpriyono
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5043

Abstract

The morphological characteristics of the stomach and esophagus of five adults bandicoot (Echymipera kalubu) with average 1.16±0.29 kg in body weight and 38.2±4.76 cm in body length were studied macroscopically, microscopically and histochemically. The bandicoot esophagus were found relatively long about one third of body length. The esophageal glands were identified along the esophagus and the number decreased gradually from cranial to caudal region. In addition of smooth muscle, skeletal muscles were also examined in the external muscle layer of esophagus up to the esophageal junction. The stomach of the E. Kalubu had short lesser curvature and three glandular regions of cardiac, fundic and pyloric glands were observed, respectively. The cardiac glands area was small and concentrated in the cranial part of the stomach. The fundic glands area occupied about half parts of the stomach. Mucous surface epithelial cells, mucous neck cells, parietal cells and chief cells were found in the entire fundic gland. The parietal cells were the most abundant cells in the major curvature distributed from the basal to neck area of the glands, but few in the minor curvature. Chief cells were mostly distributed in the basal gland. The pyloric glands region was observed in caudal part of the stomach. Staining with alcian blue-periodic acid Schift (AB-PAS) showed various distribution and intensity of neutral and acid mucopolysaccharides in the esophageal and stomach mucosa. High concentration of neutral and acid mucopolysaccharides were detected in esophageal and stomach glands with various concentration. Morphological characteristics of the esophagus and stomach were assumed to be related to the digestive processes in the gastrointestinal tract of bandicoot.
IDENTIFIKASI GOLONGAN DARAH DAN KEMUNGKINAN HUBUNGANNYA DENGAN WARNA RAMBUT PADA KUCING DOMESTIK INDONESIA (Felis familiaris) Gunanti g; Dwi Endrawati; Helny Rosita Supriadi; Riki Siswandi; Srihadi Agungpriyono
Jurnal Kedokteran Hewan Vol 7, No 1 (2013): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i1.571

Abstract

Penelitian ini bertujuan mengetahui distribusi penyebaran golongan darah A, B, dan AB dan mengetahui kemungkinan hubungan antara golongan darah dengan kemunculan pola warna rambut pada kucing domestik di Indonesia. Pada penelitian ini digunakan 50 ekor kucing domestik yang berasal dari daerah Bogor dan Bekasi. Pemeriksaan golongan darah kucing dilakukan dengan identifikasi golongan darah dengan kit antibodi monoklonal Shigeta(Jepang). Hasil penelitian menunjukkan bahwa semua kucing domestik yang diteliti bergolongan darah A dan tidak diperoleh tanda-tanda adanya hubungan antara golongan darah dengan warna rambut. Terdapat sembilan kemunculan warna rambut yang terdiri atas warna tunggal, kombinasi dua warna, dan kombinasi tiga warna.
Morphological Study of the Oesophagus and Stomach of the Cave Swiflets (Collocalia linchi) Savitri Novelina; Evalina E; Aryani S Satyaningtijas; Srihadi Agungpriyono; Heru Setijanto
Jurnal Kedokteran Hewan Vol 3, No 1 (2009): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v3i1.3080

Abstract

This study was conducted with aim to tend out morphology of oesophagus and stomach of the cave swiflets (Collocalia linchi) at macroscopic and microscopic levels.  The data revealed that esophagus was 2.93 cm in length and possessed no crop.  The stomach was small and the isthmus was not clear. Mucosa of the esophagus was lined by stratified squoamus epithelium. Esophageal gland was mucous type. The glands were well developed and distributed along the esophagus. The external muscle layer consisted of inner circular and outer longitudinal layers.  The stomach could be distinguished into proventriculus and ventriculus with no clear isthmus between them. The mucosa of proventriculus was lined by single columnar epithelium. The ventricular gland area was divided into cardiac, fundic  and pyloric gland areas. The surface of ventriculus was lined with cuticula. In general the esophagus and stomach of the cave swiftlets were simple with no crop in the esophagus and isthmus in the stomach. These might be related with the kind of food and feeding behavior of this species.
CHARACTERIZATION OF GLYCOCUNJUGATE DISTRIBUTION IN THE EPIDIDYMIS OF THE JAVAN MUNTJACS (Muntiacus muntjak muntjak) Sri Wahyuni; Hamny Sofyan; Hafizuddin Hafizuddin; I Ketut Mudite Adnyane; Gholib Gholib; Muhammad Jalaluddin; Fadli A. Gani; Mulyadi Adam; Tongku Nizwan Siregar; Srihadi Agungpriyono
Jurnal Kedokteran Hewan Vol 16, No 1 (2022): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v16i1.24418

Abstract

The presence of glycoconjugates in caput, corpus, and cauda epididymis of the Javan muntjacs was performed using lectin histochemical method. The epididymis organs were obtained from two adult male Javan muntjacs in hard antler stage and then immediately fixed in Bouin’s solution and processed in histological manner. Six types of biotinylated lectins namely Peanut agglutinin (PNA), Soybean agglutinin (SBA), Wheat germ agglutinin (WGA), Ricinus communis agglutinin (RCA), Concanavalin A (Con A), and Ulex europaeus agglutinin I (UEA I) were applied to detect the specific glycoconjugates in the epididymal tissue of muntjacs. The result showed the presence of glycoconjugates were detected by five types of lectins except UEA I in caput, corpus, and cauda epididymis with specific distribution and also the intensity of lectins binding. Glycoconjugates β-galactose, β-glucose, mannose, N-acetyl-galactosamine, N-acetyl-glucosamine, and sialic acid were stained with various intensity in epithelial cells, basal cells, stereocilia, spermatozoa, and stroma of caput, corpus, and cauda epididymis. The presence of sialic acid on the surface of spermatozoa detected by WGA are thought to be play a role on sperm protection during their maturation and transit along epididymal lumen. In conclusion, the difference of glycoconjugates distribution pattern and lectin binding in the epididymal caput, corpus, and cauda of the Javan muntjac indicates the regional differences in epididymal function in spermatozoa maturation.
SEBARAN GLYCOCONJUGATE PADA SEL EPITEL OVIDUK KANCIL (Tragulus javanicus) Hamny H; Srihadi Agungpriyono; Ita Djuwita; Sri Wahyuni; Wahono Esthi Prasetyaningtyas; Idawati Nasution; Savitri Novelina
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i2.2623

Abstract

Penelitian ini bertujuan mengetahui distribusi glycoconjugate yang terekspresi pada sel epitelium oviduk kancil (Tragulus javanicus). Dalam penelitian ini digunakan satu oviduk kancil yang berasal dari satu ekor kancil b etina dewasa berumur lebih dari satu tahun. Sampel difiksasi dengan larutan Bouin dan diproses menurut standar histologi sampai menjadi blok parafin dan dipotong dengan ketebalan 5 µm. Jenis lektin yang digunakan adalah biotinylated (Con A, PNA, RCA, UEA I, dan WGA) dengan dosis masing-masing sebanyak 15 µg/ml. Hasil penelitian diketahui bahwa glycoconjugate dengan residu gula galaktosa, glukosa, manosa, N-asetilgalaktosamin, N-asetilglukosamin, fukosa, dan asam sialat ditemukan pada bagian apikal sel epitel dan di dalam sitoplasma. Glycoconjugate dengan residu gula N-asetilgalaktosamin merupakan glycoconjugate yang paling banyak ditemukan di bagian apikal sel epitel dan di dalam sitoplasma dibandingkan dengan glycoconjugate dengan residu gula lainnya.
STUDI HISTOKIMIA LEKTIN PADA SEL-SEL SPERMATOGENIK TESTIS MUNCAK (Muntiacus muntjak muntjak) (Lectin Histochemical Study of Testicular Spermatogenic Cells in Muntjak (Muntiacus muntjak muntjak)) Sri Wahyuni; Srihadi Agungpriyono; I. Ketut Mudite Adnyane; Hamny Hamny; Muhammad Jalaluddin; Gholib Gholib; Muslim Akmal; Mulyadi Adam; Dwinna Aliza; Tongku Nizwan Siregar
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i1.3396

Abstract

The objective of this study was to identify the type of specific glycoconjugates and its distribution in testicular spermatogenic cells in muntjak (Muntiacus muntjak muntjak) based on lectins histochemistry. An adult male muntjak aged 4-5 years old in hard antler period was used in this study. Testicular tissue was fixed in Bouin solution and processed histologically. Histochemistry method was performed using six types biotinylated lectins such as peanut agglutinin (PNA), soybean agglutinin (SBA), wheat germ agglutinin (WGA), ricinus communis agglutinin (RCA), concanavalin A (Con A), and ulex europaeus agglutinin I (UEA I) with 20 µg/ml of concentration for PNA lectins and 15µg/ml for other type of lectins. The results showed that glycoconjugates were detected by all type of lectins except UEA I in testicular spermatogenic cells with variation in distribution pattern and also the intensity of lectins binding. Glycoconjugates β-galactose, β-glucose, mannose, Nacetylgalactosamine, N-acetylglucosamine and sialic acid were stained intensely by lectins in golgy-cap phase and acrosomal phase of spermatids. Glycoconjugate N-acetylgalactosamine was the sugar residues which distributed abundantly that marked by positive reaction with PNA, SBA, and RCA lectins. In conclusion, glycoconjugates are detected in testicular spermatids cells of muntjak indicated that glycoconjugates have an important role in spermatogenesis particularly in spermiogenesis. Key words: glycoconjugates, lectins, spermatid, spermatozoa, muntjak
MORFOLOGI DAN MORFOMETRI PERTUMBUHAN RANGGAH VELVET MUNCAK JANTAN (Muntiacus muntjak muntjak) Sri Wahyuni; Srihadi Agungpriyono; Muhammad Agil; Tuty Laswardi Yusuf
Jurnal Kedokteran Hewan Vol 5, No 1 (2011): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v5i1.419

Abstract

Penelitian ini bertujuan menggambarkan morfologi pertumbuhan ranggah velvet (RV) dua ekor muncak jantan (M1 dan M2) yang dipelihara di kandang penelitian secara terpisah. Tanggal lepas ranggah dan hari pertama percabangan RV serta awal pengelupasan kulit velvet diamati dan dicatat. Pengukuran meliputi panjang dan diameter ranggah velvet utama (RVU) dan ranggah velvet cabang (RVC) dexter et sinister, serta durasi pertumbuhan RV. Rataan ± SD panjang dan diameter maksimum RVU dan RVC muncak M1 adalah 148,25±1,12 mm; 46,26±0,68 mm (panjang), 19,21±2,35 mm; 9,12±0,21 mm (diameter) dengan durasi pertumbuhan 104 hari; sedangkan muncak M2 berturut-turut adalah: 146,44±1,58 mm; 46,94±1,97 mm (panjang); 13,65±1,27 mm; 8,49±0,29 mm (diameter) dengan durasi pertumbuhan 98 hari. Tidak terdapat perbedaan tahapan pertumbuhan RVU dan RVC antara muncak M2 dan M1, namun berbeda pada ukuran RV dan durasi pertumbuhannya. Muncak M2 memasuki ranggah keras setelah pengelupasan kulit velvet lebih cepat dibandingkan M1. Dapat disimpulkan bahwa ukuran pertumbuhan RV berkorelasi erat dengan faktor umur, berat badan, dan postur tubuh muncak jantan.
Karakteristik Morfologi Hati Ayam Cemani (Gallus gallus domesticus) Putri Syifa Camilla; Nurhidayat; Heru Setijanto; Supratikno; Chairun Nisa; Srihadi Agungpriyono; Danang Dwi Cahyadi; Dedi Rahmat Setiadi; Savitri Novelina
Jurnal Veteriner dan Biomedis Vol. 1 No. 2 (2023): September
Publisher : Sekolah Kedokteran Hewan dan Biomedis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jvetbiomed.1.2.77-83.

Abstract

Tujuan penelitian ini adalah mempelajari morfologi hati ayam cemani (Gallus gallus domesticus) secara makroanatomi dan mikroanatomi. Penelitian ini menggunakan organ hati dari tiga ekor ayam cemani betina. Pengamatan makroanatomi untuk mempelajari morfometri yang meliputi panjang, lebar, tebal, dan berat organ hati. Pengamatan mikroanatomi dilakukan dengan menggunakan pewarnaan Haematoxylin-Eosin, untuk mengamati morfologi sel hati. Data yang diperoleh dianalisis secara deskriptif. Hasil dari pengamatan makroanatomi menunjukkan warna hati adalah merah kecoklatan dengan rata – rata bobot hati sebesar 19.3±2.5 gram. Pengamatan mikroanatomi menunjukkan hati diselaputi oleh jaringan ikat longgar pada permukaannya, kemudian terdapat kapsula Glisson. Di setiap lobulus hati terdapat vena centralis, cabang dari vena porta hepatica, cabang dari arteri hepatica, dan ductus choledochus. Parenkim hati terdiri dari hepatosit dan sinusoid. Sel – sel non parenkim yang terdapat di hati adalah sel Kupffer dan sel endotel. Sel pigmen melanin pada parenkim hati ditemukan dalam jumlah yang sedikit, sebagian besar pigmen melanin terdapat di sekitar vena porta, arteri hepatica, dan ductus choledochus.
Co-Authors A.S. Satyaningtijas Adi Winarto Adrien Jems Akiles Unitly Afiff , Usamah Alastair A Macdonald Amaq Fadholly Amrozi Anak Agung Istri Sri Wiadnyani Andhika Yudha Prawira Andhika Yudha Prawira Andhika Yudha Prawira Andhika Yudha Prawira Andhika Yudha Prawira Andhika Yudha Prawira Angelina N. Tethool Anisa Rahma Arief Boediono Aries Boediono Aryani Sismin Sastyaningtijas Asri Pudjirahaju Auzi Asfarian Chairun Nisa Chairun Nisa Chairun Nisa' Chairun Nisa’ Chairun Nisa’ Chairun Nisa’ Cutnya’ Shaliran Nazlie (Alm) Damiana Rita Ekastuti Danang C. Cahyadi Danang Dwi Cahyadi Dedi Rahmat Setiadi Deni Noviana Denny W. Lukman Desrayni Hanadhita Desrayni Hanadhita Desrayni Hanadhita Desrayni Hanadhita Desrayni Hanadhita Dondin Sajuthi Dwi Endrawati Dwi Kesuma Sari Dwinna Aliza Eragilang Muhammad Hastapatria Erdiansyah Rahmi Erni Sulistiawati Etih Sudarnika Etty Riani Evalina E Fadli A. Gani Gholib Gholib GONO SEMIADI Gunanti . Hafizuddin Hafizuddin Halim Bakti Harjo Hamny Sofyan Hany Savitry Hellen Kurniati, Hellen Helny Rosita Supriadi Helny Rosita Supriadi Heni Pujiastuti HERA MAHESHWARI Heru Setijanto Heru Setijanto Heru Setijanto Heru Setijanto Huda Salahudin Darusman Huda Shalahudin Darusman I Ketut Mudite Adnyana ICHSAN EFFENDIE Idawati Nasution Idawati Nasution Iman Supriatna Ismahyuningsih, Revita Istiqmal, Kahfi Aulia Ita Djuwita Ita Djuwita Junzo Yamada Ketut Adnyane Mudite Kusdiantoro Mohamad Lidya Elizabeth M. Manik M Agus Setiadi M. Haviz M. Zairin Junior Masaji Washio Md Zuki Abu Bakar Mirnawati Sudarwanto Mokhamad Fahrudin Mokhamad Fakhrul Ulum, Mokhamad MOZES R. TOELIHERE MOZES TOELIHERE MUHAMMAD AGIL Muhammad Jalaluddin Muhammad Risman Wahid Mulyadi Adam MULYOTO PANGESTU Muslim Akmal Nabuo Kitamura Narindria, Yasmin Nadhiva Nastiti Kusumorini Nastiti Kusumorini Nazlie (Alm), Cutnya’ Shaliran Ni Luh Putu Rischa Phadmacanty Ni Wayan Kurniani Karja Nisa', Chairun Nisa’, Chairun Noordin Mohamed Mustapha Nuraini , Henny Nurhidayat Nurhidayat Nurhidayat Nurhidayat Nurhidayat Odilia Rovara Prawira, Andhika Yudha Putri Syifa Camilla R. Iis Arifiantini Rahma Anisa Razali . RIDWAN AFFANDI Riki Siswandi Ronny Rachman Noor Rusdin - Savitri Novelina Solly Aryza Sri Nurdiati Sri Wahyuni Sri Wahyuni Sriyanto - Supratikno Supratikno . Syahruddin Said TAKDIR SAILI Takdir Syahruddin Said Tongku Nizwan Siregar Trilaksono, Wahyu TUTI L YUSUF Tutik Wresdiyati Tuty L. Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf Ursula Paulawati Maker Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahyuni, Sri Wartika Rosa Farida Wartika Rosa Farida Yanri R.N. Simangunsong Yoshio Yamamoto Yuliani Suparmin