Article Per Year (5 Year)
p-Index From 2020 - 2025
7.955
P-Index
This Author published in this journals
All Journal IJCCS (Indonesian Journal of Computing and Cybernetics Systems) Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences JUSIFO : Jurnal Sistem Informasi JURNAL KEPEMIMPINAN DAN PENGURUSAN SEKOLAH Sistemasi: Jurnal Sistem Informasi Jurnal Ilmiah Ekonomi Islam AKSIOLOGIYA : Jurnal Pengabdian Kepada Masyarakat Jurnal Moral Kemasyarakatan INOVTEK Polbeng - Seri Informatika Jurnal Biologi Tropis INTECOMS: Journal of Information Technology and Computer Science Al Mi’yar: Jurnal Ilmiah Pembelajaran Bahasa Arab dan Kebahasaaraban Studi Arab Jurnal Ilmiah Ecosystem JIPI (Jurnal Ilmiah Penelitian dan Pembelajaran Informatika) JOISIE (Journal Of Information Systems And Informatics Engineering) EDUMATIC: Jurnal Pendidikan Informatika Building of Informatics, Technology and Science Jurnal Sistem informasi dan informatika (SIMIKA) Abdi Laksana : Jurnal Pengabdian Kepada Masyarakat Thariqah Ilmiah ; Jurnal ilmu-ilmu kependidikan & Bahasa Arab Jurnal Pengabdian kepada Masyarakat Nusantara ASANKA: Journal of Social Science And Education Jurnal Teknik Informatika (JUTIF) Al-tsaman : Jurnal Ekonomi dan Keuangan Islam Jurnal Pendidikan dan Teknologi Indonesia Jurnal Pendidikan PKn Journal Evaluation in Education (JEE) Jurnal Al-Fawa'id : Jurnal Agama dan Bahasa Jurnal Kapita Selekta Administrasi Publik Edu Sociata : Jurnal Pendidikan Sosiologi Jurnal Algoritma Maritime Park: Journal Of Maritime Technology and Socienty Aurelia: Jurnal Penelitian dan Pengabdian Masyarakat Indonesia Finger : Jurnal Ilmiah Teknologi Pendidikan De Cive : Jurnal Penelitian Pendidikan Pancasila dan Kewarganegaraan Jurnal Pembelajaran Bahasa dan Sastra Economics Studies and Banking Journal Rhizome: Jurnal Kajian Ilmu Humaniora Journal of Economic Growth and Development Review Society CARONG: Jurnal Pendidikan, Sosial dan Humaniora SCIENTIA JOURNAL : Jurnal Ilmiah Mahasiswa Journal of Supply Chain and Entrepreneurship EL-HAYAH
Syaifullah Syaifullah
Jurusan Sistem Informasi Fakultas Sains Dan Teknologi Universitas Islam Negeri Sultan Syarif Kasim Riau
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mechanical Engineering Social Sciences Other

Published : 69 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
DNA Barcoding and eDNA Metabarcoding for Identification Species: A Case Study (West Sumatra): DNA barcoding and eDNA metabarcoding Roesma, Dewi Imelda; Tjong, Djong Hon; Syaifullah, Syaifullah; Nofrita, Nofrita; Janra, Muhammad Nazri; Aidil, Dyta Rabbani; Prawira, Furqan Dwiki Lintang; Salis, Viola Mutiara
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/

Abstract

The biodiversity of freshwater fish is important to study because there is data and information that remain undiscovered. The waters of Sumatra, especially West Sumatra, are areas with high freshwater fish diversity but have limited information. Providing information and genetic data has become one of the important things to conduct. DNA barcoding and eDNA metabarcoding have become molecular methods for identifying species and providing information about the presence of species in a region. A study using DNA barcoding and eDNA metabarcoding was conducted on freshwater fish in several locations in West Sumatra. Isolation and amplification of DNA were performed directly on individual samples and sequenced using conventional methods (Sanger sequencing) to generate DNA barcodes. Water samples were collected (2 liters) at each location using a sterile bottle. The water samples were filtrated, isolated, and amplified using universal primer and sequenced with next-generation sequencing techniques. The study successfully collected 25 species belonging to 14 genera, 2 families, and 1 order. A total of 134 sequences from West Sumatra with a length of 648-670 bp were analyzed. All DNA barcodes were submitted to the BOLD System and GenBank, NCBI. The mean Kimura two-parameter model (K2P) genetic distances within species, genera, families, and orders were 0.7%, 8.3%, 15.8%, and 21.3%, respectively. The eDNA metabarcoding technique has successfully detected three native fish species in the waters of West Sumatra (Barbonymus schwanefeldii, Mystacoleucus padangensis, and Rasbora jacobsoni). The availability of fish DNA barcodes in reference databases is crucial for the success of identification using eDNA metabarcoding. Combining identification using conventional methods and eDNA metabarcoding can provide more reliable results and become a reference for future freshwater monitoring.
Co-Authors - Sapriya . Mansyurdin Abdul Azis Abdul Basit Abdur Rohman Adhari, Nursanda Rizki Adillah, Suci Nur Ahsyar, Tengku Khairil Aidil, Dyta Rabbani Anggyana, Rifa Angraini Angraini Anofrizen Anofrizen Aprillia, Dhea Dwi Aprillia, Dhea Dwi Aprinastya, Rachell Aqilla, Nabila Fadia Arif Marsal Arum Setiawan Asful, Ferdhinal Asmaret, Desi Asriyana, . Asyraf, Fajri Muhammad Aszareta, Muhammad Andoni Aurora, Dhea Apriano Ayulya, Agisti Mutiara Aziz, M. Abdul Cahyadi, Deden Cecep Darmawan, Cecep DADANG DADANG Dadang Sundawa, Dadang Dahelmi Dahelmi DAMIRAH DAMIRAH, DAMIRAH Dasim Budimansyah Dasim Budimansyah, Prayoga Bestari, Redi Yamanto, Dasim Budimansyah, Dewi Imelda Roesma Diani, Diani Djong Hon Tjong Efrizal Efrizal Eki Saputra Endang Danial, Endang Farisi, Czar Daffa Al Febi Nur Salisah Firdaus, Pipin Fitra, Joni Darma FITRIAH ARTINA, FITRIAH Fitriani Fitriani Guntara, Bima Hamzah, Muhammad Lutfhi Hamzah, Muhammad Lutfi Hamzah, Muhammad Luthfi Harmaniola, Elsa Hendra, Rio Hendri Irawan Henny Herwina Herliza, Herliza hidayanti, hidayanti Ida Waluyati Indah Lestari, Indah Indrajaya, Indrajaya Indriani, Monalisa Ingrid Shintia Arsy Jacky, Jacky Janra, Muhammad Nazri Jazman, Muhammad Juirah, Juirah Julhadi, Julhadi Julita, Sandra Junaihil, Junaihil Juniyati, Rita Juwandi, Ronni Karim Suryadi Karim Suryadi Kasno Kasno Kokom Komalasari Kosasih, Hengky Kyaw, Aung Ye Leni Anggraeni Lestari, Nurrizka Puji M Afdal Maliza, Rita Masyitoh, Iim Siti MAULANA, BIMA Maulidiyah, Khoirun Ni'mah Megawati - Mochamad Hasyim Mona Fronita, Mona Muarif, Muarif Muhammad Amin Muhammad Nanang Qosim Muslim Muslim Nanda Nazira Naufal, Misbahuddin Nazhifatunnisa, Nazhifatunnisa Ningsih, Widiah Nofrita Nofrita Noorahim, Wenny Noorahim Novita Sari Nugraha Nugraha Nurnazmi, Nurnazmi Nurrokhmatulloh, Nurrokhmatulloh Oktoriani, Della Pebri, Prianda Prananda, Yaldri Oktra Pratama, Raihan Anugrah Prawira, Furqan Dwiki Lintang Prima, Ari Putra Santoso Putra, Tarekh Febriana Putri, Runi Aulia Putri, Syntia Mai Rahman Mulyawan Rahmawita, Medyantiwi Ramadani, Ela Ramadhan, Rifky Ratna Fitria Rianda, Rahmad Didho Ridho, Muhammad Syifa'ur Safika, Nurul Sakban, Abdul Salis, Viola Mutiara Saputri, Aprilia Mayang Sianipar, Ferry Agus Sinaga, Lina Siregar, Syafril Solihah, Nikmatus Sri Nurhidayati, Sri Subakja, Triyawan Bagus Sulistiono Supriyono Supriyono Susanto Susanto Syahru Ramadan, Syahru Tasrif Tasrif Titik Efnita, Titik Tutoq, yulita Wahyuni, Reni wahyuni Waluyo Waluyo Wenny Noorahim Wilson Novarino Wily Julitawaty, Wily Yuniar, Sucifa Thita Zaini Zaini