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All Journal Media Kedokteran Hewan Jurnal Medik Veteriner Vitek : Bidang Kedokteran Hewan Jurnal Layanan Masyarakat (Journal of Public Service) Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary Narra J Livestock and Animal Research Buletin Pengabdian Journal of Fisheries & Marine Jurnal Agro Veteriner
Kadek Rachmawati
Division Of Basic Veterinary Faculty Of Veterinary Medicine Universitas Airlangga
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Education Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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PENGUJIAN VAKSIN HEPATITIS B FASE SUBKRONIS TERHADAP BERAT ORGAN DAN DIAMETER PULPA PUTIH LIMPA TIKUS PUTIH (Rattus norvegicus) Anindya, Afifatus; Santoso, Kuncoro Puguh; Rantam, Fedik Abdul; Rachmawati, Kadek; Nidom, Chairul Anwar; Widiyatno, Thomas Valentinus; Plumeriastuti, Hani
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20410

Abstract

This study aims to determine the effect of hepatitis B vaccine of spleen weight and white pulp diameter in rat (Rattus norvegicus). This study used 20 male wistar rats aged 8 weeks with an average weight of 200-250 grams, which was divided into four groups. Control group (P0) was given PBS solution, treatment group 1 (P1) was given type 1 hepatitis B vaccine, treatment group 2 was given type 2 hepatitis B vaccine, and treatment group 3 (P3) was given type 3 hepatitis B vaccine. Dose administered 0.4 ml and given repeatedly at days 0,30, and 60. Animals model to euthanize on day 75 for sampling. The spleen organ is weighed after the necropsing process and then the spleen is stored for the preparation of histopathologic with HE staining (Hematoxylin Eosin). The data obtained are weight data of spleen organ and spleen white pulp diameter. The results showed the highest weight of spleen organ 0.92 gram in group P0 and the largest white pulp diameter 34,34 μm in group P1. The results of data analyzed using one-way ANOVA (Analysis of Variance) and continued with Duncan alpha test. One Way Anova and Duncan alpha test showed that there were non significantly different between groups (p>0.05). The conclusion is we know that hepatitis B vaccine type 1,2, and 3 can't give the effect for spleen weight and white pulp diameter in rat (Rattus norvegicus).
HISTOPATHOLOGICAL CHANGES OF KIDNEY OF BROILER CHICKEN EXPOSED TO CHRONIC HEAT STRESS Xin, Irene Teh Kai; Plumeriastuti, Hani; Anwar, Chairul; Rachmawati, Kadek; Utama, Suzanita; Legowo, Djoko
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20411

Abstract

The aim of this research was to know histopathological changes of kidney of broiler chicken exposed to chronic heat stress. Twenty broilers were divided randomly into 2 groups, 10 broilers each are being exposed to chronic heat stress for 21 days. The first 21 days were the adaptation period in a chamber with temperature 24-28o C and humidity 40-55%. After 21 days, continue with exposure to heat stress in a chamber with temperature 36-40o C and humidity 50-65% in 8 hours per day. After exposed to chronic heat stress, kidney tissues were processed, and kidney tissue histopathological changes were evaluated by using the Klopfleisch modified scoring method. The data was analyzed by Mann – Whitney Test. The result of this research showed that chronic heat stress exposure causing the presence of degeneration of tubular epithelial cell, necrosis of tubular epithelial cell, necrosis of glomerular and interstitial infiltration. From the analysis data the overviewof multiparametric showed that when control group compared with the treatment group showed significantly difference (p<0.05). 
The Anthelmintic Activity Of Etanol Extract of Porang Tubers Against Fasciola gigantica In Vitro Agustina, Firanda; Kurnijasanti, Rochmah; Suwanti, Lucia Tri; Lastuti, Nunuk Dyah Retno; Rachmawati, Kadek; Maslachah, Lilik
Journal of Basic Medical Veterinary Vol. 10 No. 2 (2021): Journal of Basic Medical Veterinary, December 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i2.31235

Abstract

This study aims to determine the anthelmintic activity ethanol extract of porang tubers (A. oncophillus) against F. gigantica worms in vitro. There were five treatments and each treatment was done in five replications and used 10 F. gigantica. The treatments of this research were K- with CMC Na 1%, K+ with Albendazole 2.4 mg/ml, P1 with extract concentration 5%, P2 with extract concentration 10%, P3 with extract concentration 20%. The results showed that the extract of Porang tuber (A. oncophillus) had an anthelmintic effect against F. gigantica worms in vitro. In the extract with a concentration of 20%, there were anthelmintic properties that almost the same as Albendazole. The higher the extract concentration, the higher the anthelmintic properties. The longer the immersion time, the higher the number of dead worms. The morphological changes was evaluated by light microscopic examination and the results showed many histopathological changes on the morphology of F. gigantica. The results indicate that the possible use of the tubers as a potential anthelmintic against F. gigantica.
The Effect of Mimosa Pudica Root Extract on Cerebrum Histopathological of Rattus Norvegicus Induced with Naja Sputatrix Venom Permatasari, Roselia Yuliani; Hestianah, Eka Pramyrtha; Legowo, Djoko; Rachmawati, Kadek; Arifin, Zainal
Journal of Basic Medical Veterinary Vol. 11 No. 1 (2022): Journal of Basic Medical Veterinary, June 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v11i1.36485

Abstract

The aim of this study was to know the effect of Mimosa pudica root extract on histopathological appearance of Rattus norvegicus brain induced by Naja sputatrix venom. Thirty rats were divided into 5 groups. There were 2 control groups and 3 treatment groups, which was given 250, 500, and 1000 mg/kg BW of Mimosa pudica root extract orally. The first 7 days each group was adapted to the environment. On the 8th day, the treatment was started by injecting Naja sputatrix LD50 (0,13 L/gram BW) IM in gluteus muscle, continued with giving Mimosa pudica root extract orally for the treatment groups 5 minutes after venom injection. 6 hours after the last treatment, rats were killed by cervical dislocation, injected with formalin 10% in the heart, then necropsied. Histopathological evaluation was done to score brain damage based on meningitis, perivascular cuffing, and necrotic cells using HE stain with 1000x magnification. The result showed 1000 mg/kg BW dosage of Mimosa pudica root extract can reduce brain damage based on meningitis, perivascular cuffing, and necrotic cells in Rat (Rattus norvegicus) caused by Naja sputatrix venom and gave significant difference (p < 0.05) among the treatment groups.
Screening of Blood Glucose Concentration in Domestic Cat (Felis Catus) Based on Body Condition Score, Breed, and Sex Using Portable Blood Glucose Meter Billa, Lutfiah Annisa; Triakoso, Nusdianto; Rachmawati, Kadek; Yuniarti, Wiwik Misaco; Aksono, Eduardus Bimo; Yudaniayanti, Ira Sari
Journal of Basic Medical Veterinary Vol. 12 No. 1 (2023): Journal of Basic Medical Veterinary, June 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i1.42246

Abstract

Type 2 diabetes mellitus (T2DM) is a common endocrine disease in domestic cats. Breed, sex, being overweight to an extent of obesity are predisposing factors for developing T2DM. One of the most common laboratory analyses conducted to confirm this disease is blood glucose concentrations. The aim of this research is to determine blood glucose concentration in domestic cats based on BCS, breed, and sex using a portable blood glucose meter. Research samples were obtained by taking blood samples from the marginal ear vein of 131 domestic cats that matched the sample's criteria, took at 2-hours post prandial, and drip onto the Sinocare strip. The results showed that the average blood glucose concentration was in the normal range, with a total of ideal (BCS ≥3/5) domestic cats (n=43) was 75.74 mg/dL, overweight (BCS ≥4/5) domestic cats (n=44) was 78.23 mg/dL, obese (BCS ≥5/5) domestic cats (n=44) was 89.66 mg/dL, male (n=51) was 80.57 mg/dL, female (n=80) was 81.69 mg/dL, Mixed Breed (n= 119) was 81.03 mg/dL, Angora (n=5) was 84.20 mg/dL, Persian (n=6) was 81.50 mg/dL and Ragdoll (n=1) was 92 mg/dL. Pearson correlation showed BCS (r=0.403; p= 0.000) had a moderate, positive, and significant correlation with blood glucose concentration in domestic cats. Meanwhile, the results of Kendall's Tau correlation showed that breed (p=0.740) and sex (p=0.555) had insignificant correlation with blood glucose concentration in domestic cats.
The Effectiveness of Ethanol Extract of Ketapang Leaves (Terminalia catappa L.) as a Larvicidal Against Aedes aegypti Mosquito Larvae Dwi Ananda, Novita; Rachmawati, Kadek; Lastuti, Nunuk Dyah Retno; Suwanti, Lucia Tri; Hidajanti, Nove; Meles, Dewa Ketut
Journal of Basic Medical Veterinary Vol. 12 No. 1 (2023): Journal of Basic Medical Veterinary, June 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i1.42248

Abstract

This study aimed to determine the effectiveness of the ethanol extract of ketapang leaves (Terminalia catappa) on mortality and histopathology of Ae. aegypti instar III as an effort to control Ae. aegypti as a disease vector without causing resistance to the larvae. The extraction process uses the maceration method. This type of research is a laboratory experiment with research subjects Ae. aegypti instar III of 700 individuals, which were divided into 7 treatment groups (1 negative control group using distilled water and 1% DMSO, 1 positive control group using 1000 ppm abate, ethanol extract of ketapang leaves with concentrations of 2000 ppm, 2500 ppm, 3500 ppm, 5000 ppm, 7000 ppm) and 4 repetitions. The results of this study indicate that the negative control (K-) mortality rate is 0% and the positive control (K+) mortality percentage is 100%. The results of the ethanol extract of ketapang leaves (Terminalia catappa) on the mortality of Ae. aegypti instar III in treatment one (P1) the percentage of mortality was 17%, treatment two (P2) the percentage of mortality was 21%, treatment three (P3) the percentage of mortality was 23%, treatment four (P4) the percentage of mortality was 27%, and treatment five (P5) the percentage of mortality is 42%. Histopathological observations on larvae that died after being exposed to ethanol extract of ketapang leaves (Terminalia catappa) for 24 hours damaged the basement membrane, peritropic membrane and epithelial cells. It can be concluded that the ethanol extract of ketapang leaves (Terminalia catappa) is effective as a larvicidal against Ae. aegypti instar III but can cause damage to the midgut and cause death of the larvae.
The Effect of Giving Gel Combination of Binahong Leaf Extract and Turmeric Rhizome Extract on Histopathological Epithelial Thickness in II B Degree Burn of Rattus norvegicus Indriyanti, Astrid; Rachmawati, Kadek; Maslachah, Lilik; Damayanti, Ratna; Hidayatik, Nanik; Setiawan, Boedi
Journal of Basic Medical Veterinary Vol. 12 No. 2 (2023): Journal of Basic Medical Veterinary, December 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i2.45753

Abstract

This aim of this research was to study the effect of giving a combination gel of binahong (Anredera cordifolia) leaf and turmeric (Curcuma longa Linn) rhizome extract on histopathology of epithelial thickness which has II B degree burns. Twenty five male white rats (Rattus norvegicus) were divided into five groups. K(-) group was normal skin, K(+) group was skin burns treated with 1% silver sulfadiazine, P1, P2, and P3 was skin burns treated with gel combination of binahong leaf extract and turmeric rhizome extract with increase concentration of binahong leaf extract i.e. 1.25%, 2.5%, and 5%, while the concentration of turmeric rhizome extract i.e. 2% for each treatment. The combination gel of binahong leaf extrac 1.25% and turmeric rhizome extract 2% has potential to be an effective treatment for II B burns as indicated by increased of epithelial thickness. Therefore, this study concluded that 1.25% combination gel of binahong leaf extract and turmeric rhizome extract 2% is the effective dosage to increase epithelial thickness on II B healing degree burns wound healing.
Antibacterial Activity of Bidara Leaf Extract (Ziziphus mauritiana) Against Staphylococcus aureus Isolated from Mastitis Case In Vitro Saraswati, Afif Tasya; Sugihartuti, Rahmi; Puspitasari, Yulianna; Witaningrum, Adiana Mutamsari; Rachmawati, Kadek; Maslachah, Lilik; Raharjo, Hartanto Mulyo; Hisyam, Mirza Atikah Madarina; Tacharina, Martia Rani
Journal of Basic Medical Veterinary Vol. 12 No. 2 (2023): Journal of Basic Medical Veterinary, December 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i2.51106

Abstract

This research aimed to know the antibacterial activities of bidara leaf extract against Staphylococcus aureus isolated from mastitis cases. The isolate was identified before the antibacterial test. This study was done by five times repetitions on Mueller Hinton Agar. The concentrations used in this study were 30%, 40%, and 50% of bidara leaf extract then a blank disc was dipped into each concentration. Tetracycline disc was used as a positive control and aquadest was used as a negative control. The diameter of the clear zone was measured using calipers after incubation for 24 hours at 37℃. The results showed significant differences (p<0.05) between the positive control (K+), negative control K (-), and the concentration group. However, each concentration of bidara leaf extract showed insignificant differences in the diameter of the clear zone. It could be concluded that bidara leaf extract has antibacterial activities against Staphylococcus aureus isolated from mastitis cases.
The Effect of Red Ginger Extract (Zingiber officinale var rubrum) on The Thickness of The Epitelial and Diameter of Seminiferous Tubules in Albino Rat (Rattus norvegicus) Exposed Monosodium Glutamat Renaldi, Nogi; Rachmawati, Kadek; Widiyatno, Thomas Valentinus; Madyawati, Sri Pantja; Dhamayanti, Yeni
Journal of Basic Medical Veterinary Vol. 13 No. 1 (2024): Journal of Basic Medical Veterinary, June 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v13i1.51158

Abstract

Background: This study examined administration of MSG intraperitoneally to white rats resulted in a decrease in testicular weight, an increase in lipid peroxidase levels The testes, as the site of spermatogenesis, are highly vulnerable to oxidation processes caused by free  radicals,  which  can  disrupt spermatogenesis. Free radicals can be countered by antioxidants. One plant believed to have antioxidant activity is red ginger. Purpose: To determine the effect of red ginger against histopathology seminiferous tubules of white rat (Rattus norvegicus) exposed by monosodium glutamate. Methods: This study used 30 rats which were divided into 5 groups. The positve control group (K+) were given only monosodium glutamate 5g/kgBW and CMC Na orally, the treatments groups P1 were given monosodium glutamte 5g/KgBW + red ginger 50mg/rat/day, P2 were given 5g/KgBW + red ginger 100mg/rat/day, and P3 were given 5g/KgBW + red ginger 200mg/rat/day. All treatments were administered for 42 days. After 42 days of treatments, all rats were sacrificed and seminiferous tubules were observed with microscope from pathology laboratory. Results: Administration of red ginger extract can defend seminiferous tubules epithelial thicknes and diameter from exposure of monosodium glutamate with 100mg/KgBW dosage as the most effective dosage. Seminiferous tubules epitelial thickness and diameter were tested using one-way ANOVA and Duncan's multiple range test. Conclusion: Red Ginger Extract (Zingiber officinale var rubrum) has shown potential effects to counteract Monosodium Glutamat consequences on The Thickness of the epitelial and diameter of seminiferous tubules of white rat (Rattus norvegicus).
The Effects of Prebiotic Mannan Oligosaccharide (MOS) Supplementation on Hematology Profile of Native Chickens (Gallus gallus domesticus) Dewi, Fullan Ausati Putri; Utami, Prima Sukma Rizky; Rusyda, Tsamara Aulia; Kusuma, Dhio Salhisya Marta; Maharani, Disty Putri; Bima, Dewangga Arya; Rachmawati, Kadek
Journal of Basic Medical Veterinary Vol. 14 No. 1 (2025): Journal of Basic Medical Veterinary, June 2025
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v14i1.66539

Abstract

Native chickens (Gallus gallus domesticus) are an important source of animal protein due to their adaptability and relatively simple management. However, their productivity remains limited, which poses a significant challenge in poultry farming. One promising strategy to improve the health and productivity of native chickens is supplementation with Mannan Oligosaccharides (MOS), a prebiotic known to enhance gut microflora balance, strengthen immune responses, and support overall health. This study aimed to evaluate the impact of MOS supplementation on the hematological profile of native chickens. A true experimental method with a control group pre-test post-test design was employed. The results demonstrated that MOS supplementation significantly improved the hematological profile of native chickens. Notable increases were observed in the red blood cell (RBC) count to 2.54 × 10⁶ cells/mm³, white blood cell (WBC) count to 20.30 × 10³ cells/mm³, hemoglobin concentration to 11.4 g/dL, and hematocrit value to 31%. These findings highlight the potential of MOS supplementation to enhance the health and productivity of Native chickens through improvements in key hematological parameters.
Co-Authors . Kholik Adiana Mutamsari Witaningrum Agustina, Firanda Aksono HP., Eduardus Bimo Anindya, Afifatus Ansori, Arif NM. Ardhanella, Sellianova Atmaja, Peter Y. Bambang Sektiari Lukiswanto Billa, Lutfiah Annisa Bima, Dewangga Arya Boedi Setiawan Bura, Maria Antonia Yersi Dua Chairul Anwar Dadik Rahardjo, Dadik Derkho, Marina Dewi, Fullan Ausati Putri Durry, Fara D. Dwi Ananda, Novita Eka Pramyrtha Hestianah, Eka Pramyrtha Erma Safitri Ethica, Stalis N. Fedik Abdul Rantam Hamzah, Arshq Mirza Hani Plumeriastuti Hidajanti, Nove Hidanah, Sri Hidayatik, Nanik Hisyam, Mirza Atikah Madarina Humairah, Ira IMAM MUSTOFA Imam Mustofa Indriyanti, Astrid Ira Sari Yudaniayanti Iva Nur Silviana Iwan Sahrial Hamid Jamaluddin, Indra P. Khairullah, Aswin Rafif Kusuma, Dhio Salhisya Marta Larasati, Anissa Dhea Legowo, Djoko Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Lukitasari, Lina Maharani, Disty Putri Meles, Dewa Ketut Mirino, Mario Navyseal Mirni Lamid Muhammad Hidayat Murtadlo, Ahmad AA. Musa, Susan H. Mustofa Helmi Effendi Naw, Sin W. Nidom, Chairul Anwar Nunuk Dyah Retno Lastuti Nurul Azizah Nusdianto Triakoso Permatasari, Roselia Yuliani Prabowo, Gwenny Ichsan Pradana, Munawer Putri, Desak Ketut Sekar Cempaka Qurnianingsih, Ema Rahadian Zainul Raharjo, Hartanto Mulyo Rahmahani, Jola Rahmatullah, Aldin Akbar Rahmi Sugihartuti, Rahmi Ratna Damayanti Rebezov, Maksim Renaldi, Nogi Ristaqul Husna Belgania Riwu, Katty Hendriana Priscilia Rochmah Kurnijasanti Rusyda, Aqila sabila Rusyda, Tsamara Aulia S Ravi, Vishantheni Sabdoningrum, Emy Koestanti Santoso, Kuncoro P. Santoso, Kuncoro Puguh Saraswati, Afif Tasya Siti Khaerunnisa Soeharsono Soeharsono Soeharsono Sri Mulyati Sri Pantja Madyawati, Sri Pantja Suherni Susilowati Sukma Sahadewa, Sukma Sukmanadi, M Suryaningtyas, Ignasia Friska Amelia Suwarno Suwarno Suwasanti, Ni Luh Suzanita Utama Tacharina, Martia Rani Tri Wahyu Suprayogi Tyasningsih, Wiwiek Ummah, Fithriyah Cholifatul Ummah Utami, Prima Sukma Rizky Widiyatno, Thomas Valentinus Widya Paramita Lokapirnasari Wiwik Misaco Yuniarti Wungu, Citrawati Dyah Kencono Wurlina, W Xin, Irene Teh Kai Yeni Dhamayanti Yosephi, Valensa Yulianna Puspitasari Zainal Arifin