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All Journal Media Kedokteran Hewan Jurnal Medik Veteriner Vitek : Bidang Kedokteran Hewan Jurnal Layanan Masyarakat (Journal of Public Service) Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary Narra J Livestock and Animal Research Buletin Pengabdian Journal of Fisheries & Marine Jurnal Agro Veteriner
Kadek Rachmawati
Division Of Basic Veterinary Faculty Of Veterinary Medicine Universitas Airlangga
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Education Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Journal : Narra J

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Detection of Pseudomonas aeruginosa pus wound isolate using a polymerase chain reaction targeting 16S rRNA and gyrB genes: A case from Indonesia Jamaluddin, Indra P.; Musa, Susan H.; Ethica, Stalis N.; Ansori, Arif NM.; Yosephi, Valensa; Atmaja, Peter Y.; Murtadlo, Ahmad AA.; Sahadewa, Sukma; Durry, Fara D.; Rebezov, Maksim; Derkho, Marina; Naw, Sin W.; Zainul, Rahadian; Rachmawati, Kadek
Narra J Vol. 4 No. 2 (2024): August 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i2.774

Abstract

Infectious wounds on the skin surface are easily colonized by bacteria from pyogenic group that manifest as inflammation, such as Pseudomonas aeruginosa. P. aeruginosa is a Gram-negative bacterium and an opportunistic pathogen known for causing invasive state in critically ill and immunocompromised patients. The aim of this study was to detect the 16S rRNA and gyrB genes in P. aeruginosa using polymerase chain reaction (PCR) method. The sample in this study was pus isolate from a 5-year-old boy with leg wounds. The bacteria were isolated on brain heart infusion broth (BHIB) media and identified with molecular identification. Sequencing and BLAST analysis were carried out to determine the similarity of gene identity by comparing sample sequence with other isolate sequences on the Gene Bank. The results of molecular identification showed amplification DNA band of around 934 base pairs (bp) for 16S rRNA and 225 bp for gyrB gene. The BLAST program demonstrated that the sample had 99.89% similarity with P. aeruginosa strain XC4 (accession code ON795960.1) for the 16S rRNA gene. Meanwhile, the gyrB gene exhibited 99.10% similarity with the P. aeruginosa strain PSA-1.2 (accession code KP172300.1).
Co-Authors . Kholik Adiana Mutamsari Witaningrum Agustina, Firanda Aksono HP., Eduardus Bimo Anindya, Afifatus Ansori, Arif NM. Ardhanella, Sellianova Atmaja, Peter Y. Bambang Sektiari Lukiswanto Billa, Lutfiah Annisa Bima, Dewangga Arya Boedi Setiawan Bura, Maria Antonia Yersi Dua Chairul Anwar Dadik Rahardjo, Dadik Derkho, Marina Dewi, Fullan Ausati Putri Durry, Fara D. Dwi Ananda, Novita Eka Pramyrtha Hestianah, Eka Pramyrtha Erma Safitri Ethica, Stalis N. Fedik Abdul Rantam Hamzah, Arshq Mirza Hani Plumeriastuti Hidajanti, Nove Hidanah, Sri Hidayatik, Nanik Hisyam, Mirza Atikah Madarina Humairah, Ira IMAM MUSTOFA Imam Mustofa Indriyanti, Astrid Ira Sari Yudaniayanti Iva Nur Silviana Iwan Sahrial Hamid Jamaluddin, Indra P. Khairullah, Aswin Rafif Kusuma, Dhio Salhisya Marta Larasati, Anissa Dhea Legowo, Djoko Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Lukitasari, Lina Maharani, Disty Putri Meles, Dewa Ketut Mirino, Mario Navyseal Mirni Lamid Muhammad Hidayat Murtadlo, Ahmad AA. Musa, Susan H. Mustofa Helmi Effendi Naw, Sin W. Nidom, Chairul Anwar Nunuk Dyah Retno Lastuti Nurul Azizah Nusdianto Triakoso Permatasari, Roselia Yuliani Prabowo, Gwenny Ichsan Pradana, Munawer Putri, Desak Ketut Sekar Cempaka Qurnianingsih, Ema Rahadian Zainul Raharjo, Hartanto Mulyo Rahmahani, Jola Rahmatullah, Aldin Akbar Rahmi Sugihartuti, Rahmi Ratna Damayanti Rebezov, Maksim Renaldi, Nogi Ristaqul Husna Belgania Riwu, Katty Hendriana Priscilia Rochmah Kurnijasanti Rusyda, Aqila sabila Rusyda, Tsamara Aulia S Ravi, Vishantheni Sabdoningrum, Emy Koestanti Santoso, Kuncoro P. Santoso, Kuncoro Puguh Saraswati, Afif Tasya Siti Khaerunnisa Soeharsono Soeharsono Soeharsono Sri Mulyati Sri Pantja Madyawati, Sri Pantja Suherni Susilowati Sukma Sahadewa, Sukma Sukmanadi, M Suryaningtyas, Ignasia Friska Amelia Suwarno Suwarno Suwasanti, Ni Luh Suzanita Utama Tacharina, Martia Rani Tri Wahyu Suprayogi Tyasningsih, Wiwiek Ummah, Fithriyah Cholifatul Ummah Utami, Prima Sukma Rizky Widiyatno, Thomas Valentinus Widya Paramita Lokapirnasari Wiwik Misaco Yuniarti Wungu, Citrawati Dyah Kencono Wurlina, W Xin, Irene Teh Kai Yeni Dhamayanti Yosephi, Valensa Yulianna Puspitasari Zainal Arifin