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All Journal Jurnal Kedokteran Brawijaya Majalah Kedokteran Bandung Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Universa Medicina AKSIOLOGIYA : Jurnal Pengabdian Kepada Masyarakat Jurnal Medik Veteriner Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Parasite Science Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary Journal of Pharmaceutical and Sciences. Journal of Fisheries & Marine Jurnal Agro Veteriner
Lilik Maslachah
Department Of Veterinary Basic Medicine, Faculty Of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, 60115. Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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PENETAPAN RETENTION TIME PROGESTERON DALAM PELARUT ELUEN PHASE MOBILE MENGGUNAKAN HIGH PERFORMANCE LIQUID CHROMATOGRAPHY Chandra, Evania Haris; Lazuardi, Mochamad; Sarudji, Suryanie; Maslachah, Lilik; Sukmanadi, Mohammad; Utama, Suzanita
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21090

Abstract

The aim of this research was to determine the level of stability of Progesterone-retention time on Eluen Phase Mobile (EPM) solution in storage period for six, eight and 12 hours using High Performance Liquid Chromatography (HPLC). The research method used posttest-only control group design by using three treatments and six repetitions. The three repetitions consist into six hours, eight hours and 12 hours. The data were obtained analyzed by Summery Independent T-Test with SPSS 24 for windows. The results showed that the progesterone treatment in EPM solvents with six-hour storage (P1) and eight hours (P2) retention time progesterone are stable but the progesterone treatment in EPM solvent with 12-hour storage (P3) showed an unstable due to storage time. Besides that there are irrelevant peak which show that many pure molecular fractions are separated in the stationary phase so that the molecular structure of the substance begins to break down. Based on these results, it can be concluded that the storage time of Progesterone in Eluen Phase Mobile began to be unstable at 12 hours of storage. 
EFEKTIVITAS PEMBERIAN NANOPARTIKEL EKSTRAK DAUN JUWET (Syzygium cumini) SEBAGAI ADJUVANT TERAPI TERHADAP GAMBARANHISTOPATOLOGI PULMO MENCIT (Mus musculus) YANG DIINFEKSI Plasmodium bhergei Ayu, Zerlinda Dyah; Maslachah, Lilik; Supranianondo, Koesnoto; Suprihati, Endang; Plumeriastuti, Hani; Sugihartuti, Rahmi
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21091

Abstract

The purpose of this study was to learn how to use juwet nanoparticle leaf extract (Syzygium cumini) as an adjunct therapy in pulmonary histopathological mice (Mus musculus) supported by Plasmodium berghei. This study used 30 rats divided into six groups consisting of K0 (disagree without agreement), P1 (fight without agreement), P2 (fight and dispute with chloroquine), P3 (fight and talk with juwet leaf extract), P4 (useful and useful with juwet leaf extract)) and P5 (extracts and combinations given with chloropine and juwet leaf extract nanoparticles) protected by 1x105 in 0.2 ml of Plasmodium berghei. Data were analyzed by Kruskal Wallis using SPSS and continued with the Mann-Whitney test. The results of this study showed that there were significant differences (p <0.05) between P2 and P5, but did not show a significant difference (p> 0.05) with P1, P3 and P4. Based on this study, can be witnessed by juwet nanoparticles (Syzygium Cumini) additional therapeutic leaf extract which is expected to reduce pulmonary damage (hemmoragi, alveolar congestion, edema, hemosiderin) mice (Mus musculus) infected with Plasmodium berghei. 
UJI PENETAPAN STABILITAS RETENTION TIME Megestrole acetate DALAM ELUENT MOBILE PHASE MENGGUNAKAN HIGH PERFORMANCE LIQUID CHROMATOGRAPHY Cholifah, Siti Chusnul; Lazuardi, Mochamad; Rahardjo, Dadik; Maslachah, Lilik; Sukmanadi, Mohammad; Kurnijasanti, Rochmah
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21093

Abstract

The aim of this research was to determine the level of stability of Megestrole acetate-retention time in storage period for six, eight and 12 hours using High Performance Liquid Chromatography (HPLC). The research method used posttest-only control group design by using three treatments and six repetitions. The three repetitions consist into six hours, eight hours and 12 hours. The data were obtained analyzed by Summery Independent T-Test with SPSS 24 for windows. The result showed six hours retention time of Megestrole acetate is stable and eight hours treatment and 12 hours treatment are not stable there is one unstable point of 12 hours treatment that indicates the substance is break down. Based on those result, it could be concluded that the storage time of Megestrole acetate in Eluent Mobile Phase began to show unstable at eight hours of storage.
KEMAMPUAN MALARISIDAL NANOPARTIKEL EKSTRAK DAUN JUWET (Syzygium cumini) SEBAGAI TERAPI AJUVAN PADA HISTOPATOLOGI HATI MENCIT (Mus musculus) PENDERITA MALARIA Pujiyanto, Mahendra; Maslachah, Lilik; Triakoso, Nusdianto; Lazuardi, Mochamad; Anwar, Chairul; Legowo, Djoko
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21094

Abstract

The aimed of this study is to know malaricidal ability of nanoparticle juwet (Syzygium cumini) leaf extract as adjuvant therapy on liver histopathological of mice (Mus musculus) which infected by Plasmodium berghei. This research uses 30 mice which are divided into six treatment groups namely K0 (not infected without treated), K+ (infected without treated), K1 (infected and treated with chloroquin), K2 (infected and treated with juwet leaf extract), P1 (infected and treated with juwet leaf extract nanoparticle) and P2 (infected and combination treated with chloroquine and juwet leaf extract nanoparticle) which infected by 1x105 in 0.2 ml of Plasmodium berghei. The data were analyzed by Kruskal Wallis using SPSS and followed with Mann-Whitney test. This research result showed there was significant difference (p < 0.05) between K1 and P2, but did not showed significant difference (p > 0.05) with K+, K2 and P1. Based on this research, it could be concluded that nanoparticle juwet (Syzygium cumini) leaf extract as adjuvant therapy estimated can increase malaricidal ability in degeneration, necrosis, portal inflammation, Kupffer cell hyperplasia, sinusoidal congestion, and hemosiderosis in mice liver which infected by Plasmodium berghei.
The Anthelmintic Activity Of Etanol Extract of Porang Tubers Against Fasciola gigantica In Vitro Agustina, Firanda; Kurnijasanti, Rochmah; Suwanti, Lucia Tri; Lastuti, Nunuk Dyah Retno; Rachmawati, Kadek; Maslachah, Lilik
Journal of Basic Medical Veterinary Vol. 10 No. 2 (2021): Journal of Basic Medical Veterinary, December 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i2.31235

Abstract

This study aims to determine the anthelmintic activity ethanol extract of porang tubers (A. oncophillus) against F. gigantica worms in vitro. There were five treatments and each treatment was done in five replications and used 10 F. gigantica. The treatments of this research were K- with CMC Na 1%, K+ with Albendazole 2.4 mg/ml, P1 with extract concentration 5%, P2 with extract concentration 10%, P3 with extract concentration 20%. The results showed that the extract of Porang tuber (A. oncophillus) had an anthelmintic effect against F. gigantica worms in vitro. In the extract with a concentration of 20%, there were anthelmintic properties that almost the same as Albendazole. The higher the extract concentration, the higher the anthelmintic properties. The longer the immersion time, the higher the number of dead worms. The morphological changes was evaluated by light microscopic examination and the results showed many histopathological changes on the morphology of F. gigantica. The results indicate that the possible use of the tubers as a potential anthelmintic against F. gigantica.
The Effect of Giving Gel Combination of Binahong Leaf Extract and Turmeric Rhizome Extract on Histopathological Epithelial Thickness in II B Degree Burn of Rattus norvegicus Indriyanti, Astrid; Rachmawati, Kadek; Maslachah, Lilik; Damayanti, Ratna; Hidayatik, Nanik; Setiawan, Boedi
Journal of Basic Medical Veterinary Vol. 12 No. 2 (2023): Journal of Basic Medical Veterinary, December 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i2.45753

Abstract

This aim of this research was to study the effect of giving a combination gel of binahong (Anredera cordifolia) leaf and turmeric (Curcuma longa Linn) rhizome extract on histopathology of epithelial thickness which has II B degree burns. Twenty five male white rats (Rattus norvegicus) were divided into five groups. K(-) group was normal skin, K(+) group was skin burns treated with 1% silver sulfadiazine, P1, P2, and P3 was skin burns treated with gel combination of binahong leaf extract and turmeric rhizome extract with increase concentration of binahong leaf extract i.e. 1.25%, 2.5%, and 5%, while the concentration of turmeric rhizome extract i.e. 2% for each treatment. The combination gel of binahong leaf extrac 1.25% and turmeric rhizome extract 2% has potential to be an effective treatment for II B burns as indicated by increased of epithelial thickness. Therefore, this study concluded that 1.25% combination gel of binahong leaf extract and turmeric rhizome extract 2% is the effective dosage to increase epithelial thickness on II B healing degree burns wound healing.
Antibacterial Activity of Bidara Leaf Extract (Ziziphus mauritiana) Against Staphylococcus aureus Isolated from Mastitis Case In Vitro Saraswati, Afif Tasya; Sugihartuti, Rahmi; Puspitasari, Yulianna; Witaningrum, Adiana Mutamsari; Rachmawati, Kadek; Maslachah, Lilik; Raharjo, Hartanto Mulyo; Hisyam, Mirza Atikah Madarina; Tacharina, Martia Rani
Journal of Basic Medical Veterinary Vol. 12 No. 2 (2023): Journal of Basic Medical Veterinary, December 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i2.51106

Abstract

This research aimed to know the antibacterial activities of bidara leaf extract against Staphylococcus aureus isolated from mastitis cases. The isolate was identified before the antibacterial test. This study was done by five times repetitions on Mueller Hinton Agar. The concentrations used in this study were 30%, 40%, and 50% of bidara leaf extract then a blank disc was dipped into each concentration. Tetracycline disc was used as a positive control and aquadest was used as a negative control. The diameter of the clear zone was measured using calipers after incubation for 24 hours at 37℃. The results showed significant differences (p<0.05) between the positive control (K+), negative control K (-), and the concentration group. However, each concentration of bidara leaf extract showed insignificant differences in the diameter of the clear zone. It could be concluded that bidara leaf extract has antibacterial activities against Staphylococcus aureus isolated from mastitis cases.
Isolation and Identification of Bacterial Biosurfactant Activity from Mangrove Sediments Sugihartuti, Rahmi; Pradana, Munawer; Maslachah, Lilik; Chusniati, Sri; Tyaningsih, Wiwiek; Rahardjo, Dadik; Soeharsono, Soeharsono
Journal of Basic Medical Veterinary Vol. 13 No. 1 (2024): Journal of Basic Medical Veterinary, June 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v13i1.58266

Abstract

Background: Ujung Pangkah mangroves are reported to have been contaminated with heavy metals. Such heavy metals can induce microorganisms to produce biosurfactants. Biosurfactants with amphiphilic characteristics can lower surface tension. Biosurfactants can be used as antibacterial, antifungal, and antiviral for biomedical purposes. Purpose: This study aimed to identify and test the activity of biosurfactant isolates of bacteria from Ujung Pangkah Mangrove sediments, Gresik Regency. Methods: Biosurfactant activity test methods include emulsification index, oil spreading, drop collapse, and parafilm test. Results: The results of the identification of bacterial isolates in this study obtained the genus Bacillus sp. because bacterial isolates show rod shape, Gram-positive, aerobic, and have ellipse-shaped endospores on the subterminal. The results of the biosurfactant activity test with the Emulsification index method showed an average result of 54.39% and the results of the biosurfactant activity test with the oil spreading method showed that there was a clear zone. The average result of the clear zone obtained is 54.83 mm. The average result in the parafilm test was 8.02 mm and the drop collapse test showed positive results characterized by falling and spreading of bacterial isolate fermentation broth supernatants.
Pemberdayaan Kelompok Asuhan Mandiri Manggala melalui Pemanfataan Tanaman Toga dengan Pupuk Organik Koestanti S, Emy; Suprihati , Endang; Sukartini, Tintin; Hidanah, Sri; Paramita Lokapirnasari, Widya; Susilowati, Suherni; Maslachah, Lilik; Angelina Hendarti, Gracia
Aksiologiya: Jurnal Pengabdian Kepada Masyarakat Vol 8 No 4 (2024): November
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/aks.v8i4.24107

Abstract

Tanaman Obat Keluarga (TOGA) merupakan jenis tanaman yang memiliki dampak positif secara farmakologis pada tubuh manusia dan umumnya ditanam baik di lingkungan rumah tangga maupun dalam skala komunal. Penelitian ilmiah terhadap tanaman obat keluarga yang memiliki khasiat pengobatan juga mengungkapkan bahwa tanaman tersebut mengandung zat-zat atau senyawa aktif yang terbukti memberikan manfaat bagi kesehatan. Fokus pengabdian masyarakat, meningkatkan pengetahuan dan ketrampilan pada kelompok asuhan mandiri tentang aplikasi pupuk organik sebagai media untuk Taman Toga yang ramah lingkungan dan aplikasi pembuatan jejamuan metode ekstraksi untuk mengeluarkan zat aktif pada tanaman herbal. Hasil evaluasi menunjukkan 100% peserta menanam temulawak, sebanyak 35 peserta (81%) menanam temulawak dan kunir sebanyak 25 peserta menanam temulawak, kunir dan sambiloto (58%). Pengetahuan tentang pupuk organik dari peserta yaitu tidak tahu sebanyak 4 peserta (9,5%), cukup tahu sebanyak 25 peserta (58%) dan sangat tahu sebanyak 14 peserta (32,5%). Sedangkan pemahaman tentang pemanfaatkan pupuk organic terhadap toga didapatkan hasil 29 peserta cukup paham (67,5%), 8 peserta sangat paham (18,5%) dan 5 peserta masih belum paham (12%). Permasalahan tanaman obat pada kelompok asuhan mandiri dapat diatasi dengan cara aplikasi pupuk organik berasal dari kotoran hewan domba yang telah diolah menjadi pupuk yang ramah lingkungan.
COUNSELING ON REPRODUCTIVE DISORDERS MANAGEMENT IN FEMALE BEEF CATTLE LIVESTOCK GROUPS IN DALEGAN VILLAGE, PANCENG DISTRICT, GRESIK REGENCY Suprayogi, Tri Wahyu; Susilowati, Suherni; Hermadi, Herry Agoes; Al Arif, M Anam; Hernawati, Tatik; Mulyati, Sri; Suwanti, Lucia Tri; Maslachah, Lilik; Kurnijasanti, Rochmah; Lestari, Tita Damayanti; Effendi, Mustofa Helmi; Hernanda, Ary Setya; Supriyadi; Saputra, Rakan Mahiid; Dewi, Aprilia Kurnia
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 9 No. 1 (2025): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v9i1.2025.026-038

Abstract

Management of reproductive disorders in female beef cattle (Bos taurus) is a vital step in enhancing livestock productivity and animal welfare. This educational activity was conducted in Dalegan Village, Panceng District, Gresik Regency, aiming to assist farmers in improving cattle reproductive performance through feed technology and supporting the development of modern farming practices. The methods applied included counseling, practical field demonstrations, and interactive discussions with participants, presented in a qualitative descriptive format. The results indicated an improvement in farmers' knowledge of the causes, clinical signs, and management steps for reproductive disorders such as anestrus, dystocia, and endometritis, as evaluated through pre-test and post-test assessments. Additionally, the activity provided guidance on the importance of feed management, cattle reproductive health, and modern farm management. In conclusion, this counseling successfully increased farmers' awareness of cattle reproductive health, which is expected to support the productivity of beef cattle farming in the region.
Co-Authors Adiana Mutamsari Witaningrum Agustina, Firanda Al Arif, M Anam Al arif, Mohammad Anam Al-Anshori, Akhmad Afifudin Andreas Berny Yulianto, Andreas Berny Arief Budiman Ayu, Zerlinda Dyah Boedi Setiawan Candika, Yossy Imam Chairul A. Nidom Chairul A. Nidom Chairul Anwar Chandra, Evania Haris Cholifah, Siti Chusnul Chusniati, Sri Dadik Rahardjo, Dadik David Mohamad Qadafi Dewi, Aprilia Kurnia Eko Agus Suyono Endang Suprihati Hani Plumeriastuti Hendarti, Gracia Angelina Hermin Ratnani Hernanda, Ary Setya Herry Agoes Hermadi Hidajati, Nove Hidanah, Sri Hidayatik, Nanik Hisyam, Mirza Atikah Madarina Indriyanti, Astrid Ismi, Alif Noviana Kadek Rachmawati Khoirunnisa, Itrah Koestanti S, Emy Kurnijasanti, Rahma Legowo, Djoko Loeki Enggar Fitri Lucia Tri Suwanti, Lucia Tri Luqman, Epy Muhammad Mahendra Pujiyanto Mirino, Mario Navyseal Mochamad Donny Koerniawan Mochamad Lazuardi Muhammad Hambal Munawer Pradana Mustofa Helmi Effendi Nunuk Dyah Retno Lastuti Nusdianto Triakoso Poedji Hastutiek Pratama, Abrian Panggalih Indra Pujiyanto, Mahendra Putri, Anandia Nafisah Raharjo, Hartanto Mulyo Rahmi Sugihartuti, Rahmi Ratna Damayanti Rochmah Kurnijasanti Rusyawardani, Aldis Ingrid Sabdoningrum, Emy Koestanti Saputra, Rakan Mahiid Saraswati, Afif Tasya Sarudji, Suryanie Soeharsono Soeharsono Sri Mulyati Suherni Susilowati Suhud, Mohamad Kharis Sukmanadi, Moh. Sukmanadi, Mohammad Sulaiman, Azhari Athaillah Supranianondo, Koesnoto Suprihati , Endang Supriyadi Suzanita Utama Tacharina, Martia Rani Tatik Hernawati Tintin Sukartini Sukartini, Tintin Sukartini Tita Damayanti Lestari Tri Wahyu Suprayogi Tyaningsih, Wiwiek Ulfah Juniarti Siregar Utomo, Gogik Satrio Margo Widiyatno, Thomas Valentinus Widjiati Widjiati, Widjiati Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Wiwik Misaco Yuniarti Yoes Prijatna Dachlan Yulianna Puspitasari Zhaza Afililla