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Cytokine profiles in dengue fever and dengue hemorrhagic fever: A study from Indonesia Masyeni, Sri; Wardhana, I Made W.; Nainu, Firzan
Narra J Vol. 4 No. 1 (2024): April 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i1.309

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Recent studies have demonstrated that cytokine dysregulation has a critical role in the pathogenesis of dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). The aim of this study was to investigate the association between tumor necrosis factor (TNF-α), interleukin 6 (IL-6), interleukin 10 (IL-10), and interleukin 17 (IL-17) with infection status, and severity of dengue. A prospective cross-sectional study was conducted at three hospitals in Gianyar regency and Denpasar municipality, Bali, Indonesia, from June to December 2022. Sixty-four dengue infected patients were involved. Patients’ serum was tested for dengue infection using NS1 antigen rapid test, dengue virus immunoglobulin M (IgM) and immunoglobulin G (IgG) test, and reverse transcription polymerase chain reaction (RT-PCR). Cytokine levels (TNF-α, IL-6, IL-10, and IL-17) were measured using enzyme-linked immunosorbent assay (ELISA). Infection status was determined by combining serological and RT-PCR results, categorizing patients into primary and secondary infections. The present study found that DF patients had lower TNF-α, IL-6, and IL-17 but higher IL-10 levels compared to DHF patients (p<0.001). Elevated TNF-α, IL-6, and IL-17 levels were higher in secondary infection, while IL-10 level was higher in primary infection (p<0.001). In conclusion, cytokines play a crucial role in the interplay between cytokine dysregulation and dengue infection dynamics.
Karakteristik Pasien Penderita Apendisitis Akut di RSUD Sanjwiani, Gianyar: Apendisitis akut MASYENI, SRI; SANJAYA LIE, MIRACLE NYOMAN; WIJAYA, INDRA
Hang Tuah Medical Journal Vol 22 No 1 (2024): Hang Tuah Medical Journal
Publisher : Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/htmj.v22i1.661

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Acute appendicitis is one of the most common emergency surgical cases of lower right abdominal pain, a small proportion of cases in emergency units in the world which are often experienced by children and adults. This study aims to identify the characteristics of acute appendicitis in patients at Sanjiwani Hospital, Gianyar. This research is a descriptive study with a retrospective cross-sectional approach method that uses secondary data in the form of medical records of acute appendicitis patients who underwent examinations at Sanjiwani Regional Hospital, Gianyar in the period 1 January 2020–31 December 2022. The results showed that the highest number of acute appendicitis sufferers were aged 10–19 years (34.3%), with the highest incidence in males (60.6%), and the most symptoms found, namely lower right abdominal pain (100%), with the most positive examination of the Mc Burney Sign (89.9%) and increased white blood cells with an average of 12.8 x 10^3/uL.
Karakteristik Pasien Dengan Penyakit Ginjal Kronis Stadium V Yang Menjalani Hemodialisis Reguler Di Rumah Sakit Tabanan MASYENI, SRI; MADE , SUHITA GIANI; PUTU, ARYA SURYANDITHA
Hang Tuah Medical Journal Vol 22 No 2 (2025): Hang Tuah Medical Journal
Publisher : Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/htmj.v22i2.810

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Abstract Chronic Kidney Disease (CKD) significantly impacts global health, contributing to both morbidity and mortality. This condition is characterized by severe kidney damage, which results in increased blood urea levels and can lead to serious complications. Reports indicate that the number of CKD cases is on the rise. This study is descriptive and employs a cross-sectional design, analyzing the medical records of CKD patients. The focus is on individuals who underwent examinations at Tabanan Hospital between January 1, 2019, and May 31, 2024. A total of 101 subjects were selected for the analysis. The results revealed that the majority of patients with stage V chronic kidney failure were over 60 years old (38.6%). The highest incidence of CKD was observed in men, accounting for 53.5% of the cases. Most patients had hypertension that was managed with medication (67.3%), and approximately 50.5% of the subjects experienced severe anemia
PEMBERDAYAAN KARYAWAN HOTEL FOURTEEN ROSES LEGIAN, DALAM PENCEGAHAN PENYAKIT INFEKSI SALURAN NAFAS BAWAH DALAM UPAYA PENCAPAIAN SUSTAINABLE DEVELOPMENT GOALS KETIGA: EMPOWERMENT OF FOURTEEN ROSES LEGIAN HOTEL EMPLOYEES, IN PREVENTION OF LOWER RESPIRATORY TRACT INFECTIONS IN AN EFFORT TO ACHIEVE THE THIRD SUSTAINABLE DEVELOPMENT GOALS Masyeni, Sri; Permana Lestari, Sagung Putri; Wedari, Niti
Jurnal Pengabdian Masyarakat Pesisir VOLUME 4 NOMOR 1
Publisher : Universitas Hang Tuah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/jpmp.v4i1.133

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The threat of various infectious diseases remains emerge in the world after COVID-19 pandemic. Public health response should be addressed to combating the infectious diseases threat. The community services are an important activity to achieve the third SDGs programme such as the prevention the lower tractus respiratory infection. This community partner was the Fourteen Roses Beach Hotel staff. The activities were training and counselling about the lower tractus respiratory infection. We report an increasing of the partners knowledge of prevention the lower tractus respiratory infection as of 70%. The current community service results will support the achievement of the third SDGs
Pengentasan Stunting di Lombok Barat Untuk mencapai Sustainable Development Goals Ketiga Masyeni, Sri; Yuniekawati, Yuyun; Lestarini, Ima Arum; Kadriyan, Hamsu; Sahadewa, Sukma; Rismawaty, Ika; Pinatih, Januartha Putra; Asparini, Ruby Riana
Jurnal Pengabdian Masyarakat Dharma Andalas Vol 4 No 1 (2025): Jurnal Pengabdian Masyarakat Dharma Andalas
Publisher : LPPM Universitas Dharma Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47233/jpmda.v3i2.1897

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Background: Stunting, a global issue, is being addressed by the World SDGs program, which has identified stunting alleviation as a key focus. Objectif: To enhance partner understanding of stunting, its treatment, and prevention. Methods: The community service method involves socialization through focus group discussions, counseling, and evaluation. Results: 100% of partners demonstrated an increase in their knowledge about stunting, with the average pretest score at 27 and the average posttest score at 83. Conclusion: Counseling, a practical and effective tool, significantly enhances knowledge about stunting.
NEUTROPHIL TO LYMPHOCYTE RATIO MEAN DIFFERENCE AMONG PEDIATRIC COVID-19 PATIENTS IN BALI: PRELIMINARY STUDY Maharani, Ni Luh Putu Silfia; Masyeni, Sri; Trisnia, Putu Ayunda; Paramasatiari, Anak Agung Ayu Lila
JURNAL WIDYA MEDIKA Vol. 11 No. 2 (2025): October
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v11i2.7703

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The Neutrophil-to-Lymphocyte Ratio (NLR) is a significant prognostic marker in COVID-19, reflecting the body's inflammatory immune response. This study aimed to analyze differences in mean NLR among paediatric COVID-19 patients in Bali, stratified by age and gender. A cross-sectional analytic study was conducted using data from 56 hospitalized paediatric patients at Sanjiwani Gianyar and Wangaya Hospitals. The sample comprised patients aged 0–18 years, distributed as follows: 0–5 years (41.1%), 5–10 years (32.1%), 10–15 years (21.4%), and 15–18 years (5.4%). The cohort included 31 males (55.4%) and 25 females (44.6%). Statistical analysis using the Kruskal-Wallis test revealed a significant difference in mean NLR across age groups (p = 0.037). Similarly, the Mann-Whitney test indicated a significant difference in mean NLR between genders (p = 0.004). These findings suggest that the inflammatory response to COVID-19 varies among paediatric patients by both age and gender. Consequently, clinicians should consider these demographic factors, as they may indicate a need for tailored therapeutic and monitoring strategies.
Karakteristik Berat Bayi Lahir Rendah di RSUD Tabanan I Komang Galih Vedanta Artana; Dewa Ayu Putri Sri Masyeni; Putu Arya Suryanditha
Reslaj: Religion Education Social Laa Roiba Journal Vol. 6 No. 7 (2024): RESLAJ: Religion Education Social Laa Roiba Journal 
Publisher : Intitut Agama Islam Nasional Laa Roiba Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47467/reslaj.v6i7.3734

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According to WHO, LBW is the condition of a baby with a birth weight of less than 2500 grams. LBW can occur due to maternal age, education, lack of nutritional intake, gap of pregnancy, parity, socioeconomic factors, fetal factors, and the placenta. This study aimed to determine the characteristics of LBW babies, maternal risk factors, and the average LBW in Tabanan Regional Hospital, Bali Province. A descriptive observational design with a cross-sectional approach on Tabanan Hospital medical record data 2020-2022 was used in this study. The sample was determined using consecutive sampling and 116 samples were acquired. The results showed that 87.9% of LBW babies were in the low category and 62.1% were male, most babies were born to mothers who were not at risk, 78.4% were from mothers aged 20-35 years, 65.5% were from mothers with multiparous parity, 78.4% were born at term pregnancy age, 77.6% were born with pregnancy gaps > 2 years, and 89.7% came from families without a history of LBW. Some babies were born to mothers with pregnancy complications, such as preeclampsia (15.5%), gestational hypertension (31.9%), anemia (28.4%), and premature rupture of membranes (24.1%). Some mothers had disease’s history. such as diabetes (9.5%), asthma (7.8%), hypertension (18.1%), HIV (2.6%), and syphilis (0.9%).
Pemberdayaan Masyarakat Sebagai Pembantu Jumantik di Desa Panjer Santosa, Agus; Widari, DAP Niti; Diari, Padma; Mahardika, Wayan Gede; Masyeni, Sri
Paradharma: Jurnal Aplikasi IPTEK Vol. 5 No. 2 (2021): Paradharma: Jurnal Aplikasi IPTEK
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Universitas Dhyana Pura – Bali

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (489.428 KB) | DOI: 10.36002/jpd.v5i2.1575

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ABSTRAKJumlah kasus infeksi demam berdarah dengue semakin meningkat dan memicu masalah kesehatan global. Pemerintah telah mencanangkan program satu Jumantik satu keluarga untuk menurunkan jumlah kasus penyakit demam berdarah dengue di Indonesia. Untuk membantu program pemerintah dalam menurunkan infeksi tersebut sangat diperlukan dukungan dan peran serta masyarakat. Pengabdian ini bertujuan untuk memberdayakan masyarakat sebagai pembantu Jumantik dalam identifikasi barang-barang yang memiliki potensi sebagai tempat sarang nyamuk pada saat musim penghujan. Mitra yaitu sekelompok ibu-ibu di Desa Panjer mempunyai masalah kurangnya pengetahuan mengenai infeksi demam berdarah beserta pencegahannya sehingga tidak mampu berperan sebagai pembantu Jumantik. Kegiatan pengabdian ini menawarkan solusi untuk memberikan pelatihan serta pendampingan untuk membentuk pembantu Jumantik dalam identifikasi barang bekas yang ada diseputar halaman rumah mitra yang memiliki potensi sebagai tempat tumbuhnay jentik nyamuk penyebar virus penyebab demam berdarah dengue. Kegiatan diawali dengan pret-est, pemaparan materi infeksi dengue serat pencegahannya lalu ditutup dengan evaluasi post-test untuk mengukur output kegiatan. Hasil kegiatan telah menunjukkan terjadi peningkatan pengetahuan mitra sebesar 90% dalam memahami penyakit serta pencegahan infeksi demam berdarah. Outcome kegiatan akan dievaluasi dari penurunan jumlah kasus infeksi demam berdarah di Desa Panjer.Kata kunci: pemberdayaan masyarakat, pembantu jumantik, infeksi dengueABSTRACTDengue virus infection poses a significant global health concern worldwide. A high number of dengue cases are reported annually in Indonesia and the government released a specific program to reduce the dengue cases. Empowering of the community to support the programme is absolutely needed. This programme aims to achieve the national programme in reducing the dengue cases particularly by helping the Jumantik. The main problems of the partner is the lack of dengue infection knowledge and the prevention methods. The program delivers training and mentoring to the partners about dengue infection prevention, and identifies the potential goods as the mosquito reservoir. The programme result is the improvement of the partner's knowledge, accounting as high as 90% improvement. The programme outcome will be evaluated by observing the reduction of the local dengue cases.Keywords: community empowering, jumantik, dengue
Faktor-Faktor yang Berpengaruh Terhadap Keparahan Infeksi Dengue pada Anak: English PUTU AYUNDA TRISNIA; SARASWATI LAKSMI DEWI; SRI MASYENI
Hang Tuah Medical Journal Vol 23 No 1 (2025): Hang Tuah Medical Journal
Publisher : Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/htmj.v23i1.844

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Dengue infection is one of the significant global health problems, especially in children. This paper aims to identify factors that affect the severity of dengue infection in the child population. The factors analysed included age, infection status, obesity, viral genome and Ns1 protein titers of dengue. Children who are younger and have an obese nutritional status tend to experience more severe symptoms. In addition, high NS1 titers, viral genomes, and secondary infections also contribute to a higher risk of infection. Detecting warning signs is important because it can be used as a predictor of more severe dengue infection.
Biofilm Formation in Staphylococcus aureus and Coagulase-Negative Staphylococcus Setiabudy, Marta; Masyeni, Dewa Ayu Putri Sri; Indraningrat, Anak Agung Gede; Suryawan, Kadek; Adhiputra, I Ketut Agus Indra; Rahman, Muhammad Amirul bin Abdul
Folia Medica Indonesiana Vol. 59, No. 3
Publisher : Folia Medica Indonesiana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Highlights: 1. The significance of Staphylococcus aureus and coagulase-negative Staphylococcus, which are more likely to infect immunocompromised patients, needed to be researched in greater depth. 2. Coagulase-negative Staphylococcus was found to form significantly more biofilm than Staphylococcus aureus. 3. Wound care and changing medical devices in immunocompromised patients on a regular basis may provide benefits to prevent biofilm formation by Staphylococcus spp. Abstract Staphylococcus spp. are typically commensal microorganisms that can exist in the human body without causing illness. However, these bacteria have virulence factors, e.g., biofilm formation, that are important to note. Because biofilms shield bacteria from opsonophagocytosis and antimicrobial agents, they can cause persistent or chronic infections. Once they form biofilms, both Staphylococcus aureus and coagulase-negative Staphylococcus (CoNS) can potentially cause incurable infections. This study aimed to compare biofilm formation in Staphylococcus aureus and coagulase-negative Staphylococcus as a guide for the prevention and management of infection, which will maintain and improve the good health of the general population. This was an analytic research with a cross-sectional design. The study began by collecting the samples, identifying the species, and testing the biofilm production with a microtiter plate, which was then analyzed with an enzyme-linked immunosorbent assay (ELISA). Data analysis was conducted using IBM SPSS Statistics for Windows, version 25.0 (IBM Corp., Armonk, N.Y., USA). Comparison tests were conducted using an independent t-test. A value of p<0.05 was used as the cut-off that indicated significance. The total samples were 36 clinical isolates, consisting of 18 Staphylococcus aureus and 18 coagulase-negative Staphylococcus. The specimens consisted of 20 blood samples (55.6%) and 7 wound swabs (19.4%). The biofilm test on the samples showed that 83.3% of the samples produced biofilms. The data revealed that the isolates formed biofilms, with 14 isolates (38.9%) in the strong category, 10 isolates (27.8%) in the moderate category, and each of 6 isolates (16.7%) in the weak and non-existent categories. Both Staphylococcus spp. appeared to have biofilm-forming activity, but coagulase-negative Staphylococcus appeared to be significantly more dominant (p=0.008). Strong biofilm was produced by 61.1% of coagulase-negative Staphylococcus isolates. In conclusion, coagulase-negative Staphylococcus formed a stronger biofilm than Staphylococcus aureus. Its presence as an infection-causing bacteria, particularly in immunocompromised patients, should not be underestimated.
Co-Authors A. Maheraditya A.A. Raka Budayasa AAG Indraningrat Adhiputra, I Ketut Agus Indra Agus Santosa Agus Santosa Agus Santosa Agus Santosa Ali A. Rabaan Anak Agung Gede Budhitresna, Anak Agung Gede Anak Agung Gede Indraningrat Ananda Maheraditya Andri Frediansyah Andri Frediansyah, Andri Arini, Ida A. Aryati , Aryati Aryati Aryati Aryati Aryati Aryati Aryati Atmaja, Kadek Surya Benediktus Yohan Benediktus Yohan Budhitresna, Anak AG. D. A. P. N. Widari D. G. A Budiyasa Danti, Ida Ayu Mahari Desak Putu Citra Udiyani Dewa Ayu Niti Widari Dewa Nyoman Sadguna Dewa Nyoman Sadguna Dewa Nyoman Sadguna Dewa P. Surawan DEWI, SARASWATI LAKSMI Diari, Padma Dinar, Gita Pritayanti Dinar, I Gusti Ayu Agung Gita Pritayanti Duwi Sumohadi ERLY SINTYA Erni J. Nelwan Erni J. Nelwan, Erni J. Erni Juwita Nelwan, Erni Juwita Fahriani, Marhami Firzan Nainu Firzan Nainu Francesco Rosiello Gondo Mastutik Gustu Rama Bhaskara Putra Hanifah Yusuf HANIFAH YUSUF Haypheng Te Helnida A. Maliga I Gede Pasek Mangku I Gusti Ngurah Adi Rajistha I Gusti Rai Tirta I Ketut Agus Indra Adhiputra I Komang Galih Vedanta Artana I Made Indra Wijaya I Made Sila Darmana I Made W. Wardhana Ida Bagus Semara Putra Ilmawan, Muhammad Ima Arum Lestarini Indraningrat, Anak Agung Gede Iqhrammullah, Muhammad Jonny K. Fajar Jonny K. Fajar Juwita, Dewa Ayu Putu Ratna K Irwan Setiawan Kadek Suryawan Kadriyan, Hamsu Kartika Dewi Kartika Dewi Keam, Synat Komang Aditya Yudistira Kuntaman Kuntaman Kuntaman Kuntaman Lestari, Putri P. Lestarini, Asri LUH VIDYA KENCANA S. ROBIN M Dharmesti Wijaya M. Indrawijaya MADE , SUHITA GIANI Made Dharmesti Wijaya Made Dharmesti Wijaya Maharani, Ni Luh Putu Silfia Mahardika, Wayan Gede Maliga, Helnida A. Margyaningsih, Nur Ita Marhami Fahriani Marta Setiabudy Md. Rabiul Islam Md. Rabiul Islam Miyazawa, Naori Muchlis AU Sofro Muchlis AU. Sofro Muhammad Amirul bin Abdul Rahman Muhammad Ilmawan Muhammad Iqhrammullah Ni Kadek Kumara Aida Ni Kadek Kumara Aida NI MADE DHANISWARA PUTRI WIRAWAN Ni Wayan Widhidewi Nindyaswari, Raka Dyah Nyoman Dian Permata Sari NYOMAN NGURAH PRIZKY ANGGRINA Ophinni, Youdiil P.A Suryanditha PA Suryandhita Paramasatiari, Anak Agung Ayu Lila Permana Lestari, Sagung Putri Permatananda, Pande Ayu Nayakasih Pinatih, Januartha Putra Prebawa, Arya Giri Purnomo, Windu Putri P. Lestari Putu Arya Suryanditha Putu Ayunda Trisnia Putu Indah Budi Apsari PUTU, ARYA SURYANDITHA R. Kartika R. Tedjo Sasmono R. Tedjo Sasmono Rabaan, Ali A. Rahman, Muhammad Amirul bin Abdul Ratna Kartika Retenra P. Suwari Rima Kusuma Ningrum Rismawaty, Ika Rosiello, Francesco Ruby Riana Asparini Salin Sirinam SANJAYA LIE, MIRACLE NYOMAN SARASWATI LAKSMI DEWI Sasmono, R. Tedjo Setiabudy, Marta Sirinam, Salin Sofro, Muchlis AU Sofro, Muchlis AU. SP Permana Lestari Sukma Sahadewa, Sukma Sumohadi, Duwi Surawan, Dewa P. Suryawan, Kadek Suwari, Retenra P. Synat Keam Te, Haypheng Trisnia, Putu Ayunda Usman Hadi Usman Hadi Vemi Hendrayani Wasudewa Wikananda Wedari, Niti Widari, DAP Niti Widhidewi, Ni W. Wijaya, Indra Wikananda, Wasudewa Windu Purnomo Wulandari, Christina Nyoman Yohan, Benediktus Youdiil Ophinni Yuniekawati, Yuyun