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EXPRESSION OF FOUR CYTOKINE/CHEMOKINE GENES IN PERIPHERAL BLOOD MONONUCLEAR CELLS INFECTED WITH DENGUE VIRUS Sri Masyeni, Dewa Ayu Putri; Hadi, Usman; Kuntaman, Kuntaman; Yohan, Benediktus; Margyaningsih, Nur Ita; Sasmono, R Tedjo
Indonesian Journal of Tropical and Infectious Disease Vol. 7 No. 4 (2019)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (193.265 KB) | DOI: 10.20473/ijtid.v7i4.7860

Abstract

Overproduction of numerous pro-inflammatory cytokines, during dengue virus (DENV) infection, has been related to plasma leakage in the vascular endothelium and studied elsewhere with conflicting results. The current study objective is to evaluate the expression of four cytokine/chemokine genes following DENV-2 infection within peripheral blood mononuclear cells (PBMC) isolated from a healthy donor. Venous blood was drawn,  and PBMCs were isolated using Ficoll density gradient centrifugation. Cells were maintained in culture medium and infected with Indonesian isolate of DENV-2. Cells were harvested and followed by total RNA extraction and reverse-transcription into cDNA using oligo d(T) primers and Reverse Transcriptase enzyme system. The SYBR Green-based quantitative qRT-PCR was used to calculate the relative expression of IL-6, IL-8, IP-10 and MIP-1β- encoding genes during infection time points, compared to uninfected cell controls. The observation of the cytokine was on the 6 and 18 hours post-infection. The different expression profiles of cytokines/chemokines were observed. The up-regulation of gene expression was observed for IL-8 and IP-10. In contrast, the down-regulatory of IL-6 and MIP-1β genes expression was documented during the infection period. The cytokine IL-8 and IP-10 are potent chemoattractants  in the recruitment  of neutrophil, basophil, and lymphocytes in response to an infection. The highlight of this study is on the up-regulation of IL-8 and IP-10 genes expression which may confirm the roles of these chemokines in the pathogenesis of dengue infection.
Tingkat Pemahaman dan Minat Wisatawan Domestik Terhadap Yoga Sebagai Aktivitas Pelayanan Pariwisata Wellness di Bali Gustu Rama Bhaskara Putra; Dewa Ayu Putri Sri Masyeni; I Made Indra Wijaya
Aesculapius Medical Journal Vol 4 No 2 (2024): June
Publisher : Fakultas Ilmu Kedokteran dan Ilmu Kesehatan, Universitas Warmadewa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22225/amj.4.2.2024.202 - 209

Abstract

[Level of Understanding and Interest of Domestic Tourists in Yoga as a Wellness Tourism Service Activity in Bali] Based on the Global Web Index report (2019), it is said that health tourism travel has experienced growth of 15.3% every year from 2015 to 2017, reaching 830 million trips annually. (8) Indonesia is in the 19th position out of 20 countries as a wellness tourism destination, according to the Global Wellness Tourism Economy 2020 report. (14) Currently, research related to wellness tourism has been intensively carried out, however research specializing in yoga has not been widely studied. This research aims to explore domestic tourists' understanding and interest in yoga as a wellness tourism service activity in Bali. This research is a descriptive study with a cross-sectional design. Implementation in December 2023 – March 2024 with a simple random sampling method. The data analysis used was univariate analysis. The number of samples in this study was 80 people. The research results showed that the characteristics of the respondents were dominated by 47 people (58.8%) who are female, 45 people aged 15 - 25 years (56.3%), 14 people from Jakarta (17.5%), and 37 students (46.3%). A total of 44 respondents really understood the general description of yoga (55.0%), and research respondents were dominated by respondents who often did yoga (more than once a week), as many as 29 people (36.3%). The level of understanding of yoga among research respondents was dominated by 51 respondents (63.8%) who understood yoga. The level of interest in yoga among research respondents was dominated by respondents who had a high level of interest in yoga, with 42 people (52.5%). This research is expected to provide information regarding domestic tourists' understanding and interest in yoga as a wellness tourism service activity in Bali.
PEMBERDAYAAN KARYAWAN HOTEL FOURTEEN ROSES LEGIAN, DALAM PENCEGAHAN PENYAKIT INFEKSI SALURAN NAFAS BAWAH DALAM UPAYA PENCAPAIAN SUSTAINABLE DEVELOPMENT GOALS KETIGA Masyeni, Sri; Permana Lestari, Sagung Putri; Wedari, Niti
Jurnal Pengabdian Masyarakat Pesisir VOLUME 4 NOMOR 1
Publisher : Universitas Hang Tuah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/jpmp.v4i1.133

Abstract

The threat of various infectious diseases remains emerge in the world after COVID-19 pandemic. Public health response should be addressed to combating the infectious diseases threat. The community services are an important activity to achieve the third SDGs programme such as the prevention the lower tractus respiratory infection. This community partner was the Fourteen Roses Beach Hotel staff. The activities were training and counselling about the lower tractus respiratory infection. We report an increasing of the partners knowledge of prevention the lower tractus respiratory infection as of 70%. The current community service results will support the achievement of the third SDGs
Pengentasan Stunting di Lombok Barat Untuk mencapai Sustainable Development Goals Ketiga Masyeni, Sri; Yuniekawati, Yuyun; Lestarini, Ima Arum; Kadriyan, Hamsu; Sahadewa, Sukma; Rismawaty, Ika; Pinatih, Januartha Putra; Asparini, Ruby Riana
Jurnal Pengabdian Masyarakat Dharma Andalas Vol 4 No 1 (2025): Jurnal Pengabdian Masyarakat Dharma Andalas
Publisher : LPPM Universitas Dharma Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47233/jpmda.v3i2.1897

Abstract

Background: Stunting, a global issue, is being addressed by the World SDGs program, which has identified stunting alleviation as a key focus. Objectif: To enhance partner understanding of stunting, its treatment, and prevention. Methods: The community service method involves socialization through focus group discussions, counseling, and evaluation. Results: 100% of partners demonstrated an increase in their knowledge about stunting, with the average pretest score at 27 and the average posttest score at 83. Conclusion: Counseling, a practical and effective tool, significantly enhances knowledge about stunting.
NEUTROPHIL TO LYMPHOCYTE RATIO MEAN DIFFERENCE AMONG PEDIATRIC COVID-19 PATIENTS IN BALI: PRELIMINARY STUDY Maharani, Ni Luh Putu Silfia; Masyeni, Sri; Trisnia, Putu Ayunda; Paramasatiari, Anak Agung Ayu Lila
JURNAL WIDYA MEDIKA Vol. 11 No. 2 (2025): October
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v11i2.7703

Abstract

The Neutrophil-to-Lymphocyte Ratio (NLR) is a significant prognostic marker in COVID-19, reflecting the body's inflammatory immune response. This study aimed to analyze differences in mean NLR among paediatric COVID-19 patients in Bali, stratified by age and gender. A cross-sectional analytic study was conducted using data from 56 hospitalized paediatric patients at Sanjiwani Gianyar and Wangaya Hospitals. The sample comprised patients aged 0–18 years, distributed as follows: 0–5 years (41.1%), 5–10 years (32.1%), 10–15 years (21.4%), and 15–18 years (5.4%). The cohort included 31 males (55.4%) and 25 females (44.6%). Statistical analysis using the Kruskal-Wallis test revealed a significant difference in mean NLR across age groups (p = 0.037). Similarly, the Mann-Whitney test indicated a significant difference in mean NLR between genders (p = 0.004). These findings suggest that the inflammatory response to COVID-19 varies among paediatric patients by both age and gender. Consequently, clinicians should consider these demographic factors, as they may indicate a need for tailored therapeutic and monitoring strategies.
Karakteristik Berat Bayi Lahir Rendah di RSUD Tabanan I Komang Galih Vedanta Artana; Dewa Ayu Putri Sri Masyeni; Putu Arya Suryanditha
Reslaj: Religion Education Social Laa Roiba Journal Vol. 6 No. 7 (2024): RESLAJ: Religion Education Social Laa Roiba Journal 
Publisher : Intitut Agama Islam Nasional Laa Roiba Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47467/reslaj.v6i7.3734

Abstract

According to WHO, LBW is the condition of a baby with a birth weight of less than 2500 grams. LBW can occur due to maternal age, education, lack of nutritional intake, gap of pregnancy, parity, socioeconomic factors, fetal factors, and the placenta. This study aimed to determine the characteristics of LBW babies, maternal risk factors, and the average LBW in Tabanan Regional Hospital, Bali Province. A descriptive observational design with a cross-sectional approach on Tabanan Hospital medical record data 2020-2022 was used in this study. The sample was determined using consecutive sampling and 116 samples were acquired. The results showed that 87.9% of LBW babies were in the low category and 62.1% were male, most babies were born to mothers who were not at risk, 78.4% were from mothers aged 20-35 years, 65.5% were from mothers with multiparous parity, 78.4% were born at term pregnancy age, 77.6% were born with pregnancy gaps > 2 years, and 89.7% came from families without a history of LBW. Some babies were born to mothers with pregnancy complications, such as preeclampsia (15.5%), gestational hypertension (31.9%), anemia (28.4%), and premature rupture of membranes (24.1%). Some mothers had disease’s history. such as diabetes (9.5%), asthma (7.8%), hypertension (18.1%), HIV (2.6%), and syphilis (0.9%).
Co-Authors A. Maheraditya A.A.G Indraningrat AAG Indraningrat Agus Santosa Agus Santosa Agus Santosa Ali A. Rabaan Anak Agung Gede Budhitresna, Anak Agung Gede Anak Agung Gede Indraningrat Ananda Maheraditya Andri Frediansyah Aryati Aryati Benediktus Yohan D A P Niti Widari D. A. P Niti Widari D. A. P. N. Widari D. G. A Budiyasa Dewa Nyoman Sadguna Dewa P. Surawan Duwi Sumohadi ERLY SINTYA Erni Juwita Nelwan, Erni Juwita Firzan Nainu Francesco Rosiello Gondo Mastutik Gustu Rama Bhaskara Putra HANIFAH YUSUF Haypheng Te Helnida A. Maliga I Ketut Agus Indra Adhiputra I Komang Galih Vedanta Artana I Made Indra Wijaya I Made Sila Darmana Ida Bagus Semara Putra IG Rai Tirta Ima Arum Lestarini Jonny K. Fajar K Irwan Setiawan Kadek Suryawan Kadriyan, Hamsu Kartika Dewi Kuntaman Kuntaman LUH VIDYA KENCANA S. ROBIN M. Indrawijaya Made Dharmesti Wijaya Made Dharmesti Wijaya Maharani, Ni Luh Putu Silfia Margyaningsih, Nur Ita Marhami Fahriani Marta Setiabudy Md. Rabiul Islam Muchlis AU Sofro Muhammad Amirul bin Abdul Rahman Muhammad Ilmawan Ni Kadek Kumara Aida NI MADE DHANISWARA PUTRI WIRAWAN Ni Wayan Widhidewi Nyoman Dian Permata Sari P.A Suryanditha PA Suryandhita Paramasatiari, Anak Agung Ayu Lila Permana Lestari, Sagung Putri Pinatih, Januartha Putra Putri P. Lestari Putu Arya Suryanditha Putu Indah Budi Apsari R. Kartika R. Tedjo Sasmono Ratna Kartika Retenra P. Suwari Rima Kusuma Ningrum Rismawaty, Ika Ruby Riana Asparini Salin Sirinam Sasmono, R. Tedjo SP Permana Lestari Sukma Sahadewa, Sukma Synat Keam Trisnia, Putu Ayunda Usman Hadi Vemi Hendrayani Wasudewa Wikananda Wedari, Niti Windu Purnomo Yohan, Benediktus Youdiil Ophinni Yuniekawati, Yuyun