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All Journal Buletin Udayana Mengabdi Indonesian Journal of Tropical and Infectious Disease WMJ (Warmadewa Medical Journal) KACANEGARA Jurnal Pengabdian pada Masyarakat WICAKSANA: Jurnal Lingkungan dan Pembangunan ISM (Intisari Sains Medis) : Jurnal Kedokteran Jurnal Kesehatan Indonesia Narra J Jurnal Abdimas Kartika Wijayakusuma Aesculapius Medical Journal Hang Tuah Medical Journal Jurnal Pengabdian Masyarakat Dharma Andalas Community Services Journal (CSJ) Paradharma: Jurnal Aplikasi IPTEK JPMP Surabaya Biomedical Journal RESLAJ: Religion Education Social Laa Roiba Journal Surabaya Biomedical Journal Jurnal Widya Medika Folia Medica Indonesiana
Sri Masyeni, Dewa Ayu Putri
Universitas Warmadewa
Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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Biofilm Formation in Staphylococcus aureus and Coagulase-Negative Staphylococcus Setiabudy, Marta; Masyeni, Dewa Ayu Putri Sri; Indraningrat, Anak Agung Gede; Suryawan, Kadek; Adhiputra, I Ketut Agus Indra; Rahman, Muhammad Amirul bin Abdul
Folia Medica Indonesiana Vol. 59, No. 3
Publisher : Folia Medica Indonesiana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Highlights: 1. The significance of Staphylococcus aureus and coagulase-negative Staphylococcus, which are more likely to infect immunocompromised patients, needed to be researched in greater depth. 2. Coagulase-negative Staphylococcus was found to form significantly more biofilm than Staphylococcus aureus. 3. Wound care and changing medical devices in immunocompromised patients on a regular basis may provide benefits to prevent biofilm formation by Staphylococcus spp. Abstract Staphylococcus spp. are typically commensal microorganisms that can exist in the human body without causing illness. However, these bacteria have virulence factors, e.g., biofilm formation, that are important to note. Because biofilms shield bacteria from opsonophagocytosis and antimicrobial agents, they can cause persistent or chronic infections. Once they form biofilms, both Staphylococcus aureus and coagulase-negative Staphylococcus (CoNS) can potentially cause incurable infections. This study aimed to compare biofilm formation in Staphylococcus aureus and coagulase-negative Staphylococcus as a guide for the prevention and management of infection, which will maintain and improve the good health of the general population. This was an analytic research with a cross-sectional design. The study began by collecting the samples, identifying the species, and testing the biofilm production with a microtiter plate, which was then analyzed with an enzyme-linked immunosorbent assay (ELISA). Data analysis was conducted using IBM SPSS Statistics for Windows, version 25.0 (IBM Corp., Armonk, N.Y., USA). Comparison tests were conducted using an independent t-test. A value of p<0.05 was used as the cut-off that indicated significance. The total samples were 36 clinical isolates, consisting of 18 Staphylococcus aureus and 18 coagulase-negative Staphylococcus. The specimens consisted of 20 blood samples (55.6%) and 7 wound swabs (19.4%). The biofilm test on the samples showed that 83.3% of the samples produced biofilms. The data revealed that the isolates formed biofilms, with 14 isolates (38.9%) in the strong category, 10 isolates (27.8%) in the moderate category, and each of 6 isolates (16.7%) in the weak and non-existent categories. Both Staphylococcus spp. appeared to have biofilm-forming activity, but coagulase-negative Staphylococcus appeared to be significantly more dominant (p=0.008). Strong biofilm was produced by 61.1% of coagulase-negative Staphylococcus isolates. In conclusion, coagulase-negative Staphylococcus formed a stronger biofilm than Staphylococcus aureus. Its presence as an infection-causing bacteria, particularly in immunocompromised patients, should not be underestimated.
Strategi Penurunan Angka Stunting Melalui Peningkatan Ketahanan Pangan di Dusun Pengalusan Desa Ban Karangasem Masyeni, Sri; Permatananda, Pande Ayu Nayakasih; Mangku, I Gede Pasek; Dinar, I Gusti Ayu Agung Gita Pritayanti; Rajistha, I Gusti Ngurah Adi; Dewi, Saraswati Laksmi; Miyazawa, Naori
Jurnal Abdimas Kartika Wijayakusuma Vol 6 No 4 (2025): Jurnal Abdimas Kartika Wijayakusuma
Publisher : LPPM Universitas Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/jakw.v6i4.723

Abstract

Stunting masih menjadi salah satu permasalahan kesehatan global yang perlu mendapat perhatian serius. Kondisi ini dapat bermula sejak masa kehamilan, berlanjut pada periode pertumbuhan anak, khususnya dalam 1000 hari pertama kehidupan. Penanggulangan stunting memerlukan pendekatan komprehensif yang menyasar berbagai faktor penyebabnya. Kegiatan pengabdian masyarakat ini bertujuan menekan prevalensi stunting di Desa Ban, Kecamatan Kubu, Kabupaten Karangasem, Bali. Metode yang diterapkan meliputi edukasi mengenai pencegahan dan penanganan stunting, praktik penyusunan menu bergizi seimbang melalui konsep “Isi Piringku”, serta penyaluran bantuan pangan berupa ayam buras, telur ayam, dan beras organik. Selain aspek kesehatan, kegiatan ini juga mencakup penyuluhan terkait perlindungan hukum bagi perempuan, pelatihan singkat Bahasa Inggris, serta pelatihan pembuatan pakan ternak ayam. Hasil kegiatan menunjukkan adanya peningkatan keterampilan ibu dalam menyiapkan makanan bergizi seimbang berbasis “Isi Piringku” sebesar 49,8%. Oleh karena itu, dukungan dan partisipasi masyarakat secara berkelanjutan sangat diperlukan untuk menurunkan angka kejadian stunting.
CHARACTERISTICS OF HYPERTENSION IN CHRONIC KIDNEY DISEASE PATIENTS UNDERGOING HEMODIALYSIS Danti, Ida Ayu Mahari; Masyeni, Sri; Dewi, Saraswati Laksmi; Lestarini, Asri
JURNAL WIDYA MEDIKA Vol. 12 No. 1 (2026): March
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v12i1.7871

Abstract

Hypertension in Chronic Kidney Disease (CKD) remains a global health issue, often presenting as resistant hypertension even with multiple antihypertensive medications. This study aims to describe the characteristics of hypertension in CKD patients undergoing hemodialysis at Sanjiwani Regional Hospital. A descriptive observational study with a cross-sectional design was conducted using secondary data from medical records of CKD patients with hypertension who underwent hemodialysis from January 2022 to December 2023, involving 100 patients. The study found that the highest proportion of hypertensive CKD patients were aged 46-65 years (60%), predominantly male (63%), with grade 1 hypertension (69%). The majority had CKD for ≥5 years (60%) and were taking ≤3 antihypertensive medications (72%). The commonly used antihypertensive drug class was calcium channel blockers (68%), with diabetes mellitus identified as the primary etiology (45%). Many CKD patients experience resistant hypertension, primarily affecting males aged 46-65 years.
CHARACTERISTICS OF PATIENTS WITH SNAKEBITES AT SANJIWANI HOSPITAL, BALI Nindyaswari, Raka Dyah; Masyeni, Sri; Dewi, Saraswati Laksmi; Juwita, Dewa Ayu Putu Ratna
JURNAL WIDYA MEDIKA Vol. 12 No. 1 (2026): March
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v12i1.8235

Abstract

Snakebites are a common occurrence in tropical regions, including Indonesia. This study aims to determine the demographic and clinical characteristics of patients bitten by snakes. It employs a descriptive, cross-sectional approach to analyze cases at Sanjiwani General Hospital. The sample includes all snakebite cases from January 2021 to December 2022, totaling 192 cases. The results indicate that the majority of snakebite incidents occurred in individuals aged 40-49 years (25.0%), predominantly among males (69.3%), and that most cases originated in Gianyar District (26.6%). The most frequently reported clinical symptom was pain (96.9%), with 91.1% of cases graded 2. Most patients did not experience complications (97.9%). Fang marks were the most common type of wound (96.9%), with the upper extremities being the most frequent bite site (51.6%). Laboratory examinations revealed that the average complete blood count was within the normal range, although prothrombin time (PT) and activated partial thromboplastin time (APTT) were prolonged. The treatments administered included antivenom (99.0%), Human Tetanus Immunoglobulin (94.3%), and antibiotics (99.5%). Notably, all patients in the study survived. The findings highlight the urgent need for targeted public health interventions to educate high-risk populations about snakebite prevention and the importance of seeking prompt medical treatment. Additionally, this study emphasizes the need for improved healthcare resources and training for medical personnel to manage snakebite cases effectively, thereby improving outcomes and reducing complications.
COMPARISON OF HBA1C LEVELS AMONG PATIENTS WITH TYPE 2 DIABETES MELLITUS WITH AND WITHOUT CHRONIC COMPLICATIONS AT BALI ROYAL GENERAL HOSPITAL Wulandari, Christina Nyoman; Atmaja, Kadek Surya; Masyeni, Sri Masyeni; Yudistira, Komang Aditya
JURNAL WIDYA MEDIKA Vol. 12 No. 2 (2026): July
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v12i2.8188

Abstract

Diabetes mellitus (DM) remains a major public health problem globally. Glycated hemoglobin (HbA1c) is widely used to assess long-term glycemic control in type 2 diabetes mellitus (T2DM), and persistent poor control may precipitate chronic microvascular and macrovascular complications. However, HbA1c profiles among hospitalized T2DM patients with established chronic complications in tertiary referral settings are still limited. This study compared HbA1c values between hospitalized T2DM patients with and without chronic complications at Bali Royal General Hospital. A cross-sectional analytic observational study was conducted using 130 inpatient T2DM medical records from 2022–2024 obtained through consecutive sampling. Descriptive variables included sex, age, antidiabetic regimen (oral vs injectable), and hypertension status. Chronic complications were categorized as macrovascular or microvascular based on clinical diagnoses documented in the medical records. Most patients were women (57.7%), with a mean age of 60.57 ± 10.41 years. Injectable therapy predominated (66.2%), and 58.5% had no documented hypertension. Patients with chronic complications had a higher median HbA1c than those without complications (8.60% [5.0–15.0] vs 7.50% [4.9–15.0]; p = 0.040). Overall, HbA1c levels were frequently above common treatment targets, indicating suboptimal long-term glycemic control in this inpatient cohort. Among hospitalized T2DM patients in a tertiary referral hospital, chronic complications were associated with significantly higher HbA1c levels, reflecting poorer long-term glycemic control. These findings highlight the need for sustained HbA1c optimization alongside comprehensive risk-factor management to mitigate long-term complication risk in referral settings.
The role of mediator suppressor of cytokine signaling (SOCS), toll-like receptor 3 (TLR-3) and nuclear factor kappa B (NFκB) on cytokine production during dengue virus infection Sri Masyeni; Kuntaman Kuntaman; Aryati Aryati; Muchlis AU. Sofro; Usman Hadi; Gondo Mastutik; Windu Purnomo; Agus Santosa; Muhammad Iqhrammullah; Benediktus Yohan; Erni J. Nelwan; R. Tedjo Sasmono
Narra J Vol. 3 No. 2 (2023): August 2023
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v3i2.167

Abstract

Inability to understand the pathogenesis of severe dengue, in particular the control mechanism of immune responses, has led to high mortality rate for patients with dengue shock syndrome (DSS). The aim of this study was to determine the control mechanism of cytokine production by mediator suppressor of cytokine signaling (SOCS), toll-like receptor 3 (TLR-3) and nuclear factor kappa B (NFκB) during DENV infection. Peripheral blood mononuclear blood cells (PBMC), isolated from healthy individuals, were infected with dengue virus (DENV)-2 strain SJN-006 Cosmopolitan genotype (isolated from Bali, Indonesia). The relative gene expression of SOCS-3, TLR-3, NFκB, and the cytokine genes (interleukin (IL)-6, IL-8, interferon inducible protein 10 (IP-10), and macrophage inflammatory protein-1 beta (MIP-1β)) were measured using qRT-PCR at 6, 12 and 24 hours post infection (hpi). Student t-test and Mann-Whitney test were used to compare the gene expressions while causal correlations were analyzed using regression test and path analyses. DENV-2 infection increased the gene expression of SOCS-3, TLR-3, and NFκB after 12 and 24 hpi. The expression of IL-6, IL-8, IP-10, and MIP-1β genes was increased and peaked at different times post-infection. NFκB and SOCS-3 genes likely have role in the upregulation of IL-8 and IL-6 gene expression, respectively. MIP-1β gene expression was significantly induced by both NFκB and SOCS-3. In conclusion, our study suggested that SOCS-3, TLR-3, and NFκB are important in regulating the production of IL-6, IL-8, IP-10, MIP-1β during early phase of DENV-2 infection. This enriches our understanding on pathogenesis pathway of DENV-associated cytokine storm.
Pemberdayaan masyarakat sebagai pendamping keluarga diabetes melitus untuk mencapai Sustainable Development Goals ketiga Masyeni, Sri; Dewi, Saraswati laksmi; Prebawa, Arya Giri; Dinar, Gita Pritayanti
KACANEGARA Jurnal Pengabdian pada Masyarakat Vol 9, No 2 (2026): Mei
Publisher : Institut Teknologi Dirgantara Adisutjipto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28989/kacanegara.v9i2.2880

Abstract

Dampak kesehatan global dari diabetes melitus telah diterima secara luas di berbagai tempat. Program pelayanan masyarakat yang menangani diabetes memainkan peran penting dalam meningkatkan kesehatan masyarakat dengan mempromosikan kesadaran, pencegahan, pengelolaan penyakit, dan pencegahan komplikasi. Tujuan dari pelayanan masyarakat ini adalah untuk meningkatkan pengetahuan para mitra dalam membimbing pasien. Metode program ini meliputi diskusi kelompok fokus untuk mengidentifikasi masalah mitra, penyuluhan pendidikan, permainan peran dengan tes pra dan pasca untuk mengevaluasi peningkatan, dan evaluasi program. Hasilnya menunjukkan peningkatan pengetahuan mitra tentang diabetes melitus sebesar 80%. Dengan melibatkan masyarakat dalam langkah-langkah kesehatan proaktif, program-program ini tidak hanya bertujuan untuk mengurangi prevalensi diabetes tetapi juga berupaya meningkatkan kualitas hidup bagi mereka yang terkena dampaknya, yang pada akhirnya berkontribusi pada komunitas yang lebih sehat dan pencapaian SDGs ke-3.
Co-Authors A. Maheraditya A.A. Raka Budayasa A.A.G Indraningrat AAG Indraningrat AAG Indraningrat Adhiputra, I Ketut Agus Indra Agus Santosa Agus Santosa Agus Santosa Agus Santosa Ali A. Rabaan Anak Agung Gede Budhitresna, Anak Agung Gede Anak Agung Gede Indraningrat Ananda Maheraditya Andri Frediansyah Andri Frediansyah, Andri Arini, Ida A. Aryati , Aryati Aryati Aryati Aryati Aryati Aryati Aryati Atmaja, Kadek Surya Benediktus Yohan Benediktus Yohan Budhitresna, Anak AG. D A P Niti Widari D. A. P Niti Widari D. A. P. N. Widari D. G. A Budiyasa Danti, Ida Ayu Mahari DAP Niti Widari DESAK PUTU CITRA UDIYANI Dewa Made Sadguna Dewa Nyoman Sadguna Dewa P. Surawan DEWI, SARASWATI LAKSMI Diari, Padma Dinar, Gita Pritayanti Dinar, I Gusti Ayu Agung Gita Pritayanti Duwi Sumohadi ERLY SINTYA Erni J. Nelwan Erni J. Nelwan, Erni J. Erni Juwita Nelwan, Erni Juwita Fahriani, Marhami Firzan Nainu Firzan Nainu Francesco Rosiello Gondo Mastutik Gustu Rama Bhaskara Putra Hanifah Yusuf HANIFAH YUSUF Haypheng Te Helnida A. Maliga I Gede Pasek Mangku I Gusti Ngurah Adi Rajistha I Ketut Agus Indra Adhiputra I Komang Galih Vedanta Artana I Made Indra Wijaya I Made Sila Darmana I Made W. Wardhana Ida Bagus Semara Putra IG Rai Tirta Ilmawan, Muhammad Ima Arum Lestarini Indraningrat, Anak Agung Gede Iqhrammullah, Muhammad Jonny K. Fajar Jonny K. Fajar Juwita, Dewa Ayu Putu Ratna K Irwan Setiawan Kadek Suryawan Kadriyan, Hamsu Kartika Dewi Kartika Dewi Keam, Synat Komang Aditya Yudistira Kuntaman Kuntaman Kuntaman Kuntaman Lestari, Putri P. Lestarini, Asri LUH VIDYA KENCANA S. ROBIN M Dharmesti Wijaya M. Indrawijaya MADE , SUHITA GIANI Made Dharmesti Wijaya Made Dharmesti Wijaya Maharani, Ni Luh Putu Silfia Mahardika, Wayan Gede Maheraditya, Ananda Maliga, Helnida A. Margyaningsih, Nur Ita Marhami Fahriani Marta Setiabudy Md. Rabiul Islam Md. Rabiul Islam Miyazawa, Naori Muchlis AU Sofro Muchlis AU. Sofro Muhammad Amirul bin Abdul Rahman Muhammad Ilmawan Muhammad Iqhrammullah Ni Kadek Kumara Aida NI MADE DHANISWARA PUTRI WIRAWAN Ni Wayan Widhidewi Nindyaswari, Raka Dyah Nyoman Dian Permata Sari NYOMAN NGURAH PRIZKY ANGGRINA Ophinni, Youdiil P.A Suryanditha PA Suryandhita Paramasatiari, Anak Agung Ayu Lila Permana Lestari, Sagung Putri Permana Lestari, SP Permatananda, Pande Ayu Nayakasih Pinatih, Januartha Putra Prebawa, Arya Giri Purnomo, Windu Putri P. Lestari Putu Arya Suryanditha Putu Ayunda Trisnia Putu Indah Budi Apsari PUTU, ARYA SURYANDITHA R. Kartika R. Tedjo Sasmono R. Tedjo Sasmono Rabaan, Ali A. Rahman, Muhammad Amirul bin Abdul Rai Tirta, IG Ratna Kartika RATNA KARTIKA Retenra P. Suwari Rima Kusuma Ningrum Rismawaty, Ika Rosiello, Francesco Ruby Riana Asparini Salin Sirinam SANJAYA LIE, MIRACLE NYOMAN SARASWATI LAKSMI DEWI Sasmono, R. Tedjo Setiabudy, Marta Sirinam, Salin Sofro, Muchlis AU Sofro, Muchlis AU. SP Permana Lestari Sukma Sahadewa, Sukma Sumohadi, Duwi Surawan, Dewa P. Suryawan, Kadek Suwari, Retenra P. Synat Keam Te, Haypheng Trisnia, Putu Ayunda Usman Hadi Usman Hadi Vemi Hendrayani Wasudewa Wikananda Wedari, Niti Widari, DAP Niti Widhidewi, Ni W. Wijaya, Indra Wikananda, Wasudewa Windu Purnomo Wulandari, Christina Nyoman Yohan, Benediktus Youdiil Ophinni Yuniekawati, Yuyun