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Em Sutrisna
Universitas Muhammadiyah Surakarta
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Journal : INDONESIAN JOURNAL OF PHARMACY

 1 
Antihyperurisemia activity of water extract of black seed (Coleus ambonicusLour) in balb-c mice and its standardization Andi Suhendi; Nurcahyanti .; Muhtadi .; EM Sutrisna
Indonesian Journal of Pharmacy Vol 22 No 2, 2011
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (221.619 KB) | DOI: 10.14499/indonesianjpharm0iss0pp77-84

Abstract

Effect  of  water  extract  of  black  seed  on  uric  acid  metabolism  in hyperuricemia mice models were studied. The influence of water extract of black seed  on  uric  acid  biosynthesis  was  xamined  using  mice  treated  by  250  mg/ body  weight  potassium  oxonate  intra  peritoneal.  The result  showed  that  water extract of black seed (200 mg/kg body weight, per oral) have a significant effect in reduction in plasma uric acid levels in comparison to negative control, but less active  than  positive  control  (allopurinol).  The  levels  of  uric  acid  in  mice  plasma for negative control, black seed extract and positive control were 3.100 ± 0.346 mg/dL,  0.200  ±  0.100  mg/dL  dan  1.200  ±  0.561  mg/dL,  respectively.  The standardization  performed  by  determined  specific  and  non  specific  parameters based  on  National  Bureau  of  Food  and  Drug,  Republic of  Indonesia.  Specific parameters  of  water  extract  of  black  seed  are  phenolic  content,  and  flavonoid content  are  0.664  ±  0.035  %  and  0.400  ±  0.018  %,  respectively.  Non  specific parameters of water extract of black seed are loss of weight, water content, ash content,  ash  insoluble  acid  content,  heavy  metals  contamination  (Pb  and  Cd), and  aflatoxin  residue  are  14.93-14.99%,  6.09-7.89%, 7.12-7.49%,  5.20-6.66%, 6.405 +0.211 ppm, 0.0096 +0, not detected, respectively.Key words: antihyperuricemia effect, water extract of black seed, standardization
Co-Authors . Muhtadi . MUHTADI Abdussalam, Arrizqi Hafidh Amalinda Ayu Faroska Andi Suhendi Andi Suhendi Andi, Andi Suhendi Andriani, Betty Anika Candrasari Annissa, Yanis Nur Arifah Sri Wahyuni Aris Fitriyani Ashshiddiiq, Zaid Ziyaadatulhuda Aulia, Qurrotu A’yuni Awal Prasetyo Ayustine Winarta, Selly Baiq Nova Fajriyatun Baiq Nova Fajriyatun, Baiq Nova Darnoto , Sri Dedi Hanwar DESY KURNIAWATI Desy Kurniawati Devi Usdiana Rosyidah Diana Risanti, Erika Dodik Nursanto Dona Dewi Nirlawati Dwiariawan Tauchid Rahman Dwiariawan Tauchid Rahman, Dwiariawan Tauchid Erna Kristin Esti Dwi Widayanti Faradisa, Nida Fardhiyanti, Fardhiyanti Faroska, Amalinda Ayu Fauziana , Eni Fauziana, Eni Flora Ramona Sigit Prakoeswa Frans Saputra Gunawan, Ryan Budi Hadi, Cahyono Happy Kurnia Permatasari Hasna Zahro Iftikhonsa Ihwan Susila Iin Novita Nurhidayati Mahmuda Ika Trisharyanti Dian Kusumowati Imronudin Imronudin, Imronudin Indra Nuroso Indwiani Astuti Iwan Dwiprahasto Iwan Dwiprahasto Kelik Wardiono Khaeditama Purnama Anwar Khusnul Khotimah Kurnia Retnowati Kurnia Retnowati, Kurnia Listiana Masyita Dewi Mada Sukma Dytho Maimun, Muhammad Halim Maisje Marlyn Kuhu Maryati Maryati Maryati Maryati Masyita Dewi, Listiana Maulindar, Joni Mida Pratiwi Muchlison Anis Mufidah, Fathin Muhammad Ibrahim Muhtadi Muhtadi Muhtadi MULYOTO PANGESTU Muzakar Isa N Nurhayani Noer Safita Noer Safita Nur Mahmudah Nurcahyanti . Nurcahyanti Wahyuningtyas Nurcahyanti Wahyuningtyas, Nurcahyanti Nurfauzia, Elita Nadia Nurhaliza, Alfida Aulia Rahma Firdauzy Nurhayani nurhayani Nurhayani Nurhayani Nurhayani, N Nurjanah, Adha Nuroso, Indra Peni Indrayudha Pranesti, Rahma Pratiwi, Betty Intan Purwani Rahmi , Anggraita Nur Rahmi, Anggraita Nur Ratih Pramuningtyas Ratnaningrum, Anna Retno Sintowati - Riandini Aisyah Rima Munawaroh Rini Kuswati Rochmadina Suci Bestari Ryan Budi Gunawan Safari Wahyu Jatmiko Safita, Noer Saputra, Frans Selly Ayustine Winarta Setyowatiningsih, Lilik Siti Zulaekah Sri Darnoto Studiviani Dwi Binuko, Raafika Sukma Dytho, Mada Suprapto . Suprapto . Surati Surati Tanti Azizah Sujono Usdiana Rosyidah, Devi Vianti, Melita Febry Wahyu Jatmiko, Safari Wardiono , Kelik Wasifah, Wasifah Werdany, Kusuma Estu Widananda , Clarisa Widananda, Clarisa Widiyanto, Santo Yoseph Didik Yanis Nur Annissa Yuli Kusumawati Yuni Prastyo Kurniati Yuniar, Siska Yusuf Alam Romadhon Zahro Iftikhonsa, Hasna Zaid Ziyaadatulhuda Ashshiddiiq