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All Journal Hemera Zoa Jurnal Ilmu Ternak Veteriner Partner Jurnal Pengabdian Masyarakat Peternakan ARSHI Veterinary Letters Veterinary Biomedical and Clinical Journal JURNAL KAJIAN VETERINER Jurnal Kedokteran Hewan Jurnal Lembaga Pengabdian Kepada Masyarakat Undana Jurnal Media Tropika: Jurnal Pengabdian Kepada Masyarakat Jurnal Veteriner Nusantara Jurnal Veteriner dan Biomedis Journal of Evidence - Based Nursing and Public Health Jurnal Info Kesehatan

Maxs Urias Ebenheizer Sanam

Universitas Nusa Cendana

Author-ID : 6312747

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary

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PERBANDINGAN NILAI TOTAL PLATE COUNT (TPC) DAN CEMARAN Salmonella sp. PADA IKAN TONGKOL (Eutynnus sp.) YANG DIJUAL DI TEMPAT PELELANGAN IKAN (TPI), PASAR TRADISIONAL DAN PEDAGANG IKAN ECERAN DI KOTA KUPANG Priska Clayu Apelabi; Diana A. Wuri; Maxs Urias E Sanam
JURNAL KAJIAN VETERINER Vol 3 No 2 (2015): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v3i2.1037

Tuna is one of the fishery products which has a high level of consumption in the city of Kupang but are easily damaged. One of the pathogenic bacteria in fish that cause food-borne disease is Salmonella sp. The contamination of Salmonella sp. is caused by inadequate sanitation, poor hygiene and contaminated water used by the merchant. This study aims to determine both the differences in the value of TPC and whether there is the contamination of Salmonella sp. or not. Moreover, this study was to examine quantification of contamination level and different levels of Salmonella sp contamination the difference level of Salmonella sp. contamination in tuna fishes which has been sold in the fish auction place (TPI), traditional markets and retail fish market in Kupang. 54 samples of Tuna were collected from three locations. This research was also supported by the water quality testing which consisted of 15 water samples. The result showed that there was a significant difference on the TPC values with an average level of contamination from the highest to the lowest, started from the fish retailers as many as 1,35 x 105 CFU/g, followed by the traditional markets as many as 5 x 104 CFU/g and TPI as many as 3,5 x 103 CFU/g. The differences of TPC values were due to the personal higyene and sanitation, locations, the equipments as well as the materials used in the post-arrest process, the distribution and the sales. The results from TPC analysis showed that 4 samples were contaminated by the Salmonella sp. The average level of Salmonella sp contamination was from the Tuna sold by the fish relailers as many as 1,6 x 102 followed by the traditional fish markets as many as 1 x 102 and in the TPI as many as 4x10. This study also showed that based on water quality test, positive results were found in 1 sample in TPI, 3 samples in the traditional markets and 4 samples in the fish retailers. In conclusion, the Tunas sold by the fish retailers have the highest TPC value and the highest level of Salmonella sp contamination compare to the Tunas sold in the traditional markets and TPI.

UJI SENSITIVITAS ANTIBIOTIKA TERHADAP Staphylococcus aureus YANG DIISOLASI DARI LUKA KULIT ANJING DI DESA MERBAUN, KECAMATAN AMARASI BARAT KABUPATEN KUPANG Eky Melyani Sanu; Maxs Urias E Sanam; Elisabet Tangkonda
JURNAL KAJIAN VETERINER Vol 3 No 2 (2015): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v3i2.1041

Staphylococcus aureus is one causes of wound infection in dogs, occured as secondary infection or trauma. One of the obstacles in the treatment of Staphycoccal infection is their resistance to antibiotics. This study aims to isolate and identify the Staphylococcus aureus in the cases of dog wound as well as testing the bacterial resistance to the antibiotics namely amoxicillin, tetracycline and ampicillin. There are total of 18 samples of dog wound taken by sterile swab and put in a closed test tube containing sterile physiological NaCl and stored in a ice jar. Swab is applied to the surface on Mannitol Salt Agar and observed morphology of the bacterial colonies that grow after incubated for 24 hours at 37 0C. Furthermore, the Gram staining, hemolysis test, biochemical tests including the catalase and coagulation were also tested. Suspect Staphylococcus aureus colonies were further tested for antibiotics sensitivity test using the Kirby Bauer’s method. Results of this experiment showed 8 isolates (44.44%) were positive to Staphylococcus aureus. The isolation of Staphylococcus aureus in the culture of Blood Agar showed that 8 isolates are Beta hemolytic strain. Antibiotics sensitivity test done in duplo revealed that there are 7 isolates of Staphylococcus aureus sensitive and 1 isolate resistant to ampicillin. The sensitivity test to the antibiotic tetracycline showed that 6 isolates sensitive and 2 isolates resistant. The sensitivity test to the antibiotic amoxicillin showed that 7 isolates sensitive and 1 isolates resistant. It can be suggested that in the treatment of wound infection in the dog's skin, antibiotic sensitivity test should be done, because some strains of Staphylococcus aureus has developed resistance to antibiotics.

PENGARUH PEMBERIAN DEKOK DAUN SIRSAK, KUNYIT PUTIH, DAN DAUN KERSEN SERTA KOMBINASINYA DALAM AIR MINUM TERHADAP PERFORMANS DAN KOLESTEROL DARAH AYAM PETELUR JANTAN YANG DIINFEKSI BAKTERI ESCHERICHIA COLI Erni Ferawati Kadja; J F BaleTherik; Maxs U. E. Sanam
JURNAL KAJIAN VETERINER Vol 6 No 1 (2018): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v6i1.2213

The purpose of this study was to determine the effect of soursop leaf (Annona muricata), saffron white (Curcuma mangga Val.) and cherry leaves (Muntingia calabura L.) and theirs combinations in drinking water on performances and blood cholesterol of laying roosters that infected by Escherichia coli. This study was designed using completely randomized design (CRD) with 11 treatments and 3 replications and each treatment using the 4 roosters. That obtained 33 experimental units. The treatments were the drinking water contain decoction of soursop leaves (DS), saffron white (KP) and cherry leaves (DK), and combinations were as follows: R0: without decoction of herbs (control), R1: 6% of DS decoction, R2: 6% of KP decoction, R3: 6 % of DK decoction, R4: 2% of DS decoction + 4% of KP decoction, R5: 2% of DS decoction + 4% of DK decoction, R6: 2% of KP decoction + 4% of DS decoction, R7: 2% of KP decoction + 4% of DK decoction, R8: 2% of DK decoction + 4% of DS decoction, R9: 2% of DK decoction + 4% of KP decoction, R10: 2% of DS decoction + 2% of KP decoction + 2% of DK decoction. Parameters of performance were: feed consumption, water intake, body weight gain, feed conversion. The result showed that the treatment had no effect on the performances (feed consumption, water consumption, body weight gain, feed conversion).

EVALUASI TITER ANTIBODI PASCA VAKSINASI Septicaemia epizootica PADA SAPI BALI DI KOTA KUPANG Mario H.Cantona; Maxs Urias Ebenheizer Sanam; Tri Utami; Tarsisius Considus Tophianong; Antin Y.N Widi
JURNAL KAJIAN VETERINER Vol 8 No 1 (2020): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v8i1.2292

Controlling Septicemia epizooticae (SE) through vaccination program has been undertaken in Kupang City. However, numbers of fatal cases are still being reported. The purpose of this study is to measure the antibody titer of Bali cattle after SE vaccination, and to determine the effect of age and sex on antibody titers. The 50 serum samples of SE vaccinated Bali cattle were taken from Alak Sub-district (26 samples) and Maulafa Sub-district (24 samples). The selection of sub-districts in Kupang City was taken in a simple random manner. Those serum samples were examined using the indirect enzyme-linked immunosorbent assay (ELISA) method. Antibody titers against SE is declared to be protective when the antibody titer is above 88 ELISA Unit (EU). Results indicated that average value of cattle antibody titer after the SE vaccination was able to trigger a protective antibody response (> 70 EU), meanwhile ONE WAY ANOVA analysis results showed that there is no significant effect (P> 0.05) of cattle age towards antibody titers. In the same way, the paired t test results did not indicate a difference in the value of antibody titers against the sex of the Bali cattle.

EVALUASI STATUS VIRULENSI ISOLAT Bacillus anthracis ASALNUSA TENGGARA DAN PAPUA MENGGUNAKAN METODE POLYMERASE CHAIN REACTION MULTIPLEX Maxs Urias Ebenhaizar Sanam; Widya Asmara; Agnesia Endang Tri Hastuti Wahyuni; Michael Haryadi Wibowo; Rahmat Setya Adji
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengevaluasi status virulensi 22 isolat Bacillus anthracis (B. anthracis) asal Nusa Tenggara dan Papua menggunakan metode polymerase chain reaction (PCR) multiplex dengan dua pasang primer nukleotida yang memiliki target amplifikasi gen spesifik pada kedua plasmid. Ektraksi DNA dilakukan dengan metode lisis panas. Pasangan primer PA5 dan PA8 digunakan untuk mengamplifikasi gen pagA pada pXO1, sedangkan pasangan primer 1234 F dan 1301 R mengamplifikasi gen capABC pada pXO2. Hasil reaksi PCR menghasilkan dua pita DNA berukuran sekitar 600 dan 800 bp pada 20 isolat. Namun, dua isolat lain, masing-masing hanya memiliki salah satu dari kedua ukuran pita DNA tersebut. Sebagian besar koleksi isolat asal Nusa Tenggara dan Papua (91%) masih memiliki kedua plasmid secara lengkap (pXO1+/2+) dan karena itu bersifat virulen, sedangkan dua isolat lain (9%) telah kehilangan salah satu plasmid virulennya sehingga bersifat avirulen. Disimpulkan bahwa PCR multiplex dengan dua pasang primer dengan target amplifikasi pada plasmid dapat digunakan untuk evaluasi status virulensi isolat B. anthraci.

BUDIDAYA IKAN AIR TAWAR DI LAHAN KERING DESA CAMPLONG II KECAMATAN FATULEU KABUPATEN KUPANG, NTT Cynthia Dewi Gaina; Filphin Adolfin Amalo; Maxs. U. E. Sanam; Frans Umbu Datta; Inggrid Trinidad Maha; Yohanes T. R. M. R. Simarmata; Tri Utami
Jurnal Media Tropika Vol 1 No 1 (2021): Jurnal Media Tropika
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/mediatropika.v1i1.3928

Increasing the economy of rural communities is a process of improving an economic effort to meet the needs of life through an empowerment system form that meets human needs by utilizing surrounding natural resources. Natural resources owned by rural communities are the potential to be developed to improve the economy of the village community. If freshwater aquaculture is well developed and managed properly, it will bring beneficial results in line with the increase in fulfillment of public’ requirements. The purpose of community service program activities in Camplong II Village, Fatuleu District, Kupang Regency was community empowerment through freshwater aquaculture using the pond method. The people of Camplong II village have limited access to obtaining fresh fish products as its location is around 45-50 km from Kupang city which is known for its fresh sea products. Thus, the activities implemented were training on the installation of a square pond, providing 500 fish seeds and fish feed for 2 periods, mentoring and monitoring the maintenance of this pond, and developing human resources. The results obtained: installation of several fish pond with a diameter of 3 meters and a height of 4.0 m, an increase in community knowledge and skills, an early fish harvest stocked from this period which can improve the welfare of the community in Camplong II Village, Kupang Regency. From the results obtained there is potential in the form of land, water sources, and facilities that can be empowered to increase fish water farming production.

THE COUNSELING AND TRAINING PROGRAM FOR TRADITIONAL CHICKEN FARMING OF KUB (KAMPUNG UNGGUL BALITBANGTAN) CHICKEN STRAIN AT TAFENA KUAN GROUP IN CAMPLONG II VILLAGE Tri Utami; Maxs Urias Ebenheizer Sanam; Inggrid Trinidad Maha; Dewi F. L. Djungu
Jurnal Media Tropika Vol 1 No 1 (2021): Jurnal Media Tropika
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/mediatropika.v1i1.4031

The counseling and training program for traditional chicken farming of KUB (Kampung Unggul Balitbangtan) chicken strain had been done at Tafena Kuan group in Camplong II Village. The purpose of implementing this program is community empowerment in developing local poultry farming businesses in order to overcome food insecurity, reduce dependency on imported poultry commodities, and build community independence in providing good quality protein sources of food. This program was carried out from April to December 2020 at Tafena Kuan group, located in Camplong II village, Kupang Regency of East Nusa Tenggara province. The Tafena Kuan group has 22 members of chicken farmers. The community service team provided assistance in the form of six KUB chickens, chicken feed, vaccines, vitamins, and worm medicine to each farmer. The counseling and training had been followed by all farmers. Farmers of the Tafena Kuan group built chicken cages independently using local materials and started running a KUB chicken breeding intensively.

ISOLASI, IDENTIFIKASI DAN UJI SENSITIVITAS ANTIBIOTIK TERHADAP PASTEURELLA MULTOCIDA ASAL SAPI YANG DIPOTONG DI RUMAH PEMOTONGAN HEWAN OEBA KUPANG Harrold Subu Taopan; Maxs U.E. Sanam; Elisabet Tangkonda
Jurnal Veteriner Nusantara Vol 1 No 1 (2016): JVN
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v1i1.925

Pasteurella multocida is a Gram-negative bacteria which are not able to grow on media MacConkey agar, cocobacill shaped, are facultative anaerobic and non-motile with the biochemical characteristics that have catalase and oxidase activity and ferment glucose but not ferment lactose. P. multocida is a normal flora in the respiratory tract of cattle. There are two serotypes of P. Multocida which cause disease in cattle that are serotype A causes Shippin fever and serotype B:2 causes septicaemia epizootica (SE). This study aimed to isolate and identify the P. multocida in cattle slaughte houses Oeba Kupang and to determine the level of sensitivity to multiple antibiotics. As much as 30 samples of tracheal swabs Bali cattle slaughtered in slaughter houses Oeba Kupang taken as samples. Samples are then isolated in media blood agar. Separate colonies were taken and stained with Gram stain and then cultured on MacConkey agar. Colonies tested with biochemical tests consisting of carbohydrate fermentation tests on triple sugar iron agar (TSIA), motility test on sulphite indole motility (SIM), catalase test on nutrient broth (NB) and the oxidase test on oxidase strip. The sensitivity of P. multocida were tested by inhibition zone to the antibiotic ampicillin, amoxicillin, sefoksitin, tetracycline and ciprofloxacin in the Muller Hinton Agar media (MHA). The results showed that P. multocida was isolated from one tracheal swab samples. This P. multocida isolates were sensitive to ciprofloxacin, while are resistant to ampicillin, amoxicillin, tetracycline and cefoxitin.

IDENTIFIKASI Salmonella Sp. DAN Escherichia coli PADA LALAT DI TEMPAT PENJUALAN DAGING PASAR NAIKOTEN KOTA KUPANG Theresia Setia Palus; Maxs U.E. Sanam; Annytha I.R. Detha
Jurnal Veteriner Nusantara Vol 1 No 1 (2016): JVN
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v1i1.927

The flesh is a source of protein from animal which have high nutrition value but are perishable. Salmonella sp. and Escherichia coli are pathogenic bacteria in flesh that can cause food-borne disease. The contamination of bacteria is cause by the lack of personal hygiene and sanitation so the flesh damaged easily by flies as the vector such as Musca domestica and Chrysomya megacephala. This study aims to determine the value of Total Plate Count (TPC) and whether the flies body surface are contaminated by Salmonella sp. and Escherichia coli. A total of 122 sample flies taken from butcheries. The result showed that all of samples were contaminated by the Salmonella sp. and Escherichia coli. The value of TPC showed that the average of level contamination from the highest to the lowest, started from 1,8 x 107 CFU/g, followed 7,5 x 106 CFU/g and 9,19 x 105CFU/g. In conclusion, flies take from meat sales have the high value of TPC and contaminated by Salmonella sp..and Escherichia coli.

ISOLASI, PREVALENSI DAN UJI SENSITIVITAS ANTIBIOTIK TERHADAP Escherichia coli SEROTIPE O157 PADA AYAM BURAS YANG DIPERDAGANGKAN DI PASAR TRADISIONAL DI KOTA KUPANG Nathasya Pelt; Maxs U.E. Sanam; Elisabet Tangkonda
Jurnal Veteriner Nusantara Vol 1 No 1 (2016): JVN
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v1i1.928

Escherichia coli is a Gram negative bacteria of cocobacill in ranging from 2,2 μm and 0,4-0,7μm and 0,8 μm diameter , occurring single or in pairs, flagella pertiricus motile, facultative anaerobic, and including to eneterobactericeae family. E. coli inhabits the lower ileum and large intestine of most vertebrates with colonization of the neonatal gastrointestinal tract occurring within hours of birth. E. coli O157:H7 is a zoonotic agent, can causes bloody diarrhea, hemolitycuremic syndrome (HUS) and trombotyc thrombocythopenic purpura (TTP) in human. Chicken was known as one important reservoir of E. coli O157:H7. The aims of this study were to isolate, prevalence and antibiotic sensitivity test of E. coli O157:H7 in local chickens are selling in the traditional market Kupang City. The bacteria was isolated by culturing the agent in Eosin Methylene Blue Agar (EMBA) medium, isolated from emba then used to Gram staining, Biochemical test, O157 Latex agglutination test and antibiotic sensitivity test. Results of study showed that 33 (41,25%) out of 80 chicken’s fecal samples were identified as E. coli O157. This E. coli O157 are sensitive to ciprofloxacin, ampicilin, amoxicillin, tetracycline and cefoxitin. ciprofloxacin in the Muller Hinton Agar media (MHA). The results showed that P. multocida was isolated from one tracheal swab samples. This P. multocida isolates were sensitive to ciprofloxacin, while are resistant to ampicillin, amoxicillin, tetracycline and cefoxitin.

Co-Authors Agnesia Endang Tri Hastuti Wahyuni Agus Saputra Agus Wijaya Aji Winarso Aji Winarso Aji Winarso Aji Winarso, Aji Aloysius Heryanto Wunda Alvin H Sunbanu Anderias Umbu Roga Andreas Umbu Jara Sipul Anjelina Lidia Bouk Annytha Ina Rohi Detha Antin Y.N Widi Bero, Catharina De Ricci Inye Bili, Feny A. L. Budhy Jasa Widyananta Cantona, Mario Herryaldo Dangga, Susana Dasor, Aurelia Yuliarty Carmila Debora Irene Weki Desmond Timothy Hurek Deta, Herlina Umbu Dewi F. L. Djungu Dewi F.L Djungu Dewi Fesbayanti Lestari Djungu Diana A. Wuri Diana A. Wuri Diana Agustiani Wuri Diana Miranti Rihi Dodi Darmakusuma Dondin Sajuthi Eky Melyani Sanu Elisabet Tangkonda Erni Ferawati Kadja Ewaldus Wera Filphin Adolfin Amalo Foeh, Nancy D. F. K. Frans Umbu Datta Fresensi Anggraini Date Meze Gaina, Cynthia Dewi Gomez, Marito Britos Gregorius Ronaldo Mau Kuru Harrold Subu Taopan Hilarius Langobelen Imanuel Benu J F BaleTherik Jayusman Arsiyanti Joesoef Jems Graham Bell Jois M. Jacob, Jois M. Julianty Almet Juni Claudia Dami Kallau, Novalino Harold Geoffrey Karolina Trifonia Diaz Larry Richard Wellem Toha Loe, Fhady R. Loe, Fhady Risckhy Lucyan Maria Azi Owa Milo Maha, Inggrid Trinidad Malelak, Marlin Cindy Claudya Mandala, Graziela Angelicha Manurung, Imelda F.E Manuwolu, Lucy Filipini Maria Aega Gelolodo Maria Geong Maria Kikementina Junersi Marianus Triyanto Sado Mario H.Cantona Mbula, Viktoriano Kingking Dalman Meity Marviana Laut Meity Merviana Laut Michael Haryadi Wibowo Murni, Theresia F. I. M. D. Nadya Daramuli Kale Nahak, Handrianus K. Nalley, Wilmientje Marlene-Mesang Nathasya Pelt Ndaong, Nemay A. Nina Inocensia Welndy Nobrihas, Risty Suryani Nubatonis, Marthen L.O. Olivia Maria Ujan Orolaleng, Katarina Keleka Paulina Jolanda Naif Priska Clayu Apelabi Putra Nugroho, Mega Perkasa Rahmat Setya Adji Rahmat Setya Adji Rizaldo Mariano Ludji Romula A Jemadi Salestin, Ledy Chehfzy Sanam, Viona Mariana Dewi Sera Marbella Christin Langgar Simarmata, Yohanes T. R. M. R. Sitompul, Yeremia Y. Sitompul, Yeremia Yobelanno Soge, Bergitha Tarsisius Considus Tophianong Tarsisius Considus Tophianong Tarsisius Considus Tophianong Tarsisius Considus Tophianong, Tarsisius Considus Telupere, Franky M. S. Theresia Setia Palus Toha, Larry R. W. Toha, Larry Richard Wellem Tophianong, Tarsisius C Tri Utami Tri Utami Tri Utami Tri Utami Tri Utami Tri Utami Weraman, Pius Widi, Antin Y. N. Widi, Antin Y.N Widya Asmara Wilmientje Marlene Mesang Nalley Winda Atika Tosi Wuri, Diana Wuri, Diana A. Yeremia Y. Sitompul Yohanes Naileta Koli Yohanes Simarmata Yohanes T. R. M. R Simarmata Yohanes T. R. M. R. Simarmata Yohanes T.R.M.R Simarmata Yohanes TRMR Si Yohanes TRMR Simarmata Yohanes TRMR Simarmata

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