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Pengaruh Aplikasi Vaksin Dan Suplementasi Probiotik Terhadap Titer Antibodi Dan Pertumbuhan Ayam KUB Yang Dipelihara Di Lingkungan Lahan Kering Sanam, Viona Mariana Dewi; Tangkonda, Elisabet; Sanam, Maxs U.E
Jurnal Veteriner Nusantara Vol 7 No 1 (2024): April, 2024
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v7i1.11719

Abstract

The maintenance of chicken livestock is often constrained by several infectious diseases that cause chicken death, one of which is Newcastle Disease. Efficiency in maintaining chicken production can be achieved with probiotics. Probiotic supplementation can reduce the risk of disease and improve chicken health. This study aims to determine the difference in antibody titer of KUB chickens given intramuscular vaccine and drinking water and to know the difference in body weight gain of chickens that get probiotics and those that do not get probiotics. The parameters observed were antibody titer before and after vaccination and body weight gain. This study used the HA and HI test methods. Positive results in the HA test are evenly distributed crystals (diffusion), while negative results in the HA test are flow (tears drop), while the HI test results can be observed by calculating the blood flow that occurs in each well. The results showed that there were significant differences in antibody titers between intramuscular and oral vaccination methods. The provision of probiotics did not show significant differences (P>0.05) on body weight gain of KUB chickens both those who received probiotics and those who did not receive probiotics.
EVALUASI STATUS VIRULENSI ISOLAT Bacillus anthracis ASALNUSA TENGGARA DAN PAPUA MENGGUNAKAN METODE POLYMERASE CHAIN REACTION MULTIPLEX Ebenhaizar Sanam, Maxs Urias; Asmara, Widya; Tri Hastuti Wahyuni, Agnesia Endang; Wibowo, Michael Haryadi; Adji, Rahmat Setya
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i2.2802

Abstract

Penelitian ini bertujuan mengevaluasi status virulensi 22 isolat Bacillus anthracis (B. anthracis) asal Nusa Tenggara dan Papua menggunakan metode polymerase chain reaction (PCR) multiplex dengan dua pasang primer nukleotida yang memiliki target amplifikasi gen spesifik pada kedua plasmid. Ektraksi DNA dilakukan dengan metode lisis panas. Pasangan primer PA5 dan PA8 digunakan untuk mengamplifikasi gen pagA pada pXO1, sedangkan pasangan primer 1234 F dan 1301 R mengamplifikasi gen capABC pada pXO2. Hasil reaksi PCR menghasilkan dua pita DNA berukuran sekitar 600 dan 800 bp pada 20 isolat. Namun, dua isolat lain, masing-masing hanya memiliki salah satu dari kedua ukuran pita DNA tersebut. Sebagian besar koleksi isolat asal Nusa Tenggara dan Papua (91%) masih memiliki kedua plasmid secara lengkap (pXO1+/2+) dan karena itu bersifat virulen, sedangkan dua isolat lain (9%) telah kehilangan salah satu plasmid virulennya sehingga bersifat avirulen. Disimpulkan bahwa PCR multiplex dengan dua pasang primer dengan target amplifikasi pada plasmid dapat digunakan untuk evaluasi status virulensi isolat B. anthraci.
Spatial Analysis of the Occurrence of Multi-Drug Resistance (MDR) Escherichia coli in Pet Dogs in Kupang City Kallau, Novalino H.G.; Sanam, Maxs U.E.; Utami, Tri; Sitompul, Yeremia Y.
JURNAL INFO KESEHATAN Vol 21 No 2 (2023): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol21.Iss2.1225

Abstract

Antibiotic resistance is an important health problem and a threat to public health. Dogs as pets can be both spreaders and reservoirs of antibiotic-resistant bacteria. Based on spatial analysis, this study aims to determine the distribution pattern of multi-drug resistance (MDR) Escherichia coli found in pet dogs in Kupang City. The spatial analysis method is based on the convex hull technique, elementary analysis of disease, and the Nearest Neighbour Index (NNI). Information on antibiotic-resistant E. coli has been obtained from the laboratory analysis results, and secondary data was obtained for spatial analysis. The results of this study have shown that the distribution pattern of the incidence of MDR E. coli in the City of Kupang in 2020 is a clustered pattern with the Nearest Neighbor Index (NNI) value of 0.783,  which is higher than the NNI AMR E. coli in the City of Kupang of 0.763. The incidence of MDR E. coli that has occurred in domesticated dogs takes place with a prevalence rate of 35% in an area of 5079 Ha. These results have shown that the incidence of MDR E. coli has spread to several areas in Kupang City and is a threat to public health. It requires appropriate prevention and control measures by implementing good hygiene and sanitation in the relationship between humans and their pets.
Co-Authors Agnesia Endang Tri Hastuti Wahyuni Agus Saputra Agus Wijaya Aji Winarso Aji Winarso Aji Winarso Aji Winarso, Aji Aloysius Heryanto Wunda Alvin H Sunbanu Anderias Umbu Roga Andreas Umbu Jara Sipul Anjelina Lidia Bouk Annytha Ina Rohi Detha Antin Y.N Widi Bero, Catharina De Ricci Inye Bili, Feny A. L. Budhy Jasa Widyananta Cantona, Mario Herryaldo Dangga, Susana Dasor, Aurelia Yuliarty Carmila Debora Irene Weki Desmond Timothy Hurek Deta, Herlina Umbu Dewi F. L. Djungu Dewi F.L Djungu Dewi Fesbayanti Lestari Djungu Diana A. Wuri Diana A. Wuri Diana Agustiani Wuri Diana Miranti Rihi Dodi Darmakusuma Dondin Sajuthi Eky Melyani Sanu Elisabet Tangkonda Erni Ferawati Kadja Ewaldus Wera Filphin Adolfin Amalo Foeh, Nancy D. F. K. Frans Umbu Datta Fresensi Anggraini Date Meze Gaina, Cynthia Dewi Gomez, Marito Britos Gregorius Ronaldo Mau Kuru Harrold Subu Taopan Hilarius Langobelen Imanuel Benu J F BaleTherik Jayusman Arsiyanti Joesoef Jems Graham Bell Jois M. Jacob, Jois M. Julianty Almet Juni Claudia Dami Kallau, Novalino Harold Geoffrey Karolina Trifonia Diaz Larry Richard Wellem Toha Loe, Fhady R. Loe, Fhady Risckhy Lucyan Maria Azi Owa Milo Maha, Inggrid Trinidad Malelak, Marlin Cindy Claudya Mandala, Graziela Angelicha Manurung, Imelda F.E Manuwolu, Lucy Filipini Maria Aega Gelolodo Maria Geong Maria Kikementina Junersi Marianus Triyanto Sado Mario H.Cantona Mbula, Viktoriano Kingking Dalman Meity Marviana Laut Meity Merviana Laut Michael Haryadi Wibowo Murni, Theresia F. I. M. D. Nadya Daramuli Kale Nahak, Handrianus K. Nalley, Wilmientje Marlene-Mesang Nathasya Pelt Ndaong, Nemay A. Nina Inocensia Welndy Nobrihas, Risty Suryani Nubatonis, Marthen L.O. Olivia Maria Ujan Orolaleng, Katarina Keleka Paulina Jolanda Naif Priska Clayu Apelabi Putra Nugroho, Mega Perkasa Rahmat Setya Adji Rahmat Setya Adji Rizaldo Mariano Ludji Romula A Jemadi Salestin, Ledy Chehfzy Sanam, Viona Mariana Dewi Sera Marbella Christin Langgar Simarmata, Yohanes T. R. M. R. Sitompul, Yeremia Y. Sitompul, Yeremia Yobelanno Soge, Bergitha Tarsisius Considus Tophianong Tarsisius Considus Tophianong Tarsisius Considus Tophianong Tarsisius Considus Tophianong, Tarsisius Considus Telupere, Franky M. S. Theresia Setia Palus Toha, Larry R. W. Toha, Larry Richard Wellem Tophianong, Tarsisius C Tri Utami Tri Utami Tri Utami Tri Utami Tri Utami Tri Utami Weraman, Pius Widi, Antin Y. N. Widi, Antin Y.N Widya Asmara Wilmientje Marlene Mesang Nalley Winda Atika Tosi Wuri, Diana Wuri, Diana A. Yeremia Y. Sitompul Yohanes Naileta Koli Yohanes Simarmata Yohanes T. R. M. R Simarmata Yohanes T. R. M. R. Simarmata Yohanes T.R.M.R Simarmata Yohanes TRMR Si Yohanes TRMR Simarmata Yohanes TRMR Simarmata