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ISOLASI, IDENTIFIKASI DAN UJI SENSITIVITAS ANTIBIOTIK TERHADAP PASTEURELLA MULTOCIDA ASAL SAPI YANG DIPOTONG DI RUMAH PEMOTONGAN HEWAN OEBA KUPANG Harrold Subu Taopan; Maxs U.E. Sanam; Elisabet Tangkonda
Jurnal Veteriner Nusantara Vol 1 No 1 (2016): JVN
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v1i1.925

Abstract

Pasteurella multocida is a Gram-negative bacteria which are not able to grow on media MacConkey agar, cocobacill shaped, are facultative anaerobic and non-motile with the biochemical characteristics that have catalase and oxidase activity and ferment glucose but not ferment lactose. P. multocida is a normal flora in the respiratory tract of cattle. There are two serotypes of P. Multocida which cause disease in cattle that are serotype A causes Shippin fever and serotype B:2 causes septicaemia epizootica (SE). This study aimed to isolate and identify the P. multocida in cattle slaughte houses Oeba Kupang and to determine the level of sensitivity to multiple antibiotics. As much as 30 samples of tracheal swabs Bali cattle slaughtered in slaughter houses Oeba Kupang taken as samples. Samples are then isolated in media blood agar. Separate colonies were taken and stained with Gram stain and then cultured on MacConkey agar. Colonies tested with biochemical tests consisting of carbohydrate fermentation tests on triple sugar iron agar (TSIA), motility test on sulphite indole motility (SIM), catalase test on nutrient broth (NB) and the oxidase test on oxidase strip. The sensitivity of P. multocida were tested by inhibition zone to the antibiotic ampicillin, amoxicillin, sefoksitin, tetracycline and ciprofloxacin in the Muller Hinton Agar media (MHA). The results showed that P. multocida was isolated from one tracheal swab samples. This P. multocida isolates were sensitive to ciprofloxacin, while are resistant to ampicillin, amoxicillin, tetracycline and cefoxitin.
IDENTIFIKASI Salmonella Sp. DAN Escherichia coli PADA LALAT DI TEMPAT PENJUALAN DAGING PASAR NAIKOTEN KOTA KUPANG Theresia Setia Palus; Maxs U.E. Sanam; Annytha I.R. Detha
Jurnal Veteriner Nusantara Vol 1 No 1 (2016): JVN
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v1i1.927

Abstract

The flesh is a source of protein from animal which have high nutrition value but are perishable. Salmonella sp. and Escherichia coli are pathogenic bacteria in flesh that can cause food-borne disease. The contamination of bacteria is cause by the lack of personal hygiene and sanitation so the flesh damaged easily by flies as the vector such as Musca domestica and Chrysomya megacephala. This study aims to determine the value of Total Plate Count (TPC) and whether the flies body surface are contaminated by Salmonella sp. and Escherichia coli. A total of 122 sample flies taken from butcheries. The result showed that all of samples were contaminated by the Salmonella sp. and Escherichia coli. The value of TPC showed that the average of level contamination from the highest to the lowest, started from 1,8 x 107 CFU/g, followed 7,5 x 106 CFU/g and 9,19 x 105CFU/g. In conclusion, flies take from meat sales have the high value of TPC and contaminated by Salmonella sp..and Escherichia coli.
ISOLASI, PREVALENSI DAN UJI SENSITIVITAS ANTIBIOTIK TERHADAP Escherichia coli SEROTIPE O157 PADA AYAM BURAS YANG DIPERDAGANGKAN DI PASAR TRADISIONAL DI KOTA KUPANG Nathasya Pelt; Maxs U.E. Sanam; Elisabet Tangkonda
Jurnal Veteriner Nusantara Vol 1 No 1 (2016): JVN
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v1i1.928

Abstract

Escherichia coli is a Gram negative bacteria of cocobacill in ranging from 2,2 μm and 0,4-0,7μm and 0,8 μm diameter , occurring single or in pairs, flagella pertiricus motile, facultative anaerobic, and including to eneterobactericeae family. E. coli inhabits the lower ileum and large intestine of most vertebrates with colonization of the neonatal gastrointestinal tract occurring within hours of birth. E. coli O157:H7 is a zoonotic agent, can causes bloody diarrhea, hemolitycuremic syndrome (HUS) and trombotyc thrombocythopenic purpura (TTP) in human. Chicken was known as one important reservoir of E. coli O157:H7. The aims of this study were to isolate, prevalence and antibiotic sensitivity test of E. coli O157:H7 in local chickens are selling in the traditional market Kupang City. The bacteria was isolated by culturing the agent in Eosin Methylene Blue Agar (EMBA) medium, isolated from emba then used to Gram staining, Biochemical test, O157 Latex agglutination test and antibiotic sensitivity test. Results of study showed that 33 (41,25%) out of 80 chicken’s fecal samples were identified as E. coli O157. This E. coli O157 are sensitive to ciprofloxacin, ampicilin, amoxicillin, tetracycline and cefoxitin. ciprofloxacin in the Muller Hinton Agar media (MHA). The results showed that P. multocida was isolated from one tracheal swab samples. This P. multocida isolates were sensitive to ciprofloxacin, while are resistant to ampicillin, amoxicillin, tetracycline and cefoxitin.
Kajian Penggunaan Antibiotik Pada Peternakan Babi Di Kecamatan Kota Raja Kota Kupang Debora Irene Weki; Maxs U.E Sanam; Tri Utami
Jurnal Veteriner Nusantara Vol 5 No 1 (2022): Februari 2022
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v5i1.3090

Abstract

The main cause of antibiotic resistance is the use of antibiotics that are misuse and overuse. Another factor is the misunderstanding of the use of antibiotics, the incorrect dosage of antibiotics, the many uses of broad-spectrum antibiotics and antibiotics that are sold freely without a doctor's prescription. This study aims to determine the percentage of antibiotic use among pig farms and the types of antibiotics used by pig breeders in the treatment of pigs in District of Kota Raja, Kupang City. A total of 80 respondents spread across eight sub-districts in District of Kota Raja, Kupang City. Information regarding the use of antibiotics was obtained through observation and structured interviews in the form of questionnaires distributed to pig farmers. Data were analyzed descriptively and then displayed in tables and figures. The percentage of pig farmers who use antibiotics in Kota Raja Subdistrict, Kupang City reaches 81.03% (47 farmers). The types of antibiotics used by pig breeders in District of Kota Raja, Kupang City are Oxytetracycline (57.45%); Sulfadimidine and Amoxicillin (6.38%) ; Procain Penicillin G and Dyhidrostreptomycine sulpphate (2.13%); Sulfadiazin sodium, Sulfadimine sodium and Sulfamerazinesodium (4.26%); and there was use of other types of antibiotics (23.4%) that were not identified by the farmer.
PENGARUH VARIASI DOSIS VAKSINASI ANTRAKS TERHADAP TITER ANTIBODI DAN TOTAL PROTEIN PLASMA PADA TERNAK DOMBA LOKAL Paulina Jolanda Naif; Maxs U. E. Sanam; Elisabet Tangkonda
Jurnal Veteriner Nusantara Vol 3 No 1 (2020): Februari 2020
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v3i1.3225

Abstract

Anthrax is an acute infectious disease caused by the bacterium Bacilus anthracis. This disease can attack domestic and wild animals, especially herbivorous animals, such as cattle, sheep, goats, sheep and can also attack humans. One of the preventive measures that can be taken is vaccination. In general, anthrax vaccination in livestock in Indonesia uses live spore vaccines, which contain B. anthracis 34F2 strain which is toxic, and is not encapsulated. Anthrax vaccination in goats and sheep can cause anaphylactic shock effects because it can still produce toxins. The incidence of anaphylactic shock in goats and sheep can be prevented by modifying the vaccination dose, namely the preinoculation dose. This study was conducted to determine whether there was a difference in the antibody titer value and the total plasma protein of sheep who received variations in the vaccination dose. There were 12 sheep that were divided into three groups, namely the control group, sheep that received the full dose of vaccination (0.5 cc) and sheep that received the preinoculation dose. Measurement of antibody titers was carried out using the indirect ELISA method and measurement of total plasma protein using a refractometer. The results of the analysis of variance (ANOVA) showed that there was no significant difference (P> 0.05) of the variation in the vaccination dose on antibody titer and total plasma protein. In general, the paired t test results did not show any difference in antibody titer values ​​at each sampling time. The antibody titer was negative until the 12th week after vaccination. The highest antibody titer value in the sheep group with the full dose was seen at week 12, while the highest titer in the sheep group with the preinoculated dose was seen at week 10
STUDI KERAGAMAN WARNA DAN MORFOMETRIK KUDA SANDELWOOD DI KABUPATEN SUMBA TENGAH Andreas Umbu Jara Sipul; Maxs U. E. Sanam; Budhy Jasa Widyananta
Jurnal Veteriner Nusantara Vol 3 No 2 (2020): Agustus 2020
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v3i2.3413

Abstract

The sandalwood horse is an important part of Sumba culture and tradition which has a high social and economic value. Sandalwood horses have their own characteristics, so they need to have their own standardization. The purpose of this study was to determine the dominance of sandalwood horse color and the distribution of facial marking, leg marking, body marking and morphometric images of sandalwood horses. Sampling using purposive sampling method with a sample size of 102 sandalwood horses. The results of this study indicate that the sandalwood horse has a color that is red or bay 22.56%, breath or chesnut 18.63%, black or black 14.71%, canusu or cremello 12.75%, dragem or brown 10.79%, rhyme or palomino 9.81%, monkey black or black. gray 4.91%, dawuk or gray 1.97%, albino; or 1.96% white, 0.98% white or skewbald stripes and 0.98% breath or roan stripes. facial marking dominated by star type 62.5% followed by star, strip, snip 12.5%, bald face 12.5% and snip 12.5%; leg marking was dominated by stockings 62.5% followed by half stocking 25% and pastern 12.5%; and body marking, there was a stamp on the cheek and shoulder location 49.1%, a stamp on the cheek and thigh location 29.41% and a single stamp on the horse's cheek location 21.56%. Morphometric data obtained from 63 Sandelwood horses, namely the average body weight of male horses 320.94 kg and female horses 286.76 kg. The average height of a mare is 124 cm and a male horse is 115 cm. The average body length of male horses is 107.26 cm and female horses are 116.82 cm. The average chest circumference of a male horse is 140.47 cm and that of a female horse is 159.21 cm.
PENGARUH PEMBERIAN Saccharomyces cerevisiae DAN Lactobacillus bulgaricus TERHADAP TITER ANTIBODI DAN PERTUMBUHAN PASCA VAKSINASI HOG CHOLERA PADA BABI Nina Inocensia Welndy; Maxs U. E. Sanam; Yohanes T. R. M. R Simarmata
Jurnal Veteriner Nusantara Vol 3 No 2 (2020): Agustus 2020
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v3i2.3423

Abstract

Classical Swine Fever (CSF) or hog cholera caused by classical swine fever virus (CSFV) is an important infectious disease in domestic pigs and wild pigs because this disease has a high mortality and morbidity rate in susceptible livestock. One effective way to prevent the spread of hog cholera is to vaccinate at the age of 30 days or 43 days, which is the weaning age of pigs. Weaning age is a critical time in a production system because of disturbances in nutrition, the immune system and the physiological system. A probiotic diet such as Saccharomyces cerevisiae and Lactobacillus bulgaricus was done to minimize losses. This study aims to determine the response of antibody titres to hog cholera vaccination and to determine the growth rate of body weight among pigs after giving S. cerevisiae and L. bulgaricus as additional feed. The research sample of 27 pigs aged one month were divided into three groups, namely group A without giving probiotics as control, group B for pigs with S. cerevisiae yeast flour (50mg / kgBB) and group C for pigs with L fermentation. . bulgaricus (3ml / kgBW). Serum samples for ELISA test were collected on day 0 and day 21 after vaccination. There was no significant difference (P> 0.05) in the antibody response before and after vaccination between S. cerevisiae and L. bulgaricus supplemental feeding due to high maternal antibodies. Body weight growth after giving S. cerevisiae yeast flour showed a significant difference (P<0.05) at day 0 to day 14 and day 14 to day 28 but did not show a significant difference (P > 0.05).
PENGARUH METODE APLIKASI VAKSINASI ANTRAKS TERHADAP SUHU TUBUH, FREKUENSI DENYUT JANTUNG DAN RESPIRASI DOMBA LOKAL Yohanes Naileta Koli; Maxs U. E. Sanam; Yohanes T. R. M. R Simarmata
Jurnal Veteriner Nusantara Vol 3 No 2 (2020): Agustus 2020
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v3i2.3424

Abstract

The anthrax disease is caused by Bacillus anthracis which is Gram- positive and rod-shaped. The disease attacks the herbivores and can also be transmitted in humans. Vaccination is one way that is used for the prevention of anthrax disease. The anthrax vaccinations still use live anthrax vaccine, containing Bacillus anthracis sprouts 34F2, toxigenic and not encapsulated and contain 10 million spores / ml in50% glycerine-NaCl physiologic solution and 0.5% saponin. The success of the anthrax vaccination program is strongly influenced bythe physiological status of the animal, the vaccine used and the givenmethod of administration and dosage. The World HealthOrganization recommends that the application of anthrax vaccine be done in two inoculation schedules of the first application of ¼ doses and then followed by full dose a month later. The side effects of vaccination reactions that lead to anaphylactic shock can be monitored and prevented by first measuring the physiological status of livestock in the form of temperature, pulsus and respiration in a certain period of time. The purpose of this study to determine whether there are differences in physiological parameters such as body temperature, heart frequency and respiration of sheep who received different doses of vaccination. 12 lambs were divided: first group of control, second group of full dose (0.5 cc) and third group of preinoculation dose (0.125 cc). Parameters measured in the morning and afternoon. Data analysis using variance Analysis. The results showed that there was no significant difference in physiological parameters of body temperature, heart frequency and local sheep respiration given different methods of vaccination..
Studi Literatur: Efektivitas Dan Keamanan Penerapan Desinfektan Secara Spraying Untuk Pencegahan Penularan Virus Corona (MERS, SARS, Dan SARS-COV-2) Fresensi Anggraini Date Meze; Maxs U.E Sanam; Tri Utami
Jurnal Veteriner Nusantara Vol 5 No 1 (2022): Februari 2022
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn/vol5iss1pp133-142

Abstract

Corona Virus is a zoonotic virus (transmitted form animal to human), which is cause disease from light to severe. There are two kind corona virus known in common that could be weight symptoms like Middle East Respiratory Syndrome (MERS-CoV) and Severe Acute Respiratory Syndrome (SARS-CoV). The purpose of compiling this literature study is to identify what kind of spraying disinfectant for prevent spreading, mechanism and safety, effectiveness/ impact spraying disinfectant about corona virus spreading. This literature study was obtained through searching and collecting from various reference sources using the Mendeley and Google Scholar applications. it was found that the type of spraying disinfectant that used to prevent coronavirus, include them were bleach solution (natrium hypochlorite), alcohol,povidone iodine, hydrogen peroxide. The mechanism of action of spraying disinfectants such as bleach solution (sodium hypochlorite) is to oxidize peptide bonds in cell membranes and denature proteins; alcohol disinfectants work by lysing the viral envelope; povidone iodine works by penetrating viral cell walls, oxidizing materials and dominating cells; hydrogen peroxide works by inhibiting the replication process, causing the death of the virus. Spraying disinfectants are quite effective in preventing transmission of the corona virus such as povidone iodine which is able to reduce virus titers and hydrogen peroxide which is able to inactivate the virus within 1 minute, but disinfectants sprayed in the environment or outdoors are not effective enough because they can be inactivated by dust and debris.
LAPORAN KASUS : LAMINITIS AKUT PADA BABI Alvin H Sunbanu; Yohanes Simarmata; Maxs U E Sanam
Jurnal Veteriner Nusantara Vol 4 No Supl. 1 (2021): Prosiding Seminar Himpro FKH Undana VI
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v4iSupl. 1.5988

Abstract

Seekor tenak babi Yorkshire berumur 7 bulan berjenis kelamin betina mengalami kepincangan pada salah satu kakinya. Berdasarkan hasil anamnesa dan pemeriksaan babi tersebut mengalami kepincangan dan terdapat lesi pada bagian kuku dari kaki serta terdapat kemerahan pada beberapa bagian tubuhnya. Selain itu, babi tidak makan dan hanya berbaring. Hasil pada awal pemeriksaan menunjukkan bahwa frekuensi napas : 28x/menit, frekuensi pulsus : 96x/menit dan suhu 40,5°C. Penanganan dilakukan dengan memberikan antibiotik Procaine Penicillin G (Penstep-400) dengan dosis sediaan 200 mg dan dosis anjuran 1 ml/kg BB dengan dosis pemberian 0,3 ml secara IM, d-panthonol (B-Sanplex) dengan dosis sediaan 10 mg dan dosis anjuran 1 ml/10 kg BB dengan dosis pemberian 6 ml secara IM dan Dexamethazone 0,75 mg secara peroral dengan dosis pemberian 4 tablet (2 tablet pagi dan 2 tablet sore) selama 3 hari. Monitoring pertama pada hari ke 3 suhu tubuh 40,8°C, frekuensi respirasi 32 x/menit, frekuensi pulsus 96x/menit dan tapi masih terlihat pincang serta masih terlihat adanya kebengkakan. Terapi antibiotik Procaine Penicillin G (Penstep-400) dan d-panthonol (B- Sanplex) dengan dosis yang sama dan tetap diberikan Dexamethasone. Pada monitoring kedua (hari ke-5) terlihat babi sudah berdiri secara normal dan tidak mengangkat kakinya namun, belum tidak terlalu aktif. Suhu tubuh 39,8°C, frekuensi respirasi 24x/menit, frekuensi pulsus 84 x/menit. Masih terlihat adanya pembengkakan pada kuku kaki dari babi tersebut. Monitoring ketiga pada hari ke-7 babi telah aktif bergerak dan nafsu makan meningkat, tidak terlihat adanya kebengkakan pada kakinya. Suhu 39,2°C, frekuensi respirasi 24x/menit, frekuensi pulsus 88x/menit.
Co-Authors Agnesia Endang Tri Hastuti Wahyuni Agus Saputra Agus Wijaya Aji Winarso Aji Winarso Aji Winarso Aji Winarso, Aji Aloysius Heryanto Wunda Alvin H Sunbanu Anderias Umbu Roga Andreas Umbu Jara Sipul Anjelina Lidia Bouk Annytha Ina Rohi Detha Antin Y.N Widi Bero, Catharina De Ricci Inye Bili, Feny A. L. Budhy Jasa Widyananta Cantona, Mario Herryaldo Dangga, Susana Dasor, Aurelia Yuliarty Carmila Debora Irene Weki Desmond Timothy Hurek Deta, Herlina Umbu Dewi F. L. Djungu Dewi F.L Djungu Dewi Fesbayanti Lestari Djungu Diana A. Wuri Diana A. Wuri Diana Agustiani Wuri Diana Miranti Rihi Dodi Darmakusuma Dondin Sajuthi Eky Melyani Sanu Elisabet Tangkonda Erni Ferawati Kadja Ewaldus Wera Filphin Adolfin Amalo Foeh, Nancy D. F. K. Frans Umbu Datta Fresensi Anggraini Date Meze Gaina, Cynthia Dewi Gomez, Marito Britos Gregorius Ronaldo Mau Kuru Harrold Subu Taopan Hilarius Langobelen Imanuel Benu J F BaleTherik Jayusman Arsiyanti Joesoef Jems Graham Bell Jois M. Jacob, Jois M. Julianty Almet Juni Claudia Dami Kallau, Novalino Harold Geoffrey Karolina Trifonia Diaz Larry Richard Wellem Toha Loe, Fhady R. Loe, Fhady Risckhy Lucyan Maria Azi Owa Milo Maha, Inggrid Trinidad Malelak, Marlin Cindy Claudya Mandala, Graziela Angelicha Manurung, Imelda F.E Manuwolu, Lucy Filipini Maria Aega Gelolodo Maria Geong Maria Kikementina Junersi Marianus Triyanto Sado Mario H.Cantona Mbula, Viktoriano Kingking Dalman Meity Marviana Laut Meity Merviana Laut Michael Haryadi Wibowo Murni, Theresia F. I. M. D. Nadya Daramuli Kale Nahak, Handrianus K. Nalley, Wilmientje Marlene-Mesang Nathasya Pelt Ndaong, Nemay A. Nina Inocensia Welndy Nobrihas, Risty Suryani Nubatonis, Marthen L.O. Olivia Maria Ujan Orolaleng, Katarina Keleka Paulina Jolanda Naif Priska Clayu Apelabi Putra Nugroho, Mega Perkasa Rahmat Setya Adji Rahmat Setya Adji Rizaldo Mariano Ludji Romula A Jemadi Salestin, Ledy Chehfzy Sanam, Viona Mariana Dewi Sera Marbella Christin Langgar Simarmata, Yohanes T. R. M. R. Sitompul, Yeremia Y. Sitompul, Yeremia Yobelanno Soge, Bergitha Tarsisius Considus Tophianong Tarsisius Considus Tophianong Tarsisius Considus Tophianong Tarsisius Considus Tophianong, Tarsisius Considus Telupere, Franky M. S. Theresia Setia Palus Toha, Larry R. W. Toha, Larry Richard Wellem Tophianong, Tarsisius C Tri Utami Tri Utami Tri Utami Tri Utami Tri Utami Tri Utami Weraman, Pius Widi, Antin Y. N. Widi, Antin Y.N Widya Asmara Wilmientje Marlene Mesang Nalley Winda Atika Tosi Wuri, Diana Wuri, Diana A. Yeremia Y. Sitompul Yohanes Naileta Koli Yohanes Simarmata Yohanes T. R. M. R Simarmata Yohanes T. R. M. R. Simarmata Yohanes T.R.M.R Simarmata Yohanes TRMR Si Yohanes TRMR Simarmata Yohanes TRMR Simarmata