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All Journal Hemera Zoa Jurnal Veteriner Jurnal Ilmu Ternak Veteriner Partner Jurnal Pengabdian Masyarakat Peternakan ARSHI Veterinary Letters Veterinary Biomedical and Clinical Journal JURNAL KAJIAN VETERINER Jurnal Kedokteran Hewan Jurnal Lembaga Pengabdian Kepada Masyarakat Undana Jurnal Media Tropika: Jurnal Pengabdian Kepada Masyarakat Jurnal Veteriner Nusantara Jurnal Veteriner dan Biomedis Journal of Evidence - Based Nursing and Public Health Jurnal Info Kesehatan
Maxs Urias Ebenheizer Sanam
Universitas Nusa Cendana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary

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STUDI KOMPARASI RESPON ANTIBODI PASCA VAKSINASI CLASSICAL SWINE FEVER PADA BABI LANDRACE DAN BABI LOKAL TIMOR DI KABUPATEN KUPANG, NUSA TENGGARA TIMUR Salestin, Ledy Chehfzy; Gelolodo, Maria Aega; Tangkonda, Elisabet; Sanam, Maxs U. E.; Bili, Feny A. L.
JURNAL KAJIAN VETERINER Vol 9 No 3 (2021): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v9i3.4956

Abstract

Classical Swine Fever (CSF) is an important infectious disease caused by the CSF virus, which belongs to the Pestivirus genus from the family Flaviviridae. This disease has a high fatality rate in swine. Stamping out the disease and vaccinations aimed at forming and strengthening antibodies against CSF are proven to be effective ways of disease control and prevention. Several factors, including age, immune status, health status, nutrition, and the environment, are believed to influence the success of the immunization program. Furthermore, it is assumed that different pig breeds may have impacts on vaccination success. This study aimed to compare antibody responses after CSF vaccination in two different pig breeds, Landrace and local Timor pigs. A total of 26 pigs (13 pigs for each group) was occupied in this study. All of the pigs used in this study had never been vaccinated against CSF. Antibody titers were quantified using an ELISA test, then the data were compared using an independent t-test to assess the difference of antibody responses between Landrace and local Timor pigs. Finally, the study found no significant difference in antibody response between Landrace and local Timor pigs, with a significant value of 0.07 (P > 0.05). Therefore, it is feasible to conclude that breed does not influence antibody response following CSF vaccination.
PRAKTIK MANAJEMEN PAKAN DAN RISIKONYA TERHADAP PENYEBARAN AFRICAN SWINE FEVER DI KOTA KUPANG Toha, Larry Richard Wellem; Sanam, Maxs U. E.; Gelolodo, Maria Aega
JURNAL KAJIAN VETERINER Vol 9 No 3 (2021): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v9i3.5477

Abstract

In early 2020 a high mortality of pigs in Malacca Regency, East Nusa Tenggara Province was confirmed as an ASF infection which then spread to all of Timor Island, Flores Island, and Sumba Island. One of the risk factors for the spread of ASF is swill feeding. The purpose of this study was to study the feed management practices of pig farmers during the ASF outbreak and to measure the relationship between feed management practices and the ASF outbreak in Kupang City. The study used a case-control study approach to study feed management practices for pig farmers during the outbreak and their relationship to ASF. A total of 69 farmers who had experienced the incidence of dead and sick pigs were taken as case samples and 21 farmers were randomly selected from around the area as controls. The data in this study were analyzed by descriptive statistics and bivariate analysis was used to measure the relationship between feed management practices and the incidence of ASF. The results of the bivariate analysis showed that swill feeding practice had a significant correlation with the incidence of ASF (P 0.038<0.05) with OR (2.94), while the factor of cooking or not cooking feed before being given to pigs was also significantly correlated with the incidence of ASF (P 0.003<0.05) with OR (0.1). In conclusion, based on epidemiological analysis, the factor of swill feeding practice that is not cooked is highly correlated with the incidence of ASF in Kupang City.
UJI POTENSI ANTIBAKTERI EKSTRAK ETANOL DAUN KAYU METANG (Lunasia amara blanco) TERHADAP Staphylococcus aureus Dasor, Aurelia Yuliarty Carmila; Sanam, Maxs U. E.; Ndaong, Nemay A.
JURNAL KAJIAN VETERINER Vol 9 No 3 (2021): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v9i3.5661

Abstract

Staphylococcus aureus is a normal flora in living things found on the skin and mucous membranes. Staphylococcus aureus can potentially cause infections of the skin and mammary glands. Natural ingredients that have the potential as antibacterial are Kayu Metang Leaves (Lunasia amara blanco) Manggarai people use the leaves to treat diabetes and wounds on the skin. The purpose of this study was to determine the secondary metabolites contained in Kayu Metang leaves and to determine the level of effective concentration as an antibacterial Staphylococcus aureus. This study used four replications and there were three variations of the ethanol extract concentration, namely 5%, 10%, and 20%. Data analysis in this study employed One-Way Anova followed by normality test and homogeneity test with 95% confidence level (a = 0.05). The results revealed that the inhibition zones resulting from concentrations of 5%, 10%, and 20% had different diameter values ​​and had the same average antibacterial strength, namely the strong category because the range of inhibition zones formed from 9.525 mm to 14.65 mm. The result of the one-way Anova test was a significance of p = 0.397. There was no significant difference in each concentration of metang wood leaf extract with a p value> 0.05. The use of metang wood extract had a smaller inhibitory power than the use of the antibiotic ciprofloxacin, but the use of metang wood extract has been shown to inhibit S. aureus bacteria. This study concluded that the leaves of metang wood (Lunasia amara blanco) have been shown to contain alkaloids, flavonoids, saponins, steroids and triterpenoids, and antibacterial compounds to the growth of Staphylococcus aureus.
Kuantifikasi Mikroorganisme dan Kelayakan Konsumsi Madu Lokal yang Diperjualbelikan di Kabupaten TTS (Timor Tengah Selatan) Bero, Catharina De Ricci Inye; Sanam, Maxs U. E.; Wuri, Diana A.
JURNAL KAJIAN VETERINER Vol 11 No 2 (2023): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v11i2.7557

Abstract

Honey is a product of animal origin with a low water composition but rich in sugar content (fructose and glucose) which is produced through the fermentation process in honey bee hives. This study aims to look at the quantification of microorganisms and the physicochemical quality of local honey which is traded in TTS (South Central Timor) Regency. The testing technique was carried out using the TPC test for total bacterial contamination and total mold and yeast contamination, testing the physical properties of honey using heating and organoleptic testing, and testing the chemical properties of honey which included testing water content, sugar content and pH. The test was carried out on 8 honey samples taken from 3 honey-producing villages in TTS Regency, namely Loli Village, Tobaki Village, and Nenas Village. The results of the total bacteria, mold and yeast test showed that the average bacterial contamination in honey was 7.24×105 and the average mold and yeast contamination was 30.31×105, where these results exceeded the contamination limit according to SNI No.7388:2009, namely the total contamination. bacteria <5×103 colonies/g and total mold and yeast contamination <1×101 colony/g. The physico-chemical properties test was carried out to find that the physical properties of honey were not in accordance with the SNI No.01-3545-2013 and SNI No.8664:2018.
POTENTIAL ANTIMICROBIAL IN WHEY AND LACTIC ACID BACTERIA IN SUMBA MARE'S MILK Sanam, Maxs U. E.
JURNAL KAJIAN VETERINER Vol 10 No 1 (2022): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v10i1.7868

Abstract

Sumba mare’s milk is rich in whey protein, fat, and lactic acid bacteria known to boost antimicrobial activities required in the prevention of diarrhea and inflammation. This study is, therefore, aimed at determining the antibacterial properties of whey and lactic acid bacteria as therapeutic compounds against pathogenic Staphylococcus aureus, SalmonellaTyphimurium, and Salmonella Enteritidis, respectively. The investigations were conducted at the Veterinary Public Health Laboratory of the Faculty of Veterinary Medicine, Nusa Cendana University. Whey antimicrobial test was performed using Salmonella Typhimurium ATCC® 19585 ™ and Staphylococcus aureus, while for lactic acid bacteria, SalmonellaEnteritidis ATCC® 13076 ™ obtained from the Veterinary Public Health Section, Faculty of Veterinary Medicine, Bogor Agricultural University, was applied. However, mare colostrum was acquired from East Sumba, East Indonesia. The results showed the average penicillin inhibitory zones of whey protein were estimated at 20 mm and 17 mm against Staphylococcus aureus. This also revealed the average diameter of the penicillin inhibitory zone and whey protein were equally evaluated at 2.1 mm, although the latter demonstrated sufficient antimicrobial activity against SalmonellaTyphimurium. Furthermore, the lactic acid bacteria inhibitory zone in filtrate and non-filtrates are strongly characterized using the agar method against Salmonella Enteritidis. In conclusion, whey protein and lactic acid bacteria from Sumba mare’s milk have significant antimicrobial potentials during the treatment of Salmonellosis and Staphylococcus aureusinfections.
GROSS PATHOLOGICAL FINDINGS OF AFRICAN SWINE FEVER SUSPECTS IN OEBELO, KUPANG REGENCY, 2021 Sanam, Maxs U. E.; Gelolodo, Maria Aega; Toha, Larry R. W.; Utami, Tri; Simarmata, Yohanes T. R. M. R.; Murni, Theresia F. I. M. D.
JURNAL KAJIAN VETERINER Vol 9 No 3 (2021): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v9i3.7869

Abstract

African swine fever (ASF) is a destructive re-emerging swine disease that has posed a serious economic threat to the global pig farming sector. In past years, ASF has rapidly spread over Europe, Asia, and Oceania, and begin to enter Indonesia in the middle of 2019. The clinical and pathological symptoms of ASF are influenced by the strain's virulence, the transmission pathway, and the pig's immunological and health status. ASF’s clinical manifestations are known to evolve, from after an invasion enters a new free region to after the disease has been established in the territory for a longer period. Identifying ASF clinical signs and pathological changes is crucial for a comprehensive and reliable early detection system. The objective of this research is to observe and identify gross pathology in ASF suspect pigs in order to obtain a better understanding of the cause of death. Two dead pigs from a farm in Oebelo village, Kupang regency, Indonesia with a recent history of massive deaths had been examined in this study. The post-mortem results showed that hemorrhagic splenomegaly and hemorrhagic lymphadenitis were the main lesions observed at the examinations. Furthermore, hemorrhages were also found in various internal organs such as the kidneys, liver, and heart. To determine the exact cause of the pigs' deaths, a molecular diagnostic test should be conducted.
INTRODUCTION STUDY OF MDR STAPHYLOCOCCUS AUREUS IN PIG SLAUGHTER HOUSE IN KUPANG CITY Sanam, Maxs U. E.; Kallau, Novalino H. G.; Sitompul, Yeremia Y.
JURNAL KAJIAN VETERINER Vol 10 No 1 (2022): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v10i1.7870

Abstract

Due to the unprudent use of antibiotics globally, it causes the incidence of antibiotic resistance with the emergence of bacteria that are resistant to antibiotics and has implications for the failure of the use of antibiotics in dealing with various cases of diseases caused by bacteria. The incidence of antibiotic resistance in the livestock sector occurs due to the use of antibiotics that are not based on recommendations and antibiotics are given in the long term. The presence of bacteria that carry resistance to one or several kinds of antibiotics in livestock is a threat to animal and human health. The city of Kupang as an area that has pig farms and a high pig population has a big challenge to the emergence of antibiotic resistance due to the use of antibiotics in maintaining livestock health. One of the bacteria that has a large number of distributions and is widely found in livestock and is an indicator of antibiotic resistance is Staphylococcus aureus (S. aureus). This research has used an observational research method based on data collection methods and laboratory examinations, which are adjusted to the objectives that have been set. The approach that has been used in this research is a Cross Sectional Study, because it wants to get an overview of S. aureus bacteria that are resistant to antibiotics. The sample that has been needed is pig feces obtained by field observations. The data analysis technique that has been used is a descriptive analysis model, to explain the presence of antibiotic-resistant S. aureus bacteria in pig farms. The results showed that this isolation and identification process resulted in 28 (51.85%) samples that were positive for S. aureus. The highest prevalence of resistance was found in S. aureus which was resistant to the antibiotic Colistin sulfate (82.1%). The number of antibiotics tested from S. aureus isolates showed various variations from 0 to 3 types of antibiotics that were resistant in 1 isolate. The most common resistance pattern shown in S. aureus isolates was the CS (Colistin sulfate) pattern as many as 19 of the 28 samples. The conclusion of this study encourages the need to increase supervision related to the use of antibiotics and increase public awareness of the use of antibiotics and prevention of the increasing incidence of antibiotic resistance.
Analisis Nukleotida dan Homologi Sekuens Fragmen Gen p72 (B646L) Virus African Swine Fever Virus (ASF) Asal Kota Kupang Sanam, Maxs U. E.; Gelolodo, Maria Aega; Toha, Larry R. W.
JURNAL KAJIAN VETERINER Vol 10 No 2 (2022): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v10i2.7875

Abstract

African Swine Fever (ASF) is an important infectious disease in pigs caused by African Swine Fever Virus (ASFV). Despite not being zoonotic, this disease has the potential to severely affect the socioeconomic conditions in the impacted regions. The majority of pig farmers in Indonesia, particularly those in Kupang City, that raise pigs in backyards or on a small scale, experience the impact of ASF's effects. Early in 2020, the ASF cases were confirmed in Timor Island, including the Kupang City area in Nusa Tenggara Timur (NTT) Province. The molecular information on ASFV in this area is still limited. In order to determine the homology and nucleotide analysis using BLAST NCBI, the ASFV p72 (B646L) gene fragment sequence from Kupang City was compared to ASFV p72 (B646L) gene segments from other parts of Indonesia and several other Asian countries. The results of nucleotide analysis and sequence homology of the original ASFV p72 (B646L) gene fragment from Kupang City showed a high level of homology to the ASFV p72 (B646L) gene fragment from West Java, North Sumatra, and several Asian countries. The findings from this study indicate that the source of ASF viral transmission across different regions may be comparable. Therefore, to prevent the dissemination of ASF, strict biosecurity measures must be implemented along with monitoring of animal and product transportation.
Respon Vaksinasi ND Ayam IPB D1 yang Dipelihara pada Lingkungan Lahan Kering Mandala, Graziela Angelicha; Tangkonda, Elisabet; Sanam, Maxs U. E.
JURNAL KAJIAN VETERINER Vol 11 No 2 (2023): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v11i2.8249

Abstract

Newcastle Disease (ND) is one of the diseases that causes large losses in the poultry farming industry. Vaccination can reduce symptoms and protect chickens from ND. High maintenance temperatures on dry land can interfere with immune cell production. IPB D1 chicken is a chicken resulting from a cross between local chickens and purebred chickens that is resistant to disease. The aim of the research was to determine the response of IPB D1 chicken vaccination against Newcastle Disease (ND) and compare the antibody titers of IPB D1 chickens in tropical regions that were vaccinated with ND via the drinking water and intramuscular routes. This research was carried out on 30 IPB D1 chickens divided into three groups, namely , group A vaccination via drinking water, group B vaccination via intramuscular and control group (C) with the aim of comparing the antibody titers of IPB D1 chickens vaccinated via drinking water and intramuscularly and to determine the response of vaccination of IPB D1 chickens against Newcastle Disease. Pre- and post-vaccination immune responses were tested using hemagglutination inhibition (HI). The results showed that the group of chickens vaccinated through drinking water had an average pre-vaccination antibody titer of 1.8 ± 0.91 log 2, at week 2 post-vaccination it was 3.3 ± 2.05 log 2, at week 4 post-vaccination it was 5 .3 ± 2.31 log 2 and at the 8th week post-vaccination 3.2 ± 1.22 log 2. The average antibody titer in the group of chickens vaccinated via intramuscular pre-vaccination was 1.6 ± 0.51 log 2, at week 2. -2 post-vaccination 5.4 ± 3.09 log 2, at the 4th week post-vaccination 4.89 ± 2.71 log 2 and at the 8th week post-vaccination 3.56 ± 2.01. It was concluded that ND vaccination via the drinking water and intramuscular route was able to increase antibodies to reach an average titer above 4 HI log 2 within a period of at least four weeks after administering the vaccine to IPB D1 chickens kept in a dry land environment. The antibody titer of IPB D1 chickens vaccinated intramuscularly increased faster than those vaccinated through drinking water.
IDENTIFIKASI MORFOLOGI LARVA Anisakis sp PADA Epinephelus sp. DAN Rastrelliger sp DI PERAIRAN NTT Sanam, Maxs U. E.; Detha, Annytha; Wuri, Diana; Dangga, Susana
JURNAL KAJIAN VETERINER Vol 9 No 2 (2021): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v9i2.8425

Abstract

The purpose of this research is to identify the level of intensity and degree of infection incidence of Anisakis sp in Rastrelliger sp and Epinephelus sp in eastern waters of Indonesia. A total of 190 fish specimens were randomized (random sampling) on ​​95 Rastrelliger sp and 95 Epinephelus sp from fish selling places in various areas of Kupang city. The larvae obtained were collected and fixed with 70% alcohol, and stained using Semichen acetic carmine solution. Morphological identification of Anisakis sp larvae through ventriculus, boring tooth, and mucron shapes on a stereo microscope. The parasites found were grouped into Anisakis sp type I and type II. Based on the results of the study, it was found that the presence of Anisakis sp. in Epinephelus sp that is 94 out of 95 fish. The results also confirmed that Anisakis sp infection implies a high risk of Epinephelus sp fish species so that further identification is needed at the molecular level.
Co-Authors Agnesia Endang Tri Hastuti Wahyuni Agus Saputra Agus Wijaya Aji Winarso Aji Winarso Aji Winarso Aji Winarso, Aji Aloysius Heryanto Wunda Alvin H Sunbanu Anderias Umbu Roga Andreas Umbu Jara Sipul Anjelina Lidia Bouk Annytha Ina Rohi Detha Antin Y.N Widi Bero, Catharina De Ricci Inye Bili, Feny A. L. Budhy Jasa Widyananta Cantona, Mario Herryaldo Dangga, Susana Dasor, Aurelia Yuliarty Carmila Debora Irene Weki Desmond Timothy Hurek Deta, Herlina Umbu Dewi F. L. Djungu Dewi F.L Djungu Dewi Fesbayanti Lestari Djungu Diana A. Wuri Diana A. Wuri Diana Agustiani Wuri Diana Miranti Rihi Dodi Darmakusuma Dondin Sajuthi Eky Melyani Sanu Elisabet Tangkonda Erni Ferawati Kadja Ewaldus Wera Filphin Adolfin Amalo Foeh, Nancy D. F. K. Frans Umbu Datta Fresensi Anggraini Date Meze Gaina, Cynthia Dewi Gomez, Marito Britos Gregorius Ronaldo Mau Kuru Harrold Subu Taopan Hilarius Langobelen Imanuel Benu J F BaleTherik Jayusman Arsiyanti Joesoef Jems Graham Bell Jois M. Jacob, Jois M. Julianty Almet Juni Claudia Dami Kallau, Novalino Harold Geoffrey Karolina Trifonia Diaz Larry Richard Wellem Toha Loe, Fhady R. Loe, Fhady Risckhy Lucyan Maria Azi Owa Milo Maha, Inggrid Trinidad Malelak, Marlin Cindy Claudya Mandala, Graziela Angelicha Manurung, Imelda F.E Manuwolu, Lucy Filipini Maria Aega Gelolodo Maria Geong Maria Kikementina Junersi Marianus Triyanto Sado Mario H.Cantona Mbula, Viktoriano Kingking Dalman Meity Marviana Laut Meity Merviana Laut Michael Haryadi Wibowo Murni, Theresia F. I. M. D. Nadya Daramuli Kale Nahak, Handrianus K. Nalley, Wilmientje Marlene-Mesang Nathasya Pelt Ndaong, Nemay A. Nina Inocensia Welndy Nobrihas, Risty Suryani Nubatonis, Marthen L.O. Olivia Maria Ujan Orolaleng, Katarina Keleka Paulina Jolanda Naif Priska Clayu Apelabi Putra Nugroho, Mega Perkasa Rahmat Setya Adji Rahmat Setya Adji Rizaldo Mariano Ludji Romula A Jemadi Salestin, Ledy Chehfzy Sanam, Viona Mariana Dewi Sera Marbella Christin Langgar Simarmata, Yohanes T. R. M. R. Sitompul, Yeremia Y. Sitompul, Yeremia Yobelanno Soge, Bergitha Tarsisius Considus Tophianong Tarsisius Considus Tophianong Tarsisius Considus Tophianong Tarsisius Considus Tophianong, Tarsisius Considus Telupere, Franky M. S. Theresia Setia Palus Toha, Larry R. W. Toha, Larry Richard Wellem Tophianong, Tarsisius C Tri Utami Tri Utami Tri Utami Tri Utami Tri Utami Tri Utami Weraman, Pius Widi, Antin Y. N. Widi, Antin Y.N Widya Asmara Wilmientje Marlene Mesang Nalley Winda Atika Tosi Wuri, Diana Wuri, Diana A. Yeremia Y. Sitompul Yohanes Naileta Koli Yohanes Simarmata Yohanes T. R. M. R Simarmata Yohanes T. R. M. R. Simarmata Yohanes T.R.M.R Simarmata Yohanes TRMR Si Yohanes TRMR Simarmata Yohanes TRMR Simarmata