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Application of Lactic Acid Bacteria to Produce Bioactive Compounds from Tofu Waste Using Pineapple Bromelain Enzyme Lukman, Zulfiqar; Andrianto, Dimas; Meryandini, Anja
HAYATI Journal of Biosciences Vol. 33 No. 2 (2026): March 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.2.445-454

Abstract

Tofu is one of the main human foodstuffs and has become an alternative substitute for animal protein. Tofu contains relatively high protein, and active compounds, but the waste produced during the tofu processing process is very abundant, namely 3.5 million tons per year in Indonesia. The production of active compounds from tofu waste can use bromelain enzymes. The bromelain enzyme in this study was produced from the fermentation of pineapple leaf waste using Pediococcus pentosaceus E7. The production of active compounds from tofu waste has not been widely reported, so this study aimed to produce active compounds from tofu waste. This study began with bromelain production, measurement of bromelain activity, bromelain precipitation, peptide production from tofu waste, Liquid Chromatography-Mass Spectrometry (LC-MS) analysis, and antibacterial testing of active compounds derived from tofu waste degradation. The results showed that the peptide content of tofu liquid waste degraded using the bromelain enzyme was 41.01±0.06 mg/mL. The LCMS results showed that 18 bioactive compounds were contained in the degradation products of tofu liquid waste. The active compounds derived from the degradation of tofu waste are capable of inhibiting the growth of Salmonella Typhimurium ATCC 14028, yielding a clear zone index of 2.53±0.12.
Antibacterial Activity of Hermetia illucens (Black Soldier Fly) Maggot Extract Against Salmonella typhi Andrianto, Dimas; Hasim; Muhammad, Zulfikar; Dwicesaria, Maheswari Alfira
Current Biochemistry Vol. 12 No. 2 (2025)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.12.2.1

Abstract

Typhus is an acute fever caused by Salmonella typhi bacterial infection. This research aims to test the efficacy of BSF maggot extract as an antibacterial against Escherichia coli and Salmonella typhi. The stages in this research are the preparation of maggot samples, determination of water content of samples, extraction of maggot BSF, screening of antibacterial activity of maggot BSF against Escherichia coli and Salmonella typhi, fractionation from the best results of antibacterial activity of maggot extract BSF and the test of antibacterial activity of maggot BSF fraction against Escherichia coli and Salmonella typhi, fractionation of the best results of antibacterial activity of maggot extract BSF and the test of antibacterial activity of maggot BSF fraction against Escherichia coli and Salmonella typhi. Extraction was carried out by a multi-level maceration method using acetone, methanol and water solvents, then the extract was tested for antibacterial activity against Salmonella typhi using the disc diffusion method with the best results, acetone extract with an inhibition zone of 8.13 mm ± 0.153. Then, acetone extract was fractionated according to its polarity, namely n-hexane, dichloromethane, ethyl acetate, and water. The results of the fractionation were tested for antibacterial activity against Escherichia coli and Salmonella typhi using the disc method with the best fraction was the n-hexane fraction, at a concentration of 2500 ppm with inhibition zones 8.37 mm ± 0.32 (Escherichia coli) and 9.00 mm ± 0.00 (Salmonella typhi)
Pengelolaan Ekosistem Mangrove untuk Mendukung Keberlanjutan Pesisir: Peran Blue Carbon dalam Mitigasi Perubahan Iklim : Penelitian Dimas Andrianto; Jihad Waffiqa Rahmah; Rizki Amelia Sasqia Putri; Eni Kamal; Widya Prarikeslan; Abdul Razak; Mhd. Ridha
Jurnal Pengabdian Masyarakat dan Riset Pendidikan Vol. 4 No. 2 (2025): Jurnal Pengabdian Masyarakat dan Riset Pendidikan Volume 4 Nomor 2 (October 202
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jerkin.v4i2.3638

Abstract

Penelitian ini bertujuan menganalisis perkembangan dan arah penelitian mengenai pengelolaan ekosistem mangrove dalam mendukung keberlanjutan wilayah pesisir selama periode 2019–2025. Pendekatan yang digunakan adalah Systematic Literature Review (SLR) berdasarkan pedoman PRISMA 2020. Sebanyak 15 artikel jurnal ilmiah nasional dan internasional diseleksi dari basis data Scopus, ScienceDirect, SpringerLink, dan Google Scholar. Hasil analisis menunjukkan bahwa tema penelitian mengalami pergeseran signifikan dari konservasi struktural menuju integrasi antara ekologi, sosial-ekonomi, dan kebijakan. Lima tema utama yang diidentifikasi adalah: (1) ekologi dan blue carbon; (2) restorasi dan rehabilitasi; (3) sosial-ekonomi dan kelembagaan; (4) kebijakan dan tata kelola; serta (5) teknologi dan monitoring. Pengelolaan berbasis masyarakat, inovasi pemantauan digital, dan mekanisme pasar karbon biru muncul sebagai pendekatan strategis yang efektif. Namun demikian, ditemukan sejumlah kesenjangan penelitian, antara lain kurangnya studi longitudinal, minimnya data wilayah timur Indonesia, serta lemahnya integrasi kebijakan antar-lembaga. Oleh karena itu, riset ke depan disarankan untuk menggabungkan pendekatan socio-ecological systems dengan penguatan tata kelola dan teknologi partisipatif agar mangrove dapat menjadi fondasi keberlanjutan pesisir dan mitigasi perubahan iklim nasional.
Uji Penghambatan α-Glukosidase dari Kombinasi Ekstrak Kunyit, Teh Hitam dan Jahe: The α-Glucosidase Inhibition Test from a Combination of Turmeric Extract, Black Tea, and Ginger Akhmad Endang Zainal Hasan; Dimas Andrianto; Rara Annisaur Rosyidah
JURNAL AGROINDUSTRI HALAL Vol. 8 No. 1 (2022): Jurnal Agroindustri Halal 8(1)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/jah.v8i1.5608

Abstract

Traditional medicines that can be used as α-glucosidase inhibitors are turmeric (Curcuma longa L.), black tea (Camellia sinensi L), and ginger (Zingiber officinale). It is necessary to research the use of a combination of tea, turmeric, and ginger in inhibiting the α -glucosidase enzyme. This study aims to determine the optimal combination of extracts of turmeric, black tea, and ginger in inhibiting the α-glucosidase enzyme. The research carried out was the measurement of IC50 values for the inhibition of α-glucosidase of each extract, and the inhibition of the enzyme α-glucosidase in combination. The results of the test of ethanol extract of turmeric, ginger and black tea water extract obtained the inhibition value of α-glucosidase with IC50 values of 9.48±0.05 g/mL, 66.64±0.44 g/mL and 9.52±0.25, respectively. F7 is a combination of turmeric, ginger and black tea which produces the highest α-glucosidase inhibition, which is 67.86±0.93%.
Identifikasi Senyawa Metabolit Kopi Robusta (Coffea canephora) dan Kandungan Kafein Kopi Robusta Akibat Fermentasi Bakteri Asam Laktat Lactobacillus plantarum Menggunakan UPLC-QTOF-MS/MS Dimas Andrianto; Dimas Andrianto; R. Haryo Bimo Setiarto
Jurnal Penelitian Pendidikan IPA Vol 11 No 1 (2025): January
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i1.9953

Abstract

Robusta coffee (Coffea canephora) is one type of plant that has long existed in Indonesia and has high economic value. Robusta coffee beans have the potential to be developed to reduce caffeine levels by treating them with lactic acid bacteria. This study aims to determine the metabolite profile of Robusta coffee from several samples treated with Lactobacilus plantarum bacteria. Samples were taken from the West Java location at the Patani Coffee Plantation. Sample extraction was carried out using the Ultrasonic Assisted Extraction (UAE) method with 96% ethanol. Metabolite content analysis was carried out using UPLC-QTOF-MS/MS with a C18 stationary phase column (Okta Decyl Silica), a mixture of formic acid/water 0.1/99.9 (v/v) and formic acid/acetonitrile 0.1/99.9 (v/v) mobile phases. The results of the analysis were interpreted using Masslynx software. The results showed that the caffeine content in coffee not fermented with Lactobacillus plantarum was 2.40%, coffee fermented with L. plantarum bacteria for 6 hours had an abundance% of 1.76%, and coffee fermented with L. plantarum bacteria for 24 hours had an abundance% of 2.38%. LC-MS/MS analysis identified compounds 35,26, and 25 in three Robusta coffee samples. These results were not significantly different from the fermentation of Lactobacillus plantarum in Robusta coffee beans
Preliminary Screening of Proteinaceous and Glycerol-Related Compounds in Commercial Cigarette Filters: Implications For Halal Critical Point Assessment Sumarlin, La Ode; Andrianto, Dimas; Nugraha, Achmad Tjachja; Amilia, Nurul; Megantari, Vetty
Elkawnie Vol. 12 No. 1 (2026)
Publisher : Faculty of Science and Technology Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v12i1.32043

Abstract

Abstract: Cigarette filters are generally regarded as inert components composed primarily of cellulose acetate fibers and plasticizers such as triacetin. However, limited independent analytical studies have investigated the presence of additional compounds that may be incorporated during manufacturing. From a halal science perspective, the presence of proteinaceous materials or glycerol-related compounds may represent a potential halal critical point because their permissibility depends on their biological origin and processing method. This study conducted a preliminary screening of proteinaceous and glycerol-related compounds in several commercial cigarette filters using chromatographic and spectrometric analytical approaches. In addition, a hemoglobin-equivalent colorimetric assay was applied as an initial indicator of protein-like substances. The results indicated the presence of glycerol-related compounds consistent with commonly used plasticizers and humectants in cigarette filter manufacturing. The preliminary screening also suggested the presence of proteinaceous signals in certain samples, although the analytical approach used in this study does not allow for the confirmation of the species origin. These findings highlight the need for more comprehensive analytical verification, including advanced proteomic or DNA-based techniques, to determine the source of such compounds. The study underscores the importance of transparency in material sourcing and manufacturing processes for consumer products that may involve animal-derived substances. Furthermore, this research contributes to the development of analytical frameworks for identifying potential halal critical points in non-food consumer products such as tobacco-related materials. Abstrak: Filter rokok umumnya dianggap sebagai komponen inert yang terutama tersusun dari serat selulosa asetat dan plasticizer seperti triacetin. Namun demikian, kajian analitik independen yang meneliti keberadaan senyawa tambahan yang mungkin dimasukkan selama proses manufaktur masih sangat terbatas. Berdasarkan perspektif sains halal, keberadaan bahan bersifat protein (proteinaceous materials) atau senyawa yang berkaitan dengan gliserol berpotensi menjadi titik kritis halal, karena kehalalannya bergantung pada asal biologis serta metode pengolahannya. Penelitian ini melakukan skrining awal terhadap keberadaan senyawa proteinaceous dan senyawa terkait gliserol pada beberapa filter rokok komersial dengan menggunakan pendekatan analisis kromatografi dan spektrometri. Selain itu, uji kolorimetri hemoglobin-ekuivalen digunakan sebagai indikator awal untuk mendeteksi keberadaan zat yang menyerupai protein. Hasil penelitian menunjukkan adanya senyawa terkait gliserol yang konsisten dengan penggunaan plastisizer dan humektan yang umum digunakan dalam pembuatan filter rokok. Skrining awal juga menunjukkan adanya sinyal yang mengindikasikan keberadaan senyawa proteinaceous pada beberapa sampel, meskipun pendekatan analitik yang digunakan dalam penelitian ini belum memungkinkan konfirmasi asal spesiesnya. Temuan ini menegaskan perlunya verifikasi analitik yang lebih komprehensif, termasuk penggunaan teknik proteomik tingkat lanjut atau metode berbasis DNA, untuk menentukan sumber senyawa tersebut secara lebih pasti. Penelitian ini juga menekankan pentingnya transparansi dalam sumber bahan baku dan proses manufaktur pada produk konsumen yang berpotensi melibatkan bahan turunan hewani. Selain itu, penelitian ini berkontribusi dalam pengembangan kerangka analitik untuk mengidentifikasi potensi titik kritis halal pada produk konsumen nonpangan, seperti material yang berkaitan dengan produk tembakau.
Co-Authors Abdjad Asih Nawangsih Abdul Aziz Abdul Razak Abdurrahman Zaky Achmad Tjachja Nugraha Achyar, Catellia Auliany Agus Setiyono Ahmad Faqih Aisyah Sahara Akhmad Endang Zainal Hasan Al Malik, Ahmad Fauzan Alexandra, Angelya Cintania Amilia, Nurul Amrista Fanzani Kananga Anja Meryandini Antonius Padua Ratu Apipah Aprianti Arya Arendra Assa’addah, Habibah Aulya, Silvy Ayu Tri Nursyarah Bella Fatima Dora Zaelani Dadan Rohdiana Daeli, Gavriel Allenfar Danty Oktiana Prastiwi Dara Anggun Vachyra Dea Khalissa Anidya Destiandani, Khansa Dewi Eriyanti Dewi Kurniasih Dewi Purnamasari Dimas Andrianto Djarot Sasongko Hami Seno Dwicesaria, Maheswari Alfira Eka Wahyu Purnama Elgiani Yassifa Yulia Nur Insari Emidar Khusnu Eni Kamal Eny Ida Riyanti Eriyanti, Dewi Etik Mardliyati, Etik Eva Harlina Fahmi Hafid Faisal Rahman Falah, Samsul Febrina, Adella Fina Febrianti Firdaus, Dzikri Anfasa Firdaus, Muhamad Fajar Firmansyah, Muhammad Ade Fitrilia, Tiana Galuh Adhiyaksa Ashari Ghifari, Muhammad Nabil Gholam, Gusnia Meilin Gina Oktaviani Sabrina Hardinsyah Hasanah, Ikromatul Hasim Hasim - HASIM DANURI Hasim Hasim Hazim, Naufal Wildan Heddy Julistiono Heddy Julistiono Hudayanti, Martini Husnawati, . I MADE ARTIKA Ike Agustiani Ilham Gilang Cahya Ahmada Imelia Dewi Inawati Inawati Irfan Abdul Aziz Irsal, Riyan Alifbi Putera Istikorini, Yunik Jaka Aryantara Kuntala Jihad Waffiqa Rahmah Kusnaedi, Anggi La Ode Sumarlin LAKSMI AMBARSARI Latifah Kosim Darusman Latifah, Aminahtun Lestari, Ella Deffi Lukman, Zulfiqar Madyastuti, Rini Marfiana, Arila Safa Maria Bintang Mega Safithri Megantari, Vetty Mhd. Ridha Muhamad Alvin Adriyan Muhammad Farhan Muhammad Irfansyah Pradika Muhammad Zulkifli Mutholaah Mutmainnah Agustiawan Umar Nabilatusyifa, Alivianisa Nabilla, Rahmah Najmah, Najmah Nayanda, Najwa Putri Nina Tri Kusumawati Noor Farikhah Haneda Noviyan Darmawan Nurliani Bermawie Nusyarah, Ayu Tri Olive Wisepti Pratama Prasetyo, Dwiandra Hadi Purwanto, Ukhradiya Magharaniq Safira Putri, Oyi Candra Santoso Putri, Salsabila Yasmin Budi Qolbiyah, Syifa Ainul Qomariah Hasanah Qurrotu Ayni R. Haryo Bimo Setiarto Raden Roro Fine Ayu Putri Rafiqi, Akbar Rahmah, Sabrina Firdayani Raisyadikara, Fadila Rara Annisaur Rosyidah Rimbawan Rimbawan Rini Kurniasih, Rini Riska Susila Putri Rizki Amelia Sasqia Putri Rosyidah, Rara Annisaur Sanro Tachibana Sari, Sintia Permata Sigiro, Ria Heni Silvy Aulya Sitanggang, Rianta Faustina Siti Maesunah Gilang Maya Slamet Hamzah Sobari, M Hilmy Rifq Sri Anna Marliyati Sri Mariati Sri Wahyuni Suci Hermita Sulistiyani SULISTIYANI SULISTIYANI Sulistiyani Sulistiyani Syaefudin Syaefudin Syaefudin Syamsul Falah Syarthibi, Ahmad Syifa Khaerani Tukan, Maria Magdalena Nona Motu Ulfah Juniarti Siregar Vina Oktovianti Walidayyin, Sekar Wangi Waras Nurcholis Widya Prarikeslan Wilujeng, Retno Winata, Grace Marlina Yudono, Nur Annisa Windi Yupi Yulianita Arifin Yusriya, Rifqi Zaelani, Bella Fatima Dora Zulfikar Muchammad Zulfikar Muhammad, Zulfikar