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Jamur Non-Dermatofita Pada Kuku Jari Tangan (Finger Nails) Penyebab Onikomikosis Fajar Husen; Nuniek Ina Ratnaningtyas; Nur Aini Hidayah Khasanah; Nilasari Indah Yuniati; Dian Islamiyati
Jurnal Bina Cipta Husada Vol 19 No 1 (2023): Jurnal Bina Cipta Husada
Publisher : STIKes Bina Cipta Husada

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Abstract

Jamur dermatofita merupakan kelompok jamur patogen yang umum dijumpai di negara tropis seperti Indonesia. Jamur ini seringkali menyebabkan infeksi pada kuku (Tine unguium). Selain disebabkan oleh kelompok jamur dermatofita, penyakit kuku yang masuk ke dalam kelompok penyakit mikosis superfisialis, juga dapat disebabkan oleh jamur non dermatofita serta pada kasus yang cukup jarang disebabkan oleh ragi. Akibat infeksi jamur kuku, bagian kuku yang terinfeksi akan menjadi rapuh, mengelupas dan berubah warna. Tujuan penelitian ini adalah untuk mengetahui jenis jamur non-dermatofita yang menyebabkan penyakit mikosis superfisialis pada kuku, khususnya Onikomikosis. Penelitian ini merupakan riset deskriptif, dimana sampel pedagang ayam yang diambil kukunya kemudian dikultur pada media biakan potato dextrose agar (PDA), kemudian hasil isolasi dan re-kultur diamati dan diidentifikasi secara makromorfologi dan mikromorfologi. Pengamatan mikromorfologi dilakukan dengan menggunakan mikroskop cahaya binokuler dengan perbesaran 400x. Pengamatan makromorfologi meliputi bentuk koloni, tepi koloni, warna koloni, dan permukaan koloni. Data hasil pengamatan kemudian dianalisis secara deskriptif sesuai hasil observasi mikroskopis dan makroskopis. Hasil penelitian menunjukkan bahwa terdapat dua jenis jamur non-dermatofita yang ditemukan pada sampel kuku pedagang ayam potong di Desa Mandiraja Wetan, Kabupaten Banjarnegara, yaitu Apergillus flavus dan Aspergillus niger.
Anti-inflammatory and immunosuppressant activity of Coprinus comatus ethanol extract in carrageenan-induced rats (Rattus norvegicus) Nuniek Ina Ratnaningtyas; Fajar Husen; Hernayanti Hernayanti; Nuraeni Ekowati; Bambang Heru Budianto
Molekul Vol 17 No 3 (2022)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2022.17.3.5815

Abstract

oprinus comatus (O.F. Mull.) is an edible mushroom that is used as an anti-inflammatory agent. Therefore, this study aims to determine the effect of inflammation treatment on symptoms alleviating, function maintenance, and inhibiting the process of tissue damage due to an increase in free radicals using drug formulations with high antioxidant compounds. This is a true experiment conducted using a Completely Randomized Design (CRD) with a post-test and a control group. The rats were divided into 6 categories, which include 1 healthy and 5 groups induced with 1% carrageenan. Out of the treatment groups, 3 were treated with ethanol extract of C. comatus fruiting body at doses of 250 (T1), 500 (T2), and 750 mg/kg BW (T3), 1 received diclofenac sodium (PC) and the other as a negative control (NC), were given extract for 14 days and induced with 0.5 mL carrageenan in paw of rats at day 15. The qualitative identification showed the extracts contains flavonoid, polyphenol, alkaloid, triterpenoid, steroids and saponins, and GC-MS analysis showed 10 putative metabolites compound. T2 group significantly decreased the levels of IL-1β (70.63%), IgE (59.04%), total leukocyte count (31.24%), plantar thickness (12.5%), edema volume (33.3%), and increased endothelial NO levels (48.2%).
Detoxification of Cadmium on Albino Rats (Rattus norvegicus) with Natural Chelator of Fruiting Body Extract of Ganoderma lucidum Hameda Dhaka Kusuma Taufiq; Hernayanti Hernayanti; Nuniek Ina Ratnaningtyas
Molekul Vol 18 No 1 (2023)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2023.18.1.5844

Abstract

Cadmium is a heavy metal pollutant sourced from various industries and toxic to the kidneys. Cadmium exposure can be used natural chelator of ethanol extract of the fruiting body of Ganoderma lucidum. The aim of the study was to determine the effect and effective dose of the ethanolic extract of the fruiting body of G. lucidum on reducing the toxicity effect of cadmium in male albino rats (Rattus norvegicus) Wistar strain. The research design was experimental research, which consisted of 5 treatment levels. The research parameters were blood cadmium, β2-microglobulin, malondialdehyde, and superoxide dismutase levels. The ethanolic extract of fruiting body of G. lucidum was tested by gas chromatography-mass spectrometry (GCMS) to determine linalool as active compounds. The results of each parameter had a significant effect, which decreased blood cadmium, β2M and MDA levels, and increased SOD level that linear according to the addition of the dose of ethanol extract of the fruiting body of G. lucidum. Blood cadmium levels with β2M and MDA levels had a positive correlation, while blood cadmium levels with SOD levels had a negative correlation. The dominant compound detected was linalool which has potential as a radical scavenger. The dose of 750 mg.kgBW-1 is the effective dose of the ethanolic extract of fruiting body of G. lucidum based on a decrease in blood cadmium levels (54.10%), β2M (63.94%) and MDA (20.31%), as well as an increase in SOD levels (14.20%) compared to sick control.
POTENSI AKTIVITAS ANTIBAKTERI EKSTRAK Moringa oleifera (Lamk.) TERHADAP BAKTERI ESCHERICHIA COLI SECARA IN SILICO DAN IN VITRO Dian Islamiyati; Fajar Husen; Nuniek Ina Ratnaningtyas
Jurnal Bina Cipta Husada Vol 19 No 2 (2023): Jurnal Bina Cipta Husada
Publisher : STIKes Bina Cipta Husada

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Moringa oleifera (Lamk.) atau daun kelor, adalah salah satu jenis tumbuhan khas di Asia Tenggara, khususnya di Indonesia. Daun kelor dikenal sebagai tumbuhan dengan kandungan senyawa bioaktif yang multiaktivitas. Senyawa flavonoid, quercetin, polifenol, tannin, dan saponin pada daun kelor memiliki aktivitas sebagai anti peradangan, antidiabetes, dan antibakteri. Potensi ekstrak daun kelor sebagai antibakteri perlu diuji coba secara in silico dan in vitro untuk mengetahui kecocokan dari senyawa bioaktif spesifik yang memiliki potensi aktivitas antibakteri tertinggi. Metode penelitian ini adalah deskriptif analitik. Percobaan in silico dilakukan dengan menggunakan program software computer untuk senyawa quercetin, dengan kontrol positif adalah antibiotik chloramphenicol. Sementara percobaan in vitro antibakteri ekstrak dilakukan dengan menggunakan metode sumuran. Hasil pengujian in silico menunjukkan bahwa ekstrak daun kelor memperlihatkan potensi yang baik sebagai antibakteri khusunya E. coli, namun aktivitasnya lebih kecil dibandingkan dengan antibiotik chloramphenicol. Sementara hasil investigasi dan observasi in vitro menunjukkan hasil yang sama, dimana chloramphenicol menunjukkan hasil inhibisi terbaik dengan zona hambat 54 mm, sementara ekstrak M. oleifera memiliki zona hambat 24 mm.
Antioxidant Potential of Ethanol and Ethyl Acetat Extract of Ganoderma sp. Mycelium Nuniek Ina Ratnaningtyas; Purnomowati Purnomowati; Endang Sri Purwati; Aisyah Tri Septiana; Nuraeni Ekowati; Adi Supriyadi
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.11512

Abstract

Ganoderma sp. Banyumas 1 isolate that reffered as Ganoderma sp. is a new discovered isolate from Banyumas, Central Java, Indonesia expected to have a potential properties of antioxidant of medicinal mushroom. This study aimed to determine the antioxidant potential and the appropriate solvent for it’s extracting from Ganoderma sp. This research result showed that ethyl acetate was able to extract as many as 15.57%, while etanol was only able to extract 3.87% active compounds from dried 28 days old Ganoderma sp. mycelium cultivated in the Mushroom Complete Medium (MCM). Extract of ethyl acetate (non-polar) extraction of mycelium of Ganoderma sp. had a potential character as an antioxidant source and performed a better result than from ethanolic (polar) extraction as shown in the IC50 value. Extract from ethyl acetate extraction had an average IC50 value smaller than  from ethanolic extract (581.80 1285.67). Extract from ethyl acetate extraction resulted in a higher amount of phenol than that ethanolic extract 29.23 57.67. Inhibition percentage of both extracts at 65% was known to occur at concentration of 1000 ppm for ethyl acetate extract and 2000 ppm for ethanolic extract. An important finding was that ethyl acetate can be used as appropriate solvent for extracting antioxidant compound better than ethanolic. In conclusion, the mycelium extract of Ganoderma sp. extracted with ethyl acetate and ethanol as solvent is potential to be used as a source of natural antioxidants. This research result has benefit in developing potency of local resources as herbal resources.
Effects of Ganoderma lucidum Extract on Diabetic Rats Nuniek Ina Ratnaningtyas; Hernayanti Hernayanti; Suci Andarwanti; Nuraeni Ekowati; Endang Sri Purwanti; Dalia Sukmawati
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i3.15356

Abstract

Diabetes mellitus (DM) is a metabolic syndrome which occurs when insulin is insufficiently produced or insulin cannot well serve its function. Diabetes is marked with increase in blood glucose level followed by increase in glycosylated hemoglobin level and decrease in insulin level. This research aims to examining the effect of Ganoderma lucidum extract on the blood glucose, insulin and glycosylated hemoglobin (HbA1c) level of diabetic white rat and determining the most effective dose of extract to be a diabetic agent. This research was experimentally conducted by employing Completely Randomized Design (CRD) with 6 treatments and 4 repetitions. The treatment groups consisted of healthy rats group (K1), rats with diabetes as negative control (K2), diabetic rats with the administration of metformin 45 mg/kg BW as the comparison (K3) and diabetic rats with the administration of G. lucidum extract with dose of 250, 500 and 1000 mg /kg BW (K4, K5 and K6 respectively). Blood glucose level examination was conducted after the alloxan induction with single dose of 125 mg/kg BW by intraperitoneal injection. The results show that mushroom G. lucidum extract administration with dose of 1000 mg/kg BW (K6) is the best dose to be an anti-diabetic agent. The benefit of the research is developing anti-diabetic agent from herbal resources.
Antidiabetic Potentials of Button Mushroom (Agaricus bisporus) on Alloxan-Induced Diabetic Rats Nuraeni Ekowati; Nilasari Indah Yuniati; Hernayanti Hernayanti; Nuniek Ina Ratnaningtyas
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i3.17126

Abstract

Button mushrooms (Agaricus bisporus) is an edible mushroom that is most widely cultivated in the world. It contains bioactive compounds that might provide beneficial effects on diabetes mellitus patient. The study aimed to determine the effects of A. bisporus administration on the blood glucose, and malondyaldehyd (MDA) levels as well as superoxide dismutase (SOD) activity of alloxan-induced diabetic rats. This study was also conducted to determine the secondary metabolites produced by A. bisporus. The method used was experimental methods with Completely Randomized Design. A. bisporus extract at the doses of 250, 500 and 750 mg/kg body weight (BW) per day were orally applied to alloxan-induced diabetic rats for a period of 14 days after the rats became diabetes. The results showed that the extract of A. bisporus could decrease blood glucose, and MDA levels as well as increase SOD activity (p 0.05). A. bisporus extract 500 mg/kg BW is the most effective dose to be used. Based on Thin Layer Chromatography (TLC) test, it was known that secondary metabolites produced by A. bisporus are flavonoids, alkaloids, terpenoids and saponins. A. bisporus has potential as an antidiabetic through the ability to decrease blood glucose, and MDA levels, as well as increase SOD activity in diabetic rats. This research is able to provide information about the antidiabetic potential of A. bisporus extract so that it can be used as an alternative natural antidiabetic agent and can be applied in the community with ease and in a more controlled industrial scale.
Soybean Selection Against Cercospora Leaf Blight Disease Caused By Cercospora kikuchii Based on Anatomical Resistance Fajar Husen; Nuniek Ina Ratnaningtyas; Siti Samiyarsih; Juni Safitri Muljowati; Nur Fitrianto
Biosaintifika: Journal of Biology & Biology Education Vol 14, No 1 (2022): April 2022
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v14i1.34865

Abstract

Soybean (Glycine max L. Merr.) is the third food crop commodity after rice and maize in Indonesia. This plant is also known as the most important source of vegetable protein, which is relatively inexpensive, but a decrease in soybean productivity can occur due to infection with disease-causing pathogens, one of is Cercospora kikuchii which causes Cercospora leaf blight (CLB). The research objectives were to determine the anatomical resistance and disease severity of soybean cultivars against CLB. The method was an experiment with a completely randomized design (CRD) factorial pattern; factor 1 being soybean cultivars (Dering, Slamet, Grobogan, Wilis) and factor 2, namely pathogen inoculation (0 conidiospores/mL and 105 conidiospores/mL). Anatomical method preparations using paraffin, staining with 1% safranin. Disease criteria are based on the council of scientific and industrial research (CSIR) assessment method. Data were analysis used analysis of variance (p0.05) and the least significance difference (LSD). The results showed that Dering and Slamet cultivars had the largest cuticle, epidermis, and palisade ratios and the smallest stomata length and width with the largest number of stomata and trichomes compared to Grobogan and Wilis. The disease severity (DS) of the cultivars Dering 14.6%, Slamet 24.64%, Grobogan 24.80% (classified as a resistant with low infection), while Wilis cultivar was 31.08% as a moderately susceptible cultivar with moderate infection. The novelty of soybean cultivar selection against CLB is important and its effectiveness for increasing soybean productivity. Dering, Slamet and Grobogan are likely to be further developed with their resistance to CLB disease.
Antioxidant Activities and Properties of Coprinus comatus Mushroom Both Mycelium and Fruiting Body Extracts In Streptozotocin-Induced Hyperglycemic Rats Model Nuniek Ina Ratnaningtyas; Hernayanti Hernayanti; Nuraeni Ekowati; Fajar Husen; Isnaini Maulida; Riama Kustianingrum; Vika Vidiyanti
Biosaintifika: Journal of Biology & Biology Education Vol 14, No 1 (2022): April 2022
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v14i1.34244

Abstract

Coprinus comatus, well-known as Shaggy Ink Cap mushroom, is potential herbal medicine. Synthetic medicines sometimes cause side effects; therefore, it is necessary to innovate with herbal medications with minimal side effects. The study evaluated in vitro and in vivo treatments to evaluate the antioxidant effect and activity of C. comatus. The in vivo treatment was conducted using six groups of Wistar rats (n = 24). Group 1 healthy control (HC), groups 2–6 received 45 mg/Kg BW of streptozotocin once, group 2 just streptozotocin-induced (NC), group 3 was given 45 mg/kg BW of metformin (PC), groups 4–6 were given 250 (T1), 500 (T2), and 750 mg (T3) of C. comatus extract for 14 days, and the in vitro was conducted using an antioxidant oxidant assay. Data were analyzed using analysis of variance and Duncan's multiple range tests. Based on qualitative analysis, C. comatus mycelium extract contained polyphenol, flavonoids, terpenoids, and fruiting body extract had flavonoids, alkaloids, and saponins. The in vitro analysis showed that the mycelium extract had an antioxidant activity by inhibiting free radicals up to 58.51% with an IC50 value of 72.77 mg/L. The in vivo treatment using C. comatus fruiting body extract showed that it could increase the endogenous antioxidant levels of GPx, SOD, catalase and reduce MDA levels (p 0.05). The most effective dose of C. comatus extract is 500 mg. This research has shown the potential of mycelium and C. comatus fruitbody extract as an antioxidant supplement in a diabetic rat model.
Tinjauan Biosorpsi Logam Berat Pb dan Cd Oleh Jamur Makro Noverita Noverita; Nuniek Ina Ratnaningtyas; Endang Sukara; Nuraeni Ekowati; Sri Lestari
Al-Kauniyah: Jurnal Biologi Vol 16, No 2 (2023): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v16i2.24569

Abstract

AbstrakKeberadaan logam berat perlu ditanggulangi, salah satunya dengan cara biosorpsi. Biosorpsi merupakan salah satu metode remediasi yang paling tepat digunakan dalam menanggulangi pencemaran logam berat. Proses secara efisien dapat menyerap logam berat terlarut bahkan logam berat yang ada dalam larutan kompleks yang sangat encer. Jamur makro merupakan contoh biosorben yang dapat digunakan dalam biosorpsi. Pemanfaatan jamur makro sebagai biosorben sangat tepat dilakukan karena keanekaragaman jenisnya tinggi, cepat tumbuh dengan kemampuan metabolisme yang beragam pada berbagai senyawa organik dan anorganik, mudah didapatkan, lebih aman karena tidak menyebabkan korosi atau menghasilkan bahan berbahaya, teknologinya sederhana, perawatannya mudah dan produk akhir dapat didaur ulang, serta dapat mengakumulasi logam berat dengan kosentrasi tinggi. Jenis-jenis jamur makro yang sudah digunakan dalam biosorpsi logam berat di beberapa negara antara lain Agaricus bisporus, Auricularia polytricha, Calocybe indica, Ganoderma carnosum, Flammulina velutipes, Fomes fasciatus, dan Volvariella volvacea. Sementara itu, penelitian penggunakan jamur makro di Indonesia dalam biosorpsi logam berat sangat terbatas yakni pada jenis Phanerochaete chrysosporium, Omphalina sp., dan Pholiota sp. Mengingat tingginya keanekaragaman jamur makro di Indonesia, maka potensi biosorpsi logam berat khususnya Pb dan Cd oleh jamur makro sangat berpeluang untuk diteleti lebih lanjut.AbstractHeavy metals can accumulate in seawater, sediments and in the marine biota that live in them, eventually entering the food chain which is very dangerous to health. The presence of these heavy metals needs to be overcome, one of which is by means of biosorption. Biosorption is one of the most appropriate remediation methods used in tackling heavy metal pollution. The biosorption process can efficiently absorb dissolved heavy metals and even heavy metals present in very dilute complex solutions. Macro fungi are examples of biosorbents that can be used in biosorption. Utilization of macro fungi as biosorbents is very appropriate because of the high diversity of species, fast growing with diverse metabolic abilities on various organic and inorganic compounds, easy to obtain, strong morphology, safer because they do not cause corrosion or produce harmful materials, simple technology, easy maintenance and the final product can be recycled, can accumulate heavy metals with high concentrations. The types of macro fungi that have been used in the biosorption of heavy metals in several countries include Agaricus bisporus, Auricularia polytricha, Calocybe indica, Ganoderma carnosum, Flammulina velutipes, Fomes fasciatus, and Volvarella volvacea. Meanwhile in Indonesia, research on the use of macro fungi in heavy metal biosorption is very limited to the Phanerochaete chrysosporium, Omphalina sp. and Pholiota sp. Considering the high diversity of macro fungi in Indonesia, the potential for biosorption of heavy metals, especially Pb and Cd by macro fungi, is very likely to be investigated further.
Co-Authors . HERNAYANTI AA Sudharmawan, AA Adi Supriyadi AGUS HERY SUSANTO Agus Irianto Aisyah Tri Septiana Aisyah Tri Septiana Amalia Sofia Maharani Amelia, Aurora Pradipta Andarwanti, Suci angelin marhavyna cristy Anik Laeli Perdanawati Apriyani, Tri Rahayu Ari Asnani Arif Rahman Hikam Aris Mumpuni Ashar, Fadli Atik Retnowati Azhar Faturohman Abidin Azizah, Enur Bahrun Bahrun Bambang Heru Budianto Budiarti, Vio Indah cristy, angelin marhavyna Dalia Sukmawati Daniel Joko Wahyono Dattadewi Purwantini Dhea Rifa Rahmah Edyawati Dian Islamiyati Dian Islamiyati Dini Ryandini Dini Ryandini Dody Novrial, Dody Doso Sarwanto Dyah Fitri Kusharyati Eko Setio Wibowo Ellen Evita Endang Sri Purwanti Endang Sri Purwanti Endang Sri Purwati Endang Sri Purwati Endang Sukara Evita, Ellen Fajar Husen Feryawan Feryawan Feryawan, Feryawan Fitria Dewi Sulistiyono Fitrianto, Nur Fitriya Yuni Wulandari Haris Budi Widodo Herliana, Okti Hernayanti Hernayanti Hernayanti Hernayanti Hernayanti Hernayanti HERNAYANTI HERNAYANTI Hernayanti Hernayanti Hernayanti Hernayanti Irfan Dwiandhono Isnaini Maulida Juni Safitri Muljowati Juni Safitri Muljowati Khasanah, Nur Aini Hidayah Kurniawan Kurniawan Loekas Soesanto Loekas Soesanto Maharani, Amalia Sofia Maria Mardhitama Maharani MU'AFA, RAFID HILMY Muntahanah, Siti Nahdatulia, Yasinta Nawangtantrini, Gita Ningrum, Ema Wahyu Noverita Nur Aini Hidayah Khasanah Nur Aini Hidayah Khasanah Nur Fitrianto Nuraeni Ekowati Nuraeni Ekowati Nuraeni Ekowati Nuraeni Ekowati Nuraeni Ekowati Nuraeni Ekowati Nuraeni Ekowati Nuraeni Ekowati Nuraeni Ekowati Nuraeni Ekowati, Nuraeni Nuraini Hidayah Khasanah Oedjiono P.M. Hendrati Palindung, Lusia Seti Perdanawati, Anik Laeli Purnomowati Purnomowati Purnomowati Purnomowati, Purnomowati Purwanto Bekti Santoso, Purwanto Bekti Ratna Stia Dewi Ratri Noor Hidayah Riama Kustianingrum Rifda Naufalin Rizki Maulida Ross Nurul Rohmah Ryan Firman Syah Salamiah Salamiah Saputra, Wahyu Dwi saraswati, esti Siti Samiyarsih Siti Samiyarsih Siti Samiyarsih Slamet Priyanto Sri Hartini Sri Lestari Sri Lestari Suci Andarwanti Supriyadi, Adi Taufiq, Hameda Dhaka Kusuma Tri Rahayu Apriyani Tyas Retno Wulan Ulfa Fadila Rudatiningtyas Ulfa Fadilla Rudatiningtyas Utami, Nurani Ajeng Tri Vika Vidiyanti Vio Indah Budiarti Wahyu Dwi Saputra Wirakhmi, Ikit Netra Witiyasti Imaningsih Yanita Mutiaraning Viastika Yulia, Ana Yuniati, Nilasari Indah Yunida Triana, Noor Zahra, Jauza Ulya