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<![CDATA[EFEKTIVITAS EKSTRAK KAPANG ENDOFIT ISOLAT BR-S1 (A) TERHADAP BAKTERI Methicillin-Resistant Staphylococcus aureus (MRSA) ]]>
<![CDATA[Kurniawan kurniawan kurniawan]]>;
<![CDATA[Nuniek Ina Ratnaningtyas]]>
<![CDATA[ Meditory : The Journal of Medical Laboratory Vol 6, No 2 (2018): Meditory, volume 6, nomer 2, Desember 2018 ]]>
Publisher : <![CDATA[Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar ]]>
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DOI: 10.33992/m.v6i2.417
<![CDATA[Background Tea parasitic plant (Scurrula oortiana L.) is one of medicinal plants (herba medicina) containing several active compounds but its utilization is still constrained by some problems, so that there needs other approaches by utilizing potential of BR-S1 (A) isolate endophytic fungi which grows in these plants to control MRSA pathogenic bacteria. Objective This research aimed to examine the effectiveness of the four types of BR-S1 (A) endophytic fungi extract from the origin of tea parasites to the growth of MRSA bacteria.Methods The researchers used laboratory experimental method by Kirby Bauer disk diffusion method and the data obtained were analyzed statistically. Result The research result showed that MRSA bacteria had various sensitivity on four types of BR-S1 (A) isolate endophytic fungi; with its highest sensitivity on extract of ethyl acetate and its lowest sensitivity was extract of n-hexane (no inhibition). The difference of sensitivity test results was suspected to be related to active compound content found in ethyl acetate extract in form of flavonoid and tannin class compounds which had been proven to inhibit growth of pathogenic bacteria. Conclusion It can be concluded that the four types of BR-S1 (A) endophytic fungus extract originating from tea parasites, only ethyl acetate extract was the most effective in inhibiting the growth of MRSA bacteria, this is due to the content of two active compounds from the flavonoid and tannin groups.]]>
<![CDATA[Karakterisasi Ganoderma spp. di Kabupaten Banyumas dan Uji Peran Basidiospora dalam Siklus Penyakit Busuk Batang ]]>
<![CDATA[Nuniek Ina Ratnaningtyas]]>;
<![CDATA[Siti Samiyarsih]]>
<![CDATA[ Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 29, No 1 (2012) ]]>
Publisher : <![CDATA[Fakultas Biologi | Universitas Jenderal Soedirman ]]>
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DOI: 10.20884/1.mib.2012.29.1.233
<![CDATA[Ganoderma sp., one of fungi belonged to the family Ganodermataceae, order of Aphyllophorales, class of Basidiomycetes is very widely spread. This fungus is a soil-borne, and has parasitic and saprophytic features which are interesting because of their two contradictory roles namely harmful and beneficial effects. As a plant parasite, Ganoderma is able to cause root and stem rot of tropical perennial crop plantation and forest which cause serious losses. The fungus is well known as white rot fungus which is able to cause a wood rotten by lignin destruction. On the contrary, the fungus has some advantages such as medical potentials. In this study, some collections and characterization of Ganoderma spp. found in Banyumas has been conducted. The technique used for sampling Ganoderma spp. mushroom is a survey with Purposive Random Sampling method. Samples were taken from nature in the area of Banyumas Regency and its natural substrate were noted. Characterization based on the macromorphology and micromorphology was done for grouping and identification. Micromorphology observations were done by slicing and painting samples with plants microtechnique method. The result showed that there were 43 Ganoderma spp isolates, distributed from low to high lands of 100 - 500 m above sea level. The most fungi were found on the high lands, in Baturraden sub district, which included 16 isolates. The interesting finding is the discovery of Ganoderma spp. which attacked flamboyant plant (Delonix regia) in West Purwokerto sub district. Based on micromorphology observations, these fungi form a basidium and basidiospore inside and below as well as the surface of the fruit body. Some species were also found having such phenomenon so it needs further research on the role of basidiospora in the cycle of stem rot disease, especially basidiospora role in the initiation of diseases infection. As the soil infecting mushroom, study of the disease spreading is focused more on the role of the mycelium found in the remaining plants as infecting the material. Basidiopore role in the spread of the disease is rarely studied, but actually basidiospore is a potential source of genetic variation.]]>
<![CDATA[Optimasi Konsentrasi Glukosa dan Waktu Inkubasi Terhadap Bobot β-Glukan Jamur Schizophyllum commune ]]>
<![CDATA[Tri Rahayu Apriyani]]>;
<![CDATA[Nuraeni Ekowati]]>;
<![CDATA[Nuniek Ina Ratnaningtyas]]>
<![CDATA[ BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 4 (2021): BioEksakta ]]>
Publisher : <![CDATA[Fakultas Biologi Universitas Jenderal Soedirman ]]>
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DOI: 10.20884/1.bioe.2021.3.4.4601
<![CDATA[The main purpose of this study was to determine the effect of the interaction between glucose concentration and incubation time on the growth of fungi S. commune and also the weight of β-glucan was used to determine the best glucose concentration and incubation time for the growth of fungi S. commune and β-glucan production. The research was conducted by experimental method of completely randomized factorial design (CRD factorial) with two factors carried out in three repetitions. The treatments provided included variations in glucose concentration (G) with three levels, namely 10 g/L (G1), 20 g/L (G2), and 30 g/L (G3), and variations in incubation time (W) with three levels, namely incubation time of 20 days (I1). , incubation time of 25 days (I2), and incubation time of 30 days (I3). Variables which used in this research independent and dependent variables. The independent variables were glucose concentration and incubation time, while the dependent variables were fungal mycelium growth and β-glucan production. The main parameter observed was β-glucan weight. The supporting parameters were dry biomass weight and the final pH medium. Data analysis was performed by Analysis of Variance (ANOVA) at 95% accuracy levels, followed by Duncan's test (Ducan Multiple Range Test). The results showed that glucose concentration and incubation time were significantly affected to the growth and production of β-glucan fungus S. commune. Glucose concentration of 30 g/L and incubation time of 25 days was the optimum glucose concentration and incubation time for the growth of S. commune fungus, and also glucose concentration of 30 g/L and incubation time of 25 days was the optimum glucose concentration and incubation time for β-glucan production of S. commune.]]>
<![CDATA[Pengaruh pH dan Waktu Inkubasi Berbeda Terhadap Pertumbuhan dan Produksi β-Glukan Schizophyllum commune ]]>
<![CDATA[Amalia Sofia Maharani]]>;
<![CDATA[Nuraeni Ekowati]]>;
<![CDATA[Nuniek Ina Ratnaningtyas]]>
<![CDATA[ BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 1 (2022): Bioeksakta ]]>
Publisher : <![CDATA[Fakultas Biologi Universitas Jenderal Soedirman ]]>
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DOI: 10.20884/1.bioe.2022.4.1.4594
<![CDATA[The main purpose of this study are to determine the optimum value of pH and incubation time on S. commune's growth, and also the optimum value of pH and incubation time on β-glucan production of S. commune. The research was conducted by experimental method of completely randomized factorial design (CRD factorial) with two factors carried out in three repetitions. The treatments provided included variations in pH (P) with three levels, namely pH 5 (P1), pH 6 (P2), and pH 7 (P3), and variations in incubation time (W) with three levels, namely incubation time of 20 days (W1). , incubation time of 25 days (W2), and incubation time of 30 days (W3). Variables which used in this research independent and dependent variables. The independent variables were pH and incubation time, while the dependent variables were fungal mycelium growth and β-glucan production. The main parameter observed was β-glucan weight. The supporting parameters were dry biomass weight and the final pH medium. Data analysis was performed by Analysis of Variance (ANOVA) at 95% accuracy levels, followed by Duncan's test (Ducan Multiple Range Test). The results showed that pH and incubation time were significantly affected to the growth and production of β-glucan fungus S. commune. The value of pH 6 and incubation time of 25 days was the optimum pH and incubation time for the growth of S. commune fungus, and also pH 5 and incubation time of 25 days was the optimum pH and incubation time for β-glucan production of S. commune.]]>
<![CDATA[HUBUNGAN DAN PROFIL TEKANAN DARAH DENGAN PENINGKATAN KADAR GLUKOSA DARAH PEDAGANG DI DESA MANDIRAJA WETAN ]]>
<![CDATA[Fajar Husen]]>;
<![CDATA[Nuniek Ina Ratnaningtyas]]>
<![CDATA[ Jurnal Penelitian dan Pengabdian Kepada Masyarakat UNSIQ Vol 9 No 3 (2022): September ]]>
Publisher : <![CDATA[Lembaga Penelitian, Penerbitan dan Pengabdian Masyarakat (LP3M) UNSIQ ]]>
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DOI: 10.32699/ppkm.v9i3.3163
<![CDATA[Tingkat kesehatan masyarakat dewasa ini menunjukkan tern penurunan dengan berbagai penyebab, seperti peningkatan radikal bebas dan pola konsumsi nutrisi yang menurun. Hipertensi dan diabetes masih menjadi permasalahan yang paling umum pada masyarakat pedesaan. Pentingnya monitoring dan pengecekan berkala menjadi salah satu cara preventif untuk mencegah berkembangnya penyakit hipertensi dan diabetes.Tujuan studi ini adalah untuk mengevaluasi dan memonitoring serta mengevaluasi hubungan kejadian hipertensi dengan diabetes serta kaitannya dengan penngkatan usia pada pedagang. Penelitian ini dilakukan dengan menggunakan 35 sampel warga Desa Mandiraja Wetan yang berprofesi sebagai pedagang, pengambilan sampel yaitu purposive random sampling, dengan parameter tekanan darah, glukosa darah dan parameter pendukung yaitu usia. Data dianalisis dengan uji korelasi bivariat (pearson correlation) dengan nilai signifikansi p < 0.05, menggunakan software SPSS ver.26.0. Hasil penelitian menunjukkan terdapat hubungan antara kejadian hipertensi dengan peningkatan kadar glukosa darah serta usia (p < 0.05). Rata-rata tekanan darah tertinggi 150.71/88.31 mm/Hg, kadar glukosa darah tertinggi pada pria 193 mg/dL, wanita 305 mg/dL dengan rata-rata usia pria 57.80 tahun, dan wanita 51.65 tahun.]]>
<![CDATA[Peningkatan Kadar Kolesterol dan Usia Pada Ibu Rumah Tangga ]]>
<![CDATA[Fajar Husen]]>;
<![CDATA[Nuniek Ina Ratnaningtyas]]>;
<![CDATA[Nur Aini Hidayah Khasanah]]>;
<![CDATA[Nilasari Indah Yuniati]]>
<![CDATA[ Jurnal Ilmiah Kesehatan Sandi Husada Vol 11 No 2 (2022): Jurnal Ilmiah Kesehatan Sandi Husada ]]>
Publisher : <![CDATA[Lembaga Penelitian dan Pengabdian Masyarakat Akademi Keperawatan Sandi Karsa (Merger) Politeknik Sandi Karsa ]]>
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DOI: 10.35816/jiskh.v11i2.775
<![CDATA[Introduction; Cholesterol is a metabolite of fat that contains sterol compounds and is primarily concentrated in cell membranes in the body and the circulatory system. Purpose; To determine the total cholesterol level in homemakers (IRT) based on the age range category of 25-80 years and the relationship between increasing total cholesterol levels with the age of IRT. Method; The research method is descriptive-analytical with a cross-sectional approach, with a total sample of 30 and the sampling technique is purposive sampling. Data on the relationship between total cholesterol levels and age were statistically analyzed by bivariate analysis with the Pearson correlation index on SPSS software. Results; Total of 30 IR respondents, the average cholesterol level was 198.07 mg/dL, and the average age was 47.87 years. 50% of respondents have good cholesterol levels, 26.67% in the border category, and 23.33% in the danger category, with the highest cholesterol levels being 297 mg/dL and the lowest being 130 mg/dL. Conclusion; Total cholesterol levels in IRT in Tanjung are dominated by women over 45 years, with cholesterol levels ranging from 130 to 300 mg/dL, and there is a strong relationship between increased cholesterol levels in the body and rising age]]>
<![CDATA[Sosialisasi Dan Monitoring Kebersihan Tandon Air Di Desa Mandiraja Wetan Sebagai Upaya Peningkatkan Status Kesehatan ]]>
<![CDATA[Fajar Husen]]>;
<![CDATA[Nuniek Ina Ratnaningtyas]]>;
<![CDATA[Nilasari Indah Yuniati]]>;
<![CDATA[Nur Aini Hidayah Khasanah]]>;
<![CDATA[Ulfa Fadila Rudatiningtyas]]>
<![CDATA[ Jurnal Pengabdian Masyarakat Indonesia Vol 2 No 4 (2022): JPMI - Agustus 2022 ]]>
Publisher : <![CDATA[CV Infinite Corporation ]]>
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DOI: 10.52436/1.jpmi.723
<![CDATA[Kebersihan air untuk kepentingan masyarakat di Desa Mandiraja Wetan, khususnya RT 07 RW 02 menjadi hal yang sangat penting, mengingat penggunaan air yang banyak digunakan untuk konsumsi, usaha, dan MCK. Pentingnya kebersihan air tandon air perlu di evaluasi dan investigasi serta dimonitoring agar kualitas air di tandon air dapat terjaga. Metode pengabdian masyarakat ini adalah dengan wawancara langsung dan pemberian kuisioner berupa pertanyaan. Hasil wawancara kemudian disajikan dalam bentuk data persentase dan histogram (deskriptif analitis) yang dibuat dengan microsoft excel. Tujuan dari pengabdian masyarakat ini adalah untuk mengetahui profil dan status kebersihan tandon air serta agar warga dapat lebih memperhatikan kebersihan tandon air tersebut guna meningkatkan status Kesehatan. Hasil pengabdian masyarakat ini menunjukan bahwa ada sekitar 60 kepala keluarga (KK), dimana 75% masyarakatnya memiliki tandon air berupa toren, dan hanya 16,7% tidak memiliki tandon air, dan sisanya 8,3% tandon air dalam bentuk cor. Selain itu 40% masyarakat selalu membersihkan tandon air pada rentang 6-12 bulan, dan hanya 20% yang membersihkan pada rentang 1-3 bulan dan warga membersihkan tandon air dengan frekuensi 1-2 kali yaitu 52% dan hanya 5% 4 dengan 4 kali.]]>
<![CDATA[Profil Mikokimia dan Aktivitas Antidiabetes Jamur Coprinus comatus pada Tikus Model Hiperglikemia dengan Induksi Streptozotocin ]]>
<![CDATA[Nuniek Ina Ratnaningtyas]]>;
<![CDATA[Fajar Husen]]>
<![CDATA[ Jurnal Mikologi Indonesia Vol 6, No 1 (2022): Juni 2022 ]]>
Publisher : <![CDATA[Perhimpunan Mikologi Indonesia ]]>
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DOI: 10.46638/jmi.v6i1.204
<![CDATA[Jamur paha ayam (Coprinus comatus (O.F.Müll.) Pers.) merupakan kelompok jamur pangan yang dapat dimakan serta berpotensi sebagai obat. Senyawa mikokimia jamur ini sangat beragam seperti alkaloid, saponin, steroid, terpenoid dan flavonoid yang memiliki aktivitas sebagai antidiabetes. Pengembangan obat herbal alternatif untuk diabetes mellitus (DM) dengan menggunakan C. comatus hasil kultivasi lokal di CV. Asa Agro Corporation Cianjur, perlu dimaksimalkan mengingat manfaat yang besar dari jamur ini. Tujuan riset untuk mengetahui jenis senyawa mikokimia jamur C. comatus dan aktivitasnya dalam menurunkan tingkat glukosa dalam darah pada tikus model hiperglikemik. Metode penelitian ini adalah true experiment¸ post-test only dengan kelompok kontrol. Sebanyak 24 individu tikus yang dibagi menjadi enam kelompok yang tersusun menjadi kontrol sehat (KS: tikus tanpa perlakuan), kontrol negatif (KN: diinduksi streotozotocin (STZ) 45 mg), kontrol positif (KP: diberi metformin 45 mg), kelompok perlakuan dengan pemberian ekstrak (terdiri dari P1: diberi 250 mg, P2: diberi 500 mg dan P3: diberi 750 mg). Pemberian ekstrak dan metformin dilakukan sekali setiap pagi hari. Nilai glukosa dalam darah diukur pada hari ke-empat setelah dilakukan induksi STZ, dan pada hari ke 15 setelah pemberian ekstrak dan metformin. Identifikasi senyawa mikokimia ekstrak etanol dilakukan dengan metode kualitatif dan kuantitatif menggunakan kromatografi gas spektrometeri massa (GC-MS). Hasil menunjukkan ekstrak etanol tubuh buah C. comatus mengandung senyawa flavonoid, alkaloid, terpenoid dan saponin. Ekstrak dari C. comatus juga mampu menurunkan kadar glukosa darah puasa secara signifikan (p < 0.05) dengan persentase penurunan tertinggi yaitu 28% dengan pemberian ekstrak dosis 250 mg. Dosis 500 mg mampu menurunkan tingkat glukosa dalam darah saat puasa dengan 26,71%, serta pada dosis 750 mg menurunkan 27,90%. Hasil ini dapat menjadi informasi ilmiah untuk dapat dilakukan pengembangan lebih lanjut dari jamur C. comatus sebagai kandidat obat herbal alternatif untuk DM.]]>
<![CDATA[An Overview of Stress-Tolerant Promoting Endophytic Fungal Isolates from Hiyung Chilli Grown in South Kalimantan: Endophytic Fungi of Hiyung Chilli Peppers ]]>
<![CDATA[Witiyasti Imaningsih]]>;
<![CDATA[Nuraeni Ekowati]]>;
<![CDATA[Salamiah]]>;
<![CDATA[Nuniek Ina Ratnaningtyas]]>;
<![CDATA[Loekas Soesanto]]>
<![CDATA[ Journal of Tropical Life Science Vol. 13 No. 1 (2023) ]]>
Publisher : <![CDATA[Journal of Tropical Life Science ]]>
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DOI: 10.11594/jtls.13.01.18
<![CDATA[Endophytic fungi are a unique class of microorganisms that lives in plant tissues. These fungi could spread from roots to shoots and interact with nearly all plants in a symbiotic, mutualistic, or commensal manner. Endophytic fungi are believed to exert several roles related to plant growth, including the ability to tolerate stress, especially in Hiyung chilli plants. This plant is widely cultivated in Indonesia and is a well-known host for endophytic fungi. The Hiyung chilli is a popular variety grown in the South Kalimantan Province, which receives national recognition and is geographically certified by the Indonesian government. This wetland-cultivated chilli plant has a uniquely high capsaicin content and long fruit shelf life compared to other varieties. Although endophytic fungal isolates are prevalently found on chilli plants, their potential to influence the growth of their host remains to be proven beyond a doubt. This review emphasizes the sparse reports on the potential of these antagonistic fungi in protecting chilli plants against other pathogens by producing auxin/Indole-3-Acetic Acid (IAA) and capsaicin. The study hypothesised that the antagonistic abilities of these endophytic fungi against other fungal pathogens are related to their IAA- and capsaicin production that synergistically increase the chili plants' threshold against biotic and abiotic stress.]]>
<![CDATA[Korelasi Dan Profil Asam Urat, Kadar Kolestrol, Dan Usia Pada Pekerja ]]>
<![CDATA[Fajar Husen]]>;
<![CDATA[Nuniek Ina Ratnaningtyas]]>;
<![CDATA[Nur Aini Hidayah Khasanah]]>;
<![CDATA[Ulfa Fadilla Rudatiningtyas]]>
<![CDATA[ Jurnal Bina Cipta Husada Vol 19 No 1 (2023): Jurnal Bina Cipta Husada ]]>
Publisher : <![CDATA[STIKes Bina Cipta Husada ]]>
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<![CDATA[Penyakit degeneratif yang menyerang orang lanjut usia memberikan kontribusi yang besar terhadap laju mortalitas, seperti hiperkolestrol, serangan jantung dan stroke. Kolestrol dalam tubuh merupakan hasil dari proses metabolisme lemak. Selain faktor tingginya kolestrol, asam urat yang tinggi juga memberikan dampak negatif yang besar. Asam urat yang berlebih dapat membentuk kristal yang berbentuk tajam, terutama terkonsentrasi di sendi dan jaringan di sekitarnya sehingga memberikan efek peradangan/ inflmasi, rasa sakit dan nyeri. Pentingnya evaluasi dan pengukuran level asam urat dan kolestrol pada pekerja yang memiliki aktivitas dan mobilitas yang tinggi perlu dilakukan, sehingga dapat menjadi peringatan awal agar pekerja dapat memperhatikan lebih kesehatannya. Tujuan dari penelitian ini adalah untuk mengetahui korelasi dan profil antara level asam urat dan kolestrol dengan usia pekerja di Desa Mandiraja Wetan RT 07/RW 02. Metode penelitian ini adalah deskriptif analitik, total sampel adalah 25 dengan 15 perempuan dan 10 laki-laki yang bekerja setiap hari. Pendekatan riset adalah cross-sectional dengan sampling method yang digunakan purposive sampling. Data dianalisis dengan SPPS melalui uji bivariate dan person-correlation versi 26.1. Hasil penelitian menunjukkan bahwa 24% responden didominasi oleh peternak ikan, dan terendah adalah peternak ayam dengan 8%. Rata-rata kadar kolestrol tertinggi pada responden laki-laki adalah 274 mg/dL, terendah 173 mg/dL sementara pada responden perempuan tertinggi 271 mg/dL dan terendah 156 mg/dL. Kadar asam urat tertinggi dan terendah responden laki-laki adalah7.5 mg/dL dan 5 mg/dL, asam urat perempuan 8.7 mg/dL dan 4.5 mg/dL. Persentase kadar kolestrol dengan status baik yaitu 53.33% pada responden perempuan, dan persentase asam urat tertinggi dengan kategori tinggi pada responden perempuan dengan 53.33%. hasil uji korelasi menunjukkan terdapat korelasi yang signifikan antara kadar asam urat, kolestrol dan usia dengan p < 0.05.]]>
