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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Publikasi Pendidikan AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Media Kedokteran Hewan E-Journal Widya Kesehatan dan Lingkungan Quagga Bioeksperimen: Jurnal Penelitian Biologi Jurnal Florea Pro-Life Biodidaktika : Jurnal Biologi dan Pembelajarannya Jurnal Biodjati Jurnal Penelitian Pendidikan IPA (JPPIPA) Faktor Exacta Diklabio: Jurnal Pendidikan dan Pembelajaran Biologi JMM (Jurnal Masyarakat Mandiri) Bio Educatio : The Journal of Science and Biology Education Martabe : Jurnal Pengabdian Kepada Masyarakat BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains QARDHUL HASAN: MEDIA PENGABDIAN KEPADA MASYARAKAT Sainmatika: Jurnal Ilmiah Matematika dan Ilmu Pengetahuan Alam JURNAL CEMERLANG: Pengabdian pada Masyarakat Bioedusiana: Jurnal Pendidikan Biologi EDUKATIF : JURNAL ILMU PENDIDIKAN BIOSFER : Jurnal Biologi dan Pendidikan Biologi JURNAL PENDIDIKAN MIPA Jurnal Biogenerasi Community Development Journal: Jurnal Pengabdian Masyarakat Didaktika: Jurnal Kependidikan Journal of Biology Learning Bioscientist : Jurnal Ilmiah Biologi EduBiologia: Biological Science and Education Journal Journal for Lesson and Learning Studies BIOMA : Jurnal Biologi dan Pembelajarannya Edukasiana: Jurnal Inovasi Pendidikan Jurnal Riset Rumpun Matematika dan Ilmu Pengetahuan Alam (JURRIMIPA) Jurnal Pendidikan, Sains dan Teknologi Jurnal Nusantara Berbakti Jurnal Media Akademik (JMA) Jurnal Medika: Medika BIO-SAINS | Jurnal Ilmiah Biologi Pentagon: Jurnal Matematika dan Ilmu Pengetahuan Alam Algoritma: Jurnal Matematika, Ilmu Pengetahuan Alam, Kebumian dan Angkasa Jurnal Pendidikan Kimia, Fisika dan Biologi Jurnal Cakrawala Pendidikan dan Biologi (jucapenbi) Jurnal Biosilampari: Jurnal Biologi Jurnal Dinamika Pendidikan
Rina Hidayati Pratiwi
Universitas Indraprasta PGRI
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Bioactivity of Antibacterial Compounds Produced by Endophytic Actinomycetes from Neesia altissima Pratiwi, Rina Hidayati; Hanafi, Muhammad; Artanti, Nina; Pratiwi, Rosa Dewi
Journal of Tropical Life Science Vol 8, No 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (835.918 KB) | DOI: 10.11594/jtls.08.01.07

Abstract

Identification of alternate bioactive compounds isolated from microbial endophytes from plants may lead to better solution against antibiotic resistance. Main objective of this research is to determine the bioactivity of antibacterial compounds produced by endophytic actinomycetes, which was isolated from stem bark of Neesia altissima Bl. at Halimun Salak Mount. Detection of bioactive compounds was assayed using Thin Layer Chromatography (TLC) by employing bioautography. Bioactivity was assayed using crude extract against five microorganisms using agar diffusion methods. Tetracycline was used as a positive control. The result of antibacterial activity from endophytic actinomycetes isolate revealed that the bioactive compounds were effective in inhibiting the growth of bacteria Bacillus cereus (1.25 mm), Salmonella typhimurium (5.75 mm), and Shigella flexneri (4.37 mm). Bioactivity of ethyl acetate extract from bioactive compounds of endophytic actinomycetes has an antibacterial activity against bacteria Bacillus cereus (13.00 mm) and Staphylococcus aureus (15.90 mm), at a concentration of 250,000 ppm. Each of TLC fraction was able to inhibit growth of B. cereus at a minimum concentration of 10,000 ppm. In conclusion, the bioactive compounds produced by endophytic actinomycetes can have inhibitory effect towards B. cereus. The category of that compounds are narrow spectrum. The bioactive compound isolated from endophytic actinomycetes may have other implications such as anticancer, antifungal, and antioxidant.
Bioactivity of Antibacterial Compounds Produced by Endophytic Actinomycetes from Neesia altissima Rina Hidayati Pratiwi; Muhammad Hanafi; Nina Artanti; Rosa Dewi Pratiwi
Journal of Tropical Life Science Vol. 8 No. 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.01.07

Abstract

Identification of alternate bioactive compounds isolated from microbial endophytes from plants may lead to better solution against antibiotic resistance. Main objective of this research is to determine the bioactivity of antibacterial compounds produced by endophytic actinomycetes, which was isolated from stem bark of Neesia altissima Bl. at Halimun Salak Mount. Detection of bioactive compounds was assayed using Thin Layer Chromatography (TLC) by employing bioautography. Bioactivity was assayed using crude extract against five microorganisms using agar diffusion methods. Tetracycline was used as a positive control. The result of antibacterial activity from endophytic actinomycetes isolate revealed that the bioactive compounds were effective in inhibiting the growth of bacteria Bacillus cereus (1.25 mm), Salmonella typhimurium (5.75 mm), and Shigella flexneri (4.37 mm). Bioactivity of ethyl acetate extract from bioactive compounds of endophytic actinomycetes has an antibacterial activity against bacteria Bacillus cereus (13.00 mm) and Staphylococcus aureus (15.90 mm), at a concentration of 250,000 ppm. Each of TLC fraction was able to inhibit growth of B. cereus at a minimum concentration of 10,000 ppm. In conclusion, the bioactive compounds produced by endophytic actinomycetes can have inhibitory effect towards B. cereus. The category of that compounds are narrow spectrum. The bioactive compound isolated from endophytic actinomycetes may have other implications such as anticancer, antifungal, and antioxidant.
Co-Authors ., Kartikasari A.A. Ketut Agung Cahyawan W Abdul Wahid, Sunar Acep Musliman Agung Windrianto Ahmad Subeki Akhiroh, Muhibatur Rohmatul Alamsyah, Mashudi Almaida Garudea Putri Andri Suryana Anggraeni, Ayu Ani Hoerunisa Annisa, Tiara Nur Apriliani, Trisna Astiningrum, Rania Dwita A’liyah, Umi Habibahtul Bada, Sitti Sastriana Budiarso, Iwan Bunga Bunga Ceni Liswati CLEOPATRA, MARIA Darwis, Puji Deanti Raka Siwi Deni Nasir Ahmad Dewi, Shinta Dinni Epri Anggraeni Dodo Murtado Dulhamin Arif Dwi Aprillia Setia Asih Efri Gresinta Endang Suistyaningsih Endang Sulistyaniningsih Endang Sulistyaniningsih Endang Sulistyaniningsih Erfin Nurfalah Fachny Wardiani Fajar Sodik Fajri, Riesto Atantio Ferry Fauzi Ferry Fauzi, Ferry Fery Rahmawan Fika Arie Susanti Firdaus, Rizka Fitriany Fitri Damayanti Gilang Medal Pangrestu Giry Marhento Hasbullah Hasbullah Hasbullah Hasbullah Hilda Mardiyana Ira Miranti Jafar Akmaludin Jepri, Jumadi Jihan Fadhilah Jumadi Jepri Jumadi Jepri Kurniasari Ambar Kurniasari Sulistyorini Laksita Nirmala Putri Lengsi Manurung Mamik Suendarti Mariasih, Mariasih Mega Cantika Meriyanti, Meriyanti Merry Lapasau Minerva Anindyta Mohammad Ananda Reza Kurniawan Mohzana Muhamad Sofiandi Muhammad Hanafi Muhammad Husin Muhammad Ikhsan Muhammad Ridwan Nadirah, Nadirah Naza, Kasyifatun Naza Neli Permatasari Netty Demak Sitanggang Nina Artanti Nina Artanti Noer, Shafa Noviarani, Annisa Novita Trianah Nur Imah Nurina Fitarini Nurul Khoiriyah Nurzeini Herdiansyah Paulina Rendra Endang Pina Aprilliani Pramasti, Aliza Pratiwi, Rosa Dewi Pratiwi, Rosa Dewi Priambodo, Caka Gatot Puspita Zahra Putri, Desti Nurdiana Eka Putri, Laksita Nirmala Rahmi Nur Salamah Rakhman, Fathor Riesto Atantio Fajri Rizka Fauziah Rosa Dewi Pratiwi Sahat T. Simorangkir Saptiawati Saptiawati Sari Nolia Sari, Valentina Permata Septiani, Carlista Mega Septianzah, Kevin Shafa Noer Shandi Noris Sihite, Julius Siti Khoirunnisa Siwi, Deanti Raka Sriyono Sriyono Sulistyaniningsih, Endang Syahid Syifa Pazriyati Tanjung, Emilda Tantry Agnhitya Sari Theressia Evy Nadeak Virgana Wijayani, Anisa Yeni Nurdiana Yosy Ezha Ramadhanty Yudha Ningsih Yulianah Yulianah Yuliani, Irma Yulistiana Yulistiana Yulistiana Yulistiana Yulistiana Yusi