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Journal : Tropical Genetics

Phylogenetic analysis in silico of orchid species Dendrobium based on rbcl marker Febriansyah, Muhammad; Mahfut
Tropical Genetics Vol. 4 No. 2 (2024): Genetics
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v4i2.68

Abstract

Dendrobium is one of the genera of the third largest Orchidaceae family which reaches about 1184 species in the world. In Indonesia, this genus of orchids is a wealth of genetic resources that are abundant in the Eastern region, such as Papua and Maluku. These genetic resources have not been optimally utilized as elders in crossbreeding to produce offspring that have characteristics according to what consumers want. Due to the limitations of these morphological characteristics, another character is needed that can support the systematics of orchids, namely molecular characters. Plant kinship analysis can use molecular characters in the form of DNA strands that can overcome the weaknesses of morphological data that are known to have limitations and tend to be influenced by the environment. This study aims to analyze the kinship of several types of Dendrobium based on the rbcL gene and add to the basic data source of germplasm from the genus Dendrobium. The samples used were nucleotide sequences of 11 nucleotide sequences consisting of 10 types of Dendrobium (D. anosmum, D. jinghuanum, D. catenatum, D. nobile, D. jenkinsii, D. pahangense, D. nobile, D. pulchellum, D. fimbriatum, and D. pulchellum) which is used as an in group and 1 species of the genus Phalaenopsis namely Phalaenopsis equestris which is used as an outgroup. The results of Dendrobium kinship analysis with an in silico based rbcL gene molecular approach show that species in the same genus are not necessarily closely related. The species that is a member of the out group is Phalaenopsis equestris,. Phalaenopsis equestris forms its own tree branches, while the other 10 species are on the same phylogeny tree branches.
Co-Authors Afandi, Aril Afif, Arriza Maulana Akhmad Siddiq, Akhmad Albetian Pangalila, Yonas Alfandi, Aril Alifiansyah, Fadlan Nur Alzril, Muhammad Ardian Ardian Ari Darmastuti ARI KURNIAWAN Arizka Warganegara Arsela Eko Listiono Ashari, Minanti Mayda Budiarman, Andi Azizah Maulidia Deny Setiawan Dewi Indah Noviana Pratiwi, Dewi Indah Noviana Dinata, Riadi Marta Dwicahya, Fazryan effendi, Harlan Effendy, Vina Ardelia Eko Agus Srihanto, Eko Agus Elly Lestari Rustiati Elvinawati Ervianti, Natalia Fahrezi, Aditya Febriyanto, Satrio Fikri Fahresi, Daffa Filda Angellia Firdauzi, Muhammad Fithriyyah, Hulwah Fitri Rahmafitria, Fitri Hafiz Saputra, Saiful Haqqul Yaqin Heidiani Ikasari, Ines Hertanto Hertanto, Hertanto Heru Susetyo Hidayat, Deski Hisyam, Muhamad Ali Hustia, Anggreany I Nyoman Candra Idhafi, Nasrul Ilahidayah, Wahyumi Imtitsal, Aulia Indah Ayu Lestari, Indah Ayu Ingrid Nurtanio Ja'far, Suhermanto Jaya, Rahmat Junaidi , Ahmad Krisbintoro, R Sigit Kusuma, Andriyani Wijaya Laidinar, Shaubi Lely Shofa Imama Lena Rosida Lestari, Septi Wahyu Maharani, Annisa Lidya Mahfut Mahilaveda, Nadila Mahmud Arjiansyah, Abiem Mochammad Imron Awalludin MUHAMMAD ALI Muliana, M Mursalin, M Muslima Muslima, Muslima Nabila, Anggun T. Nur Amalina Nurhamidah Oktaviana, Meta Pazira, Zira Perani Rosyani Permatasari, Nindy Pratama, Alpin Pratiwi, Dian Neli Priyambodo Priyambodo Priyambodo Raden Mohamad Herdian Bhakti Ramadhan, Viki Ramdani, Farhan Robi'ah, Robi'ah Roselina Panghiyangani Sa'uddah, Lousanja D. Sandra, Shifa Saputri, Irna Saswiyanti, Enny Sidik Permana, Dwi Sidik, Mahfud Son Haji, Yopi Suhada Suhada, Suhada Sundari, Ella Susanto, Alvin Wiwiet Susilawati, Susilawati Syabifi, Fiqla Syarif Hidayat Teti Desyani Thesalonika, Natasya Tri Haryanto, Agus Utami Dewi, Dyah Utami, Juliyatin Putri Wahyu Hidayat, Ahmad Wahyu Tyas Pramono WINARNO Winarno Winarno Yamin, Muhammad Ikrar Yani, Ani Andri Yuliana Andriyani Yusuf, Mukarramah Zukhruf, Elevina