Garuda - Garba Rujukan Digital

Induksi Pertumbuhan Tunas Tanaman Manggis (Garcinia mangostana L.) Secara In Vitro Asri, Elita; Harahap, Fauziyah; Hasairin, Ashar
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13681

This study aims to determine the effect of plant growth regulators and seed cutting patterns on the induction of mangosteen shoot growth. The research was conducted at the YAHDI Tissue Culture Laboratory in Medan, North Sumatra, Indonesia, in 20245. The method used in this study was a Completely Randomized Design (CRD) with two factors: BAP concentration and seed cutting pattern. The data collected were analyzed using statistical analysis of variance (ANOVA) with Duncan's Multiple Range Test (DMRT) at a 5% significance level using SPSS 26. The results of the study show that the interaction between BAP concentration and seed cutting pattern significantly affected the time of shoot emergence, the number of leaves, and the shoot height. The best shoot emergence time was obtained with a BAP treatment of 4 mg/L using whole seed explants, which occurred at 1 MST (Month After Sowing). A BAP concentration of 2 mg/L with whole seed explants produced the highest number of leaves, with 8 leaves. The highest number of shoots was obtained from explants planted in a medium with 6 mg/L BAP, which resulted in 5.67 shoots. Whole seeds planted in a medium with 0 mg/L BAP produced the tallest plants, measuring 8.9 cm. The combination of BAP concentration and seed cutting pattern did not significantly affect the number of roots.

Development of Tissue Culture Textbook Based on Project Based-Learning to Improve Science Process Skills of Biology Department Students Universitas Negeri Medan Harahap, Salamah; Harahap, Fauziyah; Hasanah, Dwi; Sembiring, Gres Anjelia; Panggabean, Syakilah Kurnia Fahmi; Nadhilla, Annisa; Suriani, Cicik; Edi, Syahmi; Daulae, Abdul Hakim
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13786

The research on the development of this textbook was carried out based on the results of the analysis of student needs, namely that there are no teaching materials that can improve students' science process skills in the Tissue Culture course at the Department of Biology, State University of Medan. This textbook is compiled based on the learning syntax of Project-Based Learning (PjBL). This study aims to develop a Project-Based Learning-based tissue culture textbook to improve students' science process skills. This research method uses the Research and Development (R&D) with Thiagarajan's 4D (Define, Design, Develop, and Disseminate). The population of this study was students of the Biology Department of Universitas Negeri Medan with a sample of 47 people who were given a PjBL-based tissue culture textbook. The results showed that (1) the textbooks have been tested for feasibility by material experts (87,73) (2) instructional design experts (92) with a very feasible category, (3) the response of tissue culture lecturers (92) and (4) students based on individual tests (95.1), small group tests (93,75), and limited group tests (90.02) with a very good category and (5) the results of the calculation of N-Gain for science process skills were obtained at 0.66 which is in the medium category.

Pengaruh IAA dan Urutan Daun Terhadap Pertumbuhan Tunas Tanaman Anggrek Cattleya sp. Lubis, Anita Agustina; Harahap, Fauziyah
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12996

Orchids are ornamental plants that have high aesthetic value and beauty. Many human activities use this plant, including weddings, Christmas, New Year and birthdays. Orchid propagation can be done conventionally, but it requires a large area of land and takes a long time to grow. However, it can also be multiplied using tissue culture. Plant tissue culture techniques have better prospects than conventional propagation because tissue culture can produce large numbers of new plants without being influenced by time or season. This research aims to determine the effect of IAA and leaf order on shoot growth of Cattleya sp orchid plants. This type of research is experimental research with a Factorial Completely Randomized Design (CRD) with 12 treatments and 3 replications. There are 2 factors in this study, namely factor I PGR IAA (0, 0.5 mg/l, 1 mg/l, and 1.5 mg/l) and factor II Leaf order (1st leaf, 2nd leaf, and 3rd leaf). This research is a quantitative descriptive study with the parameters of the time of shoot emergence and using 2-way Statistics with Analysis of Variance (ANOVA) with the parameters of number of shoots, number of leaves (strands), and plantlet height. From the research results, it was found that the administration of IAA (Indole Acetic Acid) and the order of the leaves did not have a real effect on the number of shoots, number of leaves (strands) and plantlet height. However, the best treatment is the I0.5D2 treatment (IAA 0.5 mg/l and 2nd leaf).

Induksi Perakaran Anggrek (Dendrobium sp.) Secara In Vitro dengan Penambahan Indole Butyric Acid (IBA) dan Bubur Pisang Raja (Musa paradisiaca L) Harahap, Fauziyah; Sudibyo, Mufti; Sari, Elvira Nanda; Suriani, Cicik; Edi, Syahmi; Panggabean, Nurul Huda; Daulay, Abdul Hakim
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.15402

This study aims to obtain a good concentration of IBA and plantain and is suitable for root induction in Dendrobium sp. The benefit of this study is to provide information about the successful root induction of orchid plantlets Dendrobium sp. In vitro culture and creating formulations using IBA hormones and organic plantain ingredients in in vitro culture media optimally. This research has been carried out at the YAHDI Plant Tissue Culture Laboratory. Rooting orchids Dendrobium sp. In vitro using MS basic media which was added with IBA (Indole 3 Butyric Acid) 0 ppm, 2 ppm, 4 ppm, and 6 ppm, and added with plantain pulp with levels of 0 g/l, 100 g/l. and 200 g/l. This study was a factorial experiment with 2 factors compiled based on a 4 x 3 Complete Random Design (RAL) with 12 treatment combinations. The data obtained was analyzed for variance analysis (ANOVA). If there are results that have a real effect, it is followed by the DMRT (Duncan Multiple Range Test) test at the level of 5%. (1) The results of the study showed that the treatment of the variables of the number of roots, plant height, and root length was influenced by IBA. (2) The treatment of the variables of the number of buds, the number of leaves, the number of roots, the height of the plant, and the length of the roots is influenced by plantains. (3) The treatment of the variables on the number of roots, root length, and leaf width were influenced by the interaction of IBA and banana pulp.

Optimalisasi Sterilisasi Eksplan Daun Kopi Arabika Sidikalang (Coffea arabica L.) Untuk Penanaman In Vitro Lubis, Theresia; Harahap, Fauziyah; Damanik, Jesika Febriani; Batubara, Wardah Arijah; Adelia, Ichlasul; Apriliani, Dwi; Sohada, Ummi
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 3 (2025): September
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i3.17664

This study aimed to determine the effect and identify the optimal sterilization pattern for suppressing contaminant growth in the in vitro culture of Sidikalang Arabica coffee (Coffea arabica L.) leaf explants. The experiment was conducted using a completely randomized design (CRD) with five treatment levels and 15 replications. Data were analyzed using the Kruskal Wallis test, and if significant differences were found, the Mann Whitney test was applied at a 5% significance level. The results showed that sterilization patterns significantly affected the time to contamination onset, the percentage of contaminated explants, the percentage of browning explants, and the percentage of explants forming callus. However, no significant effect was observed on the time of callus initiation. Sterilization pattern 5 was identified as the most optimal in suppressing contamination, with the lowest contamination percentage (6,6%), browning (6,6%), and the highest callus formation (93,3%). This pattern involved washing with detergent; soaking in Agrept (30 minutes) and Benlate (30 minutes); followed by sterilization in a laminar airflow cabinet (LAFC) using Tween 20 (20 minutes), graded Bayclin at 10%, 20%, and 30% (for 20, 15, and 5 minutes, respectively), 70% alcohol (20 seconds), and ascorbic acid (20 minutes).

Pengaruh IAA dan Urutan Daun Terhadap Pertumbuhan Tunas Tanaman Anggrek Cattleya sp. Lubis, Anita Agustina; Harahap, Fauziyah
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12996

Orchids are ornamental plants that have high aesthetic value and beauty. Many human activities use this plant, including weddings, Christmas, New Year and birthdays. Orchid propagation can be done conventionally, but it requires a large area of land and takes a long time to grow. However, it can also be multiplied using tissue culture. Plant tissue culture techniques have better prospects than conventional propagation because tissue culture can produce large numbers of new plants without being influenced by time or season. This research aims to determine the effect of IAA and leaf order on shoot growth of Cattleya sp orchid plants. This type of research is experimental research with a Factorial Completely Randomized Design (CRD) with 12 treatments and 3 replications. There are 2 factors in this study, namely factor I PGR IAA (0, 0.5 mg/l, 1 mg/l, and 1.5 mg/l) and factor II Leaf order (1st leaf, 2nd leaf, and 3rd leaf). This research is a quantitative descriptive study with the parameters of the time of shoot emergence and using 2-way Statistics with Analysis of Variance (ANOVA) with the parameters of number of shoots, number of leaves (strands), and plantlet height. From the research results, it was found that the administration of IAA (Indole Acetic Acid) and the order of the leaves did not have a real effect on the number of shoots, number of leaves (strands) and plantlet height. However, the best treatment is the I0.5D2 treatment (IAA 0.5 mg/l and 2nd leaf).

Induksi Pertumbuhan Tunas Tanaman Manggis (Garcinia mangostana L.) Secara In Vitro Asri, Elita; Harahap, Fauziyah; Hasairin, Ashar
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13681

This study aims to determine the effect of plant growth regulators and seed cutting patterns on the induction of mangosteen shoot growth. The research was conducted at the YAHDI Tissue Culture Laboratory in Medan, North Sumatra, Indonesia, in 20245. The method used in this study was a Completely Randomized Design (CRD) with two factors: BAP concentration and seed cutting pattern. The data collected were analyzed using statistical analysis of variance (ANOVA) with Duncan's Multiple Range Test (DMRT) at a 5% significance level using SPSS 26. The results of the study show that the interaction between BAP concentration and seed cutting pattern significantly affected the time of shoot emergence, the number of leaves, and the shoot height. The best shoot emergence time was obtained with a BAP treatment of 4 mg/L using whole seed explants, which occurred at 1 MST (Month After Sowing). A BAP concentration of 2 mg/L with whole seed explants produced the highest number of leaves, with 8 leaves. The highest number of shoots was obtained from explants planted in a medium with 6 mg/L BAP, which resulted in 5.67 shoots. Whole seeds planted in a medium with 0 mg/L BAP produced the tallest plants, measuring 8.9 cm. The combination of BAP concentration and seed cutting pattern did not significantly affect the number of roots.

Development of Tissue Culture Textbook Based on Project Based-Learning to Improve Science Process Skills of Biology Department Students Universitas Negeri Medan Harahap, Salamah; Harahap, Fauziyah; Hasanah, Dwi; Sembiring, Gres Anjelia; Panggabean, Syakilah Kurnia Fahmi; Nadhilla, Annisa; Suriani, Cicik; Edi, Syahmi; Daulae, Abdul Hakim
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13786

The research on the development of this textbook was carried out based on the results of the analysis of student needs, namely that there are no teaching materials that can improve students' science process skills in the Tissue Culture course at the Department of Biology, State University of Medan. This textbook is compiled based on the learning syntax of Project-Based Learning (PjBL). This study aims to develop a Project-Based Learning-based tissue culture textbook to improve students' science process skills. This research method uses the Research and Development (R&D) with Thiagarajan's 4D (Define, Design, Develop, and Disseminate). The population of this study was students of the Biology Department of Universitas Negeri Medan with a sample of 47 people who were given a PjBL-based tissue culture textbook. The results showed that (1) the textbooks have been tested for feasibility by material experts (87,73) (2) instructional design experts (92) with a very feasible category, (3) the response of tissue culture lecturers (92) and (4) students based on individual tests (95.1), small group tests (93,75), and limited group tests (90.02) with a very good category and (5) the results of the calculation of N-Gain for science process skills were obtained at 0.66 which is in the medium category.

Optimasi Sterilisasi Eksplan Biji Manggis (Garcinia mangostana L.) Menggunakan Natrium Hipoklorit Secara In vitro Harahap, Fauziyah; Asri, Elita; Hasairin, Ashar; Edi, Syahmi; Suriani, Cicik; Daulae, Abdul Hakim; Panggabean, Nurul Huda
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.15281

This study evaluates the effects of NaOCl concentration and immersion time on contamination levels and viability of in vitro mangosteen seed explants, aiming to identify the optimal sterilization protocol. Conducted at YAHDI Tissue Culture Laboratory, Medan, Indonesia, a Randomized Complete Block Design tested three treatment combinations: (1) 15% and 10% NaOCl for 5 minutes, (2) 20% and 15% NaOCl for 15 minutes, and (3) 30% and 20% NaOCl for 25 minutes. After immersion, explants were rinsed three times with sterile distilled water. Observations spanned 12 MST, with data analyzed using ANOVA and Games-Howell post hoc tests; contamination sources were qualitatively described. Results showed the first combination had the lowest effectiveness, the second was better but suboptimal, while the third significantly delayed contamination and yielded the highest explant survival rate of 86.1%. The third treatment also prolonged the contamination-free period compared to others. Fungal and bacterial contaminants were predominant, highlighting the need for effective disinfectants against both. These findings indicate that the third combination is a promising sterilization protocol for mangosteen seed explants, especially for hard endocarp tissues prone to contamination.

Title

Found 9 Documents
Search
Journal : Bioscientist : Jurnal Ilmiah Biologi

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 9 Documents Search Journal : Bioscientist : Jurnal Ilmiah Biologi

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 9 Documents
Search
Journal : Bioscientist : Jurnal Ilmiah Biologi