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Kadar Glukosa Darah dan Infestasi Ektoparasit Flagellata pada Ikan Mas Koki (Carrasius auratus) yang diberi Suspensi Whole Cell Lernaea dengan Dosis dan Waktu Pemeliharaan yang Berbeda Netty Sreani; Laksmi Sulmartiwi; Gunanti mahasri
Journal of Aquaculture Science Vol 8 No 1 (2023): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v8i1.241

Abstract

The high consumer interest in ornamental fish such as goldfish causes aquaculture to try to meet the highest production so that large scale aquaculture activities appear accompanied by problems due to imbalances between the host, the environment and diseases such as pathogens. The purpose of this study was to determine the effect of giving Whole Cell Lernaea as an immunostimulant development material as an effort to prevent pathogen attacks in aquaculture. This study used a completely randomized design (CRD) with a factorial pattern, namely factor A was different doses of Whole cell Lernaea and factor B was different length of maintenance. The results showed that the highest blood glucose levels of goldfish occurred in the 0 ppm treatment on day 0 with a range between 53.60-87.60 mg/dL and the lowest in the 5 ppm treatment on day 21 with a range between 44.20-86.60 mg/dL. Ichthyobodo spp. ectoparasite infestation. occurred on days 0 and 14 with a dose of 0 ppm which is about 2-3 ind/head different from giving a dose of 5 ppm during maintenance for 21 days there were no cases of goldfish that were attacked by ectoparasites. There was an interaction between dose and duration of maintenance on blood glucose levels of goldfish given Whole cell lernaea suspension, while there was no interaction with flagella ectoparasite infestation. The best treatment to reduce blood glucose levels to normal is the administration of Whole Cell Lernaea suspension at a dose of 5 ppm and a maintenance time of 21 days.
Intensity and Infestation Degree of Ectoparasite on White Leg Shrimp (Litopenaeus vannamei) Cultivated in Intensive Ponds Yusma Latifatul Ummah; Gunanti mahasri; Laksmi Sulmartiwi
Journal of Aquaculture Science Vol 9 No 1 (2024): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/joas.v9i1.51011

Abstract

White leg shrimp (Litopenaeus vannamei) is a widely cultivated commodity in Indonesia. The high demand of white leg shrimp requires increased production. High stocking density in intensive cultivation and excessive amounts of feed are obstacles in white leg shrimp productiom. It will cause the shrimp to become stressed and susceptible to pathogen attack , one of them is ectoparasite. This research was conducted from August to September 2023 with the aim of determining the intensity and infestation degree of ectoparasite on white leg shrimp that cultivated in intensive ponds. The samples of white leg shrimp in this study were 50 individuals by purposive random sampling method. Observations were carried out by natif method in the Cultivation and Anatomy Laboratory, Faculty of Fisheries and Marine, Airlangga University. The results showed that white leg shrimp cultivated in intensive ponds were infested by Zoothamnium sp. with intensity 184 individuals/head, Vorticella sp. with intensity 268 individuals/head and Epistylis sp. with intensity 235 individuals/head. Each ectoparasite has a very severe infestation degree
Nilai Indeks Fagositosis (IF) dan Total Vibrio Count (TVC) Udang Vaname (Litopenaeus vannamei) Pada Tambak Intensif Putri Garnet Endo Mahata; Gunanti mahasri; Laksmi Sulmartiwi
Journal of Aquaculture Science Vol 9 No 1 (2024): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/joas.v9i1.54415

Abstract

Whiteleg shrimp (Litopenaeus vannamei) is one of the main fishery commodities since it has a high economic value. Vaname shrimp cultivated is done intensively where the stocking density and the amount of feed required are high. High stocking densities can trigger stress, increase possibility of physical contact among shrimps, and the spread of disease in shrimp becomes faster. The most common shrimp disease is Vibriosis where whiteleg shrimp are infected by Vibrio sp.. Whiteleg shrimp has an innate immunity system thus hemolymph has an important role in shrimp body defense. In this study, the research method used was purposive random sampling and was conducted from August to September 2023 by 50 shrimps sample. Assay of phagocytosis index (IF) which is a cellular response of the vaname shrimp body defense system was conducted at the Center for Brackish Water Aquaculture (BBPBAP) Jepara by applying a bacterial suspension of Staphylococcus sp. which has been attenuated and bacterial staining. Calculation of Total Vibrio Count (TVC) was conducted at the Laboratory of Bacteriology and Mycology, Faculty of Veterinary Medicine, Universitas Airlangga, by using TCBS (Thiosulfate Citrate Bile Salt Sucrose Agar) agar media from Vibrio sp. bacteria cultured from the hepatopancreas of shrimp samples. The results showed that vaname shrimp cultured in intensive ponds were infected with Vibrio sp. with a TVC value of 3.3x105 cfu/ml and an IF value of 0.87.
KOMBINASI NANOPARTIKEL KITOSAN DAN MODIFIED TAPIOCA-WHEY PROTEIN DALAM PEMBUATAN EDIBLE COATING TERHADAP AKTIVITAS ANTIBAKTERI DAN ORGANOLEPTIK OTAK-OTAK TENGGIRI Farras Rana Hafizhah; A. Shofy Mubarak; Gunanti Mahasri
Journal of Aquaculture Science Vol 9 No 2 (2024): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/joas.v9i2.62039

Abstract

Product otak-otak ikan tenggiri is a promising fishery diversification product but is prone to spoilage. Edible coatings can help slow down spoilage. The purpose of this study was to determine the effect of different edible coating formulations on the characteristics of edible coating and to determine the protective potential of edible coatings against bacterial growth and organoleptic product otak-otak ikan tenggiri during storage. This research is an experimental study that is divided into two stages, the first stage is the characteristics of edible coatings using a completely randomized design (CRD) with testing including antimicrobial activity, water absorption, and viscosity with 5 treatments and 4 replicates, and the second stage is coating applications on the length of storage of product otak-otak ikan tenggiri testing including TPC and organoleptic using two-factor CRD 3×6 treatments and 3 replicates. Results indicated a significant impact (P < 0.05) of coating formulations on these characteristics. The best formulation, modified tapioca-WPI with CSNP (P4), showed antibacterial activity with a 9.16 mm inhibition zone, 3.46% water absorption, and 15.33 cP viscosity. This formulation maintained microbial stability and organoleptic quality for up to 3 days at room temperature and 12 days under cold temperature.
KOMBINASI NANOPARTIKEL KITOSAN DAN TURUNAN SELULOSA DALAM PEMBUATAN EDIBLE COATING TERHADAP AKTIVITAS BAKTERI DAN ORGANOLEPTIK PEMPEK IKAN TENGGIRI Fadiah Shabrina; Gunanti Mahasri; A. Shofy Mubarak
Journal of Aquaculture Science Vol 9 No 2 (2024): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/joas.v9i2.62040

Abstract

Pempek is a very popular traditional Indonesian food because it has a delicious taste. However, the shelf life of pempek is only about 16 hours at room temperature. This study aims to develop and evaluate edible coatings based on cellulose and chitosan nanoparticles (CSNPs) to extend the shelf life of pempek from Tenggiri fish (Scomberomorus commerson). This study is experimental research divided into two stages. The first stage involves characterizing the edible coating using a Completely Randomized Design (CRD), with tests including antimicrobial activity, water absorption, and viscosity, conducted with 5 treatments and 4 repetitions. The second stage involves applying the coating to pempek and evaluating its shelf life, focusing on TPC and organoleptic qualities using a two-factor CRD with 3×6 treatments and 3 repetitions. The results showed a significant effect (P<0.05) of various formulations on the characteristics of edible coatings. The results showed that the variation of cellulose derivatives and CSNP in the preparation of edible coating significantly affected the coating characteristics (P<0.05). The combined formulation of CMC and CSNP (P5) was selected as the pempek coating, with antibacterial activity of 9.31 mm, water absorption of 1.23%, and solution viscosity of 33.50 cPa.s. In the second stage, the treatment of pempek coating with CMC and CSNP (C2) effectively maintained the quality of pempek until day 3 at room temperature and day 12 at refrigerator temperature.
Stress Response and Pathology of Hybrid Groupers (Epinephelus lanceolatus × Epinephelus fuscoguttatus) Infested Trichodina sp. Maintained in Hatchery from Bali, Indonesia Lia Oktavia Ika Putri; Gunanti Mahasri; Ahmad Shofy Mubarak; Lilis Cahaya Septiana
Journal of Aquaculture Science Vol 10 No 1 (2025): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/joas.v10i1.67885

Abstract

The demand for grouper fish continues to increase, with the Ministry of Fisheries' annual production target of 9,000 tons. However, intensive cultivation systems pose risks due to poor water quality, making grouper fish susceptible to disease and health problems. This study investigated the impact of Trichodina sp. infection on the blood glucose levels of hybrid grouper fish. The study was conducted in May 2024 by observing 30 fish and measuring blood glucose. The results showed that 20 fish showed glucose levels above the normal range (> 57.6 mg/dL), ranging from 61 to 114 mg/dL. In contrast, nine fish had glucose levels in the normal range (28.8 - 57.5 mg/dL), and one fish had low glucose levels of 26.7 mg/dL. High glucose levels in most fish indicate stress or metabolic disorders, possibly related to Trichodina sp. infestation. This parasitic infection can compromise the fish's immune system, making the fish more susceptible to disease. The results emphasize the need for effective parasite management and optimal water quality in intensive culture systems. To maintain grouper health and productivity, it is important to monitor water quality, control ectoparasites, and conduct further research on the long-term effects of these infections on blood glucose and overall health.
Identification of Vibrio Species and Total Vibrio Count in Kidney Tissue of Humpback Grouper (Cromileptes altivelis) Reared at Different Stocking Densities in Pond-Based Net Cages Nafila Junia Azadia; Gunanti Mahasri; Abdul Manan
Journal of Aquaculture Science Vol 11 No 1 (2026): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/joas.v11i1.78913

Abstract

The humpback grouper (Cromileptes altivelis) is a high-demand grouper species, both domestically and internationally. However, the utilisation of this cultivation system still faces obstacles, especially in the field of disease. This research was conducted to determine the total Vibrio count (TVC) and identify Vibrio species in the kidney tissue of humpback grouper reared with different stocking densities in pond-based net cages. The TVC examination method was carried out using serial dilution and grown on selective TCBS media. Bacterial identification was carried out by observing colony colour, Gram staining, and biochemical tests (catalase, SIM, TSIA, OF, and MRVP test). The results show that the highest TVC value was obtained from high-density treatment (1.9 × 104 CFU/g), meanwhile, the lowest TVC value was obtained from normal-density treatment (0.1 × 101 CFU/g). The results of bacterial identification showed that 3 of the 5 isolates obtained during the study (V1, V2, V4) were similar to V. fluvialis, V. alginolyticus, and V. parahaemolyticus based on biochemical tests. This study concluded that Vibrio bacteria similar to V. fluvialis, V. alginolyticus, and V. parahaemolyticus were found in the kidneys of grouper fish cultured at different stocking densities in net cages, and that the TVC values remained within the normal range.
Cyprinus carpio hematological changes after dietary Coffea canephora leaf extract for Aeromonas hydrophila infection Shandy Sulistyoningrum; Gunanti Mahasri; Mohammad Faizal Ulkhaq; Hapsari Kenconojati
Depik Vol 12, No 2 (2023): AUGUST 2023
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.2.28682

Abstract

Coffea canephora or robusta coffee was known as a medicinal plant that admitted various biological properties. However, its application as a medicinal plant in fish disease treatment has not been reported. This study aimed to investigate the effect of dietary robusta coffee leaf (RCL) extract on the survival rate and hematology of Cyprinus carpio infected by Aeromonas hydrophila. Two hundred fish were randomly divided into five treatment groups consisting of uninfected fish (UF), infected fish (IF), and a diet supplemented with different concentrations of RCL extract (30%, 40%, and 50%). The negative control group was injected by NaCl 0.9% and other treatment groups were injected by A. hydrophila. Feeding treatment was carried out for 7 days after clinical symptoms appeared. The results showed that RCL extract increases the survival rate and hematological parameters (number of erythrocytes, hemoglobin, and lymphocytes) significantly (P0.05). The highest survival rate (100%) was recorded in groups that accepted 50% of RCL extract. This current study indicates that RCL extract could be considered a potent medicinal plant for treating fish disease caused by A. hydrophila at the optimum dose of 50%.Keywords:AeromonasCoffea canephoraExtractHematologyCyprinus carpio
A Study of The Perceptions of Trawl Fishermen Towards The Rules of The Trawl Ban on The North Coast of East Java M Iqbal Satria; Adriana Monica Sahidu; Gunanti Mahasri; Fuad
Grouper Vol. 15 No. 2 (2024): Grouper : Jurnal Ilmiah Perikanan
Publisher : Universitas Islam Lamongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30736/grouper.v15i2.233

Abstract

The issuance of the cantrang prohibition regulation, namely Permen Kp No. 2 of 2015, is based on the decline in Fish Resources (SDI) which threatens sustainability, so for the sake of sustainability it is necessary to impose a ban on the use of trawls and seine nets including cantrang. This study will analyze how the perception of the seine net fishing community towards the rules of the cantrang ban. This study used survey methods, questionnaires and interviews and was conducted at PPP Mayangan Probolinggo and PPP Bulu Tuban. The number of samples in this study were 20 respondents with purposive sampling. Data analysis using Likert scale, validity test and reliability test. The results showed that if the government emphasizes the rules of the cantrang ban, cantrang fishermen will really lose income, causing increased unemployment, especially for crew members. On the other hand, the Ministerial Regulation will have a positive impact for the future in the form of sustainability of marine fish resources.
Improvement of Immune Defense and Survival of Grouper Fish (Ephinepelus sp.) Using the Immuno-Probiosirkulasi System (SI-PBR) Method in Semi-Intensive Ponds in Brondong, Lamongan Gunanti Mahasri; Woro Hastuti Satyantini; Nina Nurmalia Dewi; Lia Oktavia Ika Putri; Lilis Cahaya Septiana; Elangga Sony Widiharsono; Salman Aldo Alfaresi; Faisol Mas’ud; Fuquh Rahmat Shaleh; M. Khairul Anam; Ika Purnamasari
Grouper Vol. 15 No. 1 (2024): Grouper : Jurnal Ilmiah Perikanan
Publisher : Universitas Islam Lamongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30736/grouper.v15i1.243

Abstract

Grouper (Epinephelus sp.) is a marine fishery commodity that is widely cultivated in marine cages and ponds. The export market demand for grouper fish continues to increase every year by 30.75% every year, so there is a need for improvement, through various technological applications. The main obstacle in cultivating grouper fish in ponds is the decline in water quality and disease attacks, which can cause up to 100% crop failure, just 3 – 5 days after infection. One effort that can be implemented is the cultivation method with the Immuno-Probiocirculation System (SIPBR). Brondong sub-district is one of the areas in Lamongan which has large fisheries potential, especially for breakwater water pools, which are the top among other districts. There have been many cases of grouper deaths in ponds to date, which are caused by disease attacks and water quality. The aim of this community service activity is to apply the Immuno-Probioculation System fish aquaculture technology in traditional plus pattern ponds (SI-PBR), increasing grouper fish production in Lamongan Regency, East Java. Observational method which includes socialization / counseling, planning and guidance for implementing SI-PBR technology in one period (three months). These results show positive indications. There is an increase in farmer knowledge, the SI-PBR method can reduce ectoparasite infestation and increase grouper yields from 272.43 kg / ha to 87.66 kg / ha, meaning an increase of 334%. The conclusion from this activity is that the SI-PBR model can increase fish production and can be applied in wider areas in the Lamongan region.
Co-Authors A. Shofy Mubarak Abdul Manan Abdul Manan Abdul Manan Abyan Farras Adamu Ayubu Mwendolwa Adamu Ayubu Mwendolwa Adelia Inas Shafira Adiacahya, Eren Aditya Gita Rohmatullah Afifah Elok Firdaus Agniny, Siswi Fililmi Agung Pamuji Rahayu Agus Nazarudin Yahya Ahmad Shofy Mubarak Ahmad Shofy Mubarak Akhmad Taufiq Mukti Alanosi Noor Muhammad Alfan Prianggara Alfin Tauhid Alim Isnansetyo Almira Fardani Lahay Alvira Febrianti Pratiwi Anggun Khoirun Nikmah Anggun Nurani Citrowati Anisa, Hosia Anord Charles Nkuba Apri Supii Ardilas Heryamin Arika Juniarsih Arya Witantama Berliana A Bernathdo Mahendra Robbi Putra Bidayatul Afifah Boedi S. Rahardja Boedi Setya Rahardja Browijoyo Santanamurti Cintia Larasati DARMAWAN SETIA BUDI Daruti Dinda Nindarwi Daud Dharmansyah Desak Ketut Sekar Cempaka Putri Dheani Nur Ahadya Syifa Chaerunissa Dieswinta Hardika Aris dika dika Dita Wisudyawati Dwikinta, Made Anindya Eka Saputra Elangga Sony Widiharsono Elangga Sony Widiharsono Elisabeth Benita Indarmastuti Endah Sih Prihatini Endah Sih Prihatini Endah Sih Prihatini Endang Dewi Masithah Era Insivitawati Ewang Mahendra Putra Fadiah Shabrina Fahdi Putra Utama Faisol Mas&#039;ud Faisol Mas’ud Faisol Mas’ud Faisol Mas’ud Faisol Mas’ud Farid Nur Salim Farizka Vinka Trinendyah Farras Rana Hafizhah Ferry Dwi Firmansyah Liananda Fida Fransisca Firly Waliani Rahma Fuad Fuquh Rahmat Shaleh Fuquh Rahmat Shaleh Fuquh Rahmat Shaleh Fuquh Rahmat Shaleh Gian Suryanatha Hartawan Hari Suprapto Heru Suryanto Ika Purnamasari Ika Putri, Lia Oktavia Ikmalia A Ikmalia Amali Indah Hidayati Imani Iqbal Ghazali Irvansyah Irvansyah Isroni, Wahyu Kadek Racmawati Karunia, Fitria Kenconojati, Hapsari Kismiyati , Koesnoto Koesnoto Kusnoto Kusnoto Lailatul Lutfiyah, Lailatul Laksmi Sulmartiwi Laksmi Surmartiwi Larasati, Anastasya Dewi Lestari Wilujeng Lia Oktavia Ika Putri Lia Oktavia Ika Putri Lia Oktavia Ika Putri Lia Oktavia Ika Putri Lilis Cahaya Septiana Lilis Cahaya Septiana Lilis Cahaya Septiana Linnya Prima Agustin Lucia Tri Suwanti, Lucia Tri Luthfiana Aprilianita Sari Lyla Wulandari M Ervany Eshmat M Iqbal Satria M. Khairul; Anam M. Muhtar Mandele Maulana, Muhammad Hilmy Mayangsari, Cholivia Melinda Kusuma Ningrum Miftakhul Munir Moch Saad Mochammad Dwi Hardhianto Mohamad Yusuf Mohammad Faizal Ulkhaq Mufasirin Muhamad Amin Muhamad Amin Muhammad Amin Muhammad Arief Muhammad Herman Muhammad Musa N. Juni Triastuti Nafila Junia Azadia Nafis Putra Laksana Cholil Nanik Siti Aminah Nanuk Qomariyah Nedi Nedi Netty Sreani Nico Rahman Caesar Niluh Suwasanti Nina Nurmalia Dewi Norma Isnawati Nugroho, Sefilia Adhi Nunuk Dyah Retno Lastuti Nur Fais Nurlita Abdulgani Nurul Kumalasari Ochtavia, Sherly Permata Jelita, Chelsea Prayogo Prayogo Pristita Widyastuti Puguh Yugo Wijanarko Putra, Bernathdo Mahendra Robbi Putri Garnet Endo Mahata Putri, Desak Ketut Sekar Cempaka Rachma Woro Anggarani Racmawati, Kadek Rahayu Kusdarwati Rahayu Kusdarwati Ridwansyah Ririn Agustiya Riris Ulfiana Riza Aryani Romziah Sidik Rozi Rozi Rr. Juni Triastuti Sahidu, Adriana Monica Salman Aldo Alfaresi Salman Aldo Alfaresi Samara, Syifania Hanifah Santanumurti, Muhammad Browijoyo Sapto Andriyono Saputra, Alfian Rahmadhani Satria Hani Sari, Putri Desi Wulan Saroso, Heri Setiawan Koesdarto Setyantini, Woro Hastuti Shandy Sulistyoningrum Sherly Ochtavia Shohifah, Isnatul Umu Siti Hamidah Sri Mulyati Sri Mulyati Sri Subekti Sri Subekti Sri Subekti Sudarno Sudarno Sudarno, Sudarno Suwasanti, Niluh Suzanita Utama Titom Gusmana Putra Perdana Ulia Fajriah Veryl Hasan W. Angan Indrawan Widodo, Langgeng Wijanarko, Puguh Yugo Wiwin Sumiati Wiwin Sumiati Woro Hastuti Satyantini Woro Hastuti Satyantini Wurlina, W Yanuhar, Uun Yudi Cahyoko Yunifar Amad Yusma Latifatul Ummah