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Sri Purwaningsih
Departemen Teknologi Hasil Perairan, Fakultas Perikanan Dan Ilmu Kelautan, Institut Pertanian Bogor, Bogor 16680. Jln. Lingkar Kampus IPB Dramaga-Bogor Tlp./Fax. (0251) 8622915/(0251) 8622916, Sripurwa65@yahoo.com; Sripurwa65@gmail.com
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Journal : Berkala Perikanan Terubuk

 1 
AKTIVITAS ANTIOKSIDAN PADA FORMULA TABLET TERIPANG KELING (Holothuria atra) Ahmad Fauzan Lubis; Sri Purwaningsih; Kustiariyah Tarman
Berkala Perikanan Terubuk Vol 44, No 2 (2016): Juli 2016
Publisher : Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (394.002 KB) | DOI: 10.31258/terubuk.44.2.51 - 69

Abstract

This study about activity of antioxidant aimed to determine the chemical characterization of sandfish, sandfish meat and sandfish powder, determine the best formulation in manufacturing Holothuria atra tablet based on DEPKES RI standard and to determine the antioxidant activity and the active compounds that act as antioxidants in the extract of Holothuria atra tablets. The method used in the manufacture of tablets is the direct compression method using 6 formulations to treat the kind of powder substance that is the ingredient of sandfish are intact and substances from the meat of sandfish. Tablet chosen based on the standards of the Ministry of Health that includes the physical characteristics of the test tablet and shelf-life stability. Tablet rivet sandfishtested the antioxidant activity by the method of 2,2-diphenyl-1-picrylhydrazyl (DPPH). Tests to determine the active compounds that act as antioxidants with thin layer chromatography (TLC) and bioautography antioxidants and also to determine the functional groups using analysis of Fourier Transform Infrared (FTIR). The yield of sandfish powder is considerably low. Yield of sandfish powder are 10.26% and sandfish meat powder are 9,82%. The protein content of sandfish powder are 60,89% lower compared to sandfish meat powders of 63,57%. Formula elected from the sandfish tablet test based on physical characteristics and stability test of the shelf life of the tablet is the formula A2 with the composition of the powder formulation Holothuria atra (25%), Ac-Di-Sol (2%), L-HPC (2%), Aspartame (0.5%), menthol (0.2%), talk (1%), magnesium stearate (1%) and lactose: mannitol (67.8%) and has antioxidant activity with IC50 value of 97.22 ppm. TLC test and Bio-autography showed that the class of antioxidant compounds are steroid, and suspected compounds at FTIR test, that act as antioxidants compound has similarities to the sterol class.
Co-Authors -, Maison AA Sudharmawan, AA Abdul Khairul Rizki Purba Mustofa Indwiani Astuti Adrianus Inu Natalisanto Agoes M Jacoeb Agoes Mardiono Jacoeb Ahmad Fahrudin Ahmad Fauzan Lubis Akbar Kurniawan, Mohammad Alam, M. Sastra Alamsyah Harahap Allen A. CH. Manongko Alrizal, Alrizal anak agung putu ayu puspita negara Anggri Alfira Yunita Assa Anna Carolina Erungan Annisa Rahma Fatmala Apema, Elcye Apit Setiani Arianda, Yoga Dwi Arifa Mustika Arpan, Susan Gracia Asrial Azizahwati Azizahwati Azizahwati Azizahwati Azrifitria ., Azrifitria Bagus Sediadi Bandol Utomo Bambang P. Priosoeryanto Benny Manulang Denny Susanto Dewi Gayatri Dewi Ulfa Trisdiani Dinny Devi Triana Dwi Puspita Sari D’Arqom, Annette Effionora Anwar Eka Ayuningrat Eka Deskawati Ekowati Handharyan Ekowati Handharyani ella - salamah Ella Salamah Ella Salamah Ella Salamah Ella Salamah Ella Salamah Ella Salamah Ellis Permatasari Ema Hastarini Enie Novieastari Ermawati Ermawati Erna Setiany Estelle Lilian Mua Evy Damayanthi Fahriza Sri Afni Farida Nugrahani Fatimah, Nurmawati Febri Berthalita Pujaningsih Fedora, Katherine Fitriany Podunge G A Arwati, I Gian P Apriyana Giawan Nur Fitria Gina Mustika Haerul Pathoni, Haerul Harahap, Nur Kholija Hari Setiyawati Hasti Kusuma Hastuti, Isnaini Budi Hebat Shidow Falah I Gede Restawan I'tishom, Reny Indah Ria Lestari Indiastuti, Danti Nur Indriani, Rin Insani Putri, Fhadira Islamia, Saribatul Izzatul Fithriyah Janice Sepang Jefri Marzal Joko Santoso Jules Nurhatmi Juliana Neng Ribka Jumain Jumain Jumain Khaerunnisa, Siti Komarayanti, Sawitri Kustiariyah Tarman Lasut, Maria Lestari, Neneng Lusianti, Erita Luthfianti, Yasinta M. Nur Adnin M. Rahmad Maftuchah Maftuchah Mala Nurilmala Mamanua, Steven Maretty Twentyna Dolorosa Mar’atun Rohmah Susanti Masikah Maylan Dewi Meilin Anggreyni MERLINDA KEMALA DEWI Mousa, Almahdi Muhammad Sahal Mukti Widayati Mutmainnah. HS Nada, Hairul Nehru Nehru Nikmawatisusanti Yusuf Nova Natalia Beba Novallyan, Devie Nugroho, Cahyo Adhi Nurhikma Nurhikma Nurina Hasanatuludhhiyah Nurjanah Nurjanah Olga Purnama Bakti Pebralia, Jesi Pratiwi, Intan Pratiwi, Riri Puji Rahayu Purwani Istiana Purwanti Ningsih Puspita Sari, Dwi Putri Julia Mizulni Rahman, Dewi Rahmayani Rahmatia Garwan Rahmatia Garwan Ramacos Fardela Ramadoni, Ramadoni Rasmi, Dian Pratiwi Reki A. Febriani Reryd Arindany Wiryawan, Reryd Arindany Reza Dewantoro Ria Ambarwati Riantoni, Cicyn Rieke Pernamasari Rika Kurnia Rimbawan , Rinto . Rista Bintara Rista Bintara, Rista Riviani Riviani Riyan Triono Ropawandi, Doni Rosmawati Peranginangin Ruddy Suwandi Sabarulin Sabarulin Sabintoe, Merlin Safira, Safira safrina dyah hardiningtyas Sartikasari, Dwi Sereli Pia Setiyadi, Agus Silmi Mariya Sinulingga, Fahri Siswanto, Anilla Adelia Siti Choiriah Siti Madanijah Siti Sarpingah Sitinjak, Hardiantinus Soeprijanto Sofia Alvionita Sri Murni Sugeng Heri Suseno Suharmadi Suharmadi Syeni Budi Anita TATI NURHAYATI Taufik Hidayat Tika A. Budiarti Tiza Yunisca Sari Triyani Budyastuti Utamayasa, I Ketut Alit Villany Golwa, Gian Watania, Dolly Wijayanti, Dyah Ayu Wini - Trilaksani Wisnu Jaka Dewa Wiwit Suprihatiningsih Yennita, Y Yosi Hasana Yuani Setiawati Yudhi Herliansyah Yusnaidar, Yusnaidar Yustina Yustina Yuversa, Edi Ziarah, Hikmah