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Fajar Restuhadi
Program Studi Agribisnis Universitas Riau
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Journal : emerging science journal

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Cellulose Microfiber Encapsulated Probiotic: Viability, Acid and Bile Tolerance during Storage at Different Temperature Usman Pato; Dewi Fortuna Ayu; Emma Riftyan; Fajar Restuhadi; Wasisso Tunggul Pawenang; Royyan Firdaus; Annisa Rahma; Irwandi Jaswir
Emerging Science Journal Vol 6, No 1 (2022): February
Publisher : Ital Publication

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28991/ESJ-2022-06-01-08

Abstract

This work aimed to analyze the physicochemical properties of cellulose from OPT used in the fabrication of CMF and evaluate the efficacy of the hydrogel CMF as an encapsulant for L. fermentum InaCC B1295 stored at room temperature and in the refrigerator. The Kjeldahl method was used to evaluate the protein content; the gravimetric method was used to determine OPT's ash, moisture, and fiber contents; the Soxhlet method was used to determine the fat content carbohydrates were computed using the difference method. The levels of holocellulose, lignin, and cellulose were also determined. Viability, acid and bile resistance of strain B1295 were evaluated at various temperatures for 35 days. The most abundant component of OPT fiber was cellulose, followed by hemicellulose and lignin. XRD examination revealed that OPT cellulose has a crystal index of 83.40%. FTIR analysis was used to detect the stretching vibrations of the –OH group on cellulose at 3419.03 cm-1. CMF hydrogel from OPT sustained L. fermentum InaCC B1295 survival for up to 28 days at room and refrigerated temperatures. At acidic conditions and in the presence of bile, the viability of L. fermentum InaCC B1295 was excellent, with a drop in cell population of less than 0.2 log CFU/g over 35 days at room and refrigerated temperatures. CMF obtained from OPT can be used as an encapsulant to maintain viability, acid resistance and bile of probiotics. There is still a need for research into the usage of CMF from OPT in combination with other encapsulants to extend the storage life of L. fermentum InaCC B1295. Doi: 10.28991/ESJ-2022-06-01-08 Full Text: PDF
Co-Authors ', AKHYAR ALI ', Akhyarali ', Eliza ', FADRO ', Mukmin ', Rahmayuni ' ', RASWEN EFENDI ', RIGUAN EPENDI ', RIKO PAMORI Abid, Hussein Rasool Adi Ruslan Agung Setiawan Framadya Agus Ika Putra Ahmad Fadli Ahmad Ibrahim Ahmad Ibrahim Roni Surya Hasibuan Ahmad Rifai Ahmad Rifai Ahmad Rifai Ahmad Rifai Akhmad Ibrahim R. S. Hasibuan Akhyar Ali AKHYAR ALI ' Akhyarali ' Alexander Hutagaol Alfi Mawaddah Almira Nugroho Amun Amri Andri Gunawan Purba Andriyani Fadmi Angelia Angelia Angga Pramana Angraini, Elisya Anjelia, Yuni ANNISA RAHMA Annisa Rahma Ari Sandhyavitri Arifudin Arifudin Arifudin arifudin Ariyanda Putra Arum Rovarti Ningsih Arvin Arvin Azhar, Muhammad Rizwan Beltaser Tuahta Benhard F Situmorang BUNGA RIA YULIANA SIAHAAN Carles Pernando Cepriadi Chairul ' Ciary Jannah Mangkay Conni Simarmata, Conni Dahliyusmanto, Dahliyusmanto Debby Anggina Dede putra pranata Delvi Andika Derita Lamtiar Pasaribu Desi Heltina Desy Tri Artika Devinda Desi Indra Murti Dewi Fortuna Ayu Dian Martha Darman Dian Martha Darman Didi Muwardi Dini Aji Pringgondani Diyah Suriani Djaimi Bakce Dodi Saputra Dodi Saputra Doni Putra Erlinda Duafrizal Joycorleon Manik Edo Saputra Eka Ramadhansyah Eliza Eliza ' Eliza Eliza Elka Roza Elsa Novitasari Elsyintia Dwi Putri Emma Riftyan Eri Sayamar Erick Gunawan Bahar Erick Gunawan Bahar Et, Elfa Azani Evy Maharani Evy Rossi Fadel Al Barra FADLA B. SYARIF FADRO ' Faizah ' Hamzah Faizah Hamzah Faizah Hamzah Fajar Yuliandri Fanny Septya Febrianto, Wegi Eko Febrina Ayuningtyas Febrizal Ujang Fitri Santi Fitri, Mozhalita Desta Gervilon Darvis Gustrinazul Hamidi, Muhammad Rasyid Hamzah, Farida Hanum Hanifaturrahmi Andrina Hanifaturrahmi Hanifaturrahmi Hartini, Fitri HARUN SIANTURI Hendri Winardo Ikhsan, Muhammad Ikhsan Ilham Apandi Imam Hidayat Imam S. Dalimunthe, Imam S. Imanuel ' Sianipar Indra Budiman Indradewa, Rhian indriana, fitrisya Ingrid Suryanti Surono IRWANDI JASWIR Irwandi Jaswir Ismail Haridsyah Iva Ancewita Saragih Jayawarsa, A.A. Ketut Jonrio Canway Juliati Samosir Juliati Samosir Jumatri Yusri Jum’atri Yusri Kausar ' Kausar Kausar Khairunnisa Amanda Kurnia, Deby Lela Natalia Lena Marjelita Lukmanasari, Putri M. Bayu Purwanto M. Rasyid Hamidi Manik, Marjohan ' Matius Teddy Ginting Megawati Megawati Meisela, Elsy ' Meki Herlon MITA SAPUTRI Mitha Aprilia Monica Valentina Therescova Simbolon, Monica Valentina Therescova Muhammad Akbar Hasibuan Muhammad Luthfi Mukmin ' Mustaqim Mustaqim Nadia Novianti Tamba Nadia Novianti Tamba Novia Dewi Novia Dewi Novian Novian, Novian Noviar Harun Novrianto ' Nuke Fatmasari Nuke Fatmasari Nur Hasnah AR Nuratika, Nuratika Nurfitasari, Mimi Nurwijayanti OKTANIYA OKTANIYA OKTANIYA, OKTANIYA Panca Setia Utama Pangestu, Dio Ajie PARLUHUTAN PARLUHUTAN PARLUHUTAN, PARLUHUTAN Pasaribu, Jekson Pradita Arnandasari Prawiranegara, Barata Aditya Putri Siska Junianti Putri Wahyuni Putri, Hilma Anisa Quan Paulus Giovan Rahmawati Ulfah Rahmayuni Rahmayuni Rano RASWEN EFENDI Raswen Efendi RASWEN EFENDI ' Ratih Ardia Sari Rezita Azizah Rici ' Mainaki Ricki Mustapa Ridanil Hanif RIGUAN EPENDI ' Rika Afrianto RIKO PAMORI ' Rizki Ananda Rizza ' Novita Rosma, Iswadi Hasyim Rosnita Rosnita Rosnita Rosnita Rosnita Rosnita Rosnita ROYYAN FIRDAUS Royyan Firdaus Roza Yulida Ryan E Syahrial Sabam Mael Beri M Sadzvirani, Sastiana Sakti Hutabarat Santi Andriani Putri, Santi Andriani SAPUTRI, MITA Sari, Manja Sebayang, Aulia Shafira Shorea Khaswarina SIANTURI, HARUN Simatupang, Dewi Sispa Pebrian Soewignjo Agus Nugroho Sri Haryati Sugiono Sugiono Sujantara, Defri Aire Sukri Rizki Ilahi Susy Edwina Syafrini Rizki Rahmah SYAIFUL BAHRI Syaiful Hadi Taufik Setiawan Taufiq Maulana Taufiq Maulana Tengku Dahril, Tengku Teresia, Yossi Tika Fadilah Tika Fadilah Tri Azrul Disyamto Turnip, Titus T Ulfa Rizki Pradini Ulfah, Fatia Usman Pato Vina Octavia Vonny Setaries Johan Vonny Setiaries Johan Wahyu Sartika WASISSO TUNGGUL PAWENANG Wasisso Tunggul Pawenang Winda Kusuma Dewi Wiranata, Leo Candra Yelmira Zalfiatri Yeni Kusumawaty Yossie Kharisma Dewi Yulia Andriani, Yulia YULIANA SIAHAAN, BUNGA RIA Yuliandri, Fajar Yunita Ariani Zebua Yunita Ariani Zebua Yusmarini Yusmini Yusri , Jum'atri Yusri, Jum'atri Zulhamid Ridho Zulhardi, Rizky Zulkifli