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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Jurnal Primatologi Indonesia Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Hemera Zoa Media Veteriner Forum Pasca Sarjana Jurnal Sain Veteriner Buletin Veteriner Udayana Jurnal Veteriner Biologi Edukasi : Jurnal Ilmiah Pendidikan Biologi Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Ilmu Ternak Veteriner Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) ARSHI Veterinary Letters JURNAL BIOLOGI PAPUA Jurnal Kedokteran Hewan Jurnal Veteriner dan Biomedis

Srihadi Agungpriyono

Bogor Agricultural University

Author-ID : 386019

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary Other

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Spermatogenesis and semen quality of male muntjak (Muntiacus muntjak muntjak) during antler growth periods Sri Wahyuni; Srihadi Agungpriyono; Muhammad Agil; Idawati Nasution; . Hamny; Tuty L. Yusuf
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Muntjak (Muntiacus muntjak muntjak) belongs to Cervidae family which distributed in Java Island and Southern part of Sumatera. This cervid has been protected by Indonesian Government since 1999. In order to support breeding program of the species and to avoid them from extinction, itsreproductive biology such as spermatogenesis and the correlation to semen quality is important to be investigated. Therefore, the objective of this study was to examine spermatogenesis and semen quality of two adult male muntjaks during antler growth periods that consist of hard antler (H), casting (C), andvelvet antler (V). Testicular tissues and semen (ejaculates) were obtained by core needle biopsy and electroejaculation methods respectively. Testicular tissues were processed histologically and stained with periodic acid Schiff (PAS) to observe spermatogenesis whereas semen was evaluated to obtaine itsquality. The results showed that spermatogenic activities were detected in all antler periods which marked by PAS positive staining (magenta colour) of round and elongated spermatid acrosomes. In H period, spermatogenic activity was higher than those C and V periods. According to semen evaluation,motile spermatozoa were found with different concentration in all antler periods. The highest sperm concentration (x 106 spermatozoa/ml) in both of muntjaks was found in H period (506.25 ± 61.87), and slightly decreased in C (288.75 ± 37.12), and V periods (362.60 ± 17.68). These finding showed thatspermatogenesis to produce spermatozoa is taken place while muntjaks are in C and V periods with differ activities that provable with the existence of motile spermatozoa from ejaculates in both of male muntjaks. Therefore, muntjaks could provide reproductive function throughout the year of reproductiveaseasonality which is similar to the reeves and formosan muntjaks

Status of ram spermatozoa DNA after freeze-drying process Takdir Saili; wahono Esthi Prasetyaningtyas; Mohamad Agus Setiadi; Srihadi AgungPriyono; Arief Boediono
Jurnal Ilmu Ternak dan Veteriner Vol 11, No 3 (2006): SEPTEMBER 2006
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The process of freeze drying caused detrimental effect on plasma membrane and acrosome of the spermatozoa, even it potentially could alter the chromatin and DNA integrities. On the other hand, DNA integrity is essential for spermatozoa to participate in pronucleus formation during fertilization event. Therefore the evaluation of DNA integrity should be carried out to study the effect of freeze drying process. EDTA, EGTA, and PBS were used as dilution media of spermatozoa prior to freeze drying process to protect the DNA. Toluidine blue staining and comet assay methods were used to evaluate the alteration on chromatin and DNA integrities of spermatozoa, respectively. The results revealed that the highest compacted chromatin after 6 months storage of freeze-dried spermatozoa were observed from EGTA-3 (98%) and EGTA-1 (97%) treatments that had significant differences compared to all PBS treatments (90-92%), but not for fresh spermatozoa (100%). Whereas, the highest compacted DNA integrity of freeze-dried spermatozoa were observed from EGTA-2 (92%) and EGTA-3 (92%) but had no significant differences compared to other treatments including fresh spermatozoa (97%). These results demonstrate that EDTA and EGTA tend to be able to protect chromatin and DNA integrities of ram spermatozoa during freeze-drying and storage compared to PBS. Key Words: Freeze-Drying, Spermatozoa, DNA, Toluidine Blue, Comet Assay

Intracytoplasmic Sperm Injection (Icsi) Sebagai Teknik Reproduksi Bantuan Unggulan Takdir Syahruddin Said; Syahruddin Said; Mohamad Agus Setiadi; Srihadi Agungpriyono; Mozes R. Toelihere; Aries Boediono
Jurnal Sain Veteriner Vol 23, No 1 (2005): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Beberapa teknik fertilisasi mikro telah diterapkan untuk mengatasi masalah infertilitas pria, namun hanya intracytoplasmic sperm injection (ICSI) yang mampu meningkatkan secara nyata keberhasilan fertilisasi, implantasi dan kelahiran. Pada perkembangannya teknik ICSI juga telah diterapkan pada beberapa jenis hewan sebagai suatu model untuk mempelajari kemampuan fertilisasi berbagai jenis spermatozoa yang secara alami atau melalui fertilisasi in vitro tidal( bisa membuahi sel telur. Walaupun keberhasilan teknik ICSI telah dapat mengatasi masalah infertilitas pada pria, teknik ICSI masih mempunyai potensi negatif yang mungkin muncul pada anak hasil ICSI. Namun demikian, hal ini perlu penelitian lebih lanjut untuk membuktikannya.

Anatomi Radiografi Normal pada Kaki Depan Landak Jawa Yuliani Suparmin; Gunanti Gunanti; Deni Noviana; Srihadi Agungpriyono
Buletin Veteriner Udayana Vol. 11 No. 2 Agustus 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

The aim of this project is to develop a detailed accessible set of reference images of the normal forelimb radiographic anatomy in sunda porcupine (Hystrix javanica), including digit,carpus, metacarpal, radius, ulna, and humerus. This project that using 2 healthy sunda porcupines (male and female) was radiograped using digital radiography and standars projection. Four images, illustrating the normal radiographic anatomy of the forelimb were selected and presented along with detailed description. These image are aimed to be of assistence to veterinary surgeons, veterinary students, medic concervation especially in domestic wild animal, and veterinary researchers by enabling understand of the normal radiographic anatomy of the forelimb, and allowing comparison with the abnormal radiographic.

INJEKSI SPERMATOZOA DOMBA HASIL PENGERINGBEKUAN KE DALAM SEL TELUR MENGGUNAKAN TEKNIK INTRACYTOPLASMIC SPERM INJECTION (ICSI) INJECTION OF FREEZE-DRIED RAM SPERMATOZOA INTO OOCYTES USING INTRACYTOPLASMIC SPERM INJECTION, ICSI Takdir Saili; Mohamad Agus Setiadi; Srihadi Agungpriyono; Arief Boediono
Jurnal Veteriner Vol 8 No 1 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Pada penelitian ini dikaji kemampuan spermatozoa domba hasil pengeringbekuan untuk melakukan dekondensasi dan membentuk pronukleus setelah diinjeksikan ke dalam oosit dengan menggunakan teknik intracytoplasmic sperm injection (ICSI). Metode pewarnaan aceto lacmoid digunakan untuk mengevaluasi kejadian dekondensasi dan pembentukan pronukleus pada oosit setelah ICSI. Hasil penelitian menunjukkan bahwa spermatozoa hasil pengeringbekuan dapat melakukan dekondensasi (2%) dan mendukung pembentukan 1PN (34%) tetapi belum mampu mendukung pembentukan 2PN setelah diinjeksikan ke dalam oosit. Sedangkan injeksi dengan menggunakan spermatozoa segar mampu mendukung pembentukan 2PN (30%) dan 1PN (40%). Sebagai kesimpulan dapat dikemukakan bahwa spermatozoa hasil pengeringbekuan mampu melakukan dekondensasi dan mendukung pembentukan 1PN setelah ICSI

Penentuan Siklus Estrus pada Kancil (Tragulus javanicus) Berdasarkan Perubahan Sitologi Vagina Najamudin -; Rusdin -; Sriyanto -; Amrozi -; Srihadi Agungpriyono; Tuty Laswardi Yusuf
Jurnal Veteriner Vol 11 No 2 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The aim of this study was to determine the estrus cycle and the length of estrus in Tragulus javanicuson the basis of its vaginal cytology. Vaginal swabs were collected daily at 7 am for two months. Smears ofthe swab were then prepared on glass slide and they were stained with Giemsa. Vaginal epithelial cells;parabasal, intermediet and superficial cells were counted and their percentages during proestrus, estrusand diestrus were determined. Diestrus was characterized by the absent of superficial cells in the vaginalepithel. Proestrus was characterized by the progressive increase in percentage of intermediet/superficialcells in vaginal epithel, whereas estrus was characterized by the presence of superficial/cornification cellsin most vaginal epithel. On the basis of its vaginal cytology, it was determined that the estrous period offemale Tragulus javanicus was 3 days (48-60 h) and the length was 11 days (10-14 day). The change in thecytology of vaginal epithelial cells appeared to a good marker to determine the estrus cycle phase of theTragulus javanicus

PERUBAHAN VIABILITAS DAN STRUKTUR SUBSELULER SPERMATOZOA DOMBA SETELAH PENGERINGBEKUAN Takdir Saili; I Ketut Mudite Adnyana; Ronny Rachman Noor; Mohamad Agus Setiadi; Srihadi Agungpriyono; Arief Boediono
Jurnal Veteriner Vol 10 No 4 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Several methods i.e. cooling, freezing, and freeze-drying have been widely used to preserve spermatozoa with various degree of success. Freeze-drying appears to provide a method to preserve spermatozoa in a dry state without requiring liquid nitrogen for storing frozen spermatozoa. Freeze-drying procedures can have a detrimental effect on plasma membrane and acrosomal cap of the spermatozoa. In this experiment study, the viability and subcellular changes of freeze-dried ram spermatozoa were evaluated using staining method and scanning electron microscopy. The results revealed that all freeze-dried spermatozoa were dead following evaluation using eosin staining and Hoechst-propidium iodide staining methods. Morover, plasma membrane and acrosomal cap of freeze-dried ram spermatozoa was disrupted observed using scanning electron microscope.

STUDY ON SPERM AGGLUTINATION WITH CHARACTERIZATION OF PLASMACOLLECTED FROM EPIDIDYMIS AND EJACULATE IN RAM Muhammad Haviz; Arief Boediono; M Agus Setiadi; Srihadi Agungpriyono; Mokhamad Fahrudin
Jurnal Veteriner Vol 9 No 4 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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This study was designed to optimalize the use of epididymal or ejaculate sperm and plasma for in vitro fertilization, that sperm agglutination was found at preparation. The rate of sperm agglutination was calculated the head-to-head sperm agglutination that were incubated in Krebs Ringer-(N-(2hydroxyethyl)piperazine-N’-(2-ethenesulfonic acid) or KR-HEPES medium in 38.50C with 5% CO2 at 1, 3, 5 and 7 hours culture in vitro. The rate of head-to-head sperm agglutination were decreased with time treatments. The cauda of sperm agglutination was lower than that caput, corpus epididymal and ejaculate sperm with statistically significant (P<0.01). These result reflected that distribution of anti-agglutinin might be higher in cauda epididymal than that other areas. Number of protein were characterize with SDS-PAGE as follow 11 bands in caput epididymal, 9 bands in corpus epididymal, 2 bands in cauda epididymal and 4 bands in seminal plasma. The higher distribution of protein was found at range 25-40 kDa in epididymal plasma of ram. However, further investigation should be conducted to determine presumptive anti-agglutinin by advance method.

SEBARAN ANTIAGLUTININ SPERMATOZOA DALAM PLASMA YANG DIKOLEKSI DARI EPIDIDIMIS DAN EJAKULAT DOMBA THE DISTRIBUTION OF SPERM ANTIAGGLUTINI IN PLASMA COLLECTED FROM EPIDIDYMIS AND EJACULATE OF RAM Muhamad Haviz; Srihadi Agungpriyono; Arief Boediono; Mokhamad Fahrudin; M Agus Setiadi
Jurnal Veteriner Vol 8 No 1 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Penelitian untuk mengetahui tingkat aglutinasi antarkepala spermatozoa dan sebaran antiaglutinin dalam plasma asal epididimi dan ejakulat telah dilakukan untuk mengoptimalkan pemanfaatannya dalam fertilisasi in vitro. Tingkat aglutinasi dan aktivitas antiaglutinin plasma epididimis dan ejakulat domba dihitung dengan cara menghitung jumlah aglutinasi antarkepala spermatozoa setelah diinkubasikan selama 1, 3, 5, dan 7 jam in vitro dalam media Krebs Ringer- HEPES.

Tingkat Proliferasi Primordial Germ Cells secara In Vitro dalam Medium Kultur dengan Penambahan Leukemia Inhibitory Factor (IN VITRO PROLIFERATION RATE OF MICE PRIMORDIAL GERM CELLS IN THE CULTURE MEDIUM WITH ADDITION OF LEUKEMIA INHIBITORY FACTOR) Wahono Esthi Prasetyaningtyas; Ni Wayan Kurniani Karja; Srihadi Agungpriyono; Mokhamad Fahrudin
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Primordial germ cells (PGCs) are precursors for gamete cells. The totipotency of PGCs allows them to be used as a model for studying cancer and infertility. The study aimed to examine the characteristics of the mice fetus as a source of PGCs, proliferation rate of PGC and the role of LIF in vitro culture of PGCs. This study used genital ridges from 26 fetuses at 13.5 days post-coital (dpc) to isolate the PGCs. Genital ridges dissociation using 0.1% of trypsin and in vitro culture was carried out using the Dulbecco Modified Eagle Medium (DMEM) and incubated at 37 0C and 5% CO2 atmosphere. The fetus was measured and weighed to determine the normal development of the fetus and continued with the identification of the genital ridges after laparotomy performed under a stereomicroscope. Proliferation rate was measured by calculating Population Doubling Time (PDT), and cell viability was observed after in vitro culture for six days. The effect of adding 1000 IU/ml of leukemia inhibitory factor (LIF) was evaluated from two types of treatment in the medium, 1) DMEM added with 15% of fetal calf serum (FCS) (DMEM + S15%) and 2), DMEM was supplemented with 15% of FCS and 1000 IU/ml LIF (DMEM + S15% + LIF1000 IU/ml). Immunohistochemistry staining was carried out on the third-, sixth- and ninth-day of culture to detect the expression of Oct-4 in the PGCs, then cells were counted. The results showed that the fetus as a source of PGCs had normal development. The fetal sizes were 11 mm, and male and female genital ridges could be distinguished by morphology at the age of 13.5 dpc. The proliferation of PGCs was relatively slow with a 1.3 day PDT value with the viability of around 85%. Culture of PGCs with DMEM + S15% treatment showed the percentage of PGCs that expressing Oct-4 decreased from the third day of culture to the ninth day of culture. The culture of PGCs in DMEM + S15% + LIF 1000 IU / ml treatment showed that the percentage of PGCs that expressed Oct 4 increased on the sixth day of culture and decreased on the ninth day of culture. It can be concluded that the addition of LIF can maintain the number of PGCs until the sixth day of culture. LIF is thought to play a role in the regulation of proliferation of PGCs through receptors of LIF (RLIF) and glicoprotein (gp) 130 receptors.

Co-Authors A.S. Satyaningtijas Adi Winarto Adrien Jems Akiles Unitly Afiff , Usamah Alastair A Macdonald Amaq Fadholly Amrozi Anak Agung Istri Sri Wiadnyani Andhika Yudha Prawira Andhika Yudha Prawira Andhika Yudha Prawira Andhika Yudha Prawira Andhika Yudha Prawira Andhika Yudha Prawira Angelina N. Tethool Anisa Rahma Arief Boediono Aries Boediono Aryani Sismin Sastyaningtijas Asri Pudjirahaju Auzi Asfarian Chairun Nisa Chairun Nisa Chairun Nisa' Chairun Nisaâ€™ Chairun Nisa’ Chairun Nisa’ Cutnya’ Shaliran Nazlie (Alm) Damiana Rita Ekastuti Danang C. Cahyadi Danang Dwi Cahyadi Dedi Rahmat Setiadi Deni Noviana Denny W. Lukman Desrayni Hanadhita Desrayni Hanadhita Desrayni Hanadhita Desrayni Hanadhita Desrayni Hanadhita Dondin Sajuthi Dwi Endrawati Dwi Kesuma Sari Dwinna Aliza Eragilang Muhammad Hastapatria Erdiansyah Rahmi Erni Sulistiawati Etih Sudarnika Etty Riani Evalina E Fadli A. Gani Gholib Gholib GONO SEMIADI Gunanti . Hafizuddin Hafizuddin Halim Bakti Harjo Hamny Sofyan Hany Savitry Hellen Kurniati, Hellen Helny Rosita Supriadi Helny Rosita Supriadi Heni Pujiastuti HERA MAHESHWARI Heru Setijanto Heru Setijanto Heru Setijanto Heru Setijanto Huda Salahudin Darusman Huda Shalahudin Darusman I Ketut Mudite Adnyana ICHSAN EFFENDIE Idawati Nasution Idawati Nasution Iman Supriatna Ismahyuningsih, Revita Istiqmal, Kahfi Aulia Ita Djuwita Ita Djuwita Junzo Yamada Ketut Adnyane Mudite Kusdiantoro Mohamad Lidya Elizabeth M. Manik M Agus Setiadi M. Haviz M. Zairin Junior Masaji Washio Md Zuki Abu Bakar Mirnawati Sudarwanto Mokhamad Fahrudin Mokhamad Fakhrul Ulum, Mokhamad MOZES R. TOELIHERE MOZES TOELIHERE MUHAMMAD AGIL Muhammad Jalaluddin Muhammad Risman Wahid Mulyadi Adam MULYOTO PANGESTU Muslim Akmal Nabuo Kitamura Narindria, Yasmin Nadhiva Nastiti Kusumorini Nastiti Kusumorini Nazlie (Alm), Cutnyaâ€™ Shaliran Ni Luh Putu Rischa Phadmacanty Ni Wayan Kurniani Karja Nisa', Chairun Nisa’, Chairun Noordin Mohamed Mustapha Nuraini , Henny Nurhidayat Nurhidayat Nurhidayat Nurhidayat Nurhidayat Odilia Rovara Prawira, Andhika Yudha Putri Syifa Camilla R. Iis Arifiantini Rahma Anisa Razali . RIDWAN AFFANDI Riki Siswandi Ronny Rachman Noor Rusdin - Savitri Novelina Solly Aryza Sri Nurdiati Sri Wahyuni Sri Wahyuni Sriyanto - Supratikno Supratikno . Syahruddin Said TAKDIR SAILI Takdir Syahruddin Said Tongku Nizwan Siregar Trilaksono, Wahyu TUTI L YUSUF Tutik Wresdiyati Tuty L. Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf Ursula Paulawati Maker Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahyuni, Sri Wartika Rosa Farida Wartika Rosa Farida Yanri R.N. Simangunsong Yoshio Yamamoto Yuliani Suparmin

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