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All Journal VALENSI Bionatura Teknotan: Jurnal Industri Teknologi Pertanian Jurnal Natur Indonesia Microbiology Indonesia Majalah Kedokteran Bandung Indonesian Journal of Pharmaceutical Science and Technology LEMMA: Letters Of Mathematics Education Indonesian Journal of Biotechnology QALAMUNA: Jurnal Pendidikan, Sosial, dan Agama Jurnal Biodjati JURNAL PENDIDIKAN JASMANI DAN OLAHRAGA Chimica et Natura Acta Jurnal Moral Kemasyarakatan JURNAL SAINS INDONESIA Jurnal Kimia Terapan Indonesia Jurnal Pendidikan Kimia (JPKim) Indonesian Journal of Chemistry Jurnal PAJAR (Pendidikan dan Pengajaran) Gelanggang Olahraga: Jurnal Pendidikan Jasmani dan Olahraga (JPJO) Molekul: Jurnal Ilmiah Kimia Jurnal Pedagogik Olahraga Journal of Authentic Research on Mathematics Eduacation (JARME) Jurnal PORKES JUMORA: Jurnal Moderasi Olahraga Jurnal Ilmiah Berkala: Sains dan Terapan Kimia Pharmaceutical Sciences and Research (PSR) Rangkiang Mathematics Journal International Journal of Educational Research Excellence (IJERE) Jurnal Sains dan Teknologi Farmasi Indonesia al Kimiya : Jurnal Ilmu Kimia dan Terapan ACTIVE: Journal of Physical Education, Sport, Health and Recreation
Toto Subroto
Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km 21 Jatinangor, Sumedang, 45363 Jawa Barat, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health Social Sciences Other

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Journal : JURNAL SAINS INDONESIA

 1 
Pengaruh Suhu pada Ekspresi Pretrombin-2 Manusia Rekombinan di Escherichia coli ER2566 Silaban, Saronom; Simorangkir, Murniaty; Maksum, Iman Permana; Subroto, Toto; Hasan, Khomaini
Jurnal Sains Indonesia Vol 42, No 1 (2018): Jurnal Sains Indonesia
Publisher : Universitas Negeri Medan

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Abstract

Human recombinant prethrombin-2 (rhPT-2) was expressed in Escherichia coli. Previous studies reported that the expression of rhPT-2 in E. coli tends to formed inclusion body. This study aims to determine the effect of temperature on the expression of rhPT-2 in E. coli ER2566. This study begins with the isolation of recombinant plasmids, competent cell preparation and transformation of competent cells of E. coli ER2566 strain, and rhPT-2 gene expression with various induction temperatures 12, 18, 22 and 30°C. Characterization results showed that the induction temperature of 22°C was the optimum temperature of rhPT-2 gene expression as a soluble fraction in E. coli ER2566 with IPTG concentration 0.1 mM. These results prove that the temperature affects the expression of rhPT-2 in E. coli ER2566.
Co-Authors Abdul Alim Kahari, Abdul Alim Abu Bakar M.I. Syihab Agus Gumilar Alifa I. Nabila Amiruddin, Mohd. Hasril Anita Yuwita Anneke Noviyanti Aprillia, Mutiara Sukma Ari Hardianto Bachti Alisjahbana Basti Andriyoko Bisri Musthofa Budiantoro, Ogi D Agus Yusuf Wildan Dani Permana Debby M Sumanti Defi Supriyati, Ayu DESSY NATALIA DESSY NATALIA DESSY NATALIA Dewi Astriany Dhiya Salsabila Dian Budiana, Dian Diana P Rahmawati Didi Suryadi DWI RAHMAWATI Eddy, Diana Rakhmawaty Effendi, Syulastri EKO YULIANTO Elazmanawati Lembong Fadhilah, Muhammad Naufal Fadhlillah, Muhammad Fadillah, Lukky Farihandiandra, Yusron Fathurrohman, Muhammad Andi Fatin, Nuri Nuraini Fauzian Giansyah Febriani Aji Kusuma, Sherlynda FERNITA PUSPASARI Firdaus, Muhammad Lutfi Gifa Nur Arofah Gumelar, Abih Gumilar, Agus Hafizatunnisa, Hafizatunnisa Hambali, Burhan Hamdiyanti, Meti Henry Chandra Hesti Lina Wiraswati Idar Idar Idar Idar Idar Idar Idar, Idar Iman Permana Maksum Iman Permana Maksum Iman Permana Maksum Iman Permana Maksum Iman Permana Maksum Iman Permana Maksum IMAN PERMANA MAKSUM IMAN PERMANA MAKSUM Iman Permana Maksum Iman Permana Maksum Iman Permana Maksum Kharimah, Nurul Ikhsan KHOMAINI HASAN Kusumawardhani, Shinta Laelasari Laelasari Laelasari, Laelasari Leonardus Wiydatmoko Lesmana, Gianjar Mony, Siti Rasnawati Muchamad Subali Noto Muhammad Fadhil Z, Rusdi Noor Rosa (p:43-50) Muhammad Fadhlillah Muhammad Hilman Daniswara Muhammad Yusuf Muhammad Yusuf Muhammad Yusuf Murniaty Simorangkir Murniaty Simorangkir Naufal D, Kevin Nida Yumna Nisa Fauziah Noviyanti, Anneke Nurfairuz, Hasna Siti Nurmalasari, Ratna Nurul Ikhsan Karimah O Suprijana O Suprijana O Suprijana, O Ogi Budiantoro OGI BUDIANTORO Oo Suprijana Opik Taupiqurrohman Opik Taupiqurrohman Pertiwi, Wulan Praja, Ena Suhena Purba Upay Rahmaniar Mulyani Rahmat, Alit Riezki Amalia Rima Handiyani Rizky Rosjanuardi Rodiana, Ivta Safri Ishmayana Safri Ishmayana Safri Ishmayana Safrl Ismayana Sahroni, Sahroni Santhy Wyantuti Sari Agung, Syennie Sari Syahruni Sari Syahruni SARONOM SILABAN Saronom Silaban Saronom Silaban Saronom Silaban Shabarni Gaffar Shabarni Gaffar Shabarni Gaffar Shabarni Gaffar Shabarni Gaffar Shabarni Gaffar Shabarni Gaffar -, Shabarni Gaffar SHABARNI GHAFFAR Shinta Kusumawardani Siti Soidah Siti Umayah, Siti Soetijoso Soemitro Soetijoso Soemitro Soetijoso Soemitro Soetijoso Soemitro Soetijoso Soemitro Soetijoso Soemitro SOETIJOSO SOEMITRO Sofyan Multazam N Aji Sriwidodo Sriwidodo Sriwidodo Sriwidodo, Sriwidodo Sriwidodo, . Sukma Nuswantara Sukma Nuswantara Sukma Nuswantara Sunan M. Alpiansyah Sutarya Enus Sutarya Enus Sutarya Enus SUTARYA ENUS Syani, Mamay Syfa Nurfadilah Taupiqurrohman, Opik Taupiqurrohman, Opik Tika Pradnjaparamita, Tika Tonah Triana N Meirina Umi Baroroh Unang Supratman Upay, Purba wahyu widayat WANGSA TIRTA ISMAYA Windy Aulia Putri Wulan Pertiwi Yani Suryani Yeni W Hartati Yeni Wahyuni Hartati Zakiyyah, Salma Nur