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Toto Subroto
Departemen Kimia, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Journal : FLUIDA

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Application of Gold Nanoparticles Synthesized with Malva Nut Gum as Reducing Agent for scFv Conjugates in a Colorimetric Chikungunya Virus Detection Muhamad Zulchoir Rasyid; Ratna Yulistya Ningrum; Sinta Setyaningrum; Budi Santoso; Muhammad Yusuf; Bachti Alisjahbana; Toto Subroto; Bevi Lidya
Fluida Vol 16 No 1 (2023): FLUIDA
Publisher : Department of Chemical Engineering, Politeknik Negeri Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35313/fluida.v16i1.4493

Abstract

Gold nanoparticles have good surface plasmon properties in the presence of free electrons on their surface. The presence of free electrons on the surface of the plasmon causes gold nanoparticles to be able to bind to various kinds of biomolecules and proteins, including single-chain variable fragments (scFv). In this study, gold nanoparticle conjugates with Chikungunya antiviral scFv have been successfully prepared with the aim of developing a colorimetric method for rapid detection of envelope protein Chikungunya virus (E2 CHIKV). The manufacture of gold nanoparticles was carried out using malva nut gum and trisodium citrate as reducing agents. The results showed the formation of the AuNP-scFv conjugate which was characterized by a shift in the maximum wavelength for gold nanoparticles synthesized using malva nut gum as a reducing agent from 532.89 nm to 536.62 nm. Similarly, for gold nanoparticles synthesized using trisodium citrate as a reducing agent, the maximum wavelength shifted from 528.99 nm to 531.95 nm. The detection result of E2 CHIKV protein using the AuNP-scFv conjugate has not shown a positive result
Co-Authors Abdul Alim Kahari, Abdul Alim Abu Bakar M.I. Syihab Ade Rizki Ridwan Firdaus Agus Gumilar Ahmad Fariz Maulana Alifa I. Nabila Amiruddin, Mohd. Hasril Angga Himas Setyawan Anita Yuwita Anneke Noviyanti Aprillia, Mutiara Sukma Ari Hardianto Bachti Alisjahbana Bacthi Alisjahbana Basti Andriyoko Bevi Lidya Bevi Lidya Bisri Musthofa Budi Santoso Budiantoro, Ogi Cindy Aulia Sari D Agus Yusuf Wildan Dani Permana Debby M Sumanti Defi Supriyati, Ayu DESSY NATALIA DESSY NATALIA DESSY NATALIA Desy Linasari Dewi Astriany Dewi Ratih Handayani Dhiya Salsabila Dian Budiana, Dian Diana P Rahmawati Diana Rakhmawaty Eddy Didi Suryadi Didin Budiman, Didin DWI RAHMAWATI Dzakiyah 'Izzah Ghassani Adsumi Effendi, Syulastri EKO YULIANTO Elazmanawati Lembong Fadhilah, Muhammad Naufal Fadhlillah, Muhammad Fadillah, Lukky Farihandiandra, Yusron Fathurrohman, Muhammad Andi Fatin, Nuri Nuraini Fauzian Giansyah Febriani Aji Kusuma, Sherlynda FERNITA PUSPASARI Firdaus, Muhammad Lutfi Gifa Nur Arofah Gifa Nur Arofah Gumelar, Abih Gumilar, Agus Hafizatunnisa, Hafizatunnisa Hambali, Burhan Hamdiyanti, Meti Henry Chandra Hesti Lina Wiraswati Idar Idar Idar Idar Idar Idar Idar, Idar Iman Permana Maksum Iman Rahayu Kharimah, Nurul Ikhsan Khomaini Hasan KHOMAINI HASAN Khomairi Hasan Korry Novitriani Korry Novitriani, Korry Kusumawardhani, Shinta Laelasari Laelasari , Laelasari Laelasari Laelasari Laelasari Laelasari, Laelasari Leonardus Wiydatmoko Lesmana, Gianjar Lukky Fadillah Mamlikatu Ilmi Azizah Meti, Meti Hamdiyanti Mony, Siti Rasnawati Muchamad Subali Noto Muhamad Abidin Muhamad Zulchoir Rasyid Muhammad Fadhil Z, Rusdi Noor Rosa (p:43-50) Muhammad Fadhlillah Muhammad Hilman Daniswara Muhammad Yusuf Muhammad Yusuf Muhammad Yusuf Muhammad Yusuf Murniaty Simorangkir Murniaty Simorangkir Naufal D, Kevin Nida Yumna Nisa Fauziah Noviyanti, Anneke Nurfairuz, Hasna Siti Nurmalasari, Ratna Nurul Aida Fathya Nurul Ikhsan Karimah Nurul Ikhsan Karimah O Suprijana O Suprijana O Suprijana, O OGI BUDIANTORO Ogi Budiantoro Oo Suprijana Opik Taupiqurrohman Opik Taupiqurrohman Pertiwi, Wulan Praja, Ena Suhena Purba Upay Rahmaniar Mulyani Rahmaniar Mulyani Rahmat, Alit Ratna Yulistya Ningrum Regi Dwi Septian Riezki Amalia Rima Handiyani Risnina Wafiqoh Rizky Rosjanuardi Rochmat Rahayu Rodiana , Ivta Rodiana, Ivta Safri Ishmayana Safrl Ismayana Sahroni Sahroni Sahroni, Sahroni Santhy Wyantuti Sari Agung, Syennie Sari Syahruni Sari Syahruni SARONOM SILABAN Saronom Silaban Saronom Silaban Saronom Silaban Setyaningrum, Sinta Shabarni Gaffar Shinta Kusumawardani Siti Nuraini Siti Soidah Siti Umayah Siti Umayah, Siti Soetijoso Soemitro Soetijoso Soemitro Soetijoso Soemitro Soetijoso Soemitro Soetijoso Soemitro Soetijoso Soemitro SOETIJOSO SOEMITRO Sofyan Multazam N Aji Sri Asnawati Sri Pitriayana Sriwidodo B, Sriwidodo Sriwidodo Sriwidodo Sriwidodo, . Sukma Nuswantara Sukma Nuswantara Sukma Nuswantara Sunan M. Alpiansyah Surya Amami Pramuditya Sutarya Enus Sutarya Enus Sutarya Enus SUTARYA ENUS Syani, Mamay Syfa Nurfadilah Syfa Nurfadilah Taupiqurrohman, Opik Taupiqurrohman, Opik Tika Pradnjaparamita, Tika Tonah Tonah Triana N Meirina Umi Baroroh Umi Baroroh Unang Supratman Upay, Purba wahyu widayat Wanda Destiarani WANGSA TIRTA ISMAYA Wildatus Sholihah Windy Aulia Putri Wulan Pertiwi Yani Suryani Yeni W Hartati Yeni Wahyuni Hartati Zakiyyah, Salma Nur