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All Journal International Journal of Public Health Science (IJPHS) Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Journal of Food and Pharmaceutical Science Jurnal Ilmiah Farmasi Pharmaciana: Jurnal Kefarmasian Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) Indonesian Journal of Cancer Chemoprevention Berkala Ilmu Kesehatan Kulit dan Kelamin Folia Medica Indonesiana Majalah Obat Tradisional JOURNAL OF HEALTHCARE TECHNOLOGY AND MEDICINE MPI (Media Pharmaceutica Indonesiana) INDONESIAN JOURNAL OF PHARMACY JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Jurnal Farmasi Sains dan Komunitas Jurnal Surya Medika Farmasains : Jurnal Ilmiah Ilmu Kefarmasian Jurnal Biologi Tropis Majalah Farmaseutik Media Farmasi PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Jurnal Farmasi Indonesia Jurnal Ilmu Kefarmasian Indonesia JURNAL FARMASIMED (JFM) Jurnal Abdimas Mahakam CERATA Jurnal Ilmu Farmasi SPEKTA (Jurnal Pengabdian Kepada Masyarakat : Teknologi dan Aplikasi) Jurnal Insan Farmasi Indonesia SINTA Journal (Science, Technology, and Agricultural) Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Pharmaceutical Sciences and Research (PSR) Journal of Halal Science and Research Sciences of Pharmacy Jurnal Farmasetis Jurnal Penelitian dan Kajian Ilmiah Kesehatan Politeknik Medica Farma Husada Mataram Journal of Food and Pharmaceutical Sciences CERATA Medical Sains : Jurnal Ilmiah Kefarmasian E-Amal: Jurnal Pengabdian Kepada Masyarakat Indonesian Journal of Jamu

Nining Sugihartini

Fakultas Farmasi, Universitas Ahmad Dahlan, Yogyakarta, Indonesia

Author-ID : 388808

Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

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Daya Antioksidan Ekstrak Kulit Buah Naga Dan Sabun Ekstrak Kulit Buah Naga (Hylocereus Polyrhizuz) Mohamad Ikram; Nining Sugihartini; Any Guntarti
Jurnal Surya Medika (JSM) Vol 6 No 1 (2020): Jurnal Surya Medika (JSM)
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/jsm.v6i1.1616

Red dragon fruit peel extract contains betalain which has antioxidant activity. In addition, this extract also contains dyes that can be used as coloring agents in cosmetic preparations. The purpose of this study was to study the activity of dragon fruit peel extract and red dragon fruit peel extract soap with various concentrations. Antioxidant activity is taken by the DPPH method. The concentration of red dragon fruit peel extract in soap is 0.3; 0.6; 0.9%. Test results show red dragon fruit extract soap has lower antioxidant activity than the extract. The increased concentration of the extract caused an increase in the antioxidant activity of the red dragon fruit peel extract soap.

EVALUASI SIFAT FISIK DAN UJI IRITASI SEDIAAN SALEP MINYAK ATSIRI BUNGA CENGKEH DALAM BASIS LARUT AIR Diah Pratimasari; Nining Sugihartini; Tedjo Yuwono
Jurnal Ilmiah Farmasi Vol. 11 No. 1 (2015): Jurnal Ilmiah Farmasi
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/jif.vol11.iss1.art2

Essential oil of clove (Syzygium aromaticum) (MABC) with eugenol as its main active ingredient, has been studied as anti-inflammatory agent. Its important to develop formulation in an advanced research. This study has been conducted to develop a topical dosage form (an ointment using a water-soluble base). The physical properties of water-soluble ointment preparation of MABC and the irritation properties at various concentrations of MABC have been evaluated. Ointment preparation MABC water-soluble base created using the mixing of the PEG 400 and PEG 4000 by a fusion method. Ointment made with a various concentration of MABC (5%, 10% and 15%). Ointment with each concentration were evaluated based on its physical properties such as pH, dispersiveness and adhesion. Ointment also tested its irritative properties on the skin, using test animals guinea pigs by Draize test method. Data from those experiments analyzed by ANOVA with T-test level of 95%. The results showed that the physical properties of water-soluble ointment base with MABC concentration of 5%, 10% and 15% reach the requirements of the pH and adhesion parameter, but not for its dispersiveness. Statistical analysis of each trial did not show any significant in difference. The experimental results against irritation test showed that the water-soluble ointment base concentration of 5%, 10% and 15% did not cause irritation to the skin of guinea pigs.

SIFAT FISIK DAN DAYA IRITASI KRIM TIPE A/M MINYAK ATSIRI BUNGA CENGKEH (Syzigium aromaticum) DENGAN BERBAGAI VARIASI KONSENTRASI Erma Pranawati; Nining Sugihartini; Tedjo Yuwono
Jurnal Ilmiah Farmasi Vol. 12 No. 1 (2016): Jurnal Ilmiah Farmasi
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract Eugenol contained in the essential oil of clove (Syzygium aromaticum) has pharmacological effects as anti-inflammatory agent. This study aims to develop a topical dosage forms such as creams with type A / M. One important factor in the development of formulations is the determination of the concentration of the active substance. The purpose of this study was to determine the effect of the concentration of essential oil of clove preparations cream type M / A in the physical and its irritative effect. Cream type A / M contained clove essential oil is made by melting method with concentration of clove essential oil : 5%, 10%, and 15%. Evaluation cream type A / M includes the physical properties of scattered power, adhesion, and pH. The irritation test also conducted using test animals guinea pigs with Remington method. Data obtained from the experiment were analyzed using ANOVA test continued statistical t-test with a level of 95%. Physical properties test results indicate that increased concentrations of clove essential oils in the cream type A / M led to an increase in adhesion (p <0.05), the scatter (p <0.05) and pH (p> 0.05). The experimental results showed that the irritation test of the type A / M cream with a concentration of 5%, 10% and 15% of clove essential oil does not cause irritation to the skin of guinea pigs. Keywords: Eugenol, cream, irritation index, physical properties Intisari Eugenol yang terkandung dalam minyak atsiri bunga cengkeh (Syzygium aromaticum) memiliki efek farmakologi sebagai antiinflamasi. Penelitian ini merupakan penelitian lanjutan untuk mengembangkan bentuk sediaan topikal berupa krim dengan tipe A/M. Salah satu faktor penting dalam pengembangan formulasi adalah penentuan konsentrasi zat aktif. Tujuan penelitian ini adalah mengetahui pengaruh konsentrasi minyak atsiri bunga cengkeh sediaan krim tipe M/A terhadap sifat fisik dan daya iritasinya.Krim tipe A/M minyak atsiri bunga cengkeh dibuat dengan metode peleburan dengan konsentrasi minyak atsiri bunga cengkeh yaitu 5%, 10%, dan 15%. Evaluasi krim tipe A/M meliputi uji sifat fisik yaitu uji daya sebar, daya lekat, dan pH. Selain itu juga dilakukan uji iritasi dilakukan menggunakan hewan uji marmut dengan metode Remington data yang diperoleh dari percobaan dianalisis dengan statistika menggunakan uji ANOVA dilanjut uji-t dengan taraf kepercayaan 95%. Hasil uji sifat fisik menunjukkan bahwa peningkatan konsentrasi minyak atsiri bunga cengkeh dalam krim tipe A/M menyebabkan peningkatan daya lekat (p<0,05), daya sebar (p<0,05) dan pH (p>0,05). Hasil percobaan uji iritasi menunjukkan bahwa krim tipe A/M dengan konsentrasi 5%, 10% dan 15% tidak menimbulkan iritasi pada kulit marmut. Kata kunci : Eugenol, Krim, Indeks iritasi, Sifat fisik

Pengaruh Purifikasi Terhadap Kandungan Zat Aktif Dan Aktivitas Antioksidan Ekstrak Etanol 50% Daun Kelor (Moringa oleifera L.) Diyan Sakti Purwanto; Hari Susanti; Nining Sugihartini
CERATA Jurnal Ilmu Farmasi Vol 12 No 2 (2021): Cerata Jurnal Ilmu Farmasi
Publisher : STIKES MUHAMMADIYAH KLATEN

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Purifikasi ekstrak etanol 50% daun kelor (Moringa oleifera L.) dilakukan untuk meningkatkan kandungan zat aktif flavanoid, β-karoten, saponin dan tanin yang memiliki aktivitas sebagai antioksidan. Tujuan penelitian ini adalah mengetahui jenis pelarut yang paling optimal antara etil asetat dan n-heksan dalam fraksinasi ekstrak etanol 50% daun kelor. Penelitian dimulai ekstraksi etanol 50% daun kelor dengan maserasi dan dilanjutkan evaporator kemudian waterbath sampai diperoleh ekstrak kental. Ekstrak kental yang diperoleh dari ekstraksi selanjutnya dilakukan purifikasi. Fraksinasi dengan melarutkan ekstrak kental etanol 50% daun kelor (E1) dan aquades pada suhu 70ºC kemudian ditambahkan etil asetat pada corong pisah hingga di peroleh fraksi etil asetat (E2), ekstrak kental etanol 50% daun kelor (E1) ditambahkan n-heksan pada corong pisah hingga terjadi pemisahan fase sehingga diperoleh fraksi n-heksana (E3). Ekstrak dan fraksi dievaluasi parameter meliputi kadar air ekstrak dengan gravimetri, kadar β-karoten dengan HPLC dan aktivitas antioksidan dengan DPPH. Data dianalisis statistik dengan taraf kepercayaam 95%. Hasil Penelitian menunjukkan bahwa kadar air ekstrak etanol 50% daun kelor adalah 4,16 dengan CV 4,62%. Kadar β-karoten paling optimal pada fraksi n-heksana (E3) kemudian etil asetat (E2) dan etanol 50% (E1) yaitu 1.48 ± 0.01%, 1.19 ± 005% dan 0.73 ± 0.01%. Sedangkan aktivitas antioksidan (IC50) paling optimal pada fraksi n-heksana (E3) kemudian etanol 50% (E1) dan etil asetat (E2) yaitu 40.83 ± 0.04 ug/ml, 47.75 ± 0.09 ug/ml dan 58.79 ± 0.10ug/ml. Nilai aktivitas antioksian menunjukkan pada fraksi n-heksana dan ekstrak etanol 50% termasuk dalam kategori sangat kuat sedangkan fraksi etil asetat termasuk dalam kategori kuat sehingga berpotensi sebagai antioksidan.

Determination of Total Phenolic Levels in Ethanol Extract of Moringa (Moringa oleifera L.) Leaves based on Differences in Growing Sites Any Guntarti; Nining Sugihartini; Siti Athiyah Umaiyah; Nina Salamah
Journal of Food and Pharmaceutical Sciences Vol 9, No 1 (2021): J.Food.Pharm.Sci
Publisher : Institute for Halal Industry and System (IHIS) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.1337

Moringa oleifera L. have good nutritional content including phenolic compounds which can be used as antioxidants and can grow in lowlands and highlands. The purpose of this study was to determine the total phenolic content of the ethanol extract of M. oleifera leaves with variations in the area of collection. The 50% ethanol extract was obtained from the simplicia of M. oleifera leaves by using the maceration method. Analysis of total phenolic content in the extract was carried out using a spectrophotometer instrument with the addition of reagent Folin-Ciocalteu and gallic acid as standard. The results of total phenolic content in Sleman, Wonosari, and Wonosobo areas were (127.87 ± 2.71) mg GAE / g extract, (99.40 ± 2.68) mg GAE / g extract, and (142 , 92 ± 1.81) mg GAE / g extract. The highest phenolic content in the ethanol extract of moringa leaves was found in Wonosobo areas.

VALIDASI METODE ANALISA PENETAPAN KADAR EPIGALOKATEKIN GALAT DENGAN KROMATOGRAFI CAIR KINERJA TINGGI Nining Sugihartini; Achmad Fudholi; Suwidjiyo Pramono; Sismindari Sismindari
Pharmaciana Vol 4, No 2 (2014): Pharmaciana
Publisher : Universitas Ahmad Dahlan

High Performance Liquid Chromatography (HPLC) was one of analytical methodscommonly used to determine the concentration of epigallocatechin gallate (EGCG) on green teaextract. The method must be validated in order to fit to its purpose. The aim of this research was toprove that the used method has selectifity, liniearity, precise, accurate and know limit of detection(LOD) and limit of quantification (LOQ) is acceptable. The selectivity of analytical method wasdetermined by calculating the resolution value between two peak. Data from 10 Î¼g/mL and100 Î¼g/mL with 5 replicates would give precition and accuration. Precition was known from CV value and accuration was known from recovery value in each concentration. Liniearity was knownfrom regression linear between concentration and wide area of peak. From regresion linear couldcalculate LOD and LOQ. Research show that method of analyse have selectificity withRs= 2.27>1.5; liniearity with r= 0.99; precision with CV 8.74% at concentration 200 Âµg/mL and3.74% at concentration 500 Âµg/mL; accuration with recovery 99.76% at concentration 200 Âµg/mLand 100.52% at concentration 500 Âµg/mL and the value of LOD is 33.28 Î¼g/mL and LOQ is110.93 Î¼g/mL.

Antibacterial activity of dialkyl-aginate biosurfactant cream againts Staphylococcus aureus an Pseudomonas aerugynosa Kintoko Kintoko; Nining Sugihartini; Benazir Evita Rukaya
Pharmaciana Vol 8, No 2 (2018): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Dialkyl-alginate biosurfactant is an amphifilik rhamnolipid biosurfactant that has the potential to be developed into an antibacterial agent. The purpose of this study was to prove that the biosurfactant of dialkyl alginate both before and after creams has antibacterial activity especially against Staphylococcus aureus and Pseudomonas aeruginosa. Test of antibacterial activity of biosurfactant dialkyl alginate at concentrations of 5%, 10% and 20% to Staphylococcus aureus and Pseudomonas aeruginosa using Hammond, et al (2011) modified quantitative method. EnbaticÂ® 1% is used as a positive control and sterile aquadest as a negative control. Determination of antibacterial activity of dialkyl alginate biosurfactant followed by analysis of leakage of protein and nucleic acids using UV-vis Spectrophotometry and leakage of Ca2 + and K + metal ions using Atomic Absorption Spetrophotometry (AAS). The most active concentration was formulated into cream and then performed physical evaluation (organoleptic, pH, adhesion, spreading and viscosity) and tested antibacterial activity using the well method. The test results showed that the biosurfactants of dialkyl-alginate before and after the cream was treated as antibacterial activity. The concentration of 10% was the most active concentration having activity which did not differ significantly to positive control with p value of 0,05

EVALUASI UJI IRITASI DAN UJI SIFAT FISIK SEDIAAN EMULGEL MINYAK ATSIRI BUNGA CENGKEH (Syzigium aromaticum) Delia Komala Sari; Nining Sugihartini; Tedjo Yuwono
Pharmaciana Vol 5, No 2 (2015): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Inflammation is the human body's response to a tissue damage as indicated by an increase of vascular permeability. One of the natural substances that can be used to treat inflammation is clove oil (MABC). This study aimed to evaluate the physical properties and MABC emulgel irritation test (Syzygium aromaticum). This experimental study was initiated by the emulgel formulation with three kinds of formula, with each formula contains MABC levels of 10% (F1), 12.5% (F2), and 15% (F3). The physical tests for emulgel was done including pH, spreading test and adhesive test and irritation test in guinea pigs using Draize test method. Statistical analysis by t test with a level of confidence 95% was conducted to detect a significant differences between the treatment groups. The emulgel formula with a concentration of 10%, 12.5% and 15% were physically stable, moreover the dispersive power test for each formula showed normal distribution data (p>0.05) and homogeneous (p>0.05), ANOVA test results obtained significantly different results in each formula (p<0.05), whereas the stickiness test results for each formula more than 30 minutes with pH 6. The results of irritation test by using Draize test Â method, indicated that each formula did not show any irritant effect.

VALIDASI METODE ANALISA PENETAPAN KADAR EPIGALOKATEKIN GALAT DENGAN KROMATOGRAFI CAIR KINERJA TINGGI Nining Sugihartini; Achmad Fudholi; Suwidjiyo Pramono; Sismindari Sismindari
Pharmaciana Vol 4, No 1 (2014): Pharmaciana
Publisher : Universitas Ahmad Dahlan

High Performance Liquid Chromatography (HPLC) was one of analytical methods commonlyto determine the concentration of epigallocatechin gallate (EGCG) on green tea extract. The methodmust be validated in order to fit to its purpose. The aim of this research was to prove that the usedmethod has selectifity, liniearity, precise, accurate and know limit of detection (LOD) and limit ofquantification (LOQ) is acceptable. The selectifity of analytical method was determined bycalculating the resolution value between two peak. Data from 10 ug/ml and 100 ug/ml with 5replicates would give precition and accuration. Precition was known from CV value and accurationwas known from recovery value in each concentration. Liniearity was known from regression linearbetween concentration and wide area of peak. From regresion linear could calculate LOD and LOQ.Research show that method of analyse have selectificity with Rs= 2,27>1,5; liniearity with r= 0,99;precision with CV 8,74% at concentration 200 Âµg/ml and 3,74% at concentration 500 Âµg/ml;accuration with recovery 99,76% at concentration 200 Âµg/ml and 100,52% at concentration 500 Âµg/mland the value of LOD is 33,28 ug/ml and LOQ is 110,93 ug/ml.

FORMULASI GEL EKSTRAK ETANOL KULIT BUAH MANGGIS (Garcinia mangostana L.) DENGAN VARIASI GELLING AGENT SEBAGAI SEDIAAN LUKA BAKAR Lena Maulina; Nining Sugihartini
Pharmaciana Vol 5, No 1 (2015): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Pericarp of mangosteen is waste that has been shown have efficacy as an anti-inflammatory and accelerate the proliferation of fibroblasts. Therefore Â it need to be formulated in the form of gel to make easy in the use of preparation. It is needed an appropriate gelling agent to produce the good character of gel. This research aimed to know the type of the gelling agent who has the physical properties gel and the activity of the best treat burns. There are three gel formula designed with various Â type ofÂ the gelling agent i.e. FI (the gelling agent carbopol); FII (the gelling agent CMC na); FIII (the gelling agent tragacanth). The obtained gels were tested organoleptic, of homogeneity, pH, spreadibility, adhesivity, consistency and the effect of healing burns in nine groups. The collected data were analyzed statistically using SPSS version 16 with kolmogorov-smirnov and continued one way ANOVA or Â Kruskal-Wallis, with level of trust 95 %. The results show that all formula mixed homogeneous gel. Formula I have a pH 3,5 while formula II and III having a ph 5 (p < 0,05). The greater spreadibility given by formula III (p < 0,05) and the longest time of adhesivity given by formula II (p < 0,05). The best activity of wound healing given by formula I (p > 0,05). Based on the result show that type of gelling agent can influence the physical properties and activity in the treating burns. Type of gelling agent that show the best physical properties is CMC Na.

Co-Authors . Sismindari Achmad Fudholi Achmad Fudholi Achmad Fudholi Achmad Fudholi Aditya Noviadi Rakhmatullah Afriyani, Neti Agung Putu S.P.Kristyawan Aina Fatkhil Haque, Aina Fatkhil Aji Pamungkas Alvionida, Fitra Amraini Amelia Ani Guntarti Any Guntarti Any Guntarti Astryna, Syarifah Yanti Atri Sri Ulandari Auliah Rahmi Latif Azis Ikhsanudin Bayu Herdi Al Huda Deasy Vanda Pertiwi Delia Komala Sari, Delia Komala Desrianti, Cindy Desyandri Desyandri Diah Pratimasari Diyan Sakti Purwanto Diyan Sakti Purwanto Endah Nurlaela Endah Nurlaela Endang Lukitaningsih Erma Pranawati ERMAWATI, DIAN EKA Erna Astuti Erna Astuti Erwin Fauzana Evi Nuryanti Fadzil Latifah Firsty, Girly Risma Fitra Alvionida Fitri Rahmawanti Fitriah Ardiawijianti Iriani Fudholi, Achmad Fudholi, Achmad Gina Lestari Gina Lestari, Gina Girly Risma Firsty Gita Puspita Hafid, Indah Fitria Hana Hanifah Hari Susanti Hari Susanti Heru Sasongko Ichwan Ridwan Rais Iis Wahyuningsih Jannah, Syauqul Kintoko Kintoko Kintoko, Kintoko Kurniawan, Muhammad Fariez Kusuma, Nyoman Rudi Laela Hayu Nurani Laila, Wahyu Kumil Lalu Muhammad Irham Lela Sulastri, Lela Lena Maulina Lena Maulina, Lena M Alif Fajri Marchaban . Miratun Syarifah Mohamad Ikram Muhammad Alfian, Muhammad Muhammad Fariez Kurniawan Muhammad Fariez Kurniawan Muhammad Yusron Maulana El-Yunusi Mukhlishah, Neneng Rachmalia Izzatul Nanik Sulistyani Nina Salamah NINGRUM, TYAS SARI RATNA Novitasari, Putri Rachma Nugrahini, Febrina Nurhayati Nurhayati Nuri Ari Efiana Nurlaela, Endah Olvia Becatami Widyantoro Olvia Becatami Widyantoro, Olvia Becatami Pertiwi, Deasy Vanda Pramono, Suwidjiyo Pramono, Suwidjiyo Puput Herawati Said Hasan Rahmat Hidayat Rahmawati, Desty Restia Raka Sukmabayu W.S Ratih Saridewi Rikadyanti Rikadyanti Rinanda Yeshi Wiradhika Ririn Suharsanti, Ririn Ristiono, Hendi Rosmiyanti, Yanti Rukaya, Benazir Evita Ryan Radix Rahadhian, Muhammad Sapto Yuliani Sapto Yuliani Sapto Yuliani Sismindari . Sismindari Sismindari Sismindari Sismindari Sismindari Sismindari Sismindari, Sismindari Sismindari, Sismindari Siti Athiyah Umaiyah Siti Ayu Marlina Sri Mulyaningsih Sri Mulyaningsih Srimuliani Arbie Suwidjiyo Pramono Suwidjiyo Pramono Suwidjiyo Pramono Suwidjiyo Pramono Suwidjiyo Pramono. Tedjo Yuwono Tedjo Yuwono Tedjo Yuwono Tedjo Yuwono Tedjo Yuwono Tedjo Yuwono Tedjo Yuwono Tedjo Yuwono Teuku Nanda Saifullah, Teuku Nanda Trecya Fujiastuti Trecya Fujiastuti, Trecya Tusilowati, Defita Andri Ulfa Ramdhani M Uswatun Chasanah Vivi Sophia Waliyyin Razan Qanit wardhani, Riska risty Widyantoro, Olvia Becatami Widyasari Putranti Wisnu Kundarto Wuryandari, Tutik Wuryandari, Tutik Yayan Rizikiyan Zaenab Zaenab Zainab Zainab Zainab Zainab Zainab

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