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All Journal International Journal of Public Health Science (IJPHS) Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Journal of Food and Pharmaceutical Science Jurnal Ilmiah Farmasi Pharmaciana: Jurnal Kefarmasian Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) Indonesian Journal of Cancer Chemoprevention Berkala Ilmu Kesehatan Kulit dan Kelamin Folia Medica Indonesiana Majalah Obat Tradisional JOURNAL OF HEALTHCARE TECHNOLOGY AND MEDICINE MPI (Media Pharmaceutica Indonesiana) INDONESIAN JOURNAL OF PHARMACY JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Jurnal Farmasi Sains dan Komunitas Jurnal Surya Medika Farmasains : Jurnal Ilmiah Ilmu Kefarmasian Jurnal Biologi Tropis Majalah Farmaseutik Media Farmasi PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Jurnal Farmasi Indonesia Jurnal Ilmu Kefarmasian Indonesia JURNAL FARMASIMED (JFM) Jurnal Abdimas Mahakam CERATA Jurnal Ilmu Farmasi SPEKTA (Jurnal Pengabdian Kepada Masyarakat : Teknologi dan Aplikasi) Jurnal Insan Farmasi Indonesia SINTA Journal (Science, Technology, and Agricultural) Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Pharmaceutical Sciences and Research (PSR) Journal of Halal Science and Research Sciences of Pharmacy Jurnal Farmasetis Jurnal Penelitian dan Kajian Ilmiah Kesehatan Politeknik Medica Farma Husada Mataram Journal of Food and Pharmaceutical Sciences CERATA Medical Sains : Jurnal Ilmiah Kefarmasian E-Amal: Jurnal Pengabdian Kepada Masyarakat Indonesian Journal of Jamu

Nining Sugihartini

Fakultas Farmasi, Universitas Ahmad Dahlan, Yogyakarta, Indonesia

Author-ID : 388808

Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

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OPTIMASI KOMPOSISI TEPUNG BERAS DAN FRAKSI ETANOL DAUN SENDOK (Plantago major, L) DALAM FORMULASI TABIR SURYA DENGAN METODE SIMPLEX LATTICE DESIGN Nining Sugihartini
Pharmaciana Vol 1, No 2 (2011): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Octyl methoxycinnamate is an active ingredient in sunscreen that will bedegradated after exposure by sunlight. On the other hand, antioxidants have potentialcapability as photoprotector which in this study is used the ethanol fraction of leaf ofPlantago major, L to support activity of OMS. In addition, rice flour is added toobecause it has the ability to reflect sunlight. The aim of this study is to obtain theoptimum composition of them, based on the effectivity of OMS. This study used threeformulas based on the simplex lattice design method with a comparison of octylmethoxycinnamate and rice flour as follows: FI (100%: 0%), the formula II (50%:50%), formula III (0%: 100%). All formulas were exposured by sunlight for 5 hours (at09:00 to 14:00 GMT) and in interval one hour samples were taken to measure the valueof % erythema transmission (% Te) and pigmentation (% Tp). Data were analysed toget the optimum composition which has the highest of response. The results showed that100% rice flour was the optimum addition to support activity of OMS as sunscreen withdecreasing of % erythema transmission and pigmentation was 200%, 75%,respectively.

Anti-inflammatory activity of essential oil of clove (Syzygium aromaticum) in O/W and W/O Creams Nining Sugihartini; Gina Lestari; Sapto Yuliani
Pharmaciana Vol 9, No 1 (2019): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Previous studies have shown that the formulations of clove bud essential oil (CBEO) with a concentration of 5% in O/W cream and 2.5% in W/O cream have anti-inflammatory properties. The formulations are developed by adding enhancers, namely oleic acid and propylene glycol. The optimum compositions of oleic acid and propylene glycol are 50%:50% in W/O cream and 30%:70% in O/W cream. This study aimed to determine the effects of different compositions of creams on their anti-inflammatory activities. The creams were evaluated for their anti-inflammatory properties using mice with croton oil-induced inflammatory. The mice were sacrificed, and the back skin was removed to make histopathologic preparations at the end of the three-day inflammatory induction to obtain data of epidermal thickness and the number of cells with COX-2 expression. The results showed that the addition of oleic acid and propylene glycol as enhancers increased the anti-inflammatory activity of both O/W and W/O creams. Since the O/W cream decreased the thickness of the epidermis and the number of cells with COX-2 expression more than the W/O cream, the formulation of CBEO in O/W cream is concluded as a better anti-inflammatory than that of W/O cream.Â Â Â

Cox-2 inhibition activities of creams containing anguilla bicolor and sea cucumbers extract on croton oil induced inflammation in mice Heru Sasongko; Waliyyin Razan Qanit; Raka Sukmabayu W.S; Agung Putu S.P.Kristyawan; Nining Sugihartini; Wisnu Kundarto; Dian Eka Ermawati
Pharmaciana Vol 10, No 3 (2020): Pharmaciana
Publisher : Universitas Ahmad Dahlan

The fatty acids, like EPA and DHA, were known as anti-inflammation. It works on inflammatory tissue, brought by edema plasma, and changed into resolvins, protectins, and maresins by the enzyme reaction. Anguilla bicolor and Sea cucumber fish are known to contain EPA and DHA. This study analyzes the inhibition Cox-2 effect of the combination of Anguilla bicolor oil with Sea cucumber extract as an active ingredient in a cream preparation. This experiment used 7 groups of male BALB/C strain mice: normal control; negative control; positive control; A. bicolor oil cream; H. leucospilota extract cream; a combination of A. bicolor : H. leucospilota (2:1) cream; a combination of A. bicolor : H. leucospilota (1:2) cream. The anti-inflammatory activity was evaluated by the amount of inflammatory cell, the thickness epidermis, and the amount of cell expression COX-2Â on mice's back skin tissue induced by croton oil (0,1 %). After 3 days, histopathological skin tissues were made. The data were analyzed statistically by One Way ANOVA followed by LSD to know each group's differences at a significance level of 0.05. The experiment results showed that the formula with the best anti-inflammatory activities is the combination of A. bicolor and H. leucospilota (2:1) cream.Â The decrease of the amount of inflammatory cell (75.97%), the thickness of the epidermis (43.88%), and the amount of COX-2 cell expressions (60.52%) from the formula did not differ significantly with the positive control (p>0,05). It can be concluded that A. bicolor and H. leucospilota have anti-inflammation activity based on the experiment results.

VALIDASI METODE ANALISA PENETAPAN KADAR EPIGALOKATEKIN GALAT DENGAN KLT DENSITOMETRI Nining Sugihartini; Achmad Fudholi; Suwidjiyo Pramono; Sismindari Sismindari
Pharmaciana Vol 2, No 1 (2012): Pharmaciana
Publisher : Universitas Ahmad Dahlan

TLC Densitometry is one of method which was used to measure amount of activesubstance. Active substance of epigallocathechin gallate is one of in green tea extractcream. Method of analysis must be validated to prove that method will give the dataclose with the real value. Aim of this research is to prove that TLC Densitometry methodhave liniearity, precision and accuration that fulfill the requirement. This study usedconcentration 600, 1800 and 3000 ug/ml with 3 replications to measure precision(based on value of CV) and accuration (based on value of recovery). Liniearity wasknown based on value of r of curve regression between concentration and wide area ofchromatogram. LOD and LOQ was calculated based on curve regression. Researchshowed that method of analyse had liniearity with r= 0,98. Value of CV concentrationof 600, 1800 and 3000 ug/ml were 8,18%; 3,19% and 1,53%, respectively and recoverywere 88,10%; 99,65% dan 111,33%, respectively. Value of LOD was 827,01 Âµg/ml andLOQ was 2756,69 Âµg/ml.

OPTIMASI KOMPOSISI TWEEN 80 DAN SPAN 80 SEBAGAI EMULGATOR DALAM REPELAN MINYAK ATSIRI DAUN SERE (Cymbopogon citratus (DC) Stapf) TERHADAP NYAMUK Aedes aegypti BETINA PADA BASIS VANISHING CREAM DENGAN METODE SIMPLEX LATTICE DESIGN Endah Nurlaela; Nining Sugihartini; Azis Ikhsanudin
Pharmaciana Vol 2, No 1 (2012): Pharmaciana
Publisher : Universitas Ahmad Dahlan

The disease of Dengue hemorrhagic fever (DHF) is caused by the bite of femaleAedes aegypti mosquito. Cream of repelan sere leaf essential oils can be used to preventmosquito bites. The cream of volatile oil sere (Cymbopogon citratus (DC) Stapf)required emulsifier to maintain the stability of cream. Tween 80 and Span 80 is a widelyused emulsifier in a cream formula. Aims of this study is to determine the optimumcomposition of Tween 80 and Span 80 as emulsifier in a cream repelan of sere leafessential oils in the basis Vanishing Cream with Simplex Lattice Design methode. ThreeFormula cream is designed based on the Simplex Lattice Design methode with a ratio ofTween 80: Span 80 as follows: FI (100%-0%), F II (50%-50%), F III (0%-100%).Cream was then tested repelan activity and physical properties that includespreadibility, adhesivity and irritation. Data of physical properties and repellantactivity was calculate to get the optimum formula. The validity of optimization methodswas evaluated by comparing experimental results with predicted results in optimumcream formula with t test at 95% level of confidence. The results of study showed that theoptimum composition of Tween 80: Span 80 was 60%:40%. Cream with optimumemulsifier composition was predicted has spreadibility 29.24 cm2; adhesivity 3.512seconds; irritation 0.608; power repelan 41.469 minutes. T test results showed nosignificant differences between the experimental results and predicted results (p<0.05).

Composition of carbopol 940 and HPMC affects antibacterial activity of beluntas (Pluchea indica (L.)) leaves extract gel Fitra Alvionida; Nanik Sulistyani; Nining Sugihartini
Pharmaciana Vol 11, No 3 (2021): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Indonesia is a country known for its source of biological wealth, one of which is beluntas leaves. Beluntas leaves have the potential to be an antibacterial, so it is appropriate to be formulated in the form of medicinal preparations, especially gels. This study aims to find out the influence of variations between carbopol gel base 940 and Methylcellulose Hydroxypropyl (HPMC) on the physical properties of gel preparations beluntas leaf extract (Pluchea indica (L.), and know the influence of gel of extract of beluntas leaves on antibacterial activity. The extract is obtained by the maceration method using ethanol solvent 96%. Each formula uses 15% of the extract of beluntas leaves. Gels are made in four gel base variations namely F0 (0.5% carbopol, 1% HPMC), FI (1% carbopol, 1.5% HPMC), FII (1.5% carbopol, 2.5% HPMC), and FIII (2% carbopol, 3% HPMC). Gels evaluated for their physical properties include organoleptic, viscosity, pH, homogeneity, scattering power, adhesion and Freeze-thaw cycling. Then the gel tested antibacterial activity against bacteria Staphylococcus aureus and Pseudomonas aeruginosa by cup-plate diffusion method. The data obtained were analyzed with One Way Anova and LSD with a 95% confidence level. The results showed that beluntas leaf extract gel meets the organoleptic requirements, homogeneity, good gel adhesion (> 4sec), good gel viscosity (2000-50.000 cps), and good gel pH (4.5-6.5). However, the gel does not meet the requirements of good scattering power (5-7 cm) and Freeze-thaw cycling. Based on the test results that have been done with some of the parameters above, The best composition of carbopol 940 and HPMC in the beluntas leaf extract gel which has antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa Â is 1% carbopol and 1.5% HPMC. The antibacterial activity of the formula is categorized as strong.

FORMULASI DAN KARAKTERISASI SEDIAAN HIDROGEL MINYAK CENGKEH (Syzygium aromaticum) BERBASIS KITOSAN Deasy Vanda Pertiwi; Azis Ikhsanudin; Nining Sugihartini
Media Farmasi: Jurnal Ilmu Farmasi Vol 14, No 1: Maret 2017
Publisher : Universitas Ahmad Dahlan

Eugenol merupakan kandungan utama minyak cengkeh yang memiliki potensi sebagai anti inflamasi. Bentuk sediaan yang berupa cairan berminyak menimbulkan ketidaknyamanan dan tidak bertahan lama untuk kontak dengan kulit sehingga efikasi yang diharapkan tidak tercapai jika digunakan langsung pada permukaan kulit. Oleh karena itu, dibutuhkan sistem sediaan yang dapat menghantarkan obat menembus kulit dengan lebih baik. Hidrogel adalah sediaan semipadat yang diharapkan dapatÂ diaplikasikan sebagai pembawa minyak cengkeh, sehingga akan meningkatkan kenyamanan dan efikasinya. Pembuatan hidrogel minyak cengkeh dilakukan dengan memformulasikan minyak cengkeh, surfaktan tween 80 dan propilen glikol (PG) menjadi emulsi. Desain formula dibuat menggunakan Design Expert 10 trial. Formula emulsi yang optimum selanjutnya diformulasikan dengan basis gel kitosan konsentrasi 4% dan 5%. Hidrogel minyak cengkeh diformulasikan menjadi emulsi yang selanjutnya ditambahkan ke dalam basis gel berupa kitosan. Dari hasil uji karakterisasi gel diperoleh bentuk emulgel berwarna putih kekuningan dengan pH 6. Uji daya sebar menunjukkan formula 1 dengan basis kitosan 5% memiliki daya sebar yang paling baik, yaitu 5,1 cm. Hasil uji daya lekat menunjukkan bahwa keempat formula memenuhi syarat uji daya lekat, yaitu tidak kurang dari 4 detik. Dari uji karakteristik, formula F1 5% merupakan formula yang optimal karena memenuhi semua persyaratan sifat fisik. Hal tersebut menunjukkan bahwa minyak cengkeh dapat menghasilkan sediaan gel yang baik dengan basis kitosan.

Uji Sifat Fisik Dan Aktivitas Ekstrak Daun Petai Cina (Leucaena Glauca, Benth) Dalam Berbagai Tipe Basis Salep Sebagai Obat Luka Bakar Olvia Becatami Widyantoro; Nining Sugihartini
Media Farmasi: Jurnal Ilmu Farmasi Vol 12, No 2: September 2015
Publisher : Universitas Ahmad Dahlan

Luka bakar merupakan kerusakan jaringan yang dapat disebabkan kontak dengan sumber panas. Daun petai cina mengandung senyawa aktif saponin, alkaloid, flavonoid dan tanin yang mempunyai aktivitas sebagai penyembuh luka bakar. Penelitian ini bertujuan mengetahui pengaruh tipe basis salep terhadap aktivitas penyembuhan luka bakar dan sifat fisiknya. Ekstrak daun petai cina diperoleh dengan metode maserasi menggunakan etanol 70%. Ekstrak dibuat salep dengan basis salep hidrokarbon, absorbsi, larut air dan tercuci air. Salep dievaluasi sifat fisik meliputi uji organoleptis, homogenitas, pH, daya lekat, daya sebar, proteksi dan daya penyembuh luka bakar menggunakan hewan uji marmut. Data yang diperoleh dianalisis menggunakan uji statistika dengan taraf kepercayaan 95%. Hasil uji menunjukkan bahwa urutan basis salep yang memberikan homogenitas yang bagus adalah hidrokarbon, absorbsi dan larut air. Urutan daya sebar terluas adalah hidrokarbon, larut air, absorbsi dan tercuci air. Dari hasil uji daya lekat urutan daya lekat dari yang terlama adalah hidrokarbon, absorbsi, larut air dan tercuci air, sedangkan urutan kecepatan penyembuhan luka bakar dari yang tercepat adalah hidrokarbon, absorbsi, larut air dan tercuci air. Diketahui pula bahwa tipe basis tidak mempengaruhi nilai pH. Berdasarkan hasil tersebut dapat disimpulkan basis salep dari urutan terbaik adalah hidrokarbon, absorbsi, larut air dan tercuci air.

EVALUASI SIFAT FISIK DAN DAYA IRITASI SEDIAAN LOTION MINYAK ATSIRI BUNGA CENGKEH (SYZIQIUM AROMATICUM) DENGAN BERBAGAI VARIASI KONSENTRASI Fadzil Latifah; Nining Sugihartini; Tedjo Yuwono
Majalah Obat Tradisional Vol 21, No 1 (2016)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

In this study, the lotion contained essential oil of clove Syzigium a romaticum which contained eugenol as the active ingredient. Eugenol has been scientifically proven as anti-inflammatory. Because its activity, the study about development of dosage form is needed. Aims of this study was to know the physical properties of spreadibility, adhesivity, pH, viscosity and irritation index of lotion with variation concentration of essential oil.Thisstudywasstartedwithaformulationoflotionwith variationconcentrationofessentialoil5%(FI); 10% (FII); 15% (FIII). Lotion was evaluated physical properties with parameters spreadibility, adhesivity, viscosity and pH. The evaluation of iritation index was done using guinea pig test animals. Data were analyzed statistically with One Way Anova at confidence level of 95% to detect a significant difference between the treatment groups. The results of the study showed the variation consentration did not influence pH the increasing concentration caused a decreasing spredibility P<0.05 and increasing adhesivity P<0.05. The all of concentration did not irritated the skin of guinea pigs.

Anti-inflammatory Activity of Camellia sinensis, l. Extract Cream Combined with Vitamin C as Antioxidant on Croton Oil-induced Inflamation in Male Mice Strain BALB/C Nining Sugihartini; Ratih Saridewi; Ulfa Ramdhani M; Fitri Rahmawanti; Sapto Yuliani; Vivi Sophia
Majalah Obat Tradisional Vol 22, No 2 (2017)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Green tea extract cream contains epigallocatechin gallate (EGCG) as the active ingredient for anti-inflammatory. Epigallocatechin gallate is easyly oxidized and able to reduce its effectivity as an anti-inflammatory. Therefore, an addition of antioxidants in order to increase its stability is required. The purpose of this study was to determine the effect of adding the antioxidant Vitamin C on the effectivity of green tea extract as an anti-inflammatory. This study uses 6 groups of male mice strain BALB/C which were given treatment as follows: normal control, negative control, base cream, green tea extract (0.2%), Vitamin C cream (1%) and green tea extract cream with addition of Vitamin C. The anti-inflammatory activity was evaluated based on the expression of COX-2, inflammatory cells and the thickness of the epidermis in the skin tissue of mice after given crotton oil (0.1%) on the back for the induction of inflammation. After treatment cream for 3 days, mice were sacrificed for histopathological tissue preparations made with hematoxylin eosin staining and immunohistochemistry COX-2. Data were analyzed statistically with one way Anova followed by t-test to determine differences between groups at a significance level of 0.05. The test results indicate that cream of green tea extract is higher in decreasing inflammatory parameters in comparison with cream of Vitamin C, except in the thickness of epidermal parameter. Green tea extract cream with the addition of Vitamin C is higher in reducing inflammatory parameters than cream of green tea extract or cream of Vitamin C. The decline percentage of cells that express COX-2, inflammatory cells and the thickness of the epidermis in the each of groups were cream of green tea extract:57.95%;53.75%;34.83%, cream of Vitamin C:48.76%;34.96%;34.27%, cream of green tea extract and Vitamin C:61,89%;65,54%;46.30%, respectively. Based on the results of this study, it can be concluded that anti-inflammatory activity of green tea extract cream increased due to the addition of 1% vitamin C as an antioxidant.

Co-Authors . Sismindari Achmad Fudholi Achmad Fudholi Achmad Fudholi Achmad Fudholi Aditya Noviadi Rakhmatullah Afriyani, Neti Agung Putu S.P.Kristyawan Aina Fatkhil Haque, Aina Fatkhil Aji Pamungkas Alvionida, Fitra Amraini Amelia Ani Guntarti Any Guntarti Any Guntarti Astryna, Syarifah Yanti Atri Sri Ulandari Auliah Rahmi Latif Azis Ikhsanudin Bayu Herdi Al Huda Deasy Vanda Pertiwi Delia Komala Sari, Delia Komala Desrianti, Cindy Desyandri Desyandri Diah Pratimasari Diyan Sakti Purwanto Diyan Sakti Purwanto Endah Nurlaela Endah Nurlaela Endang Lukitaningsih Erma Pranawati ERMAWATI, DIAN EKA Erna Astuti Erna Astuti Erwin Fauzana Evi Nuryanti Fadzil Latifah Firsty, Girly Risma Fitra Alvionida Fitri Rahmawanti Fitriah Ardiawijianti Iriani Fudholi, Achmad Fudholi, Achmad Gina Lestari Gina Lestari, Gina Girly Risma Firsty Gita Puspita Hafid, Indah Fitria Hana Hanifah Hari Susanti Hari Susanti Heru Sasongko Ichwan Ridwan Rais Iis Wahyuningsih Jannah, Syauqul Kintoko Kintoko Kintoko, Kintoko Kurniawan, Muhammad Fariez Kusuma, Nyoman Rudi Laela Hayu Nurani Laila, Wahyu Kumil Lalu Muhammad Irham Lela Sulastri, Lela Lena Maulina Lena Maulina, Lena M Alif Fajri Marchaban . Miratun Syarifah Mohamad Ikram Muhammad Alfian, Muhammad Muhammad Fariez Kurniawan Muhammad Fariez Kurniawan Muhammad Yusron Maulana El-Yunusi Mukhlishah, Neneng Rachmalia Izzatul Nanik Sulistyani Nina Salamah NINGRUM, TYAS SARI RATNA Novitasari, Putri Rachma Nugrahini, Febrina Nurhayati Nurhayati Nuri Ari Efiana Nurlaela, Endah Olvia Becatami Widyantoro Olvia Becatami Widyantoro, Olvia Becatami Pertiwi, Deasy Vanda Pramono, Suwidjiyo Pramono, Suwidjiyo Puput Herawati Said Hasan Rahmat Hidayat Rahmawati, Desty Restia Raka Sukmabayu W.S Ratih Saridewi Rikadyanti Rikadyanti Rinanda Yeshi Wiradhika Ririn Suharsanti, Ririn Ristiono, Hendi Rosmiyanti, Yanti Rukaya, Benazir Evita Ryan Radix Rahadhian, Muhammad Sapto Yuliani Sapto Yuliani Sapto Yuliani Sismindari . Sismindari Sismindari Sismindari Sismindari Sismindari Sismindari Sismindari, Sismindari Sismindari, Sismindari Siti Athiyah Umaiyah Siti Ayu Marlina Sri Mulyaningsih Sri Mulyaningsih Srimuliani Arbie Suwidjiyo Pramono Suwidjiyo Pramono Suwidjiyo Pramono Suwidjiyo Pramono Suwidjiyo Pramono. Tedjo Yuwono Tedjo Yuwono Tedjo Yuwono Tedjo Yuwono Tedjo Yuwono Tedjo Yuwono Tedjo Yuwono Tedjo Yuwono Teuku Nanda Saifullah, Teuku Nanda Trecya Fujiastuti Trecya Fujiastuti, Trecya Tusilowati, Defita Andri Ulfa Ramdhani M Uswatun Chasanah Vivi Sophia Waliyyin Razan Qanit wardhani, Riska risty Widyantoro, Olvia Becatami Widyasari Putranti Wisnu Kundarto Wuryandari, Tutik Wuryandari, Tutik Yayan Rizikiyan Zaenab Zaenab Zainab Zainab Zainab Zainab Zainab

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