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All Journal Jurnal Kedokteran Brawijaya Majalah Kedokteran Bandung Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Universa Medicina AKSIOLOGIYA : Jurnal Pengabdian Kepada Masyarakat Jurnal Medik Veteriner Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Parasite Science Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary Journal of Pharmaceutical and Sciences. Journal of Fisheries & Marine Jurnal Agro Veteriner
Lilik Maslachah
Department Of Veterinary Basic Medicine, Faculty Of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, 60115. Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Journal : Ovozoa: Journal of Animal Reproduction

 1 
PENGARUH PENAMBAHAN EKSTRAK TEH HIJAU (Camellia sinensis) DALAM BAHAN PENGENCER KUNING TELUR SITRAT TERHADAP KUALITAS SPERMATOZOA DOMBA SAPUDI YANG DISIMPAN PADA SUHU DINGIN Khoirunnisa, Itrah; Susilowati, Suherni; Maslachah, Lilik; Suprayogi, Tri Wahyu; Kurnijasanti, Rochmah; Ratnani, Hermin
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (403.347 KB) | DOI: 10.20473/ovz.v8i2.2019.127-131

Abstract

The purpose of this research was determine the effect and the best concentration of greentea extract in egg yolk citrate diluent for quality Sapudi sheep spermatozoa were measured in motility, viability and intact plasma membrane that was stored on cold temperature. The semen was devided into four groups : egg yolk citrate diluent, 0,05 % green tea extract in egg yolk citrate diluent, 0,1 % green tea extract in egg yolk citrate diluent and 0,15 % green tea extract in egg yolk citrate diluent. Spermatozoa quality was observed day 1, day 2, day 3, day 4 and day 5 after diluent. The data obtained was analyzed with the analysis of variance (ANOVA), followed by multiple range test duncan. The result showed that the higest precentage of motility, viability and intact plasma membran were obtained from 0,05 % green tea extract in egg yolk citrate diluent for 1 day of storage which were 85.83 ± 3.76, 91.16 ± 2.13 and 66.83 ± 6.49. The lowest precentage of motility, viability and intact plasma membrane were obtained from egg yolk citrate diluent for 5 day of storage which were 26.66 ± 6.05, 49.16 ± 6.43 and 26.66 ± 4.32. Conclution of this research was the addition of 0,05 % green tea extract can be used as a diluent could maintain the precentage of spermatozoa quality until 5 days after diluent.
The effect of watermelon (citrullus lanatus) rind ethanolic extract on the number of leydig, sertoli, and spermatogenic cells of rat (rattus novergicus) exposed to heat Pratama, Abrian Panggalih Indra; Susilowati, Suherni; Maslachah, Lilik; Ratnani, Hermin; Suprayogi, Tri Wahyu
Ovozoa: Journal of Animal Reproduction Vol. 10 No. 1 (2021): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i1.2021.7-11

Abstract

High temperatures lead to oxidative stress, which can disturb spermatogenesis process. Watermelon (Citrullus lanatus) peel contain antioxidant expected to compensate oxidative stress due to heat stress exposure. This study aimed to determine the effect of watermelon rind ethanolic extract on the number of Leydig, Sertoli, and spermatogenic cells of rats exposed to heat (40°C). Twenty rats (Rattus norvegicus) were divided randomly into five groups. In the control group (T0) rats were not exposed to heat nor given the watermelon rind extract. T1, T2, T3, and T4 groups were exposed to heat for an hour daily and orally given placebo (1% Na CMC), 100, 200, and 400 mg/kg BW of watermelon rind extract (in 1% Na CMC). Rats were treated for 52 days, and sacrificed for the testicle collection. Hematoxylin-eosin stained histological slides were prepared for the examination of Leydig, Sertoli and spermatogenic cells. The results showed no significant difference (p >0.05) in the average number of Leydig cells in rats among groups. The number of Sertoli cells and spermatogenic cells of rats exposed to heat (T1) was lower than those of the normal rats (T0 group). The dose of watermelon rind ethanolic extract at 200 mg/kg BW (T3 group) and 400 mg/kg BW (T4) increased (p <0.05) the number of Sertoli and spermatogenic cells. It could be concluded that a dose of 400mg/kg BW of watermelon rind ethanolic extract maintained the number of Leydig cells, Sertoli cells, and spermatogenic cells of rats exposed to heat.
Co-Authors Adiana Mutamsari Witaningrum Agustina, Firanda Al Arif, M Anam Al arif, Mohammad Anam Al-Anshori, Akhmad Afifudin Andreas Berny Yulianto, Andreas Berny Arief Budiman Ayu, Zerlinda Dyah Boedi Setiawan Candika, Yossy Imam Chairul A. Nidom Chairul A. Nidom Chairul Anwar Chandra, Evania Haris Cholifah, Siti Chusnul Chusniati, Sri Dadik Rahardjo, Dadik David Mohamad Qadafi Dewi, Aprilia Kurnia Eko Agus Suyono Endang Suprihati Hani Plumeriastuti Hendarti, Gracia Angelina Hermin Ratnani Hernanda, Ary Setya Herry Agoes Hermadi Hidajati, Nove Hidanah, Sri Hidayatik, Nanik Hisyam, Mirza Atikah Madarina Indriyanti, Astrid Ismi, Alif Noviana Kadek Rachmawati Khoirunnisa, Itrah Koestanti S, Emy Kurnijasanti, Rahma Legowo, Djoko Loeki Enggar Fitri Lucia Tri Suwanti, Lucia Tri Luqman, Epy Muhammad Mahendra Pujiyanto Mirino, Mario Navyseal Mochamad Donny Koerniawan Mochamad Lazuardi Muhammad Hambal Munawer Pradana Mustofa Helmi Effendi Nunuk Dyah Retno Lastuti Nusdianto Triakoso Poedji Hastutiek Pratama, Abrian Panggalih Indra Pujiyanto, Mahendra Putri, Anandia Nafisah Raharjo, Hartanto Mulyo Rahmi Sugihartuti, Rahmi Ratna Damayanti Rochmah Kurnijasanti Rusyawardani, Aldis Ingrid Sabdoningrum, Emy Koestanti Saputra, Rakan Mahiid Saraswati, Afif Tasya Sarudji, Suryanie Soeharsono Soeharsono Sri Mulyati Suherni Susilowati Suhud, Mohamad Kharis Sukmanadi, Moh. Sukmanadi, Mohammad Sulaiman, Azhari Athaillah Supranianondo, Koesnoto Suprihati , Endang Supriyadi Suzanita Utama Tacharina, Martia Rani Tatik Hernawati Tintin Sukartini Sukartini, Tintin Sukartini Tita Damayanti Lestari Tri Wahyu Suprayogi Tyaningsih, Wiwiek Ulfah Juniarti Siregar Utomo, Gogik Satrio Margo Widiyatno, Thomas Valentinus Widjiati Widjiati, Widjiati Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Wiwik Misaco Yuniarti Yoes Prijatna Dachlan Yulianna Puspitasari Zhaza Afililla