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All Journal International Journal of Public Health Science (IJPHS) Pendas : Jurnah Ilmiah Pendidikan Dasar Jurnal Keperawatan Silampari Martabe : Jurnal Pengabdian Kepada Masyarakat JPP Jurnal Kesehatan Poltekkes Palembang Jurnal Pembelajaran dan Biologi Nukleus Best Journal (Biology Education, Sains and Technology) Journal of Community Empowerment for Health Jurnal Ners Journal of Telenursing (JOTING) Dharma Raflesia : Jurnal Ilmiah Pengembangan dan Penerapan IPTEKS Journal of Nursing and Public Health (JNPH) Journal of Midwifery Mitra Raflesia (Journal of Health Science) Indonesian Journal of Health Science PubHealth Jurnal Kesehatan Masyarakat Jurnal Pharmacopoeia Health Research Journal of Indonesia Jurnal Kesehatan Metro Sai Wawai Prosiding Seminar Nasional Unimus Jurnal Vokasi Keperawatan (JVK)
: Samwilson Slamet, : Samwilson
Prodi D3 Keperawatan FMIPA Universitas Bengkulu
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Dentistry Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Determination of total alkaloid content in 70% and 96% ethanol extracts of red seaweed (Eucheuma spinosum) obtained by maceration using UV-Vis spect Sindi Arlin Natasya; Dwi Kurnia Putri; Rose Intan Perma Sari; Samwilson Slamet; Putri Mulia; Nurfitriyana Nurfitriyana
Indonesian Journal of Health Science Vol 6 No 3 (2026)
Publisher : PT WIM Solusi Prima

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54957/ijhs.v6i3.2261

Abstract

Red seaweed (Eucheuma spinosum) is one of the marine biological resources containing various secondary metabolites, including alkaloids which possess important biological activities. Differences in ethanol concentration may affect the amount of alkaloid compounds extracted. This study aimed to compare the alkaloid content of 70% ethanol extract and 96% ethanol extract of red seaweed (Eucheuma spinosum) obtained by maceration using the UV-Visible spectrophotometric method. This research was conducted experimentally in the laboratory using the maceration extraction method with 70% and 96% ethanol solvents. Qualitative identification of alkaloids was performed using Mayer, Wagner, and Dragendorff reagents, while quantitative determination was carried out using UV-Visible spectrophotometry with caffeine as the standard at a maximum wavelength of 275 nm. Phytochemical screening results indicated that both 70% and 96% ethanol extracts were positive for alkaloid compounds. The extraction yield of the 70% ethanol extract was 22.945%, whereas the 96% ethanol extract yielded 12.45%. The results of alkaloid determination showed that the 70% ethanol extract contained an average alkaloid level of 5.5 ± 0.22 mg QCE/g, while the 96% ethanol extract contained 5.3 ± 0.10 mg QCE/g. It can be concluded that the 70% ethanol extract produced a slightly higher alkaloid content than the 96% ethanol extract of red seaweed (Eucheuma spinosum).
ANALISIS PENYALAHGUNAAN RHODAMIN B PADA KERUPUK BERWARNA MERAH YANG BEREDAR DI PASAR KOTA BENGKULU DENGAN MENGGUNKAN METODE KLT DAN SPEKTROFOTOMETRI UV-Vis Adellia Giyani Putri; Mulia, Putri; Ikhsan; Slamet, Samwilson; Perma Sari, Rose Intan
Pendas : Jurnal Ilmiah Pendidikan Dasar Vol. 11 No. 02 (2026): Volume 11 No. 2, Juni 2026 Publish
Publisher : Program Studi Pendidikan Guru Sekolah Dasar FKIP Universitas Pasundan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23969/jp.v11i02.51397

Abstract

Rhodamine B is a synthetic dye that is banned in food products because it can cause health problems, such as irritation, liver damage, and potential carcinogenicity. However, Rhodamine B is still being misused in various types of food, particularly red crackers sold on the market. This study aims to investigate the misuse of Rhodamine B and determine its levels in red crackers sold at Bengkulu City Market. The methods used were Thin-Layer Chromatography (TLC) for qualitative analysis and UV-Vis Spectrophotometry for quantitative analysis. A total of 15 samples of red crackers were collected from several markets in Bengkulu City using purposive sampling. The identification results using TLC showed that 4 out of 15 samples tested positive for Rhodamine B, namely samples PM5, PP1, PK3, and PK5. This was indicated by Rf values close to the Rhodamine B standard as well as the appearance of yellow-orange fluorescence when examined under UV light at 254 nm and 365 nm. Quantitative analysis conducted via UV-Vis spectrophotometry at a maximum wavelength of 542.95 nm revealed Rhodamine B concentrations of 0.0036% for sample PM5, 0.0066% for sample PP1, 0.0037% for sample PK3, and 0.0078% for sample PK5. Based on the research findings, it can be concluded that Rhodamine B is still being misused in red crackers sold at Bengkulu City Market, meaning that these products do not meet food safety standards.
UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN DADAP SEREP (ERYTHRINA LITHOSPERMA MIQ) TERHADAP PERTUMBUHAN BAKTERI SALMONELLA TYPHII ATCC 13313 putri wardani, Erlin; Sipriyadi; mulia, Putri; Slamet, Samwilson; danang kurniawan, Tri
Pendas : Jurnal Ilmiah Pendidikan Dasar Vol. 11 No. 02 (2026): Volume 11 Nomor 02, Juni 2026 Published
Publisher : Program Studi Pendidikan Guru Sekolah Dasar FKIP Universitas Pasundan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23969/jp.v11i02.52963

Abstract

The bacterium Salmonella typhii ATCC 13313 causes typhoid fever, which remains a medical problem in Indonesia. To prevent bacterial resistance, the misuse of antibiotics may lead to the use of alternative antibacterial agents derived from natural sources. The dadap serep leaf, or Erythrina lithosperma Miq, is a plant with potential as an antibacterial agent because it contains secondary metabolites such as alkaloids, flavonoids, triterpenoids, and phenols. The objective of this study was to determine the antibacterial properties of a 96% ethanol extract from dadap serep leaves that affect the growth of Salmonella typhii ATCC 13313. The disk diffusion method was used in this study. The maceration method with 96% ethanol was used to prepare the dadap serep leaf extract. Concentrations of 20%, 40%, 60%, 80%, and 100% were tested. Chloramphenicol was used as a positive control, and DMSO was used as a negative control. After 24-hour incubation at 37°C, inhibition zones around the disk paper indicated antibacterial activity. The results of the study show that the ethanol extract of Erythrina lithosperma Miq. leaves has a yield of 13.86% and is capable of inhibiting the growth of Salmonella typhii ATCC 13313. The higher the concentration of the extract, the greater the inhibitory effect it produces. Therefore, the ethanol extract of Erythrina lithosperma Miq. leaves may serve as a natural antibacterial agent against Salmonella typhii ATCC 13313.
Co-Authors Abda Abda Achmad Fickry Faisa Adellia Giyani Putri Aguscik Aguscik Aguscik, Aguscik Aina Fatkhil Haque Aprilatutini, S.Kep.,M.Pd, Titin Ariana, Rika Ashar Muda Lubis Aziza Afrianti Benny Sihombing Camelia Dwi Putri Masrijal Carissa Ingrid Aurelia CHOIRUNNI’MAH, ZELLA Danang Kurniawan, Tri Delfina, Rina Delia Komala Sari, Delia Komala Deni Maryani Dewi, Novita Rahma Dian Handayani Dwi Kurnia Putri Dwi Retnowati Dwifitri, Uthu Encik Putri Ema Komala Esti Sorena Esti Sorena Faisa, Achmad Fickry Fitri, Sabrina Flora, Rostika - Haerawati Idris Hamzah Hasyim Hamzah Hasyim Hidayah, Thoriqul Ikhsan Ikhsan - Ikhsan Ikhsan Ikhsan Ikhsan Ikhsan Ikhsan Ikhsan Ikhsan, Ikhsan - Indah Yuliana Jasmine, Annisah Biancika Maiyulis Maiyulis Maria Eka Patri Y Marissa, Febi Marissa Mashudi Mashudi Masrijal, Camelia Dwi Putri Mauliani Dwi Nathasyah Misnaniarti Misnaniarti Misse Margaret Mohammad - Zulkarnain Monalisa, Shelyn Puteri Muhammad Zulkarnain Mulia, Putri Nabella, Inastia Nabila Aulia Putri Najmah, Najmah Neshy Sulung Neshy Sulung Nori Wirahmi Nova Yustisia Nur Alam Fajar Nur Alam Fajar Nurfitriyana Nurfitriyana Nurlaili - Nurlaili Nurlaili Nurlaili Nurlaili Nurlaili Nurlaili, Nurlaili - Nurmalia Ermi Oky Hermansyah perma sari, rose intan Permasari, Rose Intan Purnama, Yetti Putri Mulia putri wardani, Erlin Rahmad Aswin Juliansyah Rahmadhani, Aliyah Rico Januar Sitorus Risnawati Tanjung Rosaria, Rini Rosaria Rose Intan Perma Sari Roslina Wati Rostika - Flora Rostika Flora Rostika Flora Rostika Flora Rostika Flora Saleh Saleh Sindi Arlin Natasya Sipriyadi Sri Martini Suci Rahmawati Suci Rahmawati, Suci Sulung, Neshy Suryanti Syokuma Wena Taurina, Hilda Tika Hardini Tri Danang Kurniawan Wahid, Muhammad Ali Ashar Wibowo, Risky Hadi Windi Apriandri Wirahmi, Nori Yetti Purnama Yuliana Ardi Yustisia, Nova Zulkarnain, Mohammad Zulkarnain, Mohammad -