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: Samwilson Slamet, : Samwilson
Prodi D3 Keperawatan FMIPA Universitas Bengkulu
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Dentistry Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Determination of total alkaloid content in 70% and 96% ethanol extracts of red seaweed (Eucheuma spinosum) obtained by maceration using UV-Vis spect Sindi Arlin Natasya; Dwi Kurnia Putri; Rose Intan Perma Sari; Samwilson Slamet; Putri Mulia; Nurfitriyana Nurfitriyana
Indonesian Journal of Health Science Vol 6 No 3 (2026)
Publisher : PT WIM Solusi Prima

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54957/ijhs.v6i3.2261

Abstract

Red seaweed (Eucheuma spinosum) is one of the marine biological resources containing various secondary metabolites, including alkaloids which possess important biological activities. Differences in ethanol concentration may affect the amount of alkaloid compounds extracted. This study aimed to compare the alkaloid content of 70% ethanol extract and 96% ethanol extract of red seaweed (Eucheuma spinosum) obtained by maceration using the UV-Visible spectrophotometric method. This research was conducted experimentally in the laboratory using the maceration extraction method with 70% and 96% ethanol solvents. Qualitative identification of alkaloids was performed using Mayer, Wagner, and Dragendorff reagents, while quantitative determination was carried out using UV-Visible spectrophotometry with caffeine as the standard at a maximum wavelength of 275 nm. Phytochemical screening results indicated that both 70% and 96% ethanol extracts were positive for alkaloid compounds. The extraction yield of the 70% ethanol extract was 22.945%, whereas the 96% ethanol extract yielded 12.45%. The results of alkaloid determination showed that the 70% ethanol extract contained an average alkaloid level of 5.5 ± 0.22 mg QCE/g, while the 96% ethanol extract contained 5.3 ± 0.10 mg QCE/g. It can be concluded that the 70% ethanol extract produced a slightly higher alkaloid content than the 96% ethanol extract of red seaweed (Eucheuma spinosum).
Co-Authors Abda Abda Achmad Fickry Faisa Aguscik Aguscik Aguscik, Aguscik Aina Fatkhil Haque Aprilatutini, S.Kep.,M.Pd, Titin Ariana, Rika Ashar Muda Lubis Aziza Afrianti Benny Sihombing Camelia Dwi Putri Masrijal Carissa Ingrid Aurelia CHOIRUNNI’MAH, ZELLA Delfina, Rina Delia Komala Sari Deni Maryani Dewi, Novita Rahma Dian Handayani Dwi Kurnia Putri Dwi Retnowati Dwifitri, Uthu Encik Putri Ema Komala Esti Sorena Esti Sorena Faisa, Achmad Fickry Fitri, Sabrina Flora, Rostika - Haerawati Idris Hamzah Hasyim Hamzah Hasyim Hidayah, Thoriqul Ikhsan - Ikhsan Ikhsan Ikhsan Ikhsan Ikhsan Ikhsan Ikhsan Ikhsan, Ikhsan - Indah Yuliana Jasmine, Annisah Biancika Maiyulis Maiyulis Maria Eka Patri Y Marissa, Febi Marissa Mashudi Mashudi Masrijal, Camelia Dwi Putri Mauliani Dwi Nathasyah Misnaniarti Misnaniarti Misse Margaret Mohammad - Zulkarnain Monalisa, Shelyn Puteri Muhammad Zulkarnain Nabella, Inastia Nabila Aulia Putri Najmah, Najmah Neshy Sulung Neshy Sulung Nori Wirahmi Nova Yustisia Nur Alam Fajar Nur Alam Fajar Nurfitriyana Nurfitriyana Nurlaili - Nurlaili Nurlaili Nurlaili Nurlaili Nurlaili Nurlaili, Nurlaili - Nurmalia Ermi Oky Hermansyah Permasari, Rose Intan Purnama, Yetti Putri Mulia Rahmad Aswin Juliansyah Rahmadhani, Aliyah Rico Januar Sitorus Risnawati Tanjung Rosaria, Rini Rosaria Rose Intan Perma Sari Roslina Wati Rostika - Flora Rostika Flora Rostika Flora Rostika Flora Rostika Flora Saleh Saleh Sindi Arlin Natasya Sri Martini Suci Rahmawati Suci Rahmawati, Suci Sulung, Neshy Suryanti Syokuma Wena Taurina, Hilda Tika Hardini Tri Danang Kurniawan Wahid, Muhammad Ali Ashar Wibowo, Risky Hadi Windi Apriandri Wirahmi, Nori Yetti Purnama Yuliana Ardi Yustisia, Nova Zulkarnain, Mohammad Zulkarnain, Mohammad -